Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,065,614	T→C	R271R (CGT→CGC)	scpC →	propionyl‑CoA:succinate CoA transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,065,614	0	T	C	100.0%	281.4 / NA	78	R271R (CGT→CGC)	scpC	propionyl‑CoA:succinate CoA transferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (35/43);  total (35/43)

TGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAATCTCGGCCGATTCCCTGCGCAAGAT  >  NC_000913/3065466‑3065757                                                                                                                                                     |                                                                                                                                                tGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGCCt                                                                                                                                               >  2:1677110/1‑151 (MQ=255)    tGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGCCTggg                                                                                                                                            >  2:465539/1‑151 (MQ=255)     ggATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGCCTgggg                                                                                                                                           <  2:122236/151‑1 (MQ=255)       aTAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGCCTGGGGGa                                                                                                                                         <  2:862341/151‑1 (MQ=255)         aaGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGCCTGGGGGaaa                                                                                                                                       <  2:151466/151‑1 (MQ=255)           gCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGCCTGGGGGAAAAc                                                                                                                                     <  2:860375/151‑1 (MQ=255)            cAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGCCTGGGGGAAAAcc                                                                                                                                    <  2:196579/151‑1 (MQ=255)               aaTCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGCCTGGGGGAAAACCCgg                                                                                                                                 <  1:71552/151‑1 (MQ=255)                  cccATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGCCTGGGGGAAAACCCGGTaa                                                                                                                              <  2:1274294/151‑1 (MQ=255)                  cccATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGACAATATCAATAATGCGGTAATGGCGCGCCTGGGGGAAAACCCGGTaa                                                                                                                              <  2:1302961/151‑1 (MQ=255)                    cATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGCCTGGGGGAAAACCCGGTAAtt                                                                                                                            <  1:1099239/151‑1 (MQ=255)                         gCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATtcctcc                                                                                                                       >  2:1476811/1‑151 (MQ=255)                         gCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATtcctc                                                                                                                        <  2:111075/150‑1 (MQ=255)                           cagcagATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGt                                                                                                                     >  2:517125/1‑151 (MQ=255)                              cagATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGttt                                                                                                                   >  1:940755/1‑150 (MQ=255)                               agATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGTTTat                                                                                                                 <  1:223538/151‑1 (MQ=255)                                      cGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTAt                                                                                                          >  2:1155387/1‑151 (MQ=255)                                      cGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTAt                                                                                                          <  2:380557/151‑1 (MQ=255)                                           aCGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGGAATGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCgg                                                                                                      >  1:547330/1‑150 (MQ=255)                                            cGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGaa                                                                                                    <  1:1393695/151‑1 (MQ=255)                                             gTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGCCTGGGGGAAATCCCGGTAATTCCTCCGTTTATGATGTATTCGGAAg                                                                                                   <  2:1065801/151‑1 (MQ=255)                                               ggTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTg                                                                                                 <  2:1694048/151‑1 (MQ=255)                                                gTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGc                                                                                                <  2:570153/151‑1 (MQ=255)                                                   aCGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTa                                                                                              >  1:784109/1‑150 (MQ=255)                                                    cGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTAc                                                                                             >  2:69021/1‑150 (MQ=255)                                                     gTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACa                                                                                            <  1:691656/150‑1 (MQ=255)                                                     gTTCTTATTGCAGGAAATGGCGCATGGGCGTATCCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGATTATGATGTATTCGGTAGTGCTAc                                                                                             >  1:85349/1‑149 (MQ=255)                                                        cTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGa                                                                                         >  2:1664547/1‑150 (MQ=255)                                                          tATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGaa                                                                                        <  1:450888/149‑1 (MQ=255)                                                            ttGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGGATCgg                                                                                    >  1:688246/1‑151 (MQ=255)                                                            ttGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCgg                                                                                    >  2:766924/1‑151 (MQ=255)                                                             tGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCggt                                                                                   >  2:196913/1‑151 (MQ=255)                                                              gCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCggt                                                                                   <  2:386764/150‑1 (MQ=255)                                                               cAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCggtgg                                                                                 <  1:1317646/151‑1 (MQ=255)                                                                aGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCggtggt                                                                                <  1:360763/151‑1 (MQ=255)                                                                    aaTGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCAt                                                                            >  2:714942/1‑151 (MQ=255)                                                                      tGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCAttt                                                                          >  1:1513927/1‑151 (MQ=255)                                                                       ggCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCAttt                                                                          >  1:53638/1‑150 (MQ=255)                                                                       ggCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTa                                                                         <  1:1476713/151‑1 (MQ=255)                                                                        gcgcATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCAttt                                                                          >  1:26289/1‑149 (MQ=255)                                                                         cgcATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACt                                                                       >  1:911584/1‑151 (MQ=255)                                                                         cgcATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACt                                                                       <  1:1342271/151‑1 (MQ=255)                                                                            aTGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTgg                                                                     >  1:432938/1‑150 (MQ=255)                                                                              gggCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGaa                                                                   <  1:403495/150‑1 (MQ=255)                                                                                            ggAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGc                                                    <  1:615499/151‑1 (MQ=255)                                                                                               aTTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCggg                                                 <  2:1509677/151‑1 (MQ=255)                                                                                               aTTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCggg                                                 >  2:1570978/1‑151 (MQ=255)                                                                                                    tGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGcc                                              >  2:23590/1‑149 (MQ=255)                                                                                                    tGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGcc                                              >  1:730596/1‑149 (MQ=255)                                                                                                     gCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCa                                             <  2:225277/149‑1 (MQ=255)                                                                                                           gCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCt                                       <  2:1279618/149‑1 (MQ=255)                                                                                                            cAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCa                                    <  1:1522729/151‑1 (MQ=255)                                                                                                              aaGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGc                                  >  1:445116/1‑151 (MQ=255)                                                                                                              aaGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGc                                  <  1:1187908/151‑1 (MQ=255)                                                                                                                 tGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTg                               >  1:1674690/1‑151 (MQ=255)                                                                                                                  ggCGTGGGCAATATCAATAATGCGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTg                               <  2:1213215/150‑1 (MQ=255)                                                                                                                  ggCGTGGGCAATATCAATAATGCGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGa                              <  2:547379/151‑1 (MQ=255)                                                                                                                  ggCGTGGGCAATATCAATAATGCGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGa                              <  1:991537/151‑1 (MQ=255)                                                                                                                       gggCAATATCAATAATGCGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAAtc                         >  2:1424565/1‑151 (MQ=255)                                                                                                                        ggCAATATCAATAATGCGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAAtct                        <  1:856339/151‑1 (MQ=255)                                                                                                                         gCAATATCAATAATGCGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAAtctc                       >  1:308766/1‑151 (MQ=255)                                                                                                                           aaTATCAATAATGCGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAATCTCgg                     >  2:237149/1‑151 (MQ=255)                                                                                                                           aaTATCAATAATGCGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAATCTCgg                     <  1:1165433/151‑1 (MQ=255)                                                                                                                           aaTATCAATAATGCGGTAATGGCGCGCCTGGGGGAAAACCCGGT‑ATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAGCCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAATCTCGGc                    <  2:144818/151‑1 (MQ=255)                                                                                                                            atatCAATAATGCGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAATCTCGGc                    >  2:1536410/1‑151 (MQ=255)                                                                                                                              atCAATAATGCGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAATCTCGGcc                   <  2:1548233/150‑1 (MQ=255)                                                                                                                              atCAATAATGCGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAATCCCGGCCg                  >  2:158689/1‑151 (MQ=255)                                                                                                                                 aataatGCGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAATCTCGGCCGAtt               <  1:69018/151‑1 (MQ=255)                                                                                                                                      tGCGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAATCTCGGCCGATTCCCt           <  2:194186/150‑1 (MQ=255)                                                                                                                                       gCGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAATCTCGGCCGATTCCCTgc         >  2:1624946/1‑151 (MQ=255)                                                                                                                                       gCGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAATCTCGGCCGATTCCCTgc         >  1:593978/1‑151 (MQ=255)                                                                                                                                        cGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAATCTCGGCCGATTCCCTgcg        <  1:1545753/151‑1 (MQ=255)                                                                                                                                        cGGTAATGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAATCTCGGCCGATTCCCTgcg        >  1:843733/1‑151 (MQ=255)                                                                                                                                        cGGTAATGGCGCGCCTGGGGGAAAACCCGGGAATTCCTCCGTTTATGATGTATTCGGAAGTGCGACAGGAATCGGTGGTGCATGTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAATCTCGGCCGATTCCCTg          <  1:614820/149‑1 (MQ=255)                                                                                                                                            aaTGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAATCTCGGCCGATTCCCTGCGCaa     <  1:1112707/150‑1 (MQ=255)                                                                                                                                             aTGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAATCTCGGCCGATTCCCTGCGCa      >  1:314253/1‑148 (MQ=255)                                                                                                                                             aTGGCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCAGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAATCTCGGCCGATTCCCTGCGCa      >  2:1605896/1‑148 (MQ=255)                                                                                                                                               ggCGCGCCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAATCTCGGCCGATTCCCTGCGCAAGAt  <  1:53113/150‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                TGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCGGAATTTCTGCCGCTGCAAAGTGGCGTGGGCAATATCAATAATGCGGTAATGGCGCGTCTGGGGGAAAACCCGGTAATTCCTCCGTTTATGATGTATTCGGAAGTGCTACAGGAATCGGTGGTGCATTTACTGGAAACCGGCAAAATCAGCGGGGCCAGCGCCTCCAGCCTGACAATCTCGGCCGATTCCCTGCGCAAGAT  >  NC_000913/3065466‑3065757

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑