Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,270,4550GT50.0% 22.8 / 13.0 26intergenic (+853/+170)yhaC/garKpentapetide repeats‑related protein/glycerate kinase I
Reads supporting (aligned to +/- strand):  ref base G (2/11);  new base T (13/0);  total (15/11)
Fisher's exact test for biased strand distribution p-value = 2.02e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGGCCGTACCTTATGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGGTTTCCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGGCGCGCAGT  >  NC_000913/3270313‑3270602
                                                                                                                                              |                                                                                                                                                     
cGGGCCGTACCTTATGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTCCCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCCCTCTGt                                                                                                                                               <  1:61303/151‑1 (MQ=255)
  ggCCGTACCTTATGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCTCtttt                                                                                                                                              >  2:221228/1‑150 (MQ=255)
            tATGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCg                                                                                                                                    <  1:295280/150‑1 (MQ=255)
            tATGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTGTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTc                                                                                                                                     <  2:325474/149‑1 (MQ=255)
             aTGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGt                                                                                                                                  <  2:406743/150‑1 (MQ=255)
               gAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTggg                                                                                                                              <  2:21986/151‑1 (MQ=255)
                 aCCCCTCTTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTTTCTCTGTTGTACTCGGTGTggg                                                                                                                              >  2:499510/1‑149 (MQ=255)
                          tGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCTCTTTTTCACTGGTCGGGGGTTTtccccccc                                                                                                                   >  1:176603/1‑150 (MQ=255)
                           ggCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGGTTTCCCCCCCa                                                                                                                   <  1:567854/150‑1 (MQ=255)
                           ggCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGGTTTCCCCCCCa                                                                                                                   <  2:351020/150‑1 (MQ=255)
                            gCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCTCTTTTTCCCTTGGCGTGGGGTTTccccccc                                                                                                                    >  2:560329/1‑149 (MQ=255)
                                tGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGGGTTTccccccccgcggt                                                                                                              >  1:524164/1‑145 (MQ=255)
                                                               tGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCTCTTTTTCACTGGTCGTGGGGTTTCCCCCCCGGCGGTACCTGGCACCCCGCCCCATGGAGccc                                                                                   >  2:364519/1‑146 (MQ=255)
                                                               tGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCTCTTTTGCACTTGTCGTGGGTTTCCCCCCCCCGCGTTACCTGGGACCCTGCCCTATGGAGccc                                                                                   >  1:516785/1‑146 (MQ=255)
                                                                         cgcgcgGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGGTTTCCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTcctcccct                                                                    <  2:480929/151‑1 (MQ=255)
                                                                                   gcTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCTCTTTTGCACTGGTCGTGGGGTTCCCCCCCCGGCGTTTCCTGGCACCCTGCCCCATGGAGCCCGGGCTTTCCTCCCCCCCGccc                                                              >  2:390213/1‑147 (MQ=255)
                                                                                      ctTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCTCTGTTGCACTTGGCGTGGGTTTTCCCCCCCCGCGGTACCTGGCACCCTTCCCTCTGGAGCCCGGGCTTTTCCCCCCCCCCCCCGTCTccc                                                       >  1:70671/1‑151 (MQ=255)
                                                                                          ccGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTTTCTCTGTTGTCCTGGGCCTTGGTTTTCCCCCCATGCCTTTCCCTGCCCCCCTCCCCCTTGCGCCCCGGCTTTCCTcccccccccccccccccccccc                                                   >  2:390436/1‑149 (MQ=255)
                                                                                           cGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGGTTTTCCTCTTTTTCGCCGTGCGGGGGGGTCCCCCCCCACCGTTATCCGGCACCCTGCCCCATTGCGGCCGCACTTTCCTCCCCCCCCCCCCTCcccccc                                                     >  2:260587/1‑148 (MQ=255)
                                                                                            gCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGGTTTCCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAag                                                 <  1:517115/151‑1 (MQ=255)
                                                                                             cACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGGTTTCCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAaga                                                <  1:206956/151‑1 (MQ=255)
                                                                                              aCCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTTTCTCTCTTGTACTTGTCGAGGGGTTCCCCCCCCCAGCGGTACCCGGGACCCCGCCCTATTGGGGCCGGGCTTTTCTCCCCCCCCCCCCTCCtcccc                                                     >  1:302374/1‑146 (MQ=255)
                                                                                                           tACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTTTTTCTGTTGCACTGGGCGTGGGGTTCCCCCCCCCGCGGTTCCCTGCACCCTGCCCTATTGGGCCCGGACTTTCCTCCCCCCCCCCCGTCTCCCCCCAAGAGGGCGACGACGaa                                   >  1:111239/1‑150 (MQ=255)
                                                                                                                        ttGCGCGGGCCATCGGCGGTTTTCTCTCTTTTGCCCCGGTCGTGGGGTTCCCCCCCCGGCGGTACCTGGCACCCCGCCCCATGGGGCCCGGGCCTTTCCCCCCCCCCCCCCTCTCCCCCCCAGAGGGCGACCACCAAacggcggccctc                       >  2:121953/1‑143 (MQ=255)
                                                                                                                                  cATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGGTTTCCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCg           <  2:583393/151‑1 (MQ=255)
                                                                                                                                           tttGCTCTCTGTTGCACTGGTCGTGGGTTTCCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGGCGCGCAGt  >  2:579854/1‑151 (MQ=255)
                                                                                                                                              |                                                                                                                                                     
CGGGCCGTACCTTATGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGGTTTCCCCCCCAGGCGTTACCTGGCACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGGCGCGCAGT  >  NC_000913/3270313‑3270602

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: