Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913941,7130TG51.6% 30.5 / 50.8 31V252G (GTG→GGG) dmsAdimethyl sulfoxide reductase, anaerobic, subunit A
Reads supporting (aligned to +/- strand):  ref base T (2/13);  new base G (16/0);  total (18/13)
Fisher's exact test for biased strand distribution p-value = 5.09e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.89e-06

TACTCCTCCGCGCAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGATGCCGCACTGGTTAACG  >  NC_000913/941568‑941853
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tactCCTCCGCGCAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGGGGGGGGACtt                                                                                                                                         >  2:501904/1‑151 (MQ=255)
        cgcgCAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCtt                                                                                                                                 <  1:510952/151‑1 (MQ=255)
        cgcgCAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGGGGGGGGGCTTTCTATCtt                                                                                                                                 >  2:416412/1‑151 (MQ=255)
                 tGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGCCGGCGTGGTTACTTaacacttttgataag                                                                                                                          >  2:623730/1‑135 (MQ=255)
                   cGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACg                                                                                                                      <  1:139527/151‑1 (MQ=255)
                            tGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTACTATCTTTAAAAAGCACGCCAGaaa                                                                                                               >  1:158183/1‑149 (MQ=255)
                             gAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATc                                                                                                             <  2:498994/150‑1 (MQ=255)
                                   taCCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTACTATCTTTAACAGGCACGCCAGAAATATAATTcc                                                                                                      >  1:115937/1‑151 (MQ=255)
                                    aCCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTACTATCTTTAAAAGGCACCCCCGAAAACTAATGccc                                                                                                     >  1:379470/1‑151 (MQ=255)
                                      cTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGGAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGGGGGGGGGCTTTCTATCTTGTAAAAGCACGCCAGAAAACTAATGccccc                                                                                                   >  2:535835/1‑149 (MQ=255)
                                                  ggCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGatcatcatc                                                                                       <  2:519404/151‑1 (MQ=255)
                                                    cAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGa                                                                                     <  2:366341/151‑1 (MQ=255)
                                                     aGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTAATATCTTTAAAAAGCACGCCCGAAATCTAATTCCCGCATGaacatcat                                                                                        >  2:596874/1‑147 (MQ=255)
                                                        tGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGGGGGGGGGCTTACTATCTTTAACAAGCACGCCAGAAAACTAAAGCCCGCATGATCATCATCGATcc                                                                                  >  2:571376/1‑150 (MQ=255)
                                                             aCAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGa                                                                                               <  1:297399/132‑1 (MQ=255)
                                                                 cccGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGGTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTAATATCTTTAACAAGACCGCCAGAAATCTAATGGCCGCATGAACGTCCTCggatcgcgcca                                                                            >  1:82953/1‑137 (MQ=255)
                                                                  ccGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCg                                                                       <  1:212040/151‑1 (MQ=255)
                                                                             cGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTAATATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCCTGATCATCATCGATCCGCGCTATACCGACACCGGGGcc                                                            >  1:607867/1‑151 (MQ=255)
                                                                              gAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTACTATCTTGAAAAAGCACGCCAGAAAACTAATGCCCGCATGATCATCATCGATCCCCGCCATACCCACACCGGGGcc                                                            >  2:324713/1‑150 (MQ=255)
                                                                               aaaaCAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCgg                                                          <  1:554738/151‑1 (MQ=255)
                                                                               aaaaCAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCgg                                                          <  1:99264/151‑1 (MQ=255)
                                                                                    aGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTACTATCTTTAACAAGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGgcg                                                       >  1:551247/1‑149 (MQ=255)
                                                                                      tAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTACTATCTTGAAAAAGCAAGCCCGAAATCTAAAGCCCGCCTGATCATCATCGATCCGCGCCATACCGACACCCGGGGCggg                                                         >  2:144921/1‑145 (MQ=255)
                                                                                      tAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTACTATCTTGAACAGGCACGCCCGAAAACTAAAGCCCCCATGATCATCATCGATCCGCGCTATACCGACACCGGGGCCGGGcgcg                                                     >  1:140623/1‑149 (MQ=255)
                                                                                                  gCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGGCTTTCTATCTTTAACAGGCACCCCAGAAATCTAATGCCCCCATGATCCTCATCTATCCGCGCTATACCCACACCCGTGCCGGGCGCGAAGATGAGTggggc                                       >  1:297097/1‑148 (MQ=255)
                                                                                                        tGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTAt                                  <  1:261052/150‑1 (MQ=255)
                                                                                                             ataaCCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTcc                            <  1:416419/151‑1 (MQ=255)
                                                                                                                  ccTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTACTATCTTGAAAAGGCACGCCAGAAATCTAAAGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGGGCCGGGCGCGAAGATGAGGGGATCCCTATTCGTCCggg                         >  1:219746/1‑149 (MQ=255)
                                                                                                                   cTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTACTATTTTTAACAGGGCCGCCAGAAAACTAAAGCCCGCCAGATCATCATCGGACCGCGGTATAACCACACCGGTGGCGGGCGCGGAGATGAGGGGGTCCCTTTTCGTCCgggga                       >  2:573470/1‑148 (MQ=255)
                                                                                                                                       ggCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGATGCCGCACTGGTTAACg  <  2:247371/151‑1 (MQ=255)
                                                                                                                                       ggCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGATGCCGCACTGGTTAACg  <  1:324720/151‑1 (MQ=255)
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TACTCCTCCGCGCAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGATGCCGCACTGGTTAACG  >  NC_000913/941568‑941853

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑