Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,767,140 T→C L53L (TTG→CTG)  rnlB → CP4‑57 prophage; uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,767,1400TC99.2% 475.2 / ‑4.4 122L53L (TTG→CTG) rnlBCP4‑57 prophage; uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (61/60);  total (61/61)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.31e-01

Only 100 of 122 total aligned reads displayed.

CACCGGAATTAACGTCTCAGGGGCTCTAAAAGCTGTCGTTATGGCGACCGGCTTCGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCTTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACGTTCCGACACATATCCTGAGCAAACTGACTTTTACTTTACGTAATC  >  NC_000913/2766995‑2767284
                                                                                                                                                 |                                                                                                                                                
cACCGGAATTAACGTCTCAGGGGCTCTAAAAGCTGTCGTTATGGCGACCGGCTTCGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTgc                                                                                                                                             <  1:601568/151‑1 (MQ=255)
cACCGGAATTAACGTCTCAGGGGCTCTAAAAGCTGTCGTTATGGCGACCGGCTTCGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTgc                                                                                                                                             >  2:550039/1‑151 (MQ=255)
   cGGAATTAACGTCTCAGGGGCTCTAAAAGCTGTCGTTATGGCGACCGGCTTCGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCt                                                                                                                                          >  2:210666/1‑151 (MQ=255)
      aaTTAACGTCTCAGGGGCTCTAAAAGCTGTCGTTATGGCGACCGGCTTCGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAAc                                                                                                                                       <  2:789077/151‑1 (MQ=255)
          aaCGTCTCAGGGGCTCTAAAAGCTGTCGTTATGGCGACCGGCTTCGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTc                                                                                                                                   >  1:637490/1‑151 (MQ=255)
          aaCGTCTCAGGGGCTCTAAAAGCTGTCGTTATGGCGACCGGCTTCGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTc                                                                                                                                   >  2:68147/1‑151 (MQ=255)
            cGTCTCAGGGGCTCTAAAAGCTGTCGTTATGGCGACCGGCTTCGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTcc                                                                                                                                  >  2:908125/1‑150 (MQ=255)
                tcAGGGGCTCTAAAAGCTGTCGTTATGGCGACCGGCTTCGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCagag                                                                                                                              >  1:495040/1‑150 (MQ=255)
                tcAGGGGCTCTAAAAGCTGTCGTTATGGCGACCGGCTTCGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCagag                                                                                                                              >  2:1445107/1‑150 (MQ=255)
                tcAGGGGCTCTAAAAGCTGTCGTTATGGCGACCGGCTTCGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCagag                                                                                                                              >  2:246065/1‑150 (MQ=255)
                 cAGGGGCTCTAAAAGCTGTCGTTATGGCGACCGGCTTCGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTg                                                                                                                            >  2:1062347/1‑151 (MQ=255)
                  aGGGGCTCTAAAAGCTGTCGTTATGGCGACCGGCTTCGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTgg                                                                                                                           >  1:367038/1‑151 (MQ=255)
                   ggggCTCTAAAAGCTGTCGTTATGGCGACCGGCTTCGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTgg                                                                                                                           >  2:308961/1‑150 (MQ=255)
                     ggCTCTAAAAGCTGTCGTTATGGCGACCGGCTTCGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAAc                                                                                                                        <  2:1016536/151‑1 (MQ=255)
                        tctAAAAGCTGTCGTTATGGCGACCGGCTTCGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCg                                                                                                                      >  2:1280266/1‑150 (MQ=255)
                           aaaaGCTGTCGTTATGGCGACCGGCTTCGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCt                                                                                                                    <  1:1435566/149‑1 (MQ=255)
                           aaaaGCTGTCGTTATGGCGACCGGCTTCGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTc                                                                                                                  >  1:1269932/1‑151 (MQ=255)
                           aaaaGCTGTCGTTATGGCGACCGGCTTCGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTc                                                                                                                  >  2:1058747/1‑151 (MQ=255)
                            aaaGCTGTCGTTATGGCGACCGGCTTCGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCg                                                                                                                 <  1:1314970/151‑1 (MQ=255)
                             aaGCTGTCGTTATGGCGACCGGCTTCGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGt                                                                                                                >  2:1390552/1‑151 (MQ=255)
                             aaGCTGTCGTTATGGCGACCGGCTTCGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGt                                                                                                                >  2:669993/1‑151 (MQ=255)
                               gCTGTCGTTATGGCGACCGGCTTCGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGtt                                                                                                               >  2:1567273/1‑150 (MQ=255)
                               gCTGTCGTTATGGCGACCGGCTTCGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTa                                                                                                              <  1:345657/151‑1 (MQ=255)
                                   tCGTTATGGCGACCGGCTTCGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCt                                                                                                          >  1:1239810/1‑151 (MQ=255)
                                    cGTTATGGCGACCGGCTTCGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCt                                                                                                          <  2:1161387/150‑1 (MQ=255)
                                    cGTTATGGCGACCGGCTTCGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCt                                                                                                          <  1:763267/150‑1 (MQ=255)
                                     gTTATGGCGACCGGCTTCGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCTTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTgg                                                                                                        <  1:70776/151‑1 (MQ=255)
                                     gTTATGGCGACCGGCTTCGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTgg                                                                                                        >  1:273929/1‑151 (MQ=255)
                                      ttATGGCGACCGGCTTCGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTgg                                                                                                        >  2:1463464/1‑150 (MQ=255)
                                       tATGGCGACCGGCTTCGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGga                                                                                                       >  2:1186629/1‑150 (MQ=255)
                                               ccGGCTTCGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAata                                                                                              <  1:767134/151‑1 (MQ=255)
                                                    ttCGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGcc                                                                                         >  1:613968/1‑151 (MQ=255)
                                                     tCGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCg                                                                                        >  2:753931/1‑151 (MQ=255)
                                                      cGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCgg                                                                                       >  2:658429/1‑151 (MQ=255)
                                                      cGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCgg                                                                                       <  1:953224/151‑1 (MQ=255)
                                                       gagaACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCgg                                                                                       >  2:1716189/1‑150 (MQ=255)
                                                         gaACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATc                                                                                    >  2:235340/1‑151 (MQ=255)
                                                           aCCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCAt                                                                                  <  2:68166/151‑1 (MQ=255)
                                                            cccTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATg                                                                                 <  1:1415299/151‑1 (MQ=255)
                                                                ttGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCt                                                                               <  2:1669773/149‑1 (MQ=255)
                                                                  gTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGAt                                                                           >  2:730847/1‑151 (MQ=255)
                                                                    ccTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATAc                                                                         >  2:1138185/1‑151 (MQ=255)
                                                                    ccTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATAc                                                                         <  2:1647570/151‑1 (MQ=255)
                                                                      tCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACgg                                                                       >  2:139051/1‑151 (MQ=255)
                                                                        aGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGcc                                                                     >  2:1676622/1‑151 (MQ=255)
                                                                        aGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGcc                                                                     >  2:692598/1‑151 (MQ=255)
                                                                         gTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCa                                                                    <  1:1284876/151‑1 (MQ=255)
                                                                          ttAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCa                                                                    >  2:456312/1‑150 (MQ=255)
                                                                          ttAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCa                                                                    >  2:1416586/1‑150 (MQ=255)
                                                                           tAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCaa                                                                   >  1:65324/1‑150 (MQ=255)
                                                                               gAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAatta                                                              <  2:1678169/151‑1 (MQ=255)
                                                                                aaaTTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAattat                                                             <  1:1590128/151‑1 (MQ=255)
                                                                                 aaTTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAattatt                                                            >  1:1431930/1‑151 (MQ=255)
                                                                                  aTTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATtg                                                           >  2:760907/1‑151 (MQ=255)
                                                                                  aTTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATtg                                                           <  2:1239907/151‑1 (MQ=255)
                                                                                     tagaCGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATtgat                                                         >  1:321107/2‑150 (MQ=255)
                                                                                      agaCGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATtgatga                                                       <  2:1491515/151‑1 (MQ=255)
                                                                                      agaCGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATtgatg                                                        <  1:1445009/150‑1 (MQ=255)
                                                                                       gaCGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAg                                                      <  1:128889/151‑1 (MQ=255)
                                                                                        aCGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGc                                                     <  1:1709635/151‑1 (MQ=255)
                                                                                             aCTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGAc                                                >  2:839241/1‑151 (MQ=255)
                                                                                              cTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACg                                               >  2:1380075/1‑151 (MQ=255)
                                                                                               tttCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACGt                                              >  2:1710828/1‑151 (MQ=255)
                                                                                                ttCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACGtt                                             >  1:796746/1‑151 (MQ=255)
                                                                                                   tGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACGTTCCg                                          >  1:1366828/1‑151 (MQ=255)
                                                                                                    gCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACGTTcc                                           <  1:1482645/149‑1 (MQ=255)
                                                                                                    gCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACGTTc                                            <  1:587033/148‑1 (MQ=255)
                                                                                                    gCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACGTTc                                            <  2:173646/148‑1 (MQ=255)
                                                                                                    gCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACGTTc                                            <  2:612371/148‑1 (MQ=255)
                                                                                                         aCTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACGTTCCGAcaca                                     <  2:1626052/150‑1 (MQ=255)
                                                                                                                aGTGAGACCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACGTTCCGACGCATATCCTGa                             <  1:444541/151‑1 (MQ=255)
                                                                                                                   gagaCCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACGTTCCGACACATATCCTGAGCa                          <  1:1160255/151‑1 (MQ=255)
                                                                                                                     gaCCACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACGTTCCGACACATATCCTGAGCaaa                        <  2:1246373/151‑1 (MQ=255)
                                                                                                                       ccACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACGTTCCGACACATATCCTGAGCAAAc                       <  2:1525295/150‑1 (MQ=255)
                                                                                                                       ccACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACGTTCCGACACATATCCTGAGCAAACt                      >  1:1582359/1‑151 (MQ=255)
                                                                                                                       ccACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACGTTCCGACACATATCCTGAGCAAACt                      >  1:158903/1‑151 (MQ=255)
                                                                                                                       ccACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACGTTCCGACACATATCCTGAGCAAACt                      <  2:1716963/151‑1 (MQ=255)
                                                                                                                        cACTGGTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACGTTCCGACACATATCCTGAGCAAACTg                     >  2:1310228/1‑151 (MQ=255)
                                                                                                                            ggTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACGTTCCGACACATATCCTGAGCAAACTGACtt                 <  1:730988/151‑1 (MQ=255)
                                                                                                                             gTGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACGTTCCGACACATATCCTGAGCAAACTGACttt                <  1:549989/151‑1 (MQ=255)
                                                                                                                              tGAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACGTTCCGACACATATCCTGAGCAAACTGACt                  <  1:392860/148‑1 (MQ=255)
                                                                                                                               gAAATCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACGTTCCGACACATATCCTGAGCAAACTGACtttt               <  2:186687/150‑1 (MQ=255)
                                                                                                                                aaaTCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACGTTCCGACACATATCCTGAGCAAACTGACTtttac             <  1:456274/151‑1 (MQ=255)
                                                                                                                                 aaTCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACGTTCCGACACATATCCTGAGCAAACTGACTtttact            <  1:436212/151‑1 (MQ=255)
                                                                                                                                 aaTCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACGTTCCGACACATATCCTGAGCAAACTGACTtttact            <  2:633120/151‑1 (MQ=255)
                                                                                                                                  aTCCTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACGTTCCGACACATATCCTGAGCAAACTGACTtttactt           <  1:908057/151‑1 (MQ=255)
                                                                                                                                    ccTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACGTTCCGACACATATCCTGAGCAAACTGACTtttacttt          >  1:1285997/1‑150 (MQ=255)
                                                                                                                                     cTTTTTGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACGTTCCGACACATATCCTGAGCAAACTGACTtttactttac        >  2:769081/1‑151 (MQ=255)
                                                                                                                                       ttttGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACGTTCCGACACATATCCTGAGCAAACTGACTTTTACTTTACg       <  2:202032/150‑1 (MQ=255)
                                                                                                                                       ttttGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACGTTCCGACACATATCCTGAGCAAACTGACTTTTACTTTACGt      >  1:690549/1‑151 (MQ=255)
                                                                                                                                        tttGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACGTTCCGACACATATCCTGAGCAAACTGACTTTTACTTTACGt      >  2:1314016/1‑150 (MQ=255)
                                                                                                                                         ttGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACGTTCCGACACATATCCTGAGCAAACTGACTTTTACTTTACGTa     <  2:1189016/150‑1 (MQ=255)
                                                                                                                                         ttGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACGTTCCGACACATATCCTGAGCAAACTGACTTTTACTTTACGTa     <  1:1008484/150‑1 (MQ=255)
                                                                                                                                         ttGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACGTTCCGACACATATCCTGAGCAAACTGACTTTTACTTTACGTa     >  2:900037/1‑150 (MQ=255)
                                                                                                                                          tGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACGTTCCGACACATATCCTGAGCAAACTGACTTTTACTTTACGTaa    <  1:677660/150‑1 (MQ=255)
                                                                                                                                          tGACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACGTTCCGACACATATCCTGAGCAAACTGACTTTTACTTTACGTAat   >  2:302096/1‑151 (MQ=255)
                                                                                                                                          agACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACGTTCCGACACATATCCTGAGCAAACTGACTTTTACTTTACGTAat   >  2:514997/2‑151 (MQ=255)
                                                                                                                                           gACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACGTTCCGACACATATCCTGAGCAAACTGACTTTTACTTTACGTAatc  <  2:614022/151‑1 (MQ=255)
                                                                                                                                           gACCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACGTTCCGACACATATCCTGAGCAAACTGACTTTTACTTTACGTAat   <  2:61097/150‑1 (MQ=255)
                                                                                                                                            aCCTCCTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACGTTCCGACACATATCCTGAGCAAACTGACTTTTACTTTACGTAatc  >  1:1051100/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CACCGGAATTAACGTCTCAGGGGCTCTAAAAGCTGTCGTTATGGCGACCGGCTTCGAGAACCCTTTGTCCTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCTTGTGCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATTATTGATGAGCAGGACGTTCCGACACATATCCTGAGCAAACTGACTTTTACTTTACGTAATC  >  NC_000913/2766995‑2767284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: