Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,449,635	(C)5→6	coding (415/822 nt)	yfcO ←	DUF2544 family putative outer membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,449,630	1	.	C	100.0%	132.0 / NA	38	G140G (GGC→GGG)	yfcO	DUF2544 family putative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (15/23);  total (15/23)

CAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACG‑CCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCACCCAGACGGTAATCCAGGACAACTTG  >  NC_000913/2449494‑2449766                                                                                                                                          |                                                                                                                                         cAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGagag                                                                                                                              <  1:177623/150‑1 (MQ=255)   aCCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCt                                                                                                                           >  2:445328/1‑151 (MQ=255)    ccACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCtt                                                                                                                          <  1:585782/151‑1 (MQ=255)      acacCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCa                                                                                                                        <  2:215689/151‑1 (MQ=255)      acacCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCa                                                                                                                        <  2:104499/151‑1 (MQ=255)            cccGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGtca                                                                                                                  <  2:509656/151‑1 (MQ=255)                 cGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGtcattcat                                                                                                             <  2:67419/151‑1 (MQ=255)                   aCTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCataa                                                                                                           <  2:235550/151‑1 (MQ=255)                      gCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCataata                                                                                                         <  2:386079/150‑1 (MQ=255)                             gAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATaaccaacc                                                                                                  <  1:59749/150‑1 (MQ=255)                                   ttttGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGAc                                                                                            >  1:437011/1‑150 (MQ=255)                                   ttttGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGAc                                                                                            >  1:464731/1‑150 (MQ=255)                                     ttGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACaa                                                                                          <  2:314930/150‑1 (MQ=255)                                        aCTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGaa                                                                                       >  2:451995/1‑150 (MQ=255)                                           tACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAg                                                                                    >  2:506656/1‑150 (MQ=255)                                               gACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGa                                                                               <  1:86929/151‑1 (MQ=255)                                                aCATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTa                                                                                  >  2:80768/1‑147 (MQ=255)                                                  aTAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTc                                                                            >  2:318576/1‑151 (MQ=255)                                                   tAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTc                                                                            >  2:30585/1‑150 (MQ=255)                                                    aaTTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCa                                                                           <  2:138577/150‑1 (MQ=255)                                                           aTTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGtcatt                                                                                                                <  2:109776/106‑1 (MQ=255)                                                           aTTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGtcatt                                                                                                                >  1:109776/1‑106 (MQ=255)                                                              ccATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGc                                                                   >  2:532591/1‑148 (MQ=255)                                                               cATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCagag                                                               <  2:225163/151‑1 (MQ=255)                                                                  gAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGAc                                                             <  1:172332/150‑1 (MQ=255)                                                                               gTTGAACAGAAACGGATGGACAAATCGTGTGGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGACATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAAt                                                >  2:386677/1‑150 (MQ=255)                                                                                  gAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGtat                                            <  1:359103/151‑1 (MQ=255)                                                                                  gAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGtat                                            <  1:221526/151‑1 (MQ=255)                                                                                                 gACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTcc                             <  1:59949/151‑1 (MQ=255)                                                                                                   cAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCAc                           >  1:275529/1‑151 (MQ=255)                                                                                                         gtgtcgtgACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCACCCAGAc                     <  2:479573/151‑1 (MQ=255)                                                                                                         gtgtcgtgACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCACCCAGAc                     <  2:56032/151‑1 (MQ=255)                                                                                                           gtcgtgACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCACCCAGACgg                   >  1:314737/1‑151 (MQ=255)                                                                                                             cgtgACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCACCCAGACGGTa                 <  2:86669/151‑1 (MQ=255)                                                                                                                gACTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCACCCAGACGGTAATc              >  2:465952/1‑151 (MQ=255)                                                                                                                 aCTATCAGGCCCCTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCACCCAGACGGTAATcc             <  1:381404/151‑1 (MQ=255)                                                                                                                           cccTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCACCCAGACGGTAATCCAGGACAACtt   <  1:402140/151‑1 (MQ=255)                                                                                                                             cTGATACGTCACGCCCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCACCCAGACGGTAATCCAGGACAACTTg  >  1:354668/1‑150 (MQ=255)                                                                                                                                          |                                                                                                                                         CAACCACACCGCCCGTCGACTGCAGGGTGAGCAGTTTTGACTTACTGACATAATTCTCATTCCATGAAACGTCATAAGGTTGAACAGAAACGGATGGACAAATCGTGTCGTGACTATCAGGCCCCTGATACGTCACG‑CCCCCTGGAGAGGCTTCAGAGTCATTCATAATAACCAACCATGGACAAGAAAAGTAAGATTCAATCTCTGCAGAGACAATAACCTCAAATGTATAATACCCGCAGTTCCACCCAGACGGTAATCCAGGACAACTTG  >  NC_000913/2449494‑2449766

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑