| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,399,151 | Δ1 bp | coding (1740/1848 nt) | mutL → | methyl‑directed mismatch repair protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,399,151 | 0 | G | . | 97.6% | 188.6 / ‑1.3 | 41 | coding (1740/1848 nt) | mutL | methyl‑directed mismatch repair protein |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base . (17/23); total (17/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
CAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCGATAAAAGCCCTGAAAGATGAGTGATATCAGTAAGGCGAGCCTGCCTAAGGCGATTTTTTTG > NC_000913/4399005‑4399296 | caacaaAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGc > 2:234084/1‑151 (MQ=255) acaaAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCg < 1:91790/151‑1 (MQ=255) acaaAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCgg < 2:581628/151‑1 (MQ=255) aaaTTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGa < 1:44434/149‑1 (MQ=255) aaaTTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACg < 2:475775/151‑1 (MQ=255) aaTTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGt > 1:599125/1‑151 (MQ=255) tACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGa > 2:422608/1‑150 (MQ=255) gATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGt < 2:325685/150‑1 (MQ=255) gATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGt < 1:66294/150‑1 (MQ=255) aTTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTcc > 1:547443/1‑151 (MQ=255) ttCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCg > 2:30730/1‑151 (MQ=255) tCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCg < 2:493054/150‑1 (MQ=255) aaCTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACtt < 2:370334/151‑1 (MQ=255) aaCTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACtt > 2:614294/1‑151 (MQ=255) aCCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAAcgcc < 2:356895/150‑1 (MQ=255) aCCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAAcgc < 2:488601/149‑1 (MQ=255) tGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGccgccg > 1:205256/1‑151 (MQ=255) tCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGtt > 1:370733/1‑151 (MQ=255) cGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTg < 1:10889/151‑1 (MQ=255) cGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTg < 1:422615/151‑1 (MQ=255) gAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGt > 1:479618/1‑151 (MQ=255) aCCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTg > 1:268201/1‑151 (MQ=255) gCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGAttt > 2:553850/1‑150 (MQ=255) aaTATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTAc > 2:255682/1‑150 (MQ=255) aaTATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACa < 1:345482/151‑1 (MQ=255) tatTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATc > 2:592418/1‑151 (MQ=255) atTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATc < 1:385908/150‑1 (MQ=255) cgcAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCg < 1:390125/151‑1 (MQ=255) gcAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCg > 1:373222/1‑150 (MQ=255) gcAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCGa < 1:566338/151‑1 (MQ=255) gTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCGATa < 2:140602/150‑1 (MQ=255) ggATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCGATaaaa < 2:571529/151‑1 (MQ=255) gCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCGATAAAAGCCCt > 2:630869/1‑151 (MQ=255) gAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCGATAAAAGCCCTGaaa < 1:625977/151‑1 (MQ=255) aaTCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCGATAAAAGCCCTGAAAGa < 1:185512/151‑1 (MQ=255) gatgaGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCGATAAAAGCCCTGAAAGATGAg < 1:586743/150‑1 (MQ=255) tgaGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCGATAAAAGCCCTGAAAGATGAGTg > 1:58626/1‑150 (MQ=255) gaGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCGATAAAAGCCCTGAAAGATGAGTGat < 2:583123/151‑1 (MQ=255) aTGCGCAGTGGTCAATGGCACAGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCGATAAAAGCCCTGAAAGATGAGTGATGTCAGTaa < 1:490443/151‑1 (MQ=255) cacaGGCCATAACCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCGATAAAAGCCCTGAAAGATGAGTGATATCAGTAAGGCGAGCCTGCCTAAGGc < 1:614311/151‑1 (MQ=255) aaCCCT‑CTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCGATAAAAGCCCTGAAAGATGAGTGATATCAGTAAGGCGAGCCTGCCTAAGGCGATTTTTTTg > 2:523678/1‑151 (MQ=255) | CAACAAAATTTACAAATCTTGATTCCTGAACTGATAGGCTACCTGGCGAAGCAGTCCGTATTCGAACCTGGCAATATTGCGCAGTGGATTGCACGAAATCTGATGAGCGAACATGCGCAGTGGTCAATGGCACAGGCCATAACCCTGCTGGCGGACGTGGAACGGTTATGTCCGCAACTTGTGAAAACGCCGCCGGGTGGTCTGTTACAATCTGTTGATTTACATCCGGCGATAAAAGCCCTGAAAGATGAGTGATATCAGTAAGGCGAGCCTGCCTAAGGCGATTTTTTTG > NC_000913/4399005‑4399296 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |