Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,901,816	T→C	F409L (TTC→CTC)	yqcE →	putative MFS transporter, inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,901,816	0	T	C	100.0%	373.1 / NA	94	F409L (TTC→CTC)	yqcE	putative MFS transporter, inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (43/51);  total (43/51)

CGGTGATTGGATTCCTGCCGGATGTCTTCGTTTACCCAATTATCGGCCACTGGCAAGACACCCTGCCCGCTGCAGAAGCCTACCGCAATATGTGGCTGATGGGCATGGCGGCGCTTGGCATGGTGATTGTCTTTACCTTTTTGCTGTTCCAAAAAATTCGTACTGCTGATAGCGCCCCCGCAATGGCTAGCAGCAAGTAAGCCAGGAAAATTACAGGAGAAAAATCATGTCGAAAAAATACATCATAGGGATTGATGGCGGAAGTCAGAGCACAAAAGTGGTGATGTACGAT  >  NC_000913/2901670‑2901961                                                                                                                                                   |                                                                                                                                                  cGGTGATTGGATTCCTGCCGGATGTCTTCGTTTACCCAATTATCGGCCACTGGCAAGACACCCTGCCCGCTGCAGAAGCCTACCGCAATATGTGGCTGATGGGCATGGCGGCGCTTGGCATGGTGATTGTCTTTACCTTTTTGCTGCTcc                                                                                                                                                <  2:967614/150‑1 (MQ=255)  ggTGATTGGATTCCTGCCGGATGTCTTCGTTTACCCAATTATCGGCCACTGGCAAGACACCCTGCCCGCTGCAGAAGCCTACCGCAATATGTGGCTGATGGGCATGGCGGCGCTTGGCATGGTGATTGTCTTTACCTTTTTGCTGCTCCaa                                                                                                                                              <  1:1677664/151‑1 (MQ=255)   gTGATTGGATTCCTGCCGGATGTCTTCGTTTACCCAATTATCGGCCACTGGCAAGACACCCTGCCCGCTGCAGAAGCCTACCGCAATATGTGGCTGATGGGCATGGCGGCGCTTGGCATGGTGATTGTCTTTACCTTTTTGCTGCTCCaaa                                                                                                                                             <  1:151211/151‑1 (MQ=255)     gATTGGATTCCTGCCGGATGTCTTCGTTTACCCAATTATCGGCCACTGGCAAGACACCCTGCCCGCTGCAGAAGCCTACCGCAATATGTGGCTGATGGGCATGGCGGCGCTTGGCATGGTGATTGTCTTTACCTTTTTGCTGCTCCaaaaa                                                                                                                                           <  2:355245/151‑1 (MQ=255)        tGGATTCCTGCCGGATGTCTTCGTTTACCCAATTATCGGCCACTGGCAAGACACCCTGCCCGCTGCAGAAGCCTACCGCAATATGTGGCTGATGGGCATGGCGGCGCTTGGCATGGTGATTGTCTTTACCTTTTTGCTGCTCCAAAAAAtt                                                                                                                                        >  2:1416287/1‑151 (MQ=255)        tGGATTCCTGCCGGATGTCTTCGTTTACCCAATTATCGGCCACTGGCAAGACACCCTGCCCGCTGCAGAAGCCTACCGCAATATGTGGCTGATGGGCATGGCGGCGCTTGGCATGGTGATTGTCTTTACCTTTTTGCTGCTCCAAAAAAt                                                                                                                                         <  1:996564/150‑1 (MQ=255)           aTTCCTGCCGGATGTCTTCGTTTACCGAATTATCGGCCACTGGCAAGACACCCTGCCCGCTGCAGAAGCCTACCGCAATATGTGGCTGATGGGCATGGCGGCGCTTGGCATGGTGATTGTCTTTACCTTTTTGCTGCTCCAAAAAATTCGt                                                                                                                                     >  1:724145/1‑151 (MQ=255)            ttCCTGCCGGATGTCTTCGTTTACCCAATTATCGGCCACTGGCAAGACACCCTGCCCGCTGCAGAAGCCTACCGCAATATGTGGCTGATGGGCATGGCGGCGCTTGGCATGGTGATTGTCTTTACCTTTTTGCTGCTCCAAAAAATTCGt                                                                                                                                     <  1:639274/150‑1 (MQ=255)            ttCCTGCCGGATGTCTTCGTTTACCCAATTATCGGCCACTGGCAAGACACCCTGCCCGCTGCAGAAGCCTACCGCAATATGTGGCTGATGGGCATGGCGGCGCTTGGCATGGTGATTGTCTTTACCTTTTTGCTGCTCCAAAAAATTCGt                                                                                                                                     >  2:1591122/1‑150 (MQ=255)            ttCCTGCCGGATGTCTTCGTTTACCCAATTATCGGCCACTGGCAAGACACCCTGCCCGCTGCAGAAGCCTACCGCAATATGTGGCTGATGGGCATGGCGGCGCTTGGCATGGTGATTGTCTTTACCTTTTTGCTGCTCCAAAAAATTCGt                                                                                                                                     >  1:1246445/1‑150 (MQ=255)              ccTGCCGGATGTCTTCGTTTACCCAATTATCGGCCACTGGCAAGACACCCTGCCCGCTGCAGAAGCCTACCGCAATATGTGGCTGATGGGCATGGCGGCGCTTGGCATGGTGATTGTCTTTACCTTTTTGCTGCTCCAAAAAATTCGTAc                                                                                                                                   <  2:613526/150‑1 (MQ=255)               cTGCCGGATGTCTTCGTTTACCCAATTATCGGCCACTGGCAAGACACCCTGCCCGCTGCAGAAGCCTACCGCAATATGTGGCTGATGGGCATGGCGGCGCTTGGCATGGTGATTGTCTTTACCTTTTTGCTGCTCCAAAAAATTCGTActg                                                                                                                                 >  1:78802/1‑151 (MQ=255)               cTGCCGGATGTCTTCGTTTACCCAATTATCGGCCACTGGCAAGACACCCTGCCCGCTGCAGAAGCCTACCGCAATATGTGGCTGATGGGCATGGCGGCGCTTGGCATGGTGATTGTCTTTACCTTTTTGCTGCTCCAAAAAATTCGTAct                                                                                                                                  <  2:960621/150‑1 (MQ=255)                 gCCGGATGTCTTCGTTTACCCAATTATCGGCCACTGGCAAGACACCCTGCCCGCTGCAGAAGCCTACCGCAATATGTGGCTGATGGGCATGGCGGCGCTTGGCATGGTGATTGTCTTTACCTTTTTGCTGCTCCAAAAAATTCGTActgct                                                                                                                               <  2:1133534/151‑1 (MQ=255)                   cGGATGTCTTCGTTTACCCAATTATCGGCCACTGGCAAGACACCCTGCCCGCTGCAGAAGCCTACCGCAATATGTGGCTGATGGGCATGGCGGCGCTTGGCATGGTGATTGTCTTTACCTTTTTGCTGCTCCAAAAAATTCGTACTGCTGa                                                                                                                             <  2:403551/151‑1 (MQ=255)                   cGGATGTCTTCGTTTACCCAATTATCGACCACTGGCAAGACACCCTGCCCGCTGCAGAAGCCTACCGCAATATGTGGCTGATGGGCATGGCGGCGCTTGGCATGGGGATTGTCTTTACCTTTTTGCTGCTCCAAAAAATTCGTActgctg                                                                                                                              >  2:182370/1‑150 (MQ=255)                    ggATGTCTTCGTTTACCCAATTATCGGCCACTGGCAAGACACCCTGCCCGCTGCAGAAGCCTACCGCAATATGTGGCTGATGGGCATGGCGGCGCTTGGCATGGTGATTGTCTTTACCTTTTTGCTGCTCCAAAAAATTCGTACTGCTGAt                                                                                                                            >  2:868564/1‑151 (MQ=255)                      aTGTCTTCGTTTACCCAATTATCGGCCACTGGCAAGACACCCTGCCCGCTGCAGAAGCCTACCGCAATATGTGGCTGATGGGCATGGCGGCGCTTGGCATGGTGATTGTCTTTACCTTTTTGCTGCTCCAAAAAATTCGTACTGCTGATAg                                                                                                                          <  2:346598/151‑1 (MQ=255)                       tGTCTTCGTTTACCCAATTATCGGCCACTGGCAAGACACCCTGCCCGCTGCAGAAGCCTACCGCAATATGTGGCTGATGGGCATGGCGGCGCTTGGCATGGTGATTGTCTTTACCTTTTTGCTGCTCCAAAAAATTCGTACTGCTGATAgc                                                                                                                         >  2:279967/1‑151 (MQ=255)                             cGTTTACCCAATTATCGGCCACTGGCAAGACACCCTGCCCGCTGCAGAAGCCTACCGCAATATGTGGCTGATGGGCATGGCGGCGCTTGGCATGGTGATTGTCTTTACCTTTTTGCTGCTCCAAAAAATTCGTACTGCTGATAGCGccccc                                                                                                                   >  2:123300/1‑151 (MQ=255)                             cGTTTACCCAATTATCGGCCACTGGCAAGACACCCTGCCCGCTGCAGAAGCCTACCGCAATATGTGGCTGATGGGCATGGCGGCGCTTGGCATGGTGATTGTCTTTACCTTTTTGCTGCTCCAAAAAATTCGTACTGCTGATAGCGccccc                                                                                                                   <  1:1248692/151‑1 (MQ=255)                                ttACCCAATTATCGGCCACTGGCAAGACACCCTGCCCGCTGCAGAAGCCTACCGCAATATGTGGCTGATGGGCATGGCGGCGCTTGGCATGGTGATTGTCTTTACCTTTTTGCTGCTCCAAAAAATTCGTACTGCTGATAGCGCCCCCGc                                                                                                                 >  2:1011927/1‑150 (MQ=255)                                 tACCCAATTATCGGCCACTGGCAAGACACCCTGCCCGCTGCAGAAGCCTACCGCAATATGTGGCTGATGGGCATGGCGGCGCTTGGCATGGTGATTGTCTTTACCTTTTTGCTGCTCCAAAAAATTCGTACTGCTGATAGCGCCCCCGCa                                                                                                                >  1:991971/1‑150 (MQ=255)                                     cAATTATCGGCCACTGGCAAGACACCCTGCCCGCTGCAGAAGCCTACCGCAATATGTGGCTGATGGGCATGGCGGCGCTTGGCATGGTGATTGTCTTTACCTTTTTGCTGCTCCAAAAAATTCGTACTGCTGATAGCGCCCCCGCAATGGc                                                                                                           >  2:1194953/1‑151 (MQ=255)                                      aaTTATCGGCCACTGGCAAGACACCCTGCCCGCTGCAGAAGCCTACCGCAATATGTGGCTGATGGGCATGGCGGCGCTTGGCATGGTGATTGTCTTTACCTTTTTGCTGCTCCAAAAAATTCGTACTGCTGATAGCGCCCCCGCAATGGc                                                                                                           >  1:1080049/1‑150 (MQ=255)                                        ttATCGGCCACTGGCAAGACACCCTGCCCGCTGCAGAAGCCTACCGCAATATGTGGCTGATGAGCATGGCGGCGCTTGGCATGGTGATTGTCTTTACCTTTTTGCTGCTCCAAAAAATTCGTACTGCTGATAGCGCCCCCGCAATGGCt                                                                                                          >  2:1282025/1‑149 (MQ=255)                                         tATCGGCCACTGGCAAGACACCCTGCCCGCTGCAGAAGCCTACCGCAATATGTGGCTGATGGGCATGGCGGCGCTTGGCATGGTGATTGTCTTTACCTTTTTGCTGCTCCAAAAAATTCGTACTGCTGATAGCGCCCCCGCAATGGCTag                                                                                                        <  2:962946/150‑1 (MQ=255)                                           tCGGCCACTGGCAAGACACCCTGCCCGCTGCAGAAGCCTACCGCAATATGTGGCTGATGGGCATGGCGGCGCTTGGCATGGTGATTGTCTTTACCTTTTTGCTGCTCCAAAAAATTCGTACTGCTGATAGCGCCCCCGCAATGGCTagc                                                                                                       >  1:639316/1‑149 (MQ=255)                                           tCGGCCACTGGCAAGACACCCTGCCCGCTGCAGAAGCCTACCGCAATATGTGGCTGATGGGCATGGCGGCGCTTGGCATGGTGATTGTCTTTACCTTTTTGCTGCTCCAAAAAATTCGTACTGCTGATAGCGCCCCCGCAATGGCTagc                                                                                                       >  2:627106/1‑149 (MQ=255)                                              gCCACTGGCAAGACACCCTGCCCGCTGCAGAAGCCTACCGCAATATGTGGCTGATGGGCATGGCGGCGCTTGGCATGGTGATTGTCTTTACCTTTTTGCTGCTCCAAAAAATTCGTACTGCTGATAGCGCCCCCGCAATGGCTAGCAGCaa                                                                                                  <  1:434674/151‑1 (MQ=255)                                                cACTGGCAAGACACCCTGCCCGCTGCAGAAGCCTACCGCAATATGTGGCTGATGGGCATGGCGGCGCTTGGCATGGTGATTGTCTTTACCTTTTTGCTGCTCCAAAAAATTCGTACTGCTGATAGCGCCCCCGCAATGGCTAGCAGCAAGt                                                                                                >  2:35197/1‑151 (MQ=255)                                                 aCTGGCAAGACACCCTGCCCGCTGCAGAAGCCTACCGCAATATGTGGCTGATGGGCATGGCGGCGCTTGGCATGGTGATTGTCTTTACCTTTTTGCTGCTCCAAAAAATTCGTACTGCTGATAGCGCCCCCGCAATGGCTAGCAGCAAGt                                                                                                >  1:592706/1‑150 (MQ=255)                                                    ggCAAGACACCCTGCCCGCTGCAGAAGCCTACCGCAATATGTGGCTGATGGGCATGGCGGCGCTTGGCATGGTGATTGTCTTTACCTTTTTGCTGCTCCAAAAAATTCGTACTGCTGATAGCGCCCCCGCAATGGCTAGCAGCAAGTaa                                                                                              <  2:949693/149‑1 (MQ=255)                                                    ggCAAGACACCCTGCCCGCTGCAGAAGCCTACCGCAATATGTGGCTGATGGGCATGGCGGCGCTTGGCATGGTGATTGTCTTTACCTTTTTGCTGCTCCAAAAAATTCGTACTGCTGATAGCGCCCCCGCAATGGCTAGCAGCAAGTAAg                                                                                             <  1:1497569/150‑1 (MQ=255)                                                    ggCAAGACACCCTGCCCGCTGCAGAAGCCTACCGCAATATGTGGCTGATGGGCATGGCGGCGCTTGGCATGGTGATTGTCTTTACCTTTTTGCTGCTCCAAAAAATTCGTACTGCTGATAGCGCCCCCGCAATGGCTAGCAGCAAGTAAg                                                                                             <  2:1548357/150‑1 (MQ=255)                                                        aGACACCCTGCCCGCTGCAGAAGCCTACCGCAATATGTGGCTGATGGGCATGGCGGCGCTTGGCATGGTGATTGTCTTTACCTTTTTGCTGCTCCAAAAAATTCGTACTGCTGATAGCGCCCCCGCAATGGCTAGCAGCAAGTAAGCCAgg                                                                                        <  2:1029254/151‑1 (MQ=255)                                                           cacCCTGCCCGCTGCAGAAGCCTACCGCAATATGTGGCTGATGGGCATGGCGGCGCTTGGCATGGTGATTGTCTTTACCTTTTTGCTGCTCCAAAAAATTCGTACTGCTGATAGCGCCCCCGCAATGGCTAGCAGCAAGTAAGCCAGGaaa                                                                                     <  2:1464256/151‑1 (MQ=255)                                                            acCCTGCCCGCTGCAGAAGCCTACCGCAATATGTGGCTGATGGGCATGGCGGCGCTTGGCATGGTGATTGTCTTTACCTTTTTGCTGCTCCAAAAAATTCGTACTGCTGATAGCGCCCCCGCAATGGCTAGCAGCAAGTAAGCCAGGaaa                                                                                     >  1:1168588/1‑150 (MQ=255)                                                             cccTGCCCGCTGCAGAAGCCTACCGCAATATGTGGCTGATGGGCATGGCGGCGCTTGGCATGGTGATTGTCTTTACCTTTTTGCTGCTCCAAAAAATTCGTACTGCTGATAGCGCCCCCGCAATGGCTAGCAGCAAGTAAGCCAGGaaaa                                                                                    <  1:620297/150‑1 (MQ=255)                                                             cccTGCCCGCTGCAGAAGCCTACCGCAATATGTGGCTGATGGGCATGGCGGCGCTTGGCATGGTGATTGTCTTTACCTTTTTGCTGCTCCAAAAAATTCGTACTGCTGATAGCGCCCCCGCAATGGCTAGCAGCAAGTAAGCCAGGAAAAt                                                                                   <  1:121252/151‑1 (MQ=255)                                                              ccTGCCCGCTGCAGAAGCCTACCGCAATATGTGGCTGATGGGCATGGCGGCGCTTGGCATGGTGATTGTCTTTACCTTTTTGCTGCTCCAAAAAATTCGTACTGCTGATAGCGCCCCCGCAATGGCTAGCAGCAAGTAAGCCAGGAAAAtt                                                                                  <  2:303918/151‑1 (MQ=255)                                                              ccTGCCCGCTGCAGAAGCCTACCGCAATATGTGGCTGATGGGCATGGCGGCGCTTGGCATGGTGATTGTCTTTACCTTTTTGCTGCTCCAAAAAATTCGTACTGCTGATAGCGCCCCCGCAATGGCTAGCAGCAAGTAAGCCAGGAAAAtt                                                                                  <  1:36953/151‑1 (MQ=255)                                                               cTGCCCGCTGCAGAAGCCTACCGCAATATGTGGCTGATGGGCATGGCGGCGCTTGGCATGGAGATTGTCTTTACCTTTTTGCTGCTCCAAAAAATTCGTACTGCTGATAGCGCCCCCGCAATGGCTAGCAGCAAGTAAGCCAGGAAAAtt                                                                                  >  1:613864/1‑150 (MQ=255)                                                                 gCCCGCTGCAGAAGCCTACCGCAATATGTGGCTGATGGGCATGGCGGCGCTTGGCATGGTGATTGTCTTTACCTTTTTGCTGCTCCAAAAAATTCGTACTGCTGATAGCGCCCCCGCAATGGCTAGCAGCAAGTAAGCCAGGAAAATTACa                                                                               <  1:568596/151‑1 (MQ=255)                                                                    cGCTGCAGAAGCCTACCGCAATATGTGGCTGATGGGCATGGCGGCGCTTGGCATGGTGATTGTCTTTACCTTTTTGCTGCTCCAAAAAATTCGTACTGCTGATAGCGCCCCCGCAATGGCTAGCAGCAAGTAAGCCAGGAAAATTACAgg                                                                             <  2:1166091/150‑1 (MQ=255)                                                                    cGCTGCAGAAGCCTACCGCAATATGTGGCTGATGGGCATGGCGGCGCTTGGCATGGTGATTGTCTTTACCTTTTTGCTGCTCCAAAAAATTCGTACTGCTGATAGCGCCCCCGCAATGGCTAGCAGCAAGTAAGCCAGGAAAATTACAgg                                                                             >  1:823460/1‑150 (MQ=255)                                                                     gCTGCAGAAGCCTACCGCAATATGTGGCTGATGGGCATGGCGGCGCTTGGCATGGTGATTGTCTTTACCTTTTTGCTGCTCCAAAAAATTCGTACTGCTGATAGCGCCCCCGCAATGGCTAGCAGCAAGTAAGCCAGGAAAATTACAgg                                                                             >  2:228476/1‑149 (MQ=255)                                                                       tGCAGAAGCCTACCGCAATATGTGGCTGATGGGCATGGCGGCGCTTGGCATGGTGATTGTCTTTACCTTTTTGCTGCTCCAAAAAATTCGTACTGCTGATAGCGCCCCCGCAATGGCTAGCAGCAAGTAAGCCAGGAAAATTACAGgaga                                                                          >  1:1523042/1‑150 (MQ=255)                                                                       tGCAGAAGCCTACCGCAATATGTGGCTGATGGGCATGGCGGCGCTTGGCATGGTGATTGTCTTTACCTTTTTGCTGCTCCAAAAAATTCGTACTGCTGATAGCGCCCCCGCAATGGCTAGCAGCAAGTAAGCCAGGAAAATTACAGgaga                                                                          >  2:1659158/1‑150 (MQ=255)                                                                        gCAGAAGCCTACCGCAATATGTGGCTGATGGGCATGGCGGCGCTTGGCATGGTGATTGTCTTTACCTTTTTGCTGCTCCAAAAAATTCGTACTGCTGATAGCGCCCCCGCAATGGCTAGCAGCAAGTAAGCCAGGAAAATTACAGgaga                                                                          <  1:811071/149‑1 (MQ=255)                                                                        gCAGAAGCCTACCGCAATATGTGGCTGATGGGCATGGCGGCGCTTGGCATGGTGATTGTCTTTACCTTTTTGCTGCTCCAAAAAATTCGTACTGCTGATAGCGCCCCCGCAATGGCTAGCAGCAAGTAAGCCAGGAAAATTACAGGAGaa                                                                         >  1:856680/1‑150 (MQ=255)                                                                           gAAGCCTACCGCAATATGTGGCTGATGGGCATGGCGGCGCTTGGCATGGTGATTGTCTTTACCTTTTTGCTGCTCCAAAAAATTCGTACTGCTGATAGCGCCCCCGCAATGGCTAGCAGCAAGTAAGCCAGGAAAATTACAGGAGAAAAAt                                                                     >  1:981327/1‑151 (MQ=255)                                                                              gCCTACCGCAATATGTGGCTGATGGGCATGGCGGCGCTTGGCATGGTGATTGTCTTTACCTTTTTGCTGCTCCAAAAAATTCGTACTGCTGATAGCGCCCCCGCAATGGCTAGCAGCAAGTAAGCCAGGAAAATTACAGGAGAAAAATCAt                                                                  <  1:1414477/151‑1 (MQ=255)                                                                              gCCTACCGCAATATGTGGCTGATGGGCATGGCGGCGCTTGGCATGGTGATTGTCTTTACCTTTTTGCTGCTCCAAAAAATTCGTACTGCTGATAGCGCCCCCGCAATGGCTAGCAGCAAGTAAGCCAGGAAAATTACAGGAGAAAAATCAt                                                                  <  2:1069958/151‑1 (MQ=255)                                                                              gCCTACCGCAATATGTGGCTGATGAGCATGGCGGCGCTTGGCATGGTGATTGTCTTTACCTTTTTGCTGCTCCAAAAAATTCGTACTGCTGATAGCGCCCCCGCAATGGCTAGCAGCAAGTAAGCCAGGAAAATTACAGGAGAAAAATCAt                                                                  <  1:1281932/151‑1 (MQ=255)                                                                               ccTACCGCAATATGTGGCTGATGGGCATGGCGGCGCTTGGCATGGTGATTGTCTTTACCTTTTTGCTGCTCCAAAAAATTCGTACTGCTGATAGCGCCCCCGCAATGGCTAGCAGCAAGTAAGCCAGGAAAATTACAGGAGAAAAATCATg                                                                 <  1:367316/151‑1 (MQ=255)                                                                                  aCCGCAATATGTGGCTGATGGGCATGGCGGCGCTTGGCATGGTGATTGTCTTTACCTTTTTGCTGCTCCAAAAAATTCGTACTGCTGATAGCGCCCCCGCAATGGCTAGCAGCAAGTAAGCCAGGAAAATTACAGGAGAAAAATCATGt                                                                <  1:5248/149‑1 (MQ=255)                                                                                   ccGCAATATGTGGCTGATGGGCATGGCGGCGCTTGGCATGGTGATTGTCTTTACCTTTTTGCTGCTCCAAAAAATTCGTACTGCTGATAGCGCCCCCGCAATGGCTAGCAGCAAGTAAGCCAGGAAAATTACAGGAGAAAAATCATGTCGa                                                             <  2:1309890/151‑1 (MQ=255)                                                                                   ccGCAATATGTGGCTGATGGGCATGGCGGCGCTTGGCATGGTGATTGTCTTTACCTTTTTGCTGCTCCAAAAAATTCGTACTGCTGATAGCGCCCCCGCAATGGCTAGCAGCAAGTAAGCCAGGAAAATTACAGGAGAAAAATCATGTCGa                                                             <  2:1506068/151‑1 (MQ=255)                                                                                    cGCAATATGTGGCTGATGGGCATGGCGGCGCTTGGCATGGTGATTGTCTTTACCTTTTTGCTGCTCCAAAAAATTCGTACTGCTGATAGCGCCCCCGCAATGGCTAGCAGCAAGTAAGCCAGGAAAATTACAGGAGAAAAATCATGTCGaa                                                            <  2:717974/151‑1 (MQ=255)                                                                                    cGCAATATGTGGCTGATGGGCATGGCGGCGCTTGGCATGGTGATTGTCTTTACCTTTTTGCTGCTCCAAAAAATTCGTACTGCTGATAGCGCCCCCGCAATGGCTAGCAGCAAGTAAGCCAGGAAAATTACAGGAGAAAAATCATGTCGa                                                             >  2:1295737/1‑150 (MQ=255)                                                                                      cAATATGTGGCTGATGGGCATGGCGGCGCTTGGCATGGTGATTGTCTTTACCTTTTTGCTGCTCCAAAAAATTCGTACTGCTGATAGCGCCCCCGCAATGGCTAGCAGCAAGTAAGCCAGGAAAATTACAGGAGAAAAATCATGTCGaaaa                                                          <  2:1181650/151‑1 (MQ=255)                                                                                      cAATATGTGGCTGATGGGCATGGCGGCGCTTGGCATGGTGATTGTCTTTACCTTTTTGCTGCTCCAAAAAATTCGTACTGCTGATAGCGCCCCCGCAATGGCTAGCAGCAAGTAAGCCAGGAAAATTACAGGAGAAAAATCATGTCGaaa                                                           >  2:416960/1‑150 (MQ=255)                                                                                       aaTATGTGGCTGATGGGCATGGCGGCGCTTGGCATGGTGATTGTCTTTACCTTTTTGCTGCTCCAAAAAATTCGTACTGCTGATAGCGCCCCCGCAATGGCTAGCAGCAAGTAAGCCAGGAAAATTACAGGAGAAAAATCATGTCGaaaaa                                                         <  1:1358450/151‑1 (MQ=255)                                                                                            gtgGCTGATGGGCATGGCGGCGCTTGGCATGGTGATTGTCTTTACCTTTTTGCTGCTCCAAAAAATTCGTACTGCTGATAGCGCCCCCGCAATGGCTAGCAGCAAGTAAGCCAGGAAAATTACAGGAGAAAAATCATGTCGAAAAAATAca                                                    <  2:152674/151‑1 (MQ=255)                                                                                            gtgGCTGATGGGCATGGCGGCGCTTGGCATGGTGATTGTCTTTACCTTTTTGCTGCTCCAAAAAATTCGTACTGCTGATAGCGCCCCCGCAATGGCTAGCAGCAAGTAAGCCAGGAAAATTACAGGAGAAAAATCATGTCGAAAAAATAc                                                     >  1:562066/1‑150 (MQ=255)                                                                                            gtgGCTGATGGGCATGGCGGCGCTTGGCATGGTGATTGTCTTTACCTTTTTGCTGCTCCAAAAAATTCGTACTGCTGATAGCGCCCCCGCAATGGCTAGCAGCAAGTAAGCCAGGAAAATTACAGGAGAAAAATCATGTCGAAAAAATAc                                                     <  2:918628/150‑1 (MQ=255)                                                                                              ggCTGATGGGCATGGCGGCGCTTGGCATGGTGATTGTCTTTACCTTTTTGCTGCTCCAAAAAATTCGTACTGCTGATAGCGCCCCCGCAATGGCTAGCAGCAAGTAAGCCAGGAAAATTACAGGAGAAAAATCATGTCGAAAAAATAcatc                                                  <  2:980299/151‑1 (MQ=255)                                                                                                 tGATGGGCATGGCGGCGCTTGGCATGGTGATTGTCTTTACCTTTTTGCTGCTCCAAAAAATTCGTACTGCTGATAGCGCCCCCGCAATGGCTAGCAGCAAGTAAGCCAGGAAAATTACAGGAGAAAAATCATGTCGAAAAAATAcatcat                                                >  2:644941/1‑150 (MQ=255)                                                                                                  gATGGGCATGGCGGCGCTTGGCATGGTGATTGTCTTTACCTTTTTGCTGCTCCAAAAAATTCGTACTGCTGATAGCGCCCCCGCAATGGCTAGCAGCAAGTAAGCCAGGAAAATTACAGGAGAAAAATCATGTCGAAAAAATAcatcat                                                >  1:186262/1‑149 (MQ=255)                                                                                                  gATGGGCATGGCGGCGCTTGGCATGGTGATTGTCTTTACCTTTTTGCTGCTCCAAAAAATTCGTACTGCTGATAGCGCCCCCGCAATGGCTAGCAGCAAGTAAGCCAGGAAAATTACAGGAGAAAAATCATGTCGAAAAAATACATCATAg                                              <  1:1151150/151‑1 (MQ=255)                                                                                                   aTGGGCATGGCGGCGCTTGGCATGGTGATTGTCTTTACCTTTTTGCTGCTCCAAAAAATTCGTACTGCTGATAGCGCCCCCGCAATGGCTAGCAGCAAGTAAGCCAGGAAAATTACAGGAGAAAAATCATGTCGAAAAAATACATCATAgg                                             >  2:653246/1‑151 (MQ=255)                                                                                                     gggCATGGCGGCGCTTGGCATGGTGATTGTCTTTACCTTTTTGCTGCTCCAAAAAATTCGTACTGCTGATAGCGCCCCCGCAATGGCTAGCAGCAAGTAAGCCAGGAAAATTACAGGAGAAAAATCATGTCGAAAAAATACATCATAggg                                            >  1:133471/1‑150 (MQ=255)                                                                                                     gggCATGGCGGCGCTTGGCATGGTGATTGTCTTTACCTTTTTGCTGCTCCAAAAAATTCGTACTGCTGATAGCGCCCCCGCAATGGCTAGCAGCAAGTAAGCCAGGAAAATTACAGGAGAAAAATCATGTCGAAAAAATACATCATAggg                                            <  2:958600/150‑1 (MQ=255)                                                                                                     gggCATGGCGGCGCTTGGCATGGTGATTGTCTTTACCTTTTTGCTGCTCCAAAAAATTCGTACTGCTGATAGCGCCCCCGCAATGGCTAGCAGCAAGTAAGCCAGGAAAATTACAGGAGAAAAATCATGTCGAAAAAATACATCATAggg                                            <  2:92881/150‑1 (MQ=255)                                                                                                     gggCATGGCGGCGCTTGGCATGGTGATTGTCTTTACCTTTTTGCTGCTCCAAAAAATTCGTACTGCTGATAGCGCCCCCGCAATGGCTAGCAGCAAGTAAGCCAGGAAAATTACAGGAGAAAAATCATGTCGAAAAAATACATCATAggg                                            <  2:1001429/150‑1 (MQ=255)                                                                                                      ggCATGGCGGCGCTTGGCATGGTGATTGTCTTTACCTTTTTGCTGCTCCAAAAAATTCGTACTGCTGATAGCGCCCCCGCAATGGCTAGCAGCAAGTAAGCCAGGAAAATTACAGGAGAAAAATCATGTCGAAAAAATACATCATAGGGAt                                          <  1:1054943/151‑1 (MQ=255)                                                                                                      ggCATGGCGGCGCTTGGCATGGTGATTGTCTTTACCTTTTTGCTGCTCCAAAAAATTCGTACTGCTGATAGCGCCCCCGCAATGGCTAGCAGCAAGTAAGCCAGGAAAATTACAGGAGAAAAATCATGTCGAAAAAATACATCATAGGGAt                                          <  1:868497/151‑1 (MQ=255)                                                                                                        cATGGCGGCGCTTGGCATGGTGATTGTCTTTACCTTTTTGCTGCTCCAAAAAATTCGTACTGCTGATAGCGCCCCCGCAATGGCTAGCAGCAAGTAAGCCAGGAAAATTACAGGAGAAAAATCATGTCGAAAAAATACATCATAGGGATTg                                        <  2:724203/151‑1 (MQ=255)                                                                                                                cgcTTGGCATGGTGATTGTCTTTACCTTTTTGCTGCTCCAAAAAATTCGTACTGCTGATAGCGCCCCCGCAATGGCTAGCAGCAAGTAAGCCAGGAAAATTACAGGAGAAAAATCATGTCGAAAAAATACATCATAGGGATTGATGGCgg                                 >  2:761078/1‑150 (MQ=255)                                                                                                                 gcTTGGCATGGTGATTGTCTTTACCTTTTTGCTGCTCCAAAAAATTCGTACTGCTGATAGCGCCCCCGCAATGGCTAGCAGCAAGTAAGCCAGGAAAATTACAGGAGAAAAATCATGTCGAAAAAATACATCATAGGGATTGATGGCGGaa                               <  2:53020/151‑1 (MQ=255)                                                                                                                 gcTTGGCATGGTGATTGTCTTTACCTTTTTGCTGCTCCAAAAAATTCGTACTGCTGATAGCGCCCCCGCAATGGCTAGCAGCAAGTAAGCCAGGAAAATTACAGGAGAAAAATCATGTCGAAAAAATACATCATAGGGATTGATGGCGGa                                >  2:1711389/1‑150 (MQ=255)                                                                                                                  cTTGGCATGGTGATTGTCTTTACCTTTTTGCTGCTCCAAAAAATTCGTACTGCTGATAGCGCCCCCGCAATGGCTAGCAGCAAGTAAGCCAGGAAAATTACAGGAGAAAAATCATGTCGAAAAAATACATCATAGGGATTGATGGCGGa                                >  2:1239610/1‑149 (MQ=255)                                                                                                                     ggCATGGTGATTGTCTTTACCTTTTTGCTGCTCCAAAAAATTCGTACTGCTGATAGCGCCCCCGCAATGGCTAGCAGCAAGTAAGCCAGGAAAATTACAGGAGAAAAATCATGTCGAAAAAATACATCATAGGGATTGATGGCGGAAGTc                            <  1:1471194/150‑1 (MQ=255)                                                                                                                     ggCATGGTGATTGTCTTTACCTTTTTGCTGCTCCAAAAAATTCGTACTGCTGATAGCGCCCCCGCAATGGCTAGCAGCAAGTAAGCCAGGAAAATTACAGGAGAAAAATCATGTCGAAAAAATACATCATAGGGATTGATGGCGGAAGTc                            <  2:1457920/150‑1 (MQ=255)                                                                                                                       cATGGTGATTGTCTTTACCTTTTTGCTGCTCCAAAAAATTCGTACTGCTGATAGCGCCCCCGCAATGGCTAGCAGCAAGTAAGCCAGGAAAATTACAGGAGAAAAATCATGTCGAAAAAATACATCATAGGGATTGATGGCGGAAGTc                            >  2:1688306/1‑148 (MQ=255)                                                                                                                                tGTCTTTACCTTTTTGCTGCTCCAAAAAATTCGTACTGCTGATAGCGCCCCCGCAATGGCTAGCAGCAAGTAAGCCAGGAAAATTACAGGAGAAAAATCATGTCGAAAAAATACATCATAGGGATTGATGGCGGAAGTCAGAGCACaaa                  >  1:1632498/1‑149 (MQ=255)                                                                                                                                     ttACCTTTTTGCTGCTCCAAAAAATTCGTACTGCTGATAGCGCCCCCGCAATGGCTAGCAGCAAGTAAGCCAGGAAAATTACAGGAGAAAAATCATGTCGAAAAAATACATCATAGGGATTGATGGCGGAAGTCAGAGCACAAAAgtggtg           >  2:115063/1‑151 (MQ=255)                                                                                                                                     ttACCTTTTTGCTGCTCCAAAAAATTCGTACTGCTGATAGCGCCCCCGCAATGGCTAGCAGCAAGTAAGCCAGGAAAATTACAGGAGAAAAATCATGTCGAAAAAATACATCATAGGGATTGATGGCGGAAGTCAGAGCACAAAAgtggt            <  1:391699/150‑1 (MQ=255)                                                                                                                                     ttACCTTTTTGCTGCTCCAAAAAATTCGTACTGCTGATAGCGCCCCCGCAATGGCTAGCAGCAAGTAAGCCAGGAAAATTACAGGAGAAAAATCATGTCGAAAAAATACATCATAGGGATTGATGGCGGAAGTCAGAGCACAAAAgtggt            <  2:541943/150‑1 (MQ=255)                                                                                                                                        ccTTTTTGCTGCTCCAAAAAATTCGTACTGCTGATAGCGCCCCCGCAATGGCTAGCAGCAAGTAAGCCAGGAAAATTACAGGAGAAAAATCATGTCGAAAAAATACATCATAGGGATTGATGGCGGAAGTCAGAGCACAAAAGTGGTGATg        >  2:772872/1‑151 (MQ=255)                                                                                                                                         cTTTTTGCTGCTCCAAAAAATTCGTACTGCTGATAGCGCCCCCGCAATGGCTAGCAGCAAGTAAGCCAGGAAAATTACAGGAGAAAAATCATGTCGAAAAAATACATCATAGGGATTGATGGCGGAAGTCAGAGCACAAAAGTGGTGATGt       >  2:376222/1‑151 (MQ=255)                                                                                                                                         cTTTTTGCTGCTCCAAAAAATTCGTACTGCTGATAGCGCCCCCGCAATGGCTAGCAGCAAGTAAGCCAGGAAAATTACAGGAGAAAAATCATGTCGAAAAAATACATCATA‑GGATTGATGGCGGAAGTCAGAGCACAAAAggggtg           >  1:168610/1‑146 (MQ=255)                                                                                                                                           ttttGCTGCTCCAAAAAATTCGTACTGCTGATAGCGCCCCCGCAATGGCTAGCAGCAAGTAAGCCAGGAAAATTACAGGAGAAAAATCATGTCGAAAAAATACATCATAGGGATTGATGGCGGAAGTCAGAGCACAAAAGTGGTGATGTAc     >  2:1111617/1‑151 (MQ=255)                                                                                                                                              tgctgcTCCAAAAAATTCGTACTGCTGATAGCGCCCCCGCAATGGCTAGCAGCAAGTAAGCCAGGAAAATTACAGGAGAAAAATCATGTCGAAAAAATACATCATAGGGATTGATGGCGGAAGTCAGAGCACAAAAGTGGTGATGTa      <  2:1080134/147‑1 (MQ=255)                                                                                                                                               gctgctCCAAAAAATTCGTACTGCTGATAGCGCCCCCGCAATGGCTAGCAGCAAGTAAGCCAGGAAAATTACAGGAGAAAAATCATGTCGAAAAAATACATCATAGGGATTGATGGCGGAAGTCAGAGCACAAAAGTGGTGATGTACGAt  <  1:127813/150‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                  CGGTGATTGGATTCCTGCCGGATGTCTTCGTTTACCCAATTATCGGCCACTGGCAAGACACCCTGCCCGCTGCAGAAGCCTACCGCAATATGTGGCTGATGGGCATGGCGGCGCTTGGCATGGTGATTGTCTTTACCTTTTTGCTGTTCCAAAAAATTCGTACTGCTGATAGCGCCCCCGCAATGGCTAGCAGCAAGTAAGCCAGGAAAATTACAGGAGAAAAATCATGTCGAAAAAATACATCATAGGGATTGATGGCGGAAGTCAGAGCACAAAAGTGGTGATGTACGAT  >  NC_000913/2901670‑2901961

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑