Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	944,912	Δ1 bp :: IS186 (+) +6 bp :: Δ1 bp	intergenic (+16/+14)	dmsC → / ← ycaC	dimethyl sulfoxide reductase, anaerobic, subunit C/putative isochorismatase family hydrolase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	608008 =	NA (NA)	38 (0.960)	36/292	0.3	100%	noncoding (2/1345 nt)	IS186	repeat region
	?	NC_000913	= 944917	0 (0.000)					intergenic (+21/+14)	dmsC/ycaC	dimethyl sulfoxide reductase, anaerobic, subunit C/putative isochorismatase family hydrolase
*	?	NC_000913	= 609350	NA (NA)	46 (1.170)	45/292	0.1	100%	noncoding (1344/1345 nt)	IS186	repeat region
	?	NC_000913	944912 =	0 (0.000)					intergenic (+16/+19)	dmsC/ycaC	dimethyl sulfoxide reductase, anaerobic, subunit C/putative isochorismatase family hydrolase

GGTTAGAGCCCCGGCATTACGTGCCGAAGTATCCAGTTCTTCGGGCTTACCAATATGGGCCAGAATTGCTGACCAGTTATCGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/608160‑608008 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gggCCGAAACCCCGGTTGTTATGATTAGCTTGCGACGGCCATCCCCACGGTCATATGCAAACCATAGAATACGCCGCGACCGATTAATTCCCCTGCCAGAAGCAGAATGAATGATACAGAAAGTAGCGGAACCGCAGGCTGAT  <  NC_000913/944917‑944778                                                                                                                                                                                                                                                                                                       GGTTAGAGCCCCGGCATTACGTGCCGAAGTATCCAGTTCTTCGGGCTTACCAATATGGGCCAGAATTGCTGACCAGTTATCGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATG                                                                                                                                                >  2:430285/1‑151  GTTAGAGCCCCGGCATTACGTGCCGAAGTATCCAGTTCTTCGGGCTTACCAATATGGGCCAGAATTGCTGACCAGTTATCGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGG                                                                                                                                               <  2:404279/151‑1    TAGAGCCCCGGCATTACGTGCCGAAGTATCCAGTTCTTCGGGCTTACCAATATGGGCCAGAATTGCTGACCAGTTATCGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGG                                                                                                                                              <  2:6973/150‑1    TAGAGCCCCGGCATTACGTGCCGAAGTATCCAGTTCTTCGGGCTTACCAATATGGGCCAGAATTGCTGACCAGTTATCGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGG                                                                                                                                              <  2:44121/150‑1         CCCCGGCATTACGTGCCGAAGTATCCAGTTCTTCGGGCTTACCAATATGGGCCAGAATTGCTGACCAGTTATCGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGCCGAAA                                                                                                                                        <  2:521403/151‑1                     GTGCCGAAGTATCCAGTTCTTCGGGCTTACCAATATGGGCCAGAATTGCTGACCAGTTATCGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATGCCACAACCCTTAAGTTAGCGCTTATGGGCCGAAACCCCGGTTGTT                                                                                                                             <  2:439012/150‑1                         CGAAGTATCCAGTTCTTCGGGCTTACCAATATGGGCCAGAATTGCTGACCAGTTATCGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGCCGAAACCCCGGTTGTTATGAT                                                                                                                        >  1:470712/1‑151                            AGTATCCAGTTCTTCGGGCTTACCAATATGGGCCAGAATTGCTGACCAGTTATCGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGCCGAAACCCCGGTTGTTATGATTAG                                                                                                                     <  2:109352/151‑1                               ATCCAGTTCTTCGGGCTTACCAATATGGGCCAGAATTGCTGACCAGTTATCGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGCCGAAACCCCGGTTGTTATGATTAGCTT                                                                                                                  >  2:543145/1‑151                                         TCGGGCTTACCAATATGGGCCAGAATTGCTGACCAGTTATCGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGCCGAAACCCCGGTTGTTATGATTAGCTTGCGACGGC                                                                                                          <  1:389803/149‑1                                                       ATGGGCCAGAATTGCTGACCAGTTATCGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGCCGAAACCCCGGTTGTTATGATTAGCTTGCGACGGCCATCCCCACGGTCAT                                                                                           <  1:565218/150‑1                                                         GGGCCAGAATTGCTGACCAGTTATCGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGCCGAAACCCCGGTTGTTATGATTAGCTTGCGACGGCCATCCCCACGGTCATATG                                                                                        <  2:30702/151‑1                                                            CCAGAATTGCTGACCAGTTATCGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGCCGAAACCCCGGTTGTTATGATTAGCTTGCGACGGCCATCCCCACGGTCATATGCAA                                                                                     <  2:458243/151‑1                                                            CCAGAATTGCTGACCAGTTATCGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGCCGAAACCCCGGTTGTTATGATTAGCTTGCGACGGCCATCCCCACGGTCATATGCA                                                                                      >  1:326725/1‑150                                                              AGAATTGCTGACCAGTTATCGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGCCGAAACCCCGGTTGTTATGATTAGCTTG                                                                                                                 >  1:632454/1‑121                                                              AGAATTGCTGACCAGTTATCGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGCCGAAACCCCGGTTGTTATGATTAGCTTG                                                                                                                 <  2:632436/121‑1                                                                AATTGCTGACCAGTTATCGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGCCGAAACCCCGGTTGTTATGATTAGCTTGCGACGGCCATCCCCACGGTCATATGCAAACC                                                                                  >  1:510752/1‑150                                                                         CCAGTTATCGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGCCGAAACCCCGGTTGTTATGATTAGCTTGCGACGGCCATCCCCACGGTCATATGCAAACCATAGAATACG                                                                        <  2:194735/151‑1                                                                            GTTATCGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGCCGAAACCCCGGTTGTTATGATTAGCTTGCGACGGCCATCCCCACGGTCATATGCAAACCATAGAATACGCC                                                                      >  2:62532/1‑150                                                                                TCGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGCCGAAACCCCGGTTGTTATGATTAGCTTGCGACGGCCATCCCCACGGTCATATGCAAACCATAGAATACGCCGCGAC                                                                 >  2:602434/1‑151                                                                                 CGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGCCGAAACCCCGGTTGTTATGATTAGCTTGCGACGGCCATCCCCACGGTCATATGCAAACCATAGAATACGCCGCGACC                                                                <  1:425915/151‑1                                                                                  GTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGCCGAAACCCCGGTTGTTATGATTAGCTTGCGACGGCCATCCCCACGGTCATATGCAAACCATAGAATACGCCGCGACCG                                                               >  1:273928/1‑151                                                                                        GTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGCCGAAACCCCGGTTGTTATGATTAGCTTGCGACGGCCATCCCCACGGTCATATGCAAACCATAGAATACGCCGCGACCGATTAAT                                                         >  2:139820/1‑151                                                                                          AATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGCCGAAACCCCGGTTGTTATGATTAGCTTGCGACGGCCATCCCCACGGTCATATGCAAACCATAGAATACGCCGCGACCGATTAATT                                                        <  1:165858/150‑1                                                                                           ATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGCCGAAACCCCGGTTGTTATGATTAGCTTGCGACGGCCATCCCCACGGTCATATGCAAACCATAGAATACGCCGCGACCGATTAATTCC                                                      >  1:528209/1‑151                                                                                               ATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGCCGAAACCCCGGTTGTTATGATTAGCTTGCGACGGCCATCCCCACGGTCATATGCAAACCATAGAATACGCCGCGACCGATTAATTCCCCTG                                                  <  2:478210/151‑1                                                                                                    CACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGCCGAAACCCCGGTTGTTATGATTAGCTTGCGACGGCCATCCCCACGGTCATATGCAAACCATAGAATACGCCGCGACCGATTAATTCCCCTGCC                                                >  1:66268/1‑148                                                                                                             TCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGCCGAAACCCCGGTTGTTATGATTAGCTTGCGACGGCCATCCCCACGGTCATATGCAAACCATAGAATACGCCGCGACCGATTAATTCCCCTGCCAGAAGCAGAAT                                     >  2:488655/1‑150                                                                                                              CATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGCCGAAACCCCGGTTGTTATGATTAGCTTGCGACGGCCATCCCCACGGTCATATGCAAACCATAGAATACGCCGCGACCGATTAATTCCCCTGCCAGAAGCAGAATG                                    <  2:445660/150‑1                                                                                                                    AGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGCCGAAACCCCGGTTGTTATGATTAGCTTGCGACGGCCATCCCCACGGTCATATGCAAACCATAGAATACGCCGCGACCGATTAATTCCCCTGCCAGAAGCAGAATGAATGAT                              >  2:497075/1‑150                                                                                                                    AGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGCCGAAACCCCGGTTGTTATGATTAGCTTGCGACGGCCATCCCCACGGTCATATGCAAACCATAGAATACGCCGCGACCGATTAATTCCCCTGCCAGAAGCAGAATGAATGAT                              >  1:328973/1‑150                                                                                                                     GGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGGCCGAAACCCCGGTTGTTATGATTAGCTTGCGACGGCCATCCCCACGGTCATATGCAAACCATAGAATACGCCGCGACCGATTAATTCCCCTGCCAGAAGCAGAATGAATGATAC                            >  1:78808/1‑151                                                                                                                         TAATACCACAACCCTTAAGTTAGCGCTTATGGGCCGAAACCCCGGTTGTTATGATTAGCTTGCGACGGCCATCCCCACGGTCATATGCAAACCATAGAATACGCCGCGACCGATTAATTCCCCTGCCAGAAGCAGAATGAATGATACAGA                         >  2:350961/1‑150                                                                                                                              CCACAACCCTTAAGTTAGCGCTTATGGGCCGAAACCCCGGTTGTTATGATTAGCTTGCGACGGCCATCCCCACGGTCATATGCAAACCATAGAATACGCCGCGACCGATTAATTCCCCTGCCA                                               <  1:10334/123‑1                                                                                                                              CCACAACCCTTAAGTTAGCGCTTATGGGCCGAAACCCCGGTTGTTATGATTAGCTTGCGACGGCCATCCCCACGGTCATATGCAAACCATAGAATACGCCGCGACCGATTAATTCCCCTGCCA                                               >  2:10334/1‑123                                                                                                                                  AACCCTTAAGTTAGCGCTTATGGGCCGAAACCCCGGTTGTTATGATTAGCTTGCGACGGCCATCCCCACGGTCATATGCAAACCATAGAATACGCCGCGACCGATTAATTCCCCTGCCAGAAGCAGAATGAATGATACAGAAAGTAGCGG                >  2:335365/1‑150                                                                                                                                       TTAAGTTAGCGCTTATGGGCCGAAACCCCGGTTGTTATGATTAGCTTGCGACGGCCATCCCCACGGTCATATGCAAACCATAGAATACGCCGCGACCGATTAATTCCCCTGCCAGAAGCAGAATGAATGATACAGAAAGTAGCGGAACCGC          >  1:342967/1‑151                                                                                                                                       TTAAGTTAGCGCTTATGGGCCGAAACCCCGGTTGTTATGATTAGCTTGCGACGGCCATCCCCACGGTCATATGCAAACCATAGAATACGCCGCGACCGATTAATTCCCCTGCCAGAAGCAGAATGAATGATACAGAAAGTAGCGGAACCGC          >  2:282156/1‑151                                                                                                                                           GTTAGCGCTTATGGGCCGAAACCCCGGTTGTTATGATTAGCTTGCGACGGCCATCCCCACGGTCATATGCAAACCATAGAATACGCCGCGACCGATTAATTCCCCTGCCAGAAGCAGAATGAATGATACAGAAAGTAGCGGAACCGCAGGC      <  1:528340/151‑1                                                                                                                                                CGCTTATGGGCCGAAACCCCGGTTGTTATGATTAGCTTGCGACGGCCATCCCCACGGTCATATGCAAACCATAGAATACGCCGCGACCGATTAATTCCCCTGCCAGAAGCAGAATGAATGATACAGAAAGTAGCGGAACCGCAGGCTGAT  >  2:33544/1‑150                                                                                                                                                    TATGGGCCGAAACCCCGGTTGTTATGATTAGCTTGCGACGGCCATCCCCACGGTCATATGCAAACCATAGAATACGCCGCGACCGATTAATTCCCCTGCCAGAAGCAGAATGAATGATACAGAAAGTAGCGGAACCGCAGGCTGA   <  1:122648/145‑1                                                                                                                                                    TATGGGCCGAAACCCCGGTTGTTATGATTAGCTTGCGACGGCCATCCCCACGGTCATATGCAAACCATAGAATACGCCGCGACCGATTAATTCCCCTGCCAGAAGCAGAATGAATGATACAGAAAGTAGCGGAACCGCAGGCTGA   >  2:122646/1‑145                                                                                                                                                                                                                                                                                                       GGTTAGAGCCCCGGCATTACGTGCCGAAGTATCCAGTTCTTCGGGCTTACCAATATGGGCCAGAATTGCTGACCAGTTATCGTGAGAGTAATTCATCGGCACGTTAAATCATATCAGGCGTAATACCACAACCCTTAAGTTAGCGCTTATGGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/608160‑608008 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gggCCGAAACCCCGGTTGTTATGATTAGCTTGCGACGGCCATCCCCACGGTCATATGCAAACCATAGAATACGCCGCGACCGATTAATTCCCCTGCCAGAAGCAGAATGAATGATACAGAAAGTAGCGGAACCGCAGGCTGAT  <  NC_000913/944917‑944778

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.