Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,858,154 T→C R354R (CGT→CGC mutS → methyl‑directed mismatch repair protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,858,1540TC96.2% 368.5 / 3.8 104R354R (CGT→CGCmutSmethyl‑directed mismatch repair protein
Reads supporting (aligned to +/- strand):  ref base T (3/1);  new base C (48/52);  total (51/53)
Fisher's exact test for biased strand distribution p-value = 3.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.89e-01

Only 100 of 104 total aligned reads displayed.

TATGCTGAAACGCTGGCTGCATATGCCAGTGCGCGATACCCGCGTGTTGCTTGAGCGCCAGCAAACTATTGGCGCATTGCAGGATTTCACCGCCGGGCTACAGCCGGTACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGC  >  NC_000913/2858007‑2858302
                                                                                                                                                   |                                                                                                                                                    
tATGCTGAAACGCTGGCTGCATATGCCAGTGCGCGATACCCGCGTGTTGCTTGAGCGCCAGCAAACTATTGGCGCATTGCAGGATTTCACCGCCGGGCTACAGCCGGTACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGCCTg                                                                                                                                                   >  2:1566201/1‑151 (MQ=255)
tATGCTGAAACGCTGGCTGCATATGCCAGTGCGCGATACCCGCGTGTTGCTTGAGCGCCAGCAAACTATTGGCGCATTGCAGGATTTCACCGCCGGGCTACAGCCGGTACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGCCTg                                                                                                                                                   >  1:282635/1‑151 (MQ=255)
      gAAACGCTGGCTGCATATGCCAGTGCGCGATACCCGCGTGTTGCTTGAGCGCCAGCAAACTATTGGCGCATTGCAGGATTTCACCGCCGGGCTACAGCCGGTACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGCCTGGCTTTa                                                                                                                                             <  1:868680/151‑1 (MQ=255)
       aaaCGCTGGCTGCATATGCCAGTGCGCGATACCCGCGTGTTGCTTGAGCGCCAGCAAACTATTGGCGCATTGCAGGATTTCACCGCCGGGCTACAGCCGGTACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGCCTGGCTTTAc                                                                                                                                            >  2:1228202/1‑151 (MQ=255)
        aaCGCTGGCTGCATATGCCAGTGCGCGATACCCGCGTGTTGCTTGAGCGCCAGCAAACTATTGGCGCATTGCAGGATTTCACCGCCGGGCTACAGCCGGTACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGCCTGGCTTTACg                                                                                                                                           <  2:1165622/151‑1 (MQ=255)
          cGCTGGCTGCATATGCCAGTGCGCGATACCCGCGTGTTGCTTGAGCGCCAGCAAACTATTGGCGCATTGCAGGATTTCACCGCCGGGCTACAGCCGGTACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGCCTGGCTTTACGaa                                                                                                                                         <  1:717802/151‑1 (MQ=255)
          cGCTGGCTGCATATGCCAGTGCGCGATACCCGCGTGTTGCTTGAGCGCCAGCAAACTATTGGCGCATTGCAGGATTTCACCGCCGGGCTACAGCCGGTACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGCCTGGCTTTACGa                                                                                                                                          <  1:122658/150‑1 (MQ=255)
          cGCTGGCTCCATATGCCAGTGCGCGATACCCGCGTGTTGCTTGAGCGCCAGCAAACTATTGGCGCATTGCAGGATTTCACCGCCGGGCTACAGCCGGTACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGCCTGGCTTTACGa                                                                                                                                          <  1:9771/150‑1 (MQ=255)
                 tgCATATGCCAGTGCGCGATACCCGCGTGTTGCTTGAGCGCCAGCAAACTATTGGCGCATTGCAGGATTTCACCGCCGGGCTACAGCCGGTACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGCCTGGCTTTACGAACTGCTCg                                                                                                                                  >  1:756265/1‑151 (MQ=255)
                  gCATATGCCAGTGCGCGATACCCGCGTGTTGCTTGAGCGCCAGCAAACTATTGGCGCATTGCAGGATTTCACCGCCGGGCTACAGCCGGTACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGCCTGGCTTTACGAACTGCTCGc                                                                                                                                 <  2:444308/151‑1 (MQ=255)
                    atatGCCAGTGCGCGATCCCCGCGTGTTGCTTGAGCGCCAGCAAACTATTGGCGCATTGCAGGATTTCACCGCCGGGCTACAGCCGGTACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGCCTGGCTTTACGAACTGCTCGcc                                                                                                                                <  1:732355/150‑1 (MQ=255)
                       tGCCAGTGCGCGATACCCGCGTGTTGCTTGAGCGCCAGCAAACTATTGGCGCATTGCAGGATTTCACCGCCGGGCTACAGCCGGTACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGCCTGGCTTTACGAACTGCTCGCCCAc                                                                                                                             <  1:17290/150‑1 (MQ=255)
                         ccAGTGCGCGATACCCGCGTGTTGCTTGAGCGCCAGCAAACTATTGGCGCATTGCAGGATTTCACCGCCGGGCTACAGCCGGTACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGCCTGGCTTTACGAACTGCTCGCCCAcgcg                                                                                                                          >  1:1331558/1‑151 (MQ=255)
                           aGTGCGCGATACCCGCGTGTTGCTTGAGCGCCAGCAAACTATTGGCGCATTGCAGGATTTCACCGCCGGGCTACAGCCGGTACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGCCTGGCTTTACGAACTGCTCGCCCACGCGAt                                                                                                                        >  2:1169196/1‑151 (MQ=255)
                                 cgATACCCGCGTGTTGCTTGAGCGCCAGCAAACTATTGGCGCATTGCAGGATTTCACCGCCGGGCTACAGCCGGTACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGCCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGcc                                                                                                                  >  1:1487273/1‑151 (MQ=255)
                                  gATACCCGCGTGTTGCTTGAGCGCCAGCAAACTATTGGCGCATTGCAGGATTTCACCGCCGGGCTACAGCCGGTACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGCCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGccc                                                                                                                 <  2:951287/151‑1 (MQ=255)
                                  gATACCCGCGTGTTGCTTGAGCGCCAGCAAACTATTGGCGCATTGCAGGATTTCACCGCCGGGCTACAGCCGGTACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGCCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGccc                                                                                                                 >  2:446924/1‑151 (MQ=255)
                                   aTACCCGCGTGTTGCTTGAGCGCCAGCAAACTATTGGCGCATTGCAGGATTTCACCGCCGGGCTACAGCCGGTACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGCCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCg                                                                                                                <  2:896604/151‑1 (MQ=255)
                                     aCCCGCGTGTTGCTTGAGCGCCAGCAAACTATTGGCGCATTGCAGGATTTCACCGCCGGGCTACAGCCGGTACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGCCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGt                                                                                                               >  1:1375348/1‑150 (MQ=255)
                                     aCCCGCGTGTTGCTTGAGCGCCAGCAAACTATTGGCGCATTGCAGGATTTCACCGCCGGGCTACAGCCGGTACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGCCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGt                                                                                                               >  2:821649/1‑150 (MQ=255)
                                      cccGCGTGTTGCTTGAGCGCCAGCAAACTATTGGCGCATTGCAGGATTTCACCGCCGGGCTACAGCCGGTACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGCCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGt                                                                                                               <  2:728600/149‑1 (MQ=255)
                                      cccGCGTGTTGCTTGAGCGCCAGCAAACTATTGGCGCATTGCAGGATTTCACCGCCGGGCTACAGCCGGTACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGCCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTAt                                                                                                             <  2:875713/151‑1 (MQ=255)
                                        cgcgTGTTGCTTGAGCGCCAGCAAACTATTGGCGCATTGCAGGATTTCACCGCCGGGCTACAGCCGGTACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATgc                                                                                                           >  2:186301/1‑151 (MQ=255)
                                        cgcgTGTTGCTTGAGCGCCAGCAAACTATTGGCGCATTGCAGGATTTCACCGCCGGGCTACAGCCGGTACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGCCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATgc                                                                                                           <  1:68916/151‑1 (MQ=255)
                                        cgcgTGTTGCTTGAGCGCCAGCAAACTATTGGCGCATTGCAGGATTTCACCGCCGGGCTACAGCCGGTACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGCCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATg                                                                                                            >  2:712928/1‑150 (MQ=255)
                                          cgTGTTGCTTGAGCGCCAGCAAACTATTGGCGCATTGCAGGATTTCACCGCCGGGCTACAGCCGGTACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGCCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATgcgc                                                                                                         <  2:1455409/151‑1 (MQ=255)
                                            tgtTGCTTGAGCGCCAGCAAACTATTGGCGCATTGCAGGATTTCACCGCCGGGCTACAGCCGGTACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATGCGcc                                                                                                        >  2:813075/1‑150 (MQ=255)
                                                gCTTGAGCGCCAGCAAACTATTGGCGCATTGCAGGATTTCACCGCCGGGCTACAGCCGGTACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGCCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCt                                                                                                   <  1:173898/151‑1 (MQ=255)
                                                    gAGCGCCAGCAAACTATTGGCGCATTGCAGGATTTCACCGCCGGGCTACAGCCGGTACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGCCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTcc                                                                                               >  1:177966/1‑151 (MQ=255)
                                                    gAGCGCCAGCAAACTATTGGCGCATTGCAGGATTTCACCGCCGGACTACAGCCGGTACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGCCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTcc                                                                                               >  1:413476/1‑151 (MQ=255)
                                                      gcgcCAGCAAACTATTGGCGCATTGCAGGATTTCACCGCCGGGCTACAGCCGGTACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGCCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAg                                                                                             <  1:1079932/151‑1 (MQ=255)
                                                       ccccaccaAACTATTGGCGCATTGCCGGATTTCACCGCCGGGCTACAGCCGGTACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGCCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAg                                                                                             <  1:1568648/148‑1 (MQ=255)
                                                         ccAGCAACCTATTGGCGCATTGCAGGATTTCACCGCCGGGCTACAGCCGGTACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGCCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCa                                                                                              <  1:712870/147‑1 (MQ=255)
                                                          cAGCAAACTATTGGCGCATTGCAGGATTTCACCGCCGGGCTACAGCCGGTACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGCCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAAc                                                                                         <  2:1534199/151‑1 (MQ=255)
                                                          cAGCAAACTATTGGCGCATTGCAGGATTTCACCGCCGGGCTACAGCCGGTACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGCCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAAc                                                                                         >  1:1489348/1‑151 (MQ=255)
                                                                  tATTGGCGCATTGCAGGATTTCACCGCCGGGCTACAGCCGGTACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGCCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCgg                                                                                   >  2:642116/1‑149 (MQ=255)
                                                                  tATTGGCGCATTGCAGGATTTCACCGCCGGGCTACAGCCGGTACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGCCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCgg                                                                                   >  2:1494987/1‑149 (MQ=255)
                                                                  tATTGGCGCATTGCAGGATTTCACCGCCGGGCTACAGCCGGTACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGCCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCgg                                                                                   >  2:1354917/1‑149 (MQ=255)
                                                                   atgggCGCATTGCAGGATTTCACCGCCGGGCTACAGCCGGTACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGCCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAg                                                                                 <  2:1001604/147‑1 (MQ=255)
                                                                     tGGCGCATTGCAGGATTTCACCGCCGGGCTACAGCCGGTACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGCCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCtg                                                                              >  2:942936/1‑151 (MQ=255)
                                                                       gcgcATTGCAGGATTTCACCGCCGGGCTACAGCCGGTACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGCCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCtgcg                                                                            <  1:1647560/151‑1 (MQ=255)
                                                                       gcgcATTGCAGGATTTCACCGCCGGGCTACAGCCGGTACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGCCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCtgcg                                                                            <  2:1286153/151‑1 (MQ=255)
                                                                          cATTGCAGGATTTCACCGCCGGGCTACAGCCGGTACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGCCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCtgcgtgc                                                                         <  2:1370192/151‑1 (MQ=255)
                                                                           aTTGCAGGATTTCACCGCCGGGCTACAGCCGGTACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGCCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCtgcgtgcg                                                                        >  2:1227961/1‑151 (MQ=255)
                                                                           aTTGCAGGATTTCACCGCCGGGCTACAGCCGGTACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGCCTGGCTTTACGAACTGCTCACCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCtgcgtg                                                                          >  2:324774/1‑149 (MQ=255)
                                                                              gCAGGATTTCACCGCCGGGCTACAGCCGGTACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGCCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTgcgc                                                                       >  2:1577347/1‑149 (MQ=255)
                                                                                 ggATTTCACCGCCGGGCTACAGCCGGTACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGCCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGtt                                                                   <  1:106996/150‑1 (MQ=255)
                                                                                 ggATTTCACCGCCGGGCTACAGCCGGTACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGCCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGtt                                                                   <  1:1056625/150‑1 (MQ=255)
                                                                                  gATTTCACCGCCGGGCTACAGCCGGTACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGCCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAg                                                                 <  2:256500/151‑1 (MQ=255)
                                                                                   aTTTCACCGCCGGGCTACAGCCGGTACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGCCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGtt                                                                   >  2:923713/1‑148 (MQ=255)
                                                                                     ttCACCGCCGGGCTACAGCCGGTACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGCCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGaa                                                               >  1:57276/1‑150 (MQ=255)
                                                                                      tCACCGCCGGGCTACAGCCGGTACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGCCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGaaa                                                              <  1:1566106/150‑1 (MQ=255)
                                                                                         ccgccgGGCTACAGCCGGTACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGCCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAAc                                                             <  1:147106/148‑1 (MQ=255)
                                                                                         ccgccgGGCTACAGCCGGTACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGCCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGt                                                          <  1:989424/151‑1 (MQ=255)
                                                                                         ccgccgGGCTACAGCCGGTACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGCCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGt                                                          <  2:223428/151‑1 (MQ=255)
                                                                                           gccgGGCTACAGCCGGTACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGCCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCg                                                        <  2:1191677/151‑1 (MQ=255)
                                                                                           gccgGGCTACAGCCGGTACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGCCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCg                                                        >  1:282836/1‑151 (MQ=255)
                                                                                            ccgGGCTACAGCCGGTACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGCCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCg                                                        >  2:887747/1‑150 (MQ=255)
                                                                                              gggCTACAGCCGGTACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGCCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATa                                                     <  1:575304/151‑1 (MQ=255)
                                                                                                  tACAGCCGGTACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGCCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGc                                                 >  2:717915/1‑151 (MQ=255)
                                                                                                   aCAGCCGGTACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGCCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGc                                                 >  2:954639/1‑150 (MQ=255)
                                                                                                    cAGCCGGTACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGCCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCAc                                               <  2:543611/151‑1 (MQ=255)
                                                                                                    cAGCCGGTACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGCCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCAc                                               <  1:1383313/151‑1 (MQ=255)
                                                                                                     aGCCGGTACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGCCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCAcc                                              >  1:386375/1‑151 (MQ=255)
                                                                                                      gCCGGTACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGCCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCg                                             >  1:1442553/1‑151 (MQ=255)
                                                                                                      gCCGGTACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGCCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCg                                             <  2:413508/151‑1 (MQ=255)
                                                                                                        cGGTACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGCCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGt                                           <  2:741063/151‑1 (MQ=255)
                                                                                                        cGGTACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGCCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGt                                           <  2:426443/151‑1 (MQ=255)
                                                                                                           tACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGCCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTAc                                         >  1:543013/1‑150 (MQ=255)
                                                                                                            aCTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGCCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTAc                                         >  2:837839/1‑149 (MQ=255)
                                                                                                            aCTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGCCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTAc                                         >  2:774574/1‑149 (MQ=255)
                                                                                                               gCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGCCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAgg                                      <  1:1166798/149‑1 (MQ=255)
                                                                                                               gCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGCCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGgcg                                    <  2:1446601/151‑1 (MQ=255)
                                                                                                               gCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGCCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGgcg                                    <  2:1694234/151‑1 (MQ=255)
                                                                                                               gCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGCCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGgcg                                    >  2:898679/1‑151 (MQ=255)
                                                                                                                     ggTCGGCGACCTGGAACGTATTCTGGCACGCCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGt                              <  1:750440/151‑1 (MQ=255)
                                                                                                                         ggCGACCTGGAACGTATTCTGGCACGCCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTgaga                          <  2:1015501/151‑1 (MQ=255)
                                                                                                                         ggCGACCTGGAACGTATTCTGGCACGCCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTgaga                          <  2:1331649/151‑1 (MQ=255)
                                                                                                                          gCGACCTGGAACGTATTCTGGCACGCCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTgaga                          >  2:1672085/1‑150 (MQ=255)
                                                                                                                           cGACCTGGAACGTATTCTGGCACGCCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTgaga                          >  2:1304029/1‑149 (MQ=255)
                                                                                                                           cGACCTGGAACGTATTCTGGCACGCCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTgaga                          >  2:609323/1‑149 (MQ=255)
                                                                                                                            gACCTGGAACGTATTCTGGCACGCCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTgaga                          >  2:412634/1‑148 (MQ=255)
                                                                                                                             aCCTGGAACGTATTCTGGCACGCCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGAGAAGAt                      <  2:282658/151‑1 (MQ=255)
                                                                                                                               cTGGAACGTATTCTGGCACGCCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGAGAAGATgg                    >  2:1365701/1‑151 (MQ=255)
                                                                                                                                 ggAACGTATTCTGGCACGCCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGAGAAGATggg                   >  2:1371654/1‑150 (MQ=255)
                                                                                                                                 ggAACGTATTCTGGCACGCCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGc                  <  2:756327/151‑1 (MQ=255)
                                                                                                                                   aaCGTATTCTGGCACGCCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCg                 >  2:852140/1‑150 (MQ=255)
                                                                                                                                   aaCGTATTCTGGCACGCCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCg                 >  1:1231500/1‑150 (MQ=255)
                                                                                                                                    aCGTATTCTGGCACGCCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCGAg               <  2:759582/151‑1 (MQ=255)
                                                                                                                                       tATTCTGGCACGCCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCGAGttt            >  2:771673/1‑151 (MQ=255)
                                                                                                                                       tATTCTGGCACGCCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCGAGttt            >  2:615622/1‑151 (MQ=255)
                                                                                                                                        aTTCTGGCACGCCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCGAGTTTg           >  2:751560/1‑151 (MQ=255)
                                                                                                                                         ttCTGGCACGTCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCGAGTTTGc          >  2:1672667/1‑151 (MQ=255)
                                                                                                                                          tCTGGCACGCCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCGAGTTTGc          <  1:1695387/150‑1 (MQ=255)
                                                                                                                                           cTGGCACGCCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCg        >  2:1501669/1‑151 (MQ=255)
                                                                                                                                             ggCACGCCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCg        <  1:1169109/149‑1 (MQ=255)
                                                                                                                                               cACGCCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGc     <  1:837777/150‑1 (MQ=255)
                                                                                                                                               cACGCCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCt    <  2:27949/151‑1 (MQ=255)
                                                                                                                                               cACGCCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCt    >  1:1653644/1‑151 (MQ=255)
                                                                                                                                                 cGTCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTgc  <  1:186287/151‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TATGCTGAAACGCTGGCTGCATATGCCAGTGCGCGATACCCGCGTGTTGCTTGAGCGCCAGCAAACTATTGGCGCATTGCAGGATTTCACCGCCGGGCTACAGCCGGTACTGCGTCAGGTCGGCGACCTGGAACGTATTCTGGCACGTCTGGCTTTACGAACTGCTCGCCCACGCGATCTGGCCCGTATGCGCCACGCTTTCCAGCAACTGCCGGAGCTGCGTGCGCAGTTAGAAACTGTCGATAGTGCACCGGTACAGGCGCTACGTGAGAAGATGGGCGAGTTTGCCGAGCTGC  >  NC_000913/2858007‑2858302

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: