Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091317,0000CT45.5% 37.2 / 20.6 44intergenic (‑40/‑489)mokC/nhaAregulatory protein for HokC, overlaps CDS of hokC/sodium‑proton antiporter
Reads supporting (aligned to +/- strand):  ref base C (18/6);  new base T (0/20);  total (18/26)
Fisher's exact test for biased strand distribution p-value = 1.57e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTGATACAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAATGCCTGAGACAGACAGCCTCAAGCACCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGAT  >  NC_000913/16853‑17146
                                                                                                                                                   |                                                                                                                                                   
ggTGATACAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCttt                                                                                                                                                  <  1:187062/151‑1 (MQ=255)
ggTGATACAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCttt                                                                                                                                                  >  1:378684/1‑151 (MQ=255)
   gATACAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTtc                                                                                                                                                <  2:392042/150‑1 (MQ=255)
     tACAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTctct                                                                                                                                             >  1:342280/1‑151 (MQ=255)
                        gACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAATg                                                                                                                          >  2:252427/1‑151 (MQ=255)
                         aCAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAATGc                                                                                                                         >  1:230056/1‑151 (MQ=255)
                                tcGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAATGCCTGagac                                                                                                                 >  1:543208/1‑151 (MQ=255)
                                   ccTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAATGCCTGagac                                                                                                                 >  1:515864/1‑148 (MQ=255)
                                     ttATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAATGCCTGagacagac                                                                                                             >  2:431819/1‑150 (MQ=255)
                                         gctgctTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAATGCCTGAGACAGACAGCCt                                                                                                        >  1:92060/1‑151 (MQ=255)
                                          ctgctTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAATGCCTGAGACAGACAGCCTc                                                                                                       >  2:348428/1‑151 (MQ=255)
                                                       ctctTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAATGCCTGAGACAGACAGCCTCAAGCACccgcc                                                                                            <  1:186663/149‑1 (MQ=255)
                                                             tcCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAATGCCTGAGACAGACAGCCTCAAGCACCCGCCGCtattat                                                                                    >  2:78120/1‑151 (MQ=255)
                                                                ttGCCCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGAATATGGGAGGCCTCGGGTTGATGAAAAAATATCCCTCGGGGTTTTTCTCTATCTCCCTTTCAGCAAATGCCTGAGACAGACAGCCTCAAGCCCCCGCCGCTATTatat                                                                                  <  2:543177/150‑1 (MQ=255)
                                                                 tgcccTTACGGTAAGAAAGAGGCACTCTACATTTGTTCAGAATATAGGGGGCCTGGGTTTGATGGAAAAATACCCCTGGGGCTTTTTCTCTACCTCCCTTTCAGCAAATCCCTGAGACAGACAGCCTCAAGCCCCCGCCGCTATTatat                                                                                  <  2:109382/146‑1 (MQ=255)
                                                                          ggTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAATGCCTGAGACAGACAGCCTCAAGCACCCGCCGCTATTATATCGCTCTCttt                                                                        >  2:286063/1‑150 (MQ=255)
                                                                          ggTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAATGCCTGAGACAGACAGCCTCAAGCACCCGCCGCTATTATATCGCTCTCttt                                                                        >  1:291632/1‑150 (MQ=255)
                                                                                   gggcccctctACATGTTTTCAGCATATGGGAGGCCTCGGGTTAATGGTAAAATATCCCTCGGGGTTTTTCTTTATCTCCCGTTCAGCTAATCCCTGAGACAGACACCCTCAAGCACCCGCCGCTATTATATCGCTCTCTTTAACCCAtttt                                                              <  2:147475/145‑1 (MQ=255)
                                                                                      gctctatACGTGTGTTCATCATATGGGGGGCCTGGGTTTGGTGGAAAAATATCACTCGGGTTTTTTCTTTATCCGCCGTTCACCAATGCCATGAGAAAAACAGCCTCAACCAGCCGCCGCTATTATATCGCTCTCTTTAACCCAtttt                                                              <  1:545926/142‑1 (MQ=255)
                                                                                         ctctACTTGTTCTAAACATATGGGAGGCCGGGGTTGGATGGAAAAATACCCCCCGGGCCTTCTTTCTACCTCCCGTTCAGCTAATCCCTGATAGAGACACCCCCGAGCGCCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGttt                                                          <  2:531490/149‑1 (MQ=255)
                                                                                             aCATGTGTTCAGCATATAGGGGGCCTGGGGTTGATGGAAAAATATCCCTGGGGTTTTTTCTTTATCTGCCTTTCAGCTAATGCCTGAGACAGACAGCCTCAACCACCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCg                                                     <  1:515457/150‑1 (MQ=255)
                                                                                                       aaaatatAGGGGCCCGGGGGTTGATGATAAAATACCCCTCGGGGTTTTTCTCTATCTGCCGTCCATCTAATGCTTGAGACAACCACCCTCAAGCACCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTa                                               <  1:545928/143‑1 (MQ=255)
                                                                                                       aGCATAAGGGGGGCCTGGGGTTGATTAAAAAATTTCTTTGGGGGCTTTTCTCTCCCCGCCGTTCCGCAAATCCTTGAGAAAGACACCCTCATTCCCCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATc                                            <  1:326446/149‑1 (MQ=255)
                                                                                                          atatAGGAGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAATGCCTGAGACAGACAGCCTCAAGCACCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATc                                            >  1:455171/1‑146 (MQ=255)
                                                                                                             tgggaggCCTGGGTTTGATGTAAAAATACCCCCCGGGTTTTTTCTCTATCTCCCGTTCAGCTAATCCCTGAGACAGACACCCTCAAGCACCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATc                                            <  2:455163/141‑1 (MQ=255)
                                                                                                               ggggcccgggggTTGATGGAAAAATATCACTGGGGTTTTTTCTCTATCTGCCTTACAGTTAATCCCGGAAAAAGACAGCCACAACCACCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAg                                      <  1:369826/138‑1 (MQ=255)
                                                                                                               ggggcccTGGGTTTGATGAAAAAATTCCCCGCGGGGTTTTTCTCTCTCTCCCGTTCACCTATTCCGTGAAACACACACCCTCAACCCCCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGaa                                       <  2:213372/141‑1 (MQ=255)
                                                                                                                  ggCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAATGCCTGAGACAGACAGCCTCAAGCACCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTc                               >  2:46893/1‑151 (MQ=255)
                                                                                                                        ggtttGATGAAAAAATACCCCGCGGGTTTTTTCTTTATCGCCCTTAAAGTAAATGCCTGAGACAGACCCCCTAAACCCCCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTc                               <  2:140066/142‑1 (MQ=255)
                                                                                                                        gggTTGATGGAAAAATATCCCTCGGGTTTTTTCTCTATCTCCCTTTCAGCAAATGCCTGAGAAAGACACCCTAAAGCCCCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCAttt                         <  2:72859/151‑1 (MQ=255)
                                                                                                                          gTTGATGGTAAAATATCACTCGGGACTTTTATCTATCTGCCGTTCAGCTACTGCCTGAGACAGTCAGCCTCAAGCAACCGCCGCTATTATATCGCTCTCCTTCACCCATTTTGTTTTATCGAGTCTAATTCTGAAGACGCCTCGTAt                           >  2:437256/1‑147 (MQ=255)
                                                                                                                              aTGGAAAAGTATCTCGCGGGTTTTTTCTCTTTCGGCCGTTCACCTACTGTCGGATAAAGCCAGCCTAAACCCCCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCAtttt                        <  2:560542/146‑1 (MQ=255)
                                                                                                                               tgaaaaaaTATCCCGGGGGTTTTTTCTCTATCTGCCTTTCAGCTAATGCCTGAGACAGACAGCCTCAAGCCCCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTtgt                     <  2:414040/144‑1 (MQ=255)
                                                                                                                                ggaaaaaTACCCCTCGGGGTTTTTCTCTTTCTCCCGTTCAGCTAATCCCTGAGACAGCCAGCCAAAACCCCCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTtgt                     <  1:181172/144‑1 (MQ=255)
                                                                                                                                   aaaaTCCCACGCGGGGTTTTTCTCTCTCTCCCTTTCATCAAATCCCTGAAACACACCGCCTCAAGCCCCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCAttttt                       <  2:32667/142‑1 (MQ=255)
                                                                                                                                   aaaaTACCCCTGGGGGTTTTTCTCTATCTGCCGTTCAGCTAATGCCGGAGACAGACACCCTAAAGCCCCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTgg                   <  1:363047/146‑1 (MQ=255)
                                                                                                                                      ataccccggggggTTTTTTTCTACCTGCCGTTCAGCTAATGCCTGAGACAGACAGCCTCAACCCCCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAAttt           <  1:443568/142‑1 (MQ=255)
                                                                                                                                       tatCACTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAATGCCTGAGACAGACAGCCTCAAGCACCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAAtttt          >  2:313518/1‑151 (MQ=255)
                                                                                                                                        atCACTCGGGTCTTTTCTCTACCTCCCTTTCAGCTAATGCCTGAGACAGACACCCTCAACCACCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAAtttt          <  2:251010/150‑1 (MQ=255)
                                                                                                                                        atCACTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAATGCCTGAGACAGACAGCCTCAAGCACCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAAttttt         >  1:148577/1‑151 (MQ=255)
                                                                                                                                          cccTCGGGTTTTTTCTCTATCTGCCTTTCATTTAATCCCTGAGACAGACACCCTCAAGCCCCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGTGTAAttattt        <  1:83393/148‑1 (MQ=255)
                                                                                                                                               ggggTTTTTCTCTATCTGCCGTTCAGCTAATGCCTGAGACAGACAGCCTCAAGCCCCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATg    <  1:348435/149‑1 (MQ=255)
                                                                                                                                               ggggTTTTTCTCTACCTGCCTTTCAGCTAATGCTTGAGAAAGACACCCTCAAGCCCCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGAt  <  2:90346/151‑1 (MQ=255)
                                                                                                                                               ggggCTTTTCTCTATCTGCCGTTCAGCTAATGCCTGAGACAGACAGCCTCAAGCACCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGAt  >  2:159602/1‑151 (MQ=255)
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GGTGATACAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGCTCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTATCTGCCGTTCAGCTAATGCCTGAGACAGACAGCCTCAAGCACCCGCCGCTATTATATCGCTCTCTTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGAT  >  NC_000913/16853‑17146

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: