| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,272,271 | Δ1 bp | coding (481/885 nt) | garR ← | tartronate semialdehyde reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,272,268 | 0 | C | . | 97.1% | 159.8 / ‑1.6 | 35 | coding (484/885 nt) | garR | tartronate semialdehyde reductase |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base . (16/18); total (16/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.16e-01 | |||||||||||
CAGTCCACCGCGAATTGCCTGATAAACCAGGTCCGGGTTAACGCCCGCTTTAGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTTCCCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGATGGCTTTCGGTTCACCGCCGCTCACCGGAGCATCCAGCAT > NC_000913/3272125‑3272409 | cAGTCCACCGCGAATTGCCTGATAAACCAGGTCCGGGTTAACGCCCGCTTTAGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTT‑CCCGGTAt < 2:360336/151‑1 (MQ=255) tCCACCGCGAATTGCCTGATAAACCAGGTCCGGGTTAACGCCCGCTTTAGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTT‑CCCGGTATGc > 1:118737/1‑150 (MQ=255) aCCGCGAATTGCCTGATAAACCAGGTCCGGGTTAACGCCCGCTTTAGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTT‑CCCGGTATGcacca < 2:460653/151‑1 (MQ=255) gAATTGCCTGATAAACCAGGTCCGGGTTAACGCCCGCTTTAGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTT‑CCCGGTATGCACCACGGaa < 1:457515/151‑1 (MQ=255) gATAAACCAGGTCCGGGTTAACGCCCGCTTTAGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCa < 2:260948/149‑1 (MQ=255) gATAAACCAGGTCCGGGTTAACGCCCGCTTTAGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCa < 2:370045/149‑1 (MQ=255) cAGGTCCGGGTTAACGCCCGCTTTAGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCATCGCTTtca < 2:391408/151‑1 (MQ=255) ccGGGTTAACGCCCGCTTTAGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCaa < 2:554670/150‑1 (MQ=255) gTTAACGCCCGCTTTAGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCAt > 2:546610/1‑151 (MQ=255) tAACGCCCGCTTTAGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCAt > 2:390209/1‑149 (MQ=255) aaCGCCCGCTTTAGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGt < 1:146676/151‑1 (MQ=255) gCCCGCTTTAGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTAtt < 2:505691/151‑1 (MQ=255) tAGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGaa < 1:73219/150‑1 (MQ=255) aGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGaaa > 2:443101/1‑150 (MQ=255) tGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTTCCCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAAt < 2:473425/150‑1 (MQ=255) tGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATa < 2:242911/150‑1 (MQ=255) gCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGc < 2:599489/151‑1 (MQ=255) gCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTg > 2:362003/1‑151 (MQ=255) cGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGt < 1:221193/151‑1 (MQ=255) tCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTCGCCGCcca < 2:136251/150‑1 (MQ=255) tGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTCGCCGCccacc > 2:309067/1‑150 (MQ=255) gcgGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTCGCCGCCCACCATCACTg > 2:299968/1‑150 (MQ=255) ttCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTAcc > 1:473686/1‑151 (MQ=255) gcgcCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCg < 2:108680/151‑1 (MQ=255) cacaATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGATgg > 1:112123/1‑151 (MQ=255) cacaATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGATgg > 2:59007/1‑151 (MQ=255) acaATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGATGGc > 1:270012/1‑151 (MQ=255) caATGACCTGATTTGCCAGTTGGGTGACGTTACCTGCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGATGGCt < 2:59140/151‑1 (MQ=255) gACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGATGGCTTTCg > 1:456547/1‑151 (MQ=255) tGCCAGTTTGGTGACGTTACCTGCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGATGGCTTTCGGTTCAccgc > 2:528868/1‑151 (MQ=255) ttGGTGACGTTACCTGCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGATGGCTTTCGGTTCACCGCCGCTCAc > 1:91471/1‑151 (MQ=255) aCCTGCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGATGGCTTTCGGTTCACCGCCGCTCACCGGAGCATcc < 2:112123/150‑1 (MQ=255) ccTGCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGATGGCTTTCGGTTCACCGCCGCTCACCGGAGCATcc > 1:264098/1‑149 (MQ=255) ccTGCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGATGGCTTTCGGTTCACCGCCGCTCACCGGAGCATCCa < 1:270259/150‑1 (MQ=255) gCACCGATTT‑CCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGATGGCTTTCGGTTCACCGCCGCTCACCGGAGCATCCAGCat > 1:206972/1‑151 (MQ=255) | CAGTCCACCGCGAATTGCCTGATAAACCAGGTCCGGGTTAACGCCCGCTTTAGTTGCCAGCGTTAACGCTTCTGACATCGCGGCAATATTCAGCGCCACAATGACCTGATTTGCCAGTTTGGTGACGTTACCTGCACCGATTTCCCCGGTATGCACCACGGAACCCGCCATCGCTTTCATCAAATCATAGTATTTGTCGAAAATAGCCTTGTCGCCGCCCACCATCACTGACAGCGTACCGTCGATGGCTTTCGGTTCACCGCCGCTCACCGGAGCATCCAGCAT > NC_000913/3272125‑3272409 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |