Predicted mutation
evidence seq id position mutation annotation gene description
JC NC_000913 4,284,437 (CCA)4→3 coding (464‑466/1650 nt) actP ← acetate transporter

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 4284431 =1 (0.020)42 (1.110) 39/280 0.1 97.7% coding (472/1650 nt) actP acetate transporter
?NC_000913 = 4284427 1 (0.030)coding (476/1650 nt) actP acetate transporter

TTACTCGCTGGGCTTCCTGGTGGGCTGGCCGATCATTTTGTTCCTGATTGCCGAACGTCTGCGTAACCTGGGGCGCTACACCTTTGCCGATGTGGCCTCTTACCGTCTGAAACAAGGGCCGATTCGTATTCTTTCGGCCTGTGGTTCTCTGGTGGTGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/4284588‑4284431
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tggtggtggCGCTTTACCTTATCGCCCAGATGGTGGGCGCAGGTAAACTGATCGAGCTGCTGTTTGGCCTTAACTATCACATTGCGGTGGTGCTGGTCGGCGTGCTGATGATGATGTACGTCCTGTTCGGCGGCATGCTGGCGACCAC  <  NC_000913/4284427‑4284289
                                                                                                                                                                                                                                                                                                         
TTACTCGCTGGGCTTCCTGGTGGGCTGGCCGATCATTTTGTTCCTGATTGCCGAACGTCTGCGTAACCTGGGGCGCTACACCTTTGCCGATGTGGCCTCTTACCGTCTGAAACAAGGGCCGATTCGTATTCTTTCGGCCTGTGGTTCTCT                                                                                                                                                     <  2:289779/150‑1
 TACTCGCTGGGCTTCCTGGTGGGCTGGCCGATCATTTTGTTCCTGATTGCCGAACGTCTGCGTAACCTGGGGCGCTACACCTTTGCCGATGTGGCCTCTTACCGTCTGAAACAAGGGCCGATTCGTATTCTTTCGGCCTGTGGTTCTCTGG                                                                                                                                                   >  1:350641/1‑151
        TGGGCTTCCTGGTGGGCTGGCCGATCATTTTGTTCCTGATTGCCGAACGTCTGCGTAACCTGGGGCGCTACACCTTTGCCGATGTGGCCTCTTACCGTCTGAAACAAGGGCCGATTCGTATTCTTTCGGCCTGTGGTTCTCTGGTGGTGGC                                                                                                                                            >  2:416118/1‑151
              TCCTGGTGGGCTGGCCGATCATTTTGTTCCTGATTGCCGAACGTCTGCGTAACCTGGGGCGCTACACCTTTGCCGATGTGGCCTCTTACCGTCTGAAACAAGGGCCGATTCGTATTCTTTCGGCCTGTGGTTCTCTGGTGGTGGCGCTTT                                                                                                                                       >  1:129845/1‑150
                        CTGGCCGATCATTTTGTTCCTGATTGCCGAACGTCTGCGTAACCTGGGGCGCTACACCTTTGCCGATGTGGCCTCTTACCGTCTGAAACAAGGGCCGATTCGTATTCTTTCGGCCTGTGGTTCTCTGGTGGTGGCGCTTTACCTTATCGCC                                                                                                                            >  1:17963/1‑151
                         TGGCCGATCATTTTGTTCCTGATTGCCGAACGTCTGCGTAACCTGGGGCGCTACACCTTTGCCGATGTGGCCTCTTACCGTCTGAAACAAGGGCCGATTCGTATTCTTTCGGCCTGTGGTTCTCTGGTGGTGGCGCTTTACCTTATCGCCC                                                                                                                           >  2:271261/1‑151
                                 CATTTTGTTCCTGATTGCCGAACGTCTGCGTAACCTGGGGCGCTACACCTTTGCCGATGTGGCCTCTTACCGTCTGAAACAAGGGCCGATTCGTATTCTTTCGGCCTGTGGTTCTCTGGTGGTGGCGCTTTACCTTATCGCCCAGATGGTG                                                                                                                   >  1:538938/1‑151
                                        TTCCTGATTGCCGAACGTCTGCGTAACCTGGGGCGCTACACCTTTGCCGATGTGGCCTCTTACCGTCTGAAACAAGGGCCGATTCGTATTCTTTCGGCCTGTGGTTCTCTGGTGGTGGCGCTTTACCTTATCGCCCAGATGGTGGGCGC                                                                                                              >  1:371210/1‑149
                                               TTGCCGAACGTCTGCGTAACCTGGGGCGCTACACCTTTGCCGATGTGGCCTCTTACCGTCTGAAACAAGGGCCGATTCGTATTCTTTCGGCCTGTGGTTCTCTGGTGGTGGCGCTTTACCTTATCGCCCAGATGGTGGGCGCAGGTAAACT                                                                                                     >  2:623568/1‑151
                                                  CCGAACGTCTGCGTAACCTGGGGCGCTACACCTTTGCCGATGTGGCCTCTTACCGTCTGAAACAAGGGCCGATTCGTATTCTTTCGGCCTGTGGTTCTCTGGTGGTGGCGCTTTACCTTATCGCCCAGATGGTGGGCGCAGGTAAACTGAT                                                                                                  >  1:96564/1‑151
                                                  CCGAACGTCTGCGTAACCTGGGGCGCTACACCTTTGCCGATGTGGCCTCTTACCGTCTGAAACAAGGGCCGATTCGTATTCTTTCGGCCTGTGGTTCTCTGGTGGTGGCGCTTTACCTTATCGCCCAGATGGTGGGCGCAGGTAAACTGAT                                                                                                  >  1:236520/1‑151
                                                          CTGCGTAACCTGGGGCGCTACACCTTTGCCGATGTGGCCTCTTACCGTCTGAAACAAGCGCCGATTCGTATTCTTTCGGCCTGTGGTTCTCTGGTGGTGGCGCTTTACCTTATCGCCCAGATGGTGGGCGCAGGTAAACTGATCGAGC                                                                                             >  1:460774/1‑148
                                                            GCGTAACCTGGGGCGCTACACCTTTGCCGATGTGGCCTCTTACCGTCTGAAACAAGGGCCGATTCGTATTCTTTCGGCCTGTGGTTCTCTGGTGGTGGCGCTTTACCTTATCGCCCAGATGGTGGGCGCAGGTAAACTGATCGAGCTG                                                                                           <  1:207058/148‑1
                                                                AACCTGGGGCGCTACACCTTTGCCGATGTGGCCTCTTACCGTCTGAAACAAGGGCCGATTCGTATTCTTTCGGCCTGTGGTTCTCTGGTGGTGGCGCTTTACCTTATCGCCCAGATGGTGGGCGCAGGTAAACTGATCGAGCTGCTGTTTG                                                                                    <  2:75418/151‑1
                                                                AACCTGGGGCGCTACACCTTTGCCGATGTGGCCTCTTACCGTCTGAAACAAGGGCCGATTCGTATTCTTTCGGCCTGTGGTTCTCTGGTGGTGGCGCTTTACCTTATCGCCCAGATGGTGGGCGCAGGTAAACTGATCGAGCTGCTGTTT                                                                                     <  1:245154/150‑1
                                                                 ACCTGGGGCGCTACACCTTTGCCGATGTGGCCTCTTACCGTCTGAAACAAGGGCCGATTCGTATTCTTTCGGCCTGTGGTTCTCTGGTGGTGGCGCTTTACCTTATCGCCCAGATGGTGGGCGCAGGTAAACTGATCGAGCTGCTGTTTGG                                                                                   >  1:335616/1‑151
                                                                      GGGCGCTACACCTTTGCCGATGTGGCCTCTTACCGTCTGAAACAAGGGCCGATTCGTATTCTTTCGGCCTGTGGTTCTCTGGTGGTGGCGCTTTACCTTATCGCCCAGATGGTGGGCGCAGGTAAACTGATCGAGCTGCTGTTTGGCCTTA                                                                              <  1:288284/151‑1
                                                                        GCGCTACACCTTTGCCGATGTGGCCTCTTACCGTCTGAAACAAGGGCCGATTCGTATTCTTTCGGCCTGTGGTTCTCTGGTGGTGGCGCTTTACCTTATCGCCCAGATGGTGGGCGCAGGTAAACTGATCGAGCTGCTGTTTGGCCTTAAC                                                                            <  2:86385/151‑1
                                                                            TACACCTTTGCCGATGTGGCCTCTTACCGTCTGAAACAAGGGCCGATTCGTATTCTTTCGGCCTGTGGTTCTCTGGTGGTGGCGCTTTACCTTATCGCCCAGATGGGGGGCGCAGGTAAACTGATCGAGCTGCTGTTTGGCCTTAACTATC                                                                        >  2:393244/1‑151
                                                                             ACACCTTTGCCGATGTGGCCTCTTACCGTCTGAAACAAGGGCCGATTCGTATTCTTTCGGCCTGTGGTTCTCTGGTGGTGGCGCTTTACCTTATCGCCCAGATGGTGGGCGCAGGTAAACTGATCGAGCTGCTGTTTGGCCTTAACTAT                                                                         <  1:560570/149‑1
                                                                              CACCTTTGCCGATGTGGCCTCTTACCGTCTGAAACAAGGGCCGATTCGTATTCTTTCGGCCTGTGGTTCTCTGGTGGTGGCGCTTTACCTTATCGCCCAGATGGTGGGCGCAGGTAAACTGATCGAGCTGCTGTTTGGCCTTAACTATCAC                                                                      <  2:253019/151‑1
                                                                                CCTTTGCCGATGTGGCCTCTTACCGTCTGAAACAAGGGCCGATTCGTATTCTTTCGGCCTGTGGTTCTCTGGTGGTGGCGCTTTACCTTATCGCCCAGATGGTGGGCGCAGGTAAACTGATCGAGCTGCTGTTTGGCCTTAACTATCACA                                                                     <  1:299092/150‑1
                                                                                  TTTGCCGATGTGGCCTCTTACCGTCTGAAACAAGGGCCGATTCGTATTCTTTCGGCCTGTGGTTCTCTGGTGGTGGCGCTTTACCTTATCGCCCAGATGGTGGGCGCAGGTAAACTAATCGAGCTGCTGTTTGGCCTTAACTATCACATTG                                                                  >  2:367690/1‑151
                                                                                   TTGCCGATGTGGCCTCTTACCGTCTGAAACAAGGGCCGATTCGTATTCTTTCGGCCTGTGGTTCTCTGGTGGTGGCGCTTTACCTTATCGCCCAGATGGTGGGCGCAGGTAAACTGATCGAGCTGCTGTTTGGCCTTAACTATCACATT                                                                   <  2:324085/149‑1
                                                                                     GCCGATGTGGCCTCTTACCGTCTGAAACAAGGGCCGATTCGTATTCTTTCGGCCTGTGGTTCTCTGGTGGTGGCGCTTTACCTTATCGCCCAGATGGTGGGCGCAGGTAAACTGATCGAGCTGCTGTTTGGCCTTAACTATCACATTGCGG                                                               >  2:399133/1‑151
                                                                                       CGATGTGGCCTCTTACCGTCTGAAACAAGGGCCGATTCGTATTCTTTCGGCCTGTGGTTCTCTGGTGGTGGCGCTTTACCTTATCGCCCAGATGGTGGGCGCAGGTAAACTGATCGAGCTGCTGTTTGGCCTTAACTATCACATTGCGGTG                                                             <  2:248555/151‑1
                                                                                        GATGTGGCCTCTTACCGTCTGAAACAAGGGCCGATTCGTATTCTTTCGGCCTGTGGTTCTCTGGTGGTGGCGCTTTACCTTATCGCCCAGATGGTGGGCGCAGGTAAACTGATCGAGCTGCTGTTTGGCCTTAACTATCACATTGCGGTGG                                                            >  2:569775/1‑151
                                                                                             GGCCTCTTACCGTCTGAAACAAGGGCCGATTCGTATTCTTTCGGCCTGTGGTTCTCTGGTGGTGGCGCTTTACCTTATCGCCCAGATGGTGGGCGCAGGTAAACTGATCGAGCTGCTGTTTGGCCTTAACTATCACATTGCGGTGGTGCTG                                                       >  1:337024/1‑151
                                                                                                     ACCGTCTGAAACAAGGGCCGATTCGTATTCTTTCGGCCTGTGGTTCTCTGGTGGTGGCGCTTTACCTTATCGCCCAGATGGTGGGCGCAGGTAAACTGATCGAGCTGCTGTTTGGCCTTACCTATCACATTGCGGTGGTGTTGGTCG                                                   >  2:217028/1‑147
                                                                                                     ACCGTCTGAAACAAGGGCCGATTCGTATTCTTTCGGCCTGTGGTTCTCTGGTGGTGGCGCTTTACCTTATCGCCCAGATGGTGGGCGCAGGTAAACTGATCGAGCTGCTGTTTGGCCTTAACTATCACATTGCGGTGGTGCTGGTCGGCG                                                <  1:572440/150‑1
                                                                                                      CCGTCTGAAACAAGGGCCGATTCGTATTCTTTCGGCCTGTGGTTCTCTGGTGGTGGCGCTTTACCTTATCGCCCAGATGGTGGGCGCAGGTAAACTGATCGAGCTGCTGTTTGGCCTTAACTATCACATTGCGGTGGTGCTGGTCGGCGTG                                              <  2:225995/151‑1
                                                                                                               ACAAGGGCCGATTCGTATTCTTTCGGCCTGTGGTTCTCTGGTGGTGGCGCTTTACCTTATCGCCCAGATGGTGGGCGCAGGTAAACTGATCGAGCTGCTGTTTGGCCTTAACTATCACATTGCGGTGGTGCTGGTCGGCGTGCTGATGATG                                     <  2:388154/151‑1
                                                                                                                CAAGGGCCGATTCGTATTCTTTCGGCCTGTGGTTCTCTGGTGGTGGCGCTTTACCTTATCGCCCAGATGGTGGGCGCAGGTAAACTGATCGAGCTGCTGTTTGGCCTTAACTATCACATTGCGGTGGTGCTGGTCGGCGTGCTGATGATG                                     >  1:117564/1‑150
                                                                                                                    GGCCGATTCGTATTCTTTCGGCCTGTGGTTCTCTGGTGGTGGCGCTTTACCTTATCGCCCAGATGGTGGGCGCAGGTAAACTGATCGAGCTGCTGTTTGGCCTTAACTATCACATTGCGGTGGTGCTGGTCGGCGTGCTGATGATGATGTA                                <  1:395510/151‑1
                                                                                                                         ATTCGTATTCTTTCGGCCTGTGGTTCTCTGGTGGTGGCGCTTTACCTTATCGCCCAGATGGTGGGCGCAGGTAAACTGATCGAGCTGCTGTTTGGCCTTAACTATCACATTGCGGTGGTGCTGGTCGGCGTGCTGATGATGATGTACGTCC                           >  2:458628/1‑151
                                                                                                                            CGTATTCTTTCGGCCTGTGGTTCTCTGGTGGTGGCGCTTTACCTTATCGCCCAGATGGTGGGCGCAGGTAAACTGATCGAGCTGCTGTTTGGCCTTAACTATCACATTGCGGTGGTGCTGGTCGGCGTGCTGATGATGATGTACGTCCTGT                        >  2:330347/1‑151
                                                                                                                            CGTATTCTTTCGGCCTGTGGTTCTCTGGTGGTGGCGCTTTACCTTATCGCCCAGATGGTGGGCGCAGGTAAACTGATCGAGCTGCTGTTTGGCCTTAACTATCACATTGCGGTGGTGCTGGTCGGCGTGCTGATGATGATGTACGTCCTGT                        >  1:553362/1‑151
                                                                                                                                       GGCCTGTGGTTCTCTGGTGGTGGCGCTTTACCTTATCGCCCAGATGGTGGGCGCAGGTAAACTGATCGAGCTGCTGTTTGGCCTTAACTATCACATTGCGGTGGTGCTGGTCGGCGTGCTGATGATGATGTACGTCCTGTTCGGCGGCATG             >  1:422397/1‑151
                                                                                                                                         CCTGTGGTTCTCTGGTGGTGGCGCTTTACCTTATCGCCCAGATGGTGGGCGCAGGTAAACTGATCGAGCTGCTGTTTGGCCTTAACTATCACATTGCGGTGGTGCTGGTCGGCGTGCTGATGATGATGTACGTCCTGTTCGGCGGCATGCT           <  2:335609/151‑1
                                                                                                                                            GTGGTTCTCTGGTGGTGGCGCTTTACCTTATCGCCCAGATGGTGGGCGCAGGTAAACTGATCGAGCTGCTGTTTGGCCTTAACTATCACATTGCGGTGGTGCTGGTCGGCGTGCTGATGATGATGTACGTCCTGTTCGGCGGCATGCTGGC        >  1:59882/1‑151
                                                                                                                                            GTGGTTCTCTGGTGGTGGCGCTTTACCTTATCGCCCAGATGGTGGGCGCAGGTAAACTGATCGAGCTGCTGTTTGGCCTTAACTATCACATTGCGGTGGTGCTGGTCGGCGTGCTGATGATGATGTACGTCCTGTTCGGCGGCATGCTG          <  2:142535/149‑1
                                                                                                                                            GTGGTTCTCTGGTGGTGGCGCTTTACCTTATCGCCCAGATGGTGGGCGCAGGTAAACTGATCGAGCTGCTGTTTGGCCTTAACTATCACATTGCGGTGGTGCTGGTCGGCGTGCTGATGATGATGTACGTCCTGTTCGGCGGCATGCTG          <  1:99385/149‑1
                                                                                                                                              GGTTCTCTGGTGGTGGCGCTTTACCTTATCGCCCAGATGGTGGGCGCAGGTAAACTGATCGAGCTGCTGTTTGGCCTTAACTATCACATTGCGGTGGTGCTGGTCGGCGTGCTGATGATGATGTACGTCCTGTTCGGCGGCATGCTGGCGA      <  2:129842/151‑1
                                                                                                                                                  CTCTGGTGGTGGCGCTTTACCTTATCGCCCAGATGGTGGGCGCAGGTAAACTGATCGAGCTGCTGTTTGGCCTTAACTATCACATTGCGGTGGTGCTGGTCGGCGTGCTGATGATGATGTACGTCCTGTTCGGCGGCATGCTGGCGACCAC  >  1:22385/1‑151
                                                                                                                                                                                                                                                                                                         
TTACTCGCTGGGCTTCCTGGTGGGCTGGCCGATCATTTTGTTCCTGATTGCCGAACGTCTGCGTAACCTGGGGCGCTACACCTTTGCCGATGTGGCCTCTTACCGTCTGAAACAAGGGCCGATTCGTATTCTTTCGGCCTGTGGTTCTCTGGTGGTGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/4284588‑4284431
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tggtggtggCGCTTTACCTTATCGCCCAGATGGTGGGCGCAGGTAAACTGATCGAGCTGCTGTTTGGCCTTAACTATCACATTGCGGTGGTGCTGGTCGGCGTGCTGATGATGATGTACGTCCTGTTCGGCGGCATGCTGGCGACCAC  <  NC_000913/4284427‑4284289

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.