Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,932,540 T→C K158K (AAA→AAG)  fucO ← L‑1,2‑propanediol oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,932,5400TC95.8% 252.2 / 0.6 71K158K (AAA→AAG) fucOL‑1,2‑propanediol oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (1/2);  new base C (35/33);  total (36/35)
Fisher's exact test for biased strand distribution p-value = 6.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

GGTAATATACCCCTCAATAGCATGAGTGAGCGCATCGACACCCGTCGCAGCTTTCAGCGCTGGAGGCATACCATCCATCATGTCAGCGTCAATAAACGCCACCTGCGGGATATCATGCGGATCAACGCAAACAAACTTGCGCCGTTTCTCTTCGTCAGTGATCACGTAGTTAATGGTCACTTCTGCCGCAGTACCTGCTGTGGTAGGAATTGCCAGAATCGGTACACTGGGTTTATTGGTCGGGGAAAGCCCTTCCAGGCTACGCACATCGGCAAACTCCGGGTTGTTGCTGAT  >  NC_000913/2932396‑2932689
                                                                                                                                                |                                                                                                                                                     
ggTAATATACCCCTCAATAGCATGAGTGAGCGCATCGACACCCGTCGCAGCTTTCAGCGCTGGAGGCATACCATCCATCATGTCAGCGTCAATAAACGCCACCTGCGGGATATCATGCGGATCAACGCAAACAAACTTGCGCCGCTtctcg                                                                                                                                                 <  2:799131/151‑2 (MQ=255)
      ataCCCCTCAATAGCATGAGTGAGCGCATCGACACCCGTCGCAGCTTTCAGCGCTGGAGGCATACCATCCATCATGTCAGCGTCAATAAACGCCACCTGCGGGATATCATGCGGATCAACGCAAACAAACTTGCGCCGCTTCTCTTCGTc                                                                                                                                            >  2:15374/1‑150 (MQ=255)
              cAATAGCATGAGTGAGCGCATCGACACCCGTCGCAGCTTTCAGCGCTGGAGGCATACCATCCATCATGTCAGCGTCAATAAACGCCACCTGCGGGATATCATGCGGATCAACGCAAACAAACTTGCGCCGCTTCTCTTCGTCAGTGATc                                                                                                                                     <  1:179280/149‑1 (MQ=255)
              cAATAGCATGAGTGAGCGCATCGACACCCGTCGCAGCTTTCAGCGCTGGAGGCATACCATCCATCATGTCAGCGTCAATAAACGCCACCTGCGGGATATCATGCGGATCAACGCAAACAAACTTGCGCCGCTTCTCTTCGTCAGTGATCAc                                                                                                                                   <  1:1156108/151‑1 (MQ=255)
                 tAGCATGAGTGAGCGCATCGACACCCGTCGCAGCTTTCAGCGCTGGAGGCATACCATCCATCATGTCAGCGTCAATAAACGCCACCTGCGGGATATCATGCGGATCAACGCAAACAAACTTGCGCCGCTTCTCTTCGTCAGTGATCACGt                                                                                                                                 >  1:1167772/1‑150 (MQ=255)
                  aGCATGAGTGAGCGCATCGACACCCGTCGCAGCTTTCAGCGCTGGAGGCATACCATCCATCATGTCAGCGTCAATAAACGCCACCTGCGGGATATCATGCGGATCAACGCAAACAAACTTGCGCCGTTTCTCTTCGTCAGTGATCACGTa                                                                                                                                <  1:878600/150‑1 (MQ=255)
                    cATGAGTGAGCGCATCGACACCCGTCGCAGCTTTCAGCGCTGGAGGCATACCATCCATCATGTCAGCGTCAATAAACGCCACCTGCGGGATATCATGCGGATCAACGCAAACAAACTTGCGCCGCTTCTCTTCGTCAGTGATCACGTAGtt                                                                                                                             >  2:644541/1‑151 (MQ=255)
                       gagtgagCGCATCGACACCCGTCGCAGCTTTCAGCGCTGGAGGCATACCATCCATCATGTCAGCGTCAATAAACGCCACCTGCGGGATATCATGCGGATCAACGCAAACAAACTTGCGCCGCTTCTCTTCGTCAGTGATCACGTAGTTAAt                                                                                                                          <  1:1497580/151‑1 (MQ=255)
                       gagtgagCGCATCGACACCCGTCGCAGCTTTCAGCGCTGGAGGCATACCATCCATCATGTCAGCGTCAATAAACGCCACCTGCGGGATATCATGCGGATCAACGCAAACAAACTTGCGCCGCTTCTCTTCGTCAGTGATCACGTAGTTAAt                                                                                                                          <  2:768404/151‑1 (MQ=255)
                        agtgagCGCATCGACACCCGTCGCAGCTTTCAGCGCTGGAGGCATACCATCCATCATGTCAGCGTCAATAAACGCCACCTGCGGGATATCATGCGGATCAACGCAAACAAACTTGCGCCGCTTCTCTTCGTCAGTGATCACGTAGTTAATg                                                                                                                         <  1:523952/151‑1 (MQ=255)
                            agCGCATCGACACCCGTCGCAGCTTTCAGCGCTGGAGGCATACCATCCATCATGTCAGCGTCAATAAACGCCACCTGCGGGATATCATGCGTATCAACGCAAACAAACTTGCGCCGCTTCTCTTCGTCAGTGATCACGTAGTTAATGGTc                                                                                                                      >  1:155007/1‑150 (MQ=255)
                              cgcATCGACACCCGTCGCAGCTTTCAGCGCTGGAGGCATACCATCCATCATGTCAGCGTCAATAAACGCCACCTGCGGGATATCATGCGGATCAACGCAAACAAACTTGCGCCGCTTCTCTTCGTCAGTGATCACGTAGTTAATGGTCACt                                                                                                                   >  2:1086137/1‑151 (MQ=255)
                                  tCGACACCCGTCGCAGCTTTCAGCGCTGGAGGCATACCATCCATCATGTCAGCGTCAATAAACGCCACCTGCGGGATATCATGCGGATCAACGCAAACAAACTTGCGCCGCTTCTCTTCGTCAGTGATCACGTAGTTAATGGTCACTTCTg                                                                                                               >  2:1138499/1‑151 (MQ=255)
                                   cGACACCCGTCGCAGCTTTCAGCGCTGGAGGCATACCATCCATCATGTCAGCGTCAATAAACGCCACCTGCGGGATATCATGCGGATCAACGCAAACAAACTTGCGCCGCTTCTCTTCGTCAGTGATCACGTAGTTAATGGTCACTTCTg                                                                                                               <  1:963194/150‑1 (MQ=255)
                                   cGACACCCGTCGCAGCTTTCAGCGCTGGAGGCATACCATCCATCATGTCAGCGTCAATAAACGCCACCTGCGGGATATCATGCGGATCAACGCAAACAAACTTGCGCCGCTTCTCTTCGTCAGTGATCACGTAGTTAATGGTCACTTCTg                                                                                                               <  1:756094/150‑1 (MQ=255)
                                         ccGTCGCAGCTTTCAGCGCTGGAGGCATACCATCCATCATGTCAGCGTCAATAAACGCCACCTGCGGGATATCATGCGGATCAACGCAAACAAACTTGCGCCGCTTCTCTTCGTCAGTGATCACGTAGTTAATGGTCACTTCTGCCGCAGt                                                                                                        <  2:1662493/151‑1 (MQ=255)
                                             cGCAGCTTTCAGCGCTGGAGGCATACCATCCATCATGTCAGCGTCAATAAACGCCACCTGCGGGATATCATGCGGATCAACGCAAACAAACTTGCGCCGCTTCTCTTCGTCAGTGATCACGTAGTTAATGGTCACTTCTGCCGCAGTAcc                                                                                                     <  1:1673057/150‑1 (MQ=255)
                                                aGCTTTCAGCGCTGGAGGCATACCATCCATCATGTCAGCGTCAATAAACGCCACCTGCGGGATATCATGCGGATCAACGCAAACAAACTTGCGCCGCTTCTCTTCGTCAGTGATCACGTAGTTAATGGTCACTTCTGCCGCAGTACctgct                                                                                                 >  2:492873/1‑151 (MQ=255)
                                                aGCTTTCAGCGCTGGAGGCATACCATCCATCATGTCAGCGTCAATAAACGCCACCTGCGGGATATCATGCGGATCAACGCAAACAAACTTGCGCCGCTTCTCTTCGTCAGTGATCACGTAGTTAATGGTCACTTCTGCCGCAGTACctgct                                                                                                 >  2:1149698/1‑151 (MQ=255)
                                                   tttCAGCGCTGGAGGCATACCATCCATCATGTCAGCGTCAATAAACGCCACCTGCGGGATATCATGCGGATCAACGCAAACAAACTTGCGCCGCTTCTCTTCGTCAGTGATCACGTAGTTAATGGTCACTTCTGCCGCAGTACCTGCtgtg                                                                                              >  2:189692/1‑151 (MQ=255)
                                                    ttCAGCGCTGGAGGCATACCATCCATCATGTCAGCGTCAATAAACGCCACCTGCGGGATATCATGCGGATCAACGCAAACAAACTTGCGCCGCTTCTCTTCGTCAGTGATCACGTAGTTAATGGTCACTTCTGCCGCAGTACCTGCTGTgg                                                                                             >  2:899472/1‑151 (MQ=255)
                                                     tCAGCGCTGGAGGCATACCATCCATCATGTCAGCGTCAATAAACGCCACCTGCGGGATATCATGCGGATCAACGCAAACAAACTTGCGCCGCTTCTCTTCGTCAGTGATCACGTAGTTAATGGTCACTTCTGCCGCAGTACCTGCTGTgg                                                                                             <  2:1096069/150‑1 (MQ=255)
                                                      cAGCGCTGGAGGCATACCATCCATCATGTCAGCGTCAATAAACGCCACCTGCGGGATATCATGCGGATCAACGCAAACAAACTTGCGCCGCTTCTCTTCGTCAGTGATCACGTAGTTAATGGTCACTTCTGCCGCAGTACCTGCTGTGGt                                                                                            >  1:512545/1‑150 (MQ=255)
                                                         cgcTGGAGGCATACCATCCATCATGTCAGCGTCAATAAACGCCACCTGCGGGATATCATGCGGATCAACGCAAACAAACTTGCGCCGCTTCTCTTCGTCAGTGATCACGTAGTTAATGGTCACTTCTGCCGCAGTACCTGCTGTGGTAGGa                                                                                        <  1:1609492/151‑1 (MQ=255)
                                                          gcTGGAGGCATACCATCCATCATGTCAGCGTCAATAAACGCCACCTGCGGGATATCATGCGGATCAACGCAAACAAACTTGCGCCGCTTCTCTTCGTCAGTGATCACGTAGTTAATGGTCACTTCTGCCGCAGTACCTGCTGTGGTAGGa                                                                                        >  2:852873/1‑150 (MQ=255)
                                                             ggAGGCATACCATCCATCATGTCAGCGTCAATAAACGCCACCTGCGGGATATCATGCGGATCAACGCAAACAAACTTGCGCCGCTTCTCTTCGTCAGTGATCACGTAGTTAATGGTCACTTCTGCCGCAGTACCTGCTGTGGTAGGAAtt                                                                                     <  1:1690230/150‑1 (MQ=255)
                                                              gAGGCATACCATCCATCATGTCAGCGTCAATAAACGCCACCTGCGGGATATCATGCGGATCAACGCAAACAAACTTGCGCCGCTTCTCTTCGTCAGTGATCACGTAGTTAATGGTCACTTCTGCCGCAGTACCTGCTGTGGTAGGAATTGc                                                                                   >  2:561232/1‑151 (MQ=255)
                                                                ggCATACCATCCATCATGTCAGCGTCAATAAACGCCACCTGCGGGATATCATGCGGATCATCGCAAACAAACTTGCGCCGCTTCTCTTCGTCAGTGATCACGTAGTTAATGGTCACTTCTGCCGCAGTACCTGCTGTGGTATGAATTGCCa                                                                                 <  1:426079/151‑1 (MQ=255)
                                                                   aTACCATCCATCATGTCAGCGTCAATAAACGCCACCTGCGGGATATCATGCGGATCAACGCAAACAAACTTGCGCCGCTTCTCTTCGTCAGTGATCACGTAGTTAATGGTCACTTCTGCCGCAGTACCTGCTGTGGTAGGAATTGCCAGa                                                                               >  1:358560/1‑150 (MQ=255)
                                                                     aCCATCCATCATGTCAGCGTCAATAAACGCCACCTGCGGGATATCATGCGGATCAACGCAAACAAACTTGCGCCGCTTCTCTTCGTCAGTGATCACGTAGTTAATGGTCACTTCTGCCGCAGTACCTGCTGTGGTAGGAATTGCCAGAATc                                                                            >  1:1073287/1‑151 (MQ=255)
                                                                      ccatccatCATGTCAGCGTCAATAAACGCCACCTGCGGGATATCATGCGGATCAACGCAAACAAACTTGCGCCGCTTCTCTTCGTCAGTGATCACGTAGTTAATGGTCACTTCTGCCGCAGTACCTGCTGTGGTAGGAATTGCCAGAATCg                                                                           <  2:526955/151‑1 (MQ=255)
                                                                      ccatccatCATGTCAGCGTCAATAAACGCCACCTGCGGGATATCATGCGGATCAACGCAAACAAACTTGCGCCGCTTCTCTTCGTCAGTGATCACGTAGTTAATGGTCACTTCTGCCGCAGTACCTGCTGTGGTAGGAATTGCCAGAATCg                                                                           >  1:180976/1‑151 (MQ=255)
                                                                       catccatcATGTCAGCGTCAATAAACGCCACCTGCGGGATATCATGCGGATCAACGCAAACAAACTTGCGCCGCTTCTCTTCGTCAGTGATCACGTAGTTAATGGTCACTTCTGCCGCAGTACCTGCTGTGGTAGGAATTGCCAGAATCgg                                                                          >  1:1608403/1‑151 (MQ=255)
                                                                        atccatcATGTCAGCGTCAATAAACGCCACCTGCGGGATATCATGCGGATCAACGCAAACAAACTTGCGCCGCTTCTCTTCGTCAGTGATCACGTAGTTAATGGTCACTTCTGCCGCAGTACCTGCTGTGGTAGGAATTGCCAGAATCGGt                                                                         >  2:266865/1‑151 (MQ=255)
                                                                         tccatcATGTCAGCGTCAATAAACGCCACCTGCGGGATATCATGCGGATCAACGCAAACAAACTTGCGCCGCTTCTCTTCGTCAGTGATCACGTAGTTAATGGTCACTTCTGCCGCAGTACCTGCTGTGGTAGGAATTGCCAGAATCGGt                                                                         >  1:904590/1‑150 (MQ=255)
                                                                          ccatcATGTCAGCGTCAATAAACGCCACCTGCGGGATATCATGCGGATCAACGCAAACAAACTTGCGCCGCTTCTCTTCGTCAGTGATCACGTAGTTAATGGTCACTTCTGCCGCAGTACCTGCTGTGGTAGGAATTGCCAGAATCGGTa                                                                        <  1:142047/150‑1 (MQ=255)
                                                                           catcatGTCAGCGTCAATAAACGCCACCTGCGGGATATCATGCGGATCAACGCAAACAAACTTGCGCCGCTTCTCTTCGTCAGTGATCACGTAGTTAATGGTCACTTCTGCCGCAGTACCTGCTGTGGTAGGAATTGCCAGAATCGGTaca                                                                      <  1:461590/151‑1 (MQ=255)
                                                                              catGTCAGCGTCAATAAACGCCACCTGCGGGATATCATGCGGATCAACGCAAACAAACTTGCGCCGCTTCTCTTCGTCAGTGATCACGTAGTTAATGGTCACTTCTGCCGCAGTACCTGCTGTGGTAGGAATTGCCAGAATCGGTACACt                                                                    <  2:940139/150‑1 (MQ=255)
                                                                              catGTCAGCGTCAATAAACGCCACCTGCGGGATATCATGCGGATCAACGCAAACAAACTTGCGCCGCTTCTCTTCGTCAGTGATCACGTAGTTAATGGTCACTTCTGCCGCAGTACCTGCTGTGGTAGGAATTGCCAGAATCGGTACACTg                                                                   <  1:15958/151‑1 (MQ=255)
                                                                              catGTCAGCGTCAATAAACGCCACCTGCGGGATATCATGCGGATCAACGCAAACAAACTTGCGCCGCTTCTCTTCGTCAGTGATCACGTAGTTAATGGTCACTTCTGCCGCAGTACCTGCTGTGGTAGGAATTGCCAGAATCGGTACACTg                                                                   >  2:19737/1‑151 (MQ=255)
                                                                               atGTCAGCGTCAATAAACGCCACCTGCGGGATATCATGCGGATCAACGCAAACAAACTTGCGCCGCTTCTCTTCGTCAGTGATCACGTAGTTAATGGTCACTTCTGCCGCAGTACCTGCTGTGGTAGGAATTGCCAGAATCGGTACACTgg                                                                  >  2:188073/1‑151 (MQ=255)
                                                                               atGTCAGCGTCAATAAACGCCACCTGCGGGATATCATGCGGATCAACGCAAACAAACTTGCGCCGCTTCTCTTCGTCAGTGATCACGTAGTTAATGGTCACTTCTGCCGCAGTACCTGCTGTGGTAGGAATTGCCAGAATCGGTACACTgg                                                                  <  2:1411884/151‑1 (MQ=255)
                                                                               atGTCAGCGTCAATAAACGCCACCTGCGGGATATCATGCGGATCAACGCAAACAAACTTGCGCCGCTTCTCTTCGTCAGTGATCACGTAGTTAATGGTCACTTCTGCCGCAGTACCTGCTGTGGTAGGAATTGCCAGAATCGGTACACTgg                                                                  <  2:77274/151‑1 (MQ=255)
                                                                                    aGCGTCAATAAACGCCACCTGCGGGATATCATGCGGATCAACGCAAACAAACTTGCGCCGCTTCTCTTCGTCAGTGATCACGTAGTTAATGGTCACTTCTGCCGCAGTACCTGCTGTGGTAGGAATTGCCAGAATCGGTACACTGGGTTTa                                                             <  1:1438250/151‑1 (MQ=255)
                                                                                      cGTCAATAAACGCCACCTGCGGGATATCATGCGGATCAACGCAAACAAACTTGCGCCGCTTCTCTTCGTCAGTGATCACGTAGTTAATGGTCACTTCTGCCGCAGTACCTGCTGTGGTAGGAATTGCCAGAATCGGTACACTGGGTTTAtt                                                           >  1:534095/1‑151 (MQ=255)
                                                                                           aTAAACGCCACCTGCGGGATATCATGCGGATCAACGCAAACAAACTTGCGCCGCTTCTCTTCGTCAGTGATCACGTAGTTAATGGTCACTTCTGCCGCAGTACCTGCTGTGGTAGGAATTGCCAGAATCGGTACACTGGGTTTATTGGTCg                                                      >  1:46037/1‑151 (MQ=255)
                                                                                              aaCGCCACCTGCGGGATATCATGCGGATCAACGCAAACAAACTTGCGCCGCTTCTCTTCGTCAGTGATCACGTAGTTAATGGTCACTTCTGCCGCAGTACCTGCTGTGGTAGGAATTGCCAGAATCGGTACACTGGGTTTATTGGTCgggg                                                   >  2:132664/1‑151 (MQ=255)
                                                                                                   cACCTGCGGGATATCATGCGGATCAACGCAAACAAACTTGCGCCGCTTCTCTTCGTCAGTGATCACGTAGTTAATGGTCACTTCTGCCGCAGTACCTGCTGTGGTAGGAATTGCCAGAATCGGTACACTGGGTTTATTGGTCGGGGAAAGc                                              >  1:107011/1‑151 (MQ=255)
                                                                                                          gggATATCATGCGGATCAACGCAAACAAACTTGCGCCGCTTCTCTTCGTCAGTGATCACGTAGTTAATGGTCACTTCTGCCGCAGTACCTGCTGTGGTAGGAATTGCCAGAATCGGTACACTGGGTTTATTGGTCGGGGAAAGCCCTTcc                                        >  2:1218091/1‑150 (MQ=255)
                                                                                                           ggATATCATGCGGATCAACGCAAACAAACTTGCGCCGCTTCTCTTCGTCAGTGATCACGTAGTTAATGGTCACTTCTGCCGCAGTACCTGCTGTGGTAGGAATTGCCAGAATCGGTACACTGGGTTTATTGGTCGGGGAAAGCCCTTCCAg                                      <  1:510254/151‑1 (MQ=255)
                                                                                                             atatCATGCGGATCAACGCAAACAAACTTGCGCCGCTTCTCTTCGTCAGTGATCACGTAGTTAATGGTCACTTCTGCCGCAGTACCTGCTGTGGTAGGAATTGCCAGAATCGGTACACTGGGTTTATTGGTCGGGGAAAGCCCTTCCAGGc                                    <  2:971421/151‑1 (MQ=255)
                                                                                                               atCATGCGGATCAACGCAAACAAACTTGCGCCGCTTCTCTTCGTCAGTGATCACGTAGTTAATGGTCACTTCTGCCGCAGTACCTGCTGTGGTAGGAATTGCCAGAATCGGTACACTGGGTTTATTGGTCGGGGAAAGCCCTTCCAGGCt                                   >  2:157408/1‑150 (MQ=255)
                                                                                                                 cATGCGGATCAACGCAAACAAACTTGCGCCGCTTCTCTTCGTCAGTGATCACGTAGTTAATGGTCACTTCTGCCGCAGTACCTGCTGTGGTAGGAATTGCCAGAATCGGTACACTGGGTTTATTGGTCGGGGAAAGCCCTTCCAGGCTACg                                >  2:1443301/1‑151 (MQ=255)
                                                                                                                        aTCAACGCAAACAAACTTGCGCCGCTTCTCTTCGTCAGTGATCACGTAGTTAATGGTCACTTCTGCCGCAGTACCTGCTGTGGTAGGAATTGCCAGAATCGGTACACTGGGTTTATTGGTCGGGGAAAGCCCTTCCAGGCTACGCACATCg                         >  1:701380/1‑151 (MQ=255)
                                                                                                                            aCGCAAACAAACTTGCGCCGCTTCTCTTCGTCAGTGATCACGTAGTTAATGGTCACTTCTGCCGCAGTACCTGCTGTGGTAGGAATTGCCAGAATCGGTACACTGGGTTTATTGGTCGGGGAAAGCCCTTCCAGGCTACGCACATCGGCaa                     <  2:762172/151‑1 (MQ=255)
                                                                                                                             cGCAAACAAACTTGCGCCGCTTCTCTTCGTCAGTGATCACGTAGTTAATGGTCACTTCTGCCGCAGTACCTGCTGTGGTAGGAATTGCCAGAATCGGTACACTGGGTTTATTGGTCGGGGAAAGCCCTTCCAGGCTACGCACATCGGCaa                     >  1:1698232/1‑150 (MQ=255)
                                                                                                                              gCAAACAAACTTGCGCCGCTTCTCTTCGTCAGTGATCACGTAGTTAATGGTCACTTCTGCCGCAGTACCTGCTGTGGTAGGAATTGCCAGAATCGGTACACTGGGTTTATTGGTCGGGGAAAGCCCTTCCAGGCTACGCACATCGGCaaa                    >  2:1585015/1‑150 (MQ=255)
                                                                                                                              gCAAACAAACTTGCGCCGCTTCTCTTCGTCAGTGATCACGTAGTTAATGGTCACTTCTGCCGCAGTACCTGCTGTGGTAGGAATTGCCAGAATCGGTACACTGGGTTTATTGGTCGGGGAAAGCCCTTCCAGGCTACGCACATCGGCAAAc                   <  2:1325742/151‑1 (MQ=255)
                                                                                                                              gCAAACAAACTTGCGCCGCTTCTCTTCGTCAGTGATCACGTAGTTAATGGTCACTTCTGCCGCAGTACCTGCTGTGGTAGGAATTGCCAGAATCGGTACACTGGGTTTATTGGTCGGGGAAAGCCCTTCCAGGCTACGCACATCGGCAAAc                   >  2:651532/1‑151 (MQ=255)
                                                                                                                               caaacaaaCTTGCGCCGCTTCTCTTCGTCAGTGATCACGTAGTTAATGGTCACTTCTGCCGCAGTACCTGCTGTGGTAGGAATTGCCAGAATCGGTACACTGGGTTTATTGGTCGGGGAAAGCCCTTCCAGGCTACGCACATCGGCAAACt                  <  1:852806/151‑1 (MQ=255)
                                                                                                                               caaacaaaCTTGCGCCGCTTCTCTTCGTCAGTGATCACGTAGTTAATGGTCACTTCTGCCGCAGTACCTGCTGTGGTAGGAATTGCCAGAATCGGTACACTGGGTTTATTGGTCGGGGAAAGCCCTTCCAGGCTACGCACATCGGCAAACt                  <  2:363471/151‑1 (MQ=255)
                                                                                                                               caaacaaaCTTGCGCCGCTTCTCTTCGTCAGTGATCACGTAGTTAATGGTCACTTCTGCCGCAGTACCTGCTGTGGTAGGAATTGCCAGAATCGGTACACTGGGTTTATTGGTCGGGGAAAGCCCTTCCAGGCTACGCACATCGGCAAACt                  <  1:614978/151‑1 (MQ=255)
                                                                                                                                  acaaacTTGCGCCGTTTCTCTTCGTCAGTGATCACGTAGTTAATGGTCACTTCTGCCGCAGTACCTGCTGTGGTAGGAATTGCCAGAATCGGTACACTGGGTTTATTGGTCGGGGAAAGCCCTTCCAGGCTACGCACATCGGCAAACTCCg               <  2:1598498/151‑1 (MQ=255)
                                                                                                                                   caaacTTGCGCCGCTTCTCTTCGTCAGTGATCACGTAGTTAATGGTCACTTCTGCCGCAGTACCTGCTGTGGTAGGAATTGCCAGAATCGGTACACTGGGTTTATTGGTCGGGGAAAGCCCTTCCAGGCTACGCACATCGGCAAACTCCgg              <  1:157400/151‑1 (MQ=255)
                                                                                                                                    aaacTTGCGCCGCTTCTCTTCGTCAGTGATCACGTAGTTAATGGTCACTTCTGCCGCAGTACCTGCTGTGGTAGGAATTGCCAGAATCGGTACACTGGGTTTATTGGTCGGGGAAAGCCCTTCCAGGCTACGCACATCGGCAAACTCCggg             >  2:1295127/1‑151 (MQ=255)
                                                                                                                                     aacTTGCGCCGCTTCTCTTCGTCAGTGATCACGTAGTTAATGGTCACTTCTGCCGCAGTACCTGCTGTGGTAGGAATTGCCAGAATCGGTACACTGGGTTTATTGGTCGGGGAAAGCCCTTCCAGGCTACGCACATCGGCAAACTCCGGgt            <  1:492823/151‑1 (MQ=255)
                                                                                                                                     aacTTGCGCCGCTTCTCTTCGTCAGTGATCACGTAGTTAATGGTCACTTCTGCCGCAGTACCTGCTGTGGTAGGAATTGCCAGAATCGGTACACTGGGTTTATTGGTCGGGGAAAGCCCTTCCAGGCTACGCACATCGGCAAACTCCGGgt            >  1:646307/1‑151 (MQ=255)
                                                                                                                                      acTTGCGCCGCTTCTCTTCGTCAGTGATCACGTAGTTAATGGTCACTTCTGCCGCAGTACCTGCTGTGGTAGGAATTGCCAGAATCGGTACACTGGGTTTATTGGTCGGGGAAAGCCCTTCCAGGCTACGCACATCGGCAAACTCCGGgtt           >  2:525664/1‑151 (MQ=255)
                                                                                                                                      acTTGCGCCGCTTCTCTTCGTCAGTGATCACGTAGTTAATGGTCACTTCTGCCGCAGTACCTGCTGTGGTAGGAATTGCCAGAATCGGTACACTGGGTTTATTGGTCGGGGAAAGCCCTTCCAGGCTACGCACATCGGCAAACTCCGGgtt           <  2:1563690/151‑1 (MQ=255)
                                                                                                                                          gcgcCGCTTCTCTTCGTCAGTGATCACGTAGTTAATGGTCACTTCTGCCGCAGTACCTGCTGTGGTAGGAATTGCCAGAATCGGTACACTGGGTTTATTGGTCGGGGAAAGCCCTTCCAGGCTACGCACATCGGCAAACTCCGGgttgtt        <  1:1695519/150‑1 (MQ=255)
                                                                                                                                               gTTTCTCTTCGTCAGTGATCACGTAGTTAATGGTCACTTCTGCCGCAGTACCTGCTGTGGTAGGAATTGCCAGAATCGGTACACTGGGTTTATTGGTCGGGGAAAGCCCTTCCAGGCTACGCACATCGGCAAACTCCGGGTTGTTGCTGAt  >  2:1653570/1‑151 (MQ=255)
                                                                                                                                                |                                                                                                                                                     
GGTAATATACCCCTCAATAGCATGAGTGAGCGCATCGACACCCGTCGCAGCTTTCAGCGCTGGAGGCATACCATCCATCATGTCAGCGTCAATAAACGCCACCTGCGGGATATCATGCGGATCAACGCAAACAAACTTGCGCCGTTTCTCTTCGTCAGTGATCACGTAGTTAATGGTCACTTCTGCCGCAGTACCTGCTGTGGTAGGAATTGCCAGAATCGGTACACTGGGTTTATTGGTCGGGGAAAGCCCTTCCAGGCTACGCACATCGGCAAACTCCGGGTTGTTGCTGAT  >  NC_000913/2932396‑2932689

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑