Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,852,450 A→G K105K (AAA→AAG hypD → hydrogenase maturation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,852,4500AG97.0% 343.8 / 1.1 99K105K (AAA→AAGhypDhydrogenase maturation protein
Reads supporting (aligned to +/- strand):  ref base A (2/1);  new base G (40/56);  total (42/57)
Fisher's exact test for biased strand distribution p-value = 5.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.03e-01

TTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACTGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAACAGGGATCGCTGTTGCAGGCAAAAGCACGCGGTGCCGATGTGCGCATCGTTTACTCGCCGATGGATGCGTTGAAACTGGCGCAGGAGAATCCAACCCGCAAAGTGGTGTTCTTCGGCTTAGGTTTTGAAACCACTATGCCGACC  >  NC_000913/2852301‑2852594
                                                                                                                                                     |                                                                                                                                                
ttACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACTGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAAc                                                                                                                                                 >  2:1555097/1‑151 (MQ=255)
  aCTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACTGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAGCa                                                                                                                                                <  2:185434/150‑1 (MQ=255)
    tGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACTGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAGCAggg                                                                                                                                             >  2:1319657/1‑151 (MQ=255)
       cGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACTGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAGCAGGGATc                                                                                                                                          <  2:939603/151‑1 (MQ=255)
         gAAAACGTTGAGTTTATCCACGGTCCGAGGTGCCCGGTGTGCGTACTGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCAGCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAGCAGGGATCg                                                                                                                                         <  1:1224469/150‑1 (MQ=255)
          aaaaCGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACTGCCGTTGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAGCAGGGATCGCt                                                                                                                                       <  2:79952/151‑1 (MQ=255)
          aaaaCGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACTGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAGCAGGGATCGCt                                                                                                                                       <  2:1211090/151‑1 (MQ=255)
          aaaaCGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACTGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAGCAGGGATCGCt                                                                                                                                       >  2:1637010/1‑151 (MQ=255)
          aaaaCGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACTGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAGCAGGGATCGCt                                                                                                                                       <  2:1693930/151‑1 (MQ=255)
           aaaCGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACTGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAGCAGGGATCGCTg                                                                                                                                      <  2:739669/151‑1 (MQ=255)
             aCGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACTGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAGCAGGGATCGCTGtt                                                                                                                                    <  2:1111552/151‑1 (MQ=255)
             aCGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACTGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAGCAGGGATCGCTGtt                                                                                                                                    >  2:212415/1‑151 (MQ=255)
             aCGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACTGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAGCAGGGATCGCTGtt                                                                                                                                    <  1:787686/151‑1 (MQ=255)
                   aGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACTGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAGCAGGGATCGCTGTTGCAGGc                                                                                                                              >  1:1601001/1‑151 (MQ=255)
                   aGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACTGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAGCAGGGATCGCTGTTGCAGGc                                                                                                                              >  1:455802/1‑151 (MQ=255)
                      ttATCCACGGTCCGGGGTGCCCGGTGTGCGTACTGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAGCAGGGATCGCTGTTGCAGGCaa                                                                                                                            <  2:714063/150‑1 (MQ=255)
                       tATCCACGGTCCGGGGTGCCCGGTGTGCGTACTGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAGCAGGGATCGCTGTTGCAGGCaa                                                                                                                            <  1:892662/149‑1 (MQ=255)
                          ccACGGTCCGGGGTGCCCGGTGTGCGTACTGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAGCAGGGATCGCTGTTGCAGGCAAAAGc                                                                                                                        >  1:449943/1‑150 (MQ=255)
                          ccACGGTCCGGGGTGCCCGGTGTGCGTACTGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAGCAGGGATCGCTGTTGCAGGCAAAAGCa                                                                                                                       <  1:888166/151‑1 (MQ=255)
                           cACGGTCCGGGGTGCCCGGTGTGCGTACTGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAGCAGGGATCGCTGTTGCAGGCAAAAGCAc                                                                                                                      <  1:502542/151‑1 (MQ=255)
                             cGGTCCGGGGTGCCCGGTGTGCGTACTGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAGCAGGGATCGCTGTTGCAGGCAAAAGCAcgc                                                                                                                    <  2:603137/151‑1 (MQ=255)
                             cGGTCCGGGGTGCCCGGTGTGCGTACTGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAGCAGGGATCGCTGTTGCAGGCAAAAGCAcgc                                                                                                                    <  2:272649/151‑1 (MQ=255)
                              ggTCCGGGGTGCCCGGTGTGCGTACTGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAGCAGGGATCGCTGTTGCAGGCAAAAGCAcgcg                                                                                                                   >  2:1347202/1‑151 (MQ=255)
                              ggTCCGGGGTGCCCGGTGTGCGTACTGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAGCAGGGATCGCTGTTGCAGGCAAAAGCAcgcg                                                                                                                   <  1:122604/151‑1 (MQ=255)
                              ggTCCGGGGTGCCCGGTGTGCGTACTGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGCACCTTTGGCGACGCCATGCGCGTGCCGGGGAAGCAGGGATCGCTGTTGCAGGCAAAAGCAcgcg                                                                                                                   <  1:898903/151‑1 (MQ=255)
                                  cGGGGTGCCCGGTGTGCGTACTGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAGCAGGGATCGCTGTTGCAGGCAAAAGCACGCGGTg                                                                                                                >  1:1713063/1‑150 (MQ=255)
                                    gggTGCCCGGTGTGCGTACTGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAGCAGGGATCGCTGTTGCAGGCAAAAGCACGCGGTGCCg                                                                                                             <  1:1509043/151‑1 (MQ=255)
                                       tGCCCGGTGTGCGTACTGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAGCAGGGATCGCTGTTGCAGGCAAAAGCACGCGGTGCCGAtg                                                                                                          >  1:600595/1‑151 (MQ=255)
                                         cccGGTGTGCGTACTGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAGCAGGGATCGCTGTTGCAGGCAAAAGCACGCGGTGCCGAtgtg                                                                                                        <  2:340580/151‑1 (MQ=255)
                                         cccGGTGTGCGTACTGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAGCAGGGATCGCTGTTGCAGGCAAAAGCACGCGGTGCCGAtgtg                                                                                                        <  1:445066/151‑1 (MQ=255)
                                           cGGTGTGCGTACTGTCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAGCAGGGATCGCTGTTGCAGGCAAAAGCACGCGGTGCCGATGTgcg                                                                                                      <  2:502090/151‑1 (MQ=255)
                                            ggTGTGCGTACTGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAGCAGGGATCGCTGTTGCAGGCAAAAGCACGCGGTGCCGATGTgcgc                                                                                                     >  1:172800/1‑151 (MQ=255)
                                                  cGTACTGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAGCAGGGATCGCTGTTGCAGGCAAAAGCACGCGGTGCCGATGTGCGCATCGtt                                                                                               >  2:775778/1‑151 (MQ=255)
                                                   gTACTGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAGCAGGGATCGCTGTTGCAGGCAAAAGCACGCGGTGCCGATGTGCGCATCGttt                                                                                              >  1:1013138/1‑151 (MQ=255)
                                                      cTGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAGCAGGGATCGCTGTTGCAGGCAAAAGCACGCGGTGCCGATGTGCGCAt                                                                                                   <  1:884223/143‑1 (MQ=255)
                                                        gCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAGCAGGGATCGCTGTTGCAGGCAAAAGCACGCGGTGCCGATGTGCGCATCGTTTACt                                                                                           <  1:746075/149‑1 (MQ=255)
                                                        gCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAGCAGGGATCGCTGTTGCAGGCAAAAGCACGCGGTGCCGATGTGCGCATCGTTTACt                                                                                           <  1:1148585/149‑1 (MQ=255)
                                                        gCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAGCAGGGATCGCTGTTGCAGGCAAAAGCACGCGGTGCCGATGTGCGCATCGTTTACTCg                                                                                         <  2:302012/151‑1 (MQ=255)
                                                          cGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAGCAGGGATCGCTGTTGCAGGCAAAAGCACGCGGTGCCGATGTGCGCATCGTTTACTCGcc                                                                                       >  2:281111/1‑151 (MQ=255)
                                                              gggTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAGCAGGGATCGCTGTTGCAGGCAAAAGCACGCGGTGCCGATGTGCGCATCGTTTACTCGCCgatg                                                                                   >  2:350603/1‑151 (MQ=255)
                                                              gggTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAGCAGGGATCGCTGTTGCAGGCAAAAGCACGCGGTGCCGATGTGCGCATCGTTTACTCGCCgatg                                                                                   >  2:1360272/1‑151 (MQ=255)
                                                                gTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAGCAGGGATCGCTGTTGCAGGCAAAAGCACGCGGTGCCGATGTGCGCATCGTTTACTCGCCgatgga                                                                                 <  2:1163914/151‑1 (MQ=255)
                                                                   gAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAGCAGGGATCGCTGTTGCAGGCAAAAGCACGCGGTGCCGATGTGCGCATCGTTTACTCGCCGATGGATGc                                                                              >  2:96018/1‑151 (MQ=255)
                                                                   gAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAACAGGGATCGCTGTTGCAGGCAAAAGCACGCGGTGCCGATGTGCGCATCGTTTACTCGCCGATGGATGc                                                                              >  1:1411099/1‑151 (MQ=255)
                                                                     aTCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAGCAGGGATCGCTGTTGCAGGCAAAAGCACGCGGTGCCGATGTGCGCATCGTTTACTCGCCGATGGATGCGt                                                                            <  1:133378/151‑1 (MQ=255)
                                                                      tCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAGCAGGGATCGCTGTTGCAGGCAAAAGCACGCGGTGCCGATGTGCGCATCGTTTACTCGCCGATGGATGCGtt                                                                           >  2:343479/1‑151 (MQ=255)
                                                                       cGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAGCAGGGATCGCTGTTGCAGGCAAAAGCACGCGGTGCCGATGTGCGCATCGTTTACTCGCCGATGGATGCGtt                                                                           <  1:1700309/150‑1 (MQ=255)
                                                                       cGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAGCAGGGATCGCTGTTGCAGGCAAAAGCACGCGGTGCCGATGTGCGCATCGTTTACTCGCCGATGGATGCGtt                                                                           <  2:910826/150‑1 (MQ=255)
                                                                       cGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAGCAGGGATCGCTGTTGCAGGCAAAAGCACGCGGTGCCGATGTGCGCATCGTTTACTCGCCGATGGATGCGTTg                                                                          <  2:1646721/151‑1 (MQ=255)
                                                                         acacCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAGCAGGGATCGCTGTTGCAGGCAAAAGCACGCGGTGCCGATGTGCGCATCGTTTACTCGCCGATGGATGCGTTGaa                                                                        <  2:1182920/151‑1 (MQ=255)
                                                                           acCTGCGTGGAGATTGCAAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAGCAGGGATCGCTGTTGCAGGCAAAAGCACGCGGTGCCGATGTGCGCATCGTTTACTCGCCGATGGATGCGTTGaa                                                                        <  1:1416070/149‑1 (MQ=255)
                                                                            ccTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAGCAGGGATCGCTGTTGCAGGCAAAAGCACGCGGTGCCGATGTGCGCATCGTTTACTCGCCGATGGATGCGTTGAAAc                                                                      <  1:1536102/150‑1 (MQ=255)
                                                                            ccTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAGCAGGGATCGCTGTTGCAGGCAAAAGCACGCGGTGCCGATGTGCGCATCGTTTACTCGCCGATGGATGCGTTGAAACt                                                                     <  1:922596/151‑1 (MQ=255)
                                                                            ccTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAGCAGGGATCGCTGTTGCAGGCAAAAGCACGCGGTGCCGATGTGCGCATCGTTTACTCGCCGATGGATGCGTTGAAACt                                                                     >  2:417677/1‑151 (MQ=255)
                                                                             cTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAGCAGGGATCGCTGTTGCAGGCAAAAGCACGCGGTGCCGATGTGCGCATCGTTTACTCGCCGATGGATGCGTTGAAACTg                                                                    >  2:614344/1‑151 (MQ=255)
                                                                              tGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAGCAGGGATCGCTGTTGCAGGCAAAAGCACGCGGTGCCGATGTGCGCATCGTTTACTCGCCGATGGATGCGTTGAAACTgg                                                                   >  1:737930/1‑151 (MQ=255)
                                                                               gCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAGCAGGGATCGCTGTTGCAGGCAAAAGCACGCGGTGCCGATGTGCGCATCGTTTACTCGCCGATGGATGCGTTGAAACTg                                                                    <  2:1014523/149‑1 (MQ=255)
                                                                               gCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAGCAGGGATCGCTGTTGCAGGCAAAAGCACGCGGTGCCGATGTGCGCATCGTTTACTCGCCGATGGATGCGTTGAAACTGgc                                                                  <  1:564638/151‑1 (MQ=255)
                                                                                 gTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAGCAGGGATCGCTGTTGCAGGCAAAAGCACGCGGTGCCGATGTGCGCATCGTTTACTCGCCGATGGATGCGTTGAAACTGgcgc                                                                <  2:1305087/151‑1 (MQ=255)
                                                                                 gTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAGCAGGGATCGCTGTTGCAGGCAAAAGCACGCGGTGCCGATGTGCGCATCGTTTACTCGCCGATGGATGCGTTGAAACTGgcg                                                                 <  1:1617508/150‑1 (MQ=255)
                                                                                  tGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAGCAGGGATCGCTGTTGCAGGCAAAAGCACGCGGTGCCGATGTGCGCATCGTTTACTCGCCGATGGATGCGTTGAAACTGgcgc                                                                >  1:738357/1‑150 (MQ=255)
                                                                                   ggATATTGCTAGCCGCACGGAAGTCATCTTCTGTCTCTTTGGCGACGCCATGCGCGTGCCGGGGAAGCAGGGATCTCTGTTGCAGGGAAAAGCACGCGGTGCCGATCTGCGCCTCGTTTACTCGCCGATGGATGCGTTGAACCTGGCGCa                                                               <  1:386197/150‑1 (MQ=255)
                                                                                    gagaTTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAGCAGGGATCGCTGTTGCAGGCAAAAGCACGCGGTGCCGATGTGCGCATCGTTTACTCGCCGATGGATGCGTTGAAACTGGCGCAgg                                                             >  1:58929/1‑151 (MQ=255)
                                                                                    gagaTTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAGCAGGGATCGCTGTTGCAGGCAAAAGCACGCGGTGCCGATGTGCGCATCGTTTACTCGCCGATGGATGCGTTGAAACTGGCGCAg                                                              <  2:1713179/150‑1 (MQ=255)
                                                                                     agaTTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAGCAGGGATCGCTGTTGCAGGCAAAAGCACGCGGTGCCGATGTGCGCATCGTTTACTCGCCGATGGATGCGTTGAAACTGGCGCAgg                                                             >  1:1126218/1‑150 (MQ=255)
                                                                                        ttGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAGCAGGGATCGCTGTTGCAGGCAAAAGCACGCGGTGCCGATGTGCGCATCGTTTACTCGCCGATGGATGCGTTGAAACTGGCGCAGgaga                                                          >  2:1074507/1‑150 (MQ=255)
                                                                                        ttGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAGCAGGGATCGCTGTTGCAGGCAAAAGCACGCGGTGCCGATGTGCGCATCGTTTACTCGCCGATGGATGCGTTGAAACTGGCGCAGgaga                                                          >  2:1110529/1‑150 (MQ=255)
                                                                                         tGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAGCAGGGATCGCTGTTGCAGGCAAAAGCACGCGGTGCCGATGTGCGCATCGTTTACTCGCCGATGGATGCGTTGAAACTGGCGCAGGAGaa                                                         <  1:195908/150‑1 (MQ=255)
                                                                                         tGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAGCAGGGATCGCTGTTGCAGGCAAAAGCACGCGGTGCCGATGTGCGCATCGTTTACTCGCCGATGGATGCGTTGAAACTGGCGCAGGAGAAt                                                        >  1:1165016/1‑151 (MQ=255)
                                                                                          gccagccaTCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAGCAGGGATCGCTGTTGCAGGCAAAAGCACGCGGTGCCGATGTGCGCATCGTTTACTCGCCGATGGATGCGTTGAAACTGGCGCAGGAGAATc                                                       <  2:1516977/151‑1 (MQ=255)
                                                                                            cagccaTCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAGCAGGGATCGCTGTTGCAGGCAAAAGCACGCGGTGCCGATGTGCGCATCGTTTACTCGCCGATGGATGCGTTGAAACTGGCGCAGGAGAATcc                                                      >  1:619857/1‑150 (MQ=255)
                                                                                            cagccaTCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAGCAGGGATCGCTGTTGCAGGCAAAAGCACGCGGTGCCGATGTGCGCATCGTTTACTCGCCGATGGATGCGTTGAAACTGGCGCAGGAGAATCCa                                                     <  2:452231/151‑1 (MQ=255)
                                                                                              gccaTCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAGCAGGGATCGCTGTTGCAGGCAAAAGCACGCGGTGCCGATGTGCGCATCGTTTACTCGCCGATGGATGCGTTGAAACTGGCGCAGGAGAATCCaa                                                    <  1:511277/150‑1 (MQ=255)
                                                                                                caTCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAGCAGGGATCGCTGTTGCAGGCAAAAGCACGCGGTGCCGATGTGCGCATCGTTTACTCGCCGATGGATGCGTTGAAACTGGCGCAGGAGAATCCAAccc                                                 >  2:64782/1‑151 (MQ=255)
                                                                                                caTCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAGCAGGGATCGCTGTTGCAGGCAAAAGCACGCGGTGCCGATGTGCGCATCGTTTACTCGCCGATGGATGCGTTGAAACTGGCGCAGGAGAATCCAAccc                                                 >  2:51232/1‑151 (MQ=255)
                                                                                                 aTCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAGCAGGGATCGCTGTTGCAGGCAAAAGCACGCGGTGCCGATGTGCGCATCGTTTACTCGCCGATGGATGCGTTGAAACTGGCGCAGGAGAATCCAACCCg                                                <  1:1546223/151‑1 (MQ=255)
                                                                                                  tCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAGCAGGGATCGCTGTTGCAGGCAAAAGCACGCGGTGCCGATGTGCGCATCGTTTACTCGCCGATGGATGCGTTGAAACTGGCGCAGGAGAATCCAACCCGc                                               >  1:229433/1‑151 (MQ=255)
                                                                                                    cGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGTGAAGCAGGGATCGCTGTTGCAGGCAAAAGCACGCGGTGCCGTTGTGCGCATCGTTTACTCGCCGATGGATGCGTTGAAACTGGCGCAGGAGAATCCAACCCGCaa                                             <  2:213439/151‑1 (MQ=255)
                                                                                                    cGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAGCAGGGATCGCTGTTGCAGGCAAAAGCACGCGGTGCCGATGTGCGCATCGTTTACTCGCCGATGGATGCGTTGAAACTGGCGCAGGAGAATCCAACCCGCa                                              >  1:312767/1‑150 (MQ=255)
                                                                                                     ggAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAGCAGGGATCGCTGTTGCAGGCAAAAGCACGCGGTGCCGATGTGCGCATCGTTTACTCGCCGATGGATGCGTTGAAACTGGCGCAGGAGAATCCAACCCGCaaa                                            <  2:455840/151‑1 (MQ=255)
                                                                                                      gAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAGCAGGGATCGCTGTTGCAGGCAAAAGCACGCGGTGCCGATGTGCGCATCGTTTACTCGCCGATGGATGCGTTGAAACTGGCGCAGGAGAATCCAACCCGCAAAg                                           <  2:705320/151‑1 (MQ=255)
                                                                                                            aTCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAGCAGGGATCGCTGTTGCAGGCAAAAGCACGCGGTGCCGATGTGCGCATCGTTTACTCGCCGATGGATGCGTTGAAACTGGCGCAGGAGAATCCAACCCGCAAAGTGgtgt                                     >  1:20356/1‑151 (MQ=255)
                                                                                                               ttctGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAGCAGGGATCGCTGTTGCAGGCAAAAGCACGCGGTGCCGATGTGCGCATCGTTTACTCGCCGATGGATGCGTTGAAACTGGCGCAGGAGAATCCAACCCGCAAAGTGGTGttct                                  >  2:175920/1‑151 (MQ=255)
                                                                                                                tctGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAGCAGGGATCGCTGTTGCAGGCAAAAGCACGCGGTGCCGATGTGCGCATCGTTTACTCGCCGATGGATGCGTTGAAACTGGCGCAGGAGAATCCAACCCGCAAAGTGGTGttct                                  <  2:1615298/150‑1 (MQ=255)
                                                                                                                tctGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAGCAGGGATCGCTGTTGCAGGCAAAAGCACGCGGTGCCGATGTGCGCATCGTTTACTCGCCGATGGATGCGTTGAAACTGGCGCAGGAGAATCCAACCCGCAAAGTGGTGtt                                    <  2:1478446/148‑1 (MQ=255)
                                                                                                                   gTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAGCAGGGATCGCTGTTGCAGGCAAAAGCACGCGGTGCCGATGTGCGCATCGTTTACTCGCCGATGGATGCGTTGAAACTGGCGCAGGAGAATCCAACCCGCAAAGTGGTGttctt                                 <  1:204332/148‑1 (MQ=255)
                                                                                                                         ttGGCGACGCCATGCGCGTGCCGGGGAAGCAGGGATCGCTGTTGCAGGCAAAAGCACGCGGTGCCGATGTGCGCATCGTTTACTCGCCGATGGATGCGTTGAAACTGGCGCAGGAGAATCCAACCCGCAAAGTGGTGTTCTTCGGCTTAg                         <  1:1519882/150‑1 (MQ=255)
                                                                                                                            gCGACGCCATGCGCGTGCCGGGGAAGCAGGGATCGCTGTTGCAGGCAAAAGCACGCGGTGCCGATGTGCGCATCGTTTACTCGCCGATGGATGCGTTGAAACTGGCGCAGGAGAATCCAACCCGCAAAGTGGTGTTCTTCGGCTTAGGttt                     <  1:14682/151‑1 (MQ=255)
                                                                                                                             cGACGCCATGCGCGTGCCGGGGAAGCAGGGATCGCTGTTGCAGGCAAAAGCACGCGGTGCCGATGTGCGCATCGTTTACTCGCCGATGGATGCGTTGAAACTGGCGCAGGAGAATCCAACCCGCAAAGTGGTGTTCTTCGGCTTAGGtttt                    >  1:1454851/1‑151 (MQ=255)
                                                                                                                                cGCCATGCGCGTGCCGGGGAAGCAGGGATCGCTGTTGCAGGCAAAAGCACGCGGTGCCGATGTGCGCATCGTTTACTCGCCGATGGATGCGTTGAAACTGGCGCAGGAGAATCCAACCCGCAAAGTGGTGTTCTTCGGCTTAGGTTTTGaa                 <  2:229454/151‑1 (MQ=255)
                                                                                                                                   cATGCGCGTGCCGGGGAAGCAGGGATCGCTGTTGCAGGCAAAAGCACGCGGTGCCGATGTGCGCATCGTTTACTCGCCGATGGATGCGTTGAAACTGGCGCAGGAGAATCCAACCCGCAAAGTGGTGTTCTTCGGCTTAGGTTTTGAAAcc              <  2:738415/151‑1 (MQ=255)
                                                                                                                                     tGCGCGTGCCGGGGAAGCAGGGATCGCTGTTGCAGGCAAAAGCACGCGGTGCCGATGTGCGCATCGTTTACTCGCCGATGGATGCGTTGAAACTGGCGCAGGAGAATCCAACCCGCAAAGTGGTGTTCTTCGGCTTAGGTTTTGAAACCa             <  1:317740/150‑1 (MQ=255)
                                                                                                                                      gcgcGTGCCGGGGAAGCAGGGATCGCTGTTGCAGGCAAAAGCACGCGGTGCCGATGTGCGCATCGTTTACTCGCCGATGGATGCGTTGAAACTGGCGCAGGAGAATCCAACCCGCAAAGTGGTGTTCTTCGGCTTAGGTTTTGAAACCACt           >  1:228648/1‑151 (MQ=255)
                                                                                                                                       cgcgTGCCGGGGAAGCAGGGATCGCTGTTGCAGGCAAAAGCACGCGGTGCCGATGTGCGCATCGTTTACTCGCCGATGGATGCGTTGAAACTGGCGCAGGAGAATCCAACCCGCAAAGTGGTGTTCTTCGGCTTAGGTTTTGAAACCACt           >  1:232137/1‑150 (MQ=255)
                                                                                                                                          gTGCCGGGGAAGCAGGGATCGCTGTTGCAGGCAAAAGCACGCGGTGCCGATGTGCGCATCGTTTACTCGCCGATGGATGCGTTGAAACTGGCGCAGGAGAATCCAACCCGCAAAGTGGTGTTCTTCGGCTTAGGTTTTGAAACCACTATGc       >  2:1264867/1‑151 (MQ=255)
                                                                                                                                           tGCCGGGGAAGCAGGGATCGCTGTTGCAGGCAAAAGCACGCGGTGCCGATGTGCGCATCGTTTACTCGCCGATGGATGCGTTGAAACTGGCGCAGGAGAATCCAACCCGCAAAGTGGTGTTCTTCGGCTTAGGTTTTGAAACCACTATGcc      >  1:1647105/1‑151 (MQ=255)
                                                                                                                                              cGGGGAAGCAGGGATCGCTGTTGCAGGCAAAAGCACGCGGTGCCGATGTGCGCATCGTTTACTCGCCGATGGATGCGTTGAAACTGGCGCAGGAGAATCCAACCCGCAAAGTGGTGTTCTTCGGCTTAGGGTTTGAAACCACTATGCCGac   >  2:37887/1‑151 (MQ=255)
                                                                                                                                               ggggAAACAGGGATCGCTGTTGCAGGCAAAAGCACGCGGTGCCGATGTGCGCATCGTTTACTCGCCGATGGATGCGTTGAAACTGGCGCAGGAGAATCCAACCCGCAAAGTGGTGTTCTTCGGCTTAGGTTTTGAAACCACTATGCCGacc  <  2:1678754/151‑1 (MQ=255)
                                                                                                                                                gggAAGCAGGGATCGCTGTTGCAGGCAAAAGCACGCGGTGCCGATGTGCGCATCGTTTACTCGCCGATGGATGCGTTGAAACTGGCGCAGGAGAATCCAACCCGCAAAGTGGTGTTCTTCGGCTTAGGTTTTGAAACCACTATGCCGacc  >  1:343994/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                
TTACTGCCGGAAAACGTTGAGTTTATCCACGGTCCGGGGTGCCCGGTGTGCGTACTGCCGATGGGTAGAATCGACACCTGCGTGGAGATTGCCAGCCATCCGGAAGTCATCTTCTGTACCTTTGGCGACGCCATGCGCGTGCCGGGGAAACAGGGATCGCTGTTGCAGGCAAAAGCACGCGGTGCCGATGTGCGCATCGTTTACTCGCCGATGGATGCGTTGAAACTGGCGCAGGAGAATCCAACCCGCAAAGTGGTGTTCTTCGGCTTAGGTTTTGAAACCACTATGCCGACC  >  NC_000913/2852301‑2852594

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: