Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,467,446	A→G	K301R (AAG→AGG)	intS →	CPS‑53 (KpLE1) prophage; putative prophage CPS‑53 integrase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,467,446	0	A	G	100.0%	153.1 / NA	41	K301R (AAG→AGG)	intS	CPS‑53 (KpLE1) prophage; putative prophage CPS‑53 integrase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/27);  total (14/27)

ACCATCGACGCCAGTGTGATGAAAGGACGCAAAATTCATGTGGTTCCTATGTCAGACCAGGTAGTTGAACTTCTCACTACGCTAAGCTCCATCACCAAACCAGTCTCAGAGTTTGTTTTTGCCGGGCGCAACGATAAGAAGAAGCCAATCTGCGAGAACGCGGTACTGCTTGTGATCAAACAAATCGGCTATGAGGGTCTGGAAAGCGGTCACGGATTCAGGCATGAATTCAGCACGATTATGAACGAGCACGAATGGCCTGCTGACGCTATTGAAGTGCAACTG  >  NC_000913/2467304‑2467588                                                                                                                                               |                                                                                                                                               aCCATCGACGCCAGTGTGATGAAAGGACGCAAAATTCATGTGGTTCCTATGTCAGACCAGGTAGTTGAACTTCTCACTACGCTAAGCTCCATCACCAAACCAGTCTCAGAGTTTGTTTTTGCCGGGCGCAACGATAAGAAGAGGCCAATCt                                                                                                                                        <  1:474182/151‑1 (MQ=255)  ccATCGACGCCAGTGTGATGAAAGGACGCAAAATTCATGTGGTTCCTATGTCAGACCAGGTAGTTGAACTTCTCACTACGCTAAGCTCCATCACCAAACCAGTCTCAGAGTTTGTTTTTGCCGGGCGCAACGATAAGAAGAGGCCAATCTg                                                                                                                                       <  1:353896/151‑1 (MQ=255)    aTCGACGCCAGTGTGATGAAAGGACGCAAAATTCATGTGGTTCCTATGTCAGACCAGGTAGTTGAACTTCTCACTACGCTAAGCTCCATCACCAAACCAGTCTCAGAGTTTGTTTTTGCCGGGCGCAACGATAAGAAGAGGCCAATCTGCg                                                                                                                                     >  2:32871/1‑151 (MQ=255)         cGCCAGTGTGATGAAAGGACGCAAAATTCATGTGGTTCCTATGTCAGACCAGGTAGTTGAACTTCTCACTACGCTAAGCTCCATCACCAAACCAGTCTCAGAGTTTGTTTTTGCCGGGCGCAACGATAAGAAGAGGCCAATCTGCGAGAAc                                                                                                                                <  2:331393/151‑1 (MQ=255)         cGCCAGTGTGATGAAAGGACGCAAAATTCATGTGGTTCCTATGTCAGACCAGGTAGTTGAACTTCTCACTACGCTAAGCTCCATCACCAAACCAGTCTCAGAGTTTGTTTTTGCCGGGCGCAACGATAAGAAGAGGCCAATCTGCGAGAAc                                                                                                                                <  1:427638/151‑1 (MQ=255)                gtgATGAAAGGACGCAAAATTCATGTGGTTCCTATGTCAGACCAGGTAGTTGAACTTCTCACTACGCTAAGCTCCATCACCAAACCAGTCTCAGAGTTTGTTTTTGCCGGGCGCAACGATAAGAAGAGGCCAATCTGCGAGAACGCGGTAc                                                                                                                         >  2:526578/1‑151 (MQ=255)                        aGGACGCAAAATTCATGTGGTTCCTATGTCAGACCAGGTAGTTGAACTTCTCACTACGCTAAGCTCCATCACCAAACCAGTCTCAGAGTTTGTTTTTGCCGGGCGCAACGATAAGAAGAGGCCAATCTGCGAGAACGCGGTACTGCTtgtg                                                                                                                 <  2:123469/151‑1 (MQ=255)                          gACGCAAAATTCATGTGGTTCCTATGTCAGACCAGGTAGTTGAACTTCTCACTACGCTAAGCTCCATCACCAAACCAGTCTCAGAGTTTGTTTTTGCCGGGCGCAACGATAAGAAGAGGCCAATCTGCGAGAACGCGGTACTGCTTGTGAt                                                                                                               >  1:253146/1‑151 (MQ=255)                               aaaaTTCATGTGGTTCCTATGTCAGACCAGGTAGTTGAACTTCTCACTACGCTAAGCTCCATCACCAAACCAGTCTCAGAGTTTGTTTTTGCCGGGCGCAACGATAAGAAGAGGCCAATCTGCGAGAACGCGGTACTGCTTGTGATcaaa                                                                                                           <  2:617418/150‑1 (MQ=255)                                  aTTCATGTGGTTCCTATGTCAGACCAGGTAGTTGAACTTCTCACTACGCTAAGCTCCATCACCAAACCAGTCTCAGAGTTTGTTTTTGCCGGGCGCAACGATAAGAAGAGGCCAATCTGCGAGAACGCGGTACTGCTTGTGATcaaacaa                                                                                                        >  2:512921/1‑150 (MQ=255)                                     cATGTGGTTCCTATGTCAGACCAGGTAGTTGAACTTCTCACTACGCTAAGCTCCATCACCAAACCAGTCTCAGAGTTTGTTTTTGCCGGGCGCAACGATAAGAAGAGGCCAATCTGCGAGAACGCGGTACTGCTTGTGATCAAACAAATc                                                                                                     <  1:153689/150‑1 (MQ=255)                                          ggTTCCTATGTCAGACCAGGTAGTTGAACTTCTCACTACGCTAAGCTCCATCACCAAACCAGTCTCAGAGTTTGTTTTTGCCGGGCGCAACGATAAGAAGAGGCCAATCTGCGAGAACGCGGTACTGCTTGTGATCAAACAAATCGGCTa                                                                                                <  1:513381/150‑1 (MQ=255)                                          ggTTCCTATGTCAGACCAGGTAGTTGAACTTCTCACTACGCTAAGCTCCATCACCAAACCAGTCTCAGAGTTTGTTTTTGCCGGGCGCAACGATAAGAAGAGGCCAATCTGCGAGAACGCGGTACTGCTTGTGATCAAACAAATCGGCTa                                                                                                <  2:598991/150‑1 (MQ=255)                                           gTTCCTATGTCAGACCAGGTAGTTGAACTTCTCACTACGCTAAGCTCCATCACCAAACCAGTCTCAGAGTTTGTTTTTGCCGGGCGCAACGATAAGAAGAGGCCAATCTGCGAGAACGCGGTACTGCTTGTGATCAAACAAATCGGCTATg                                                                                              <  2:632086/151‑1 (MQ=255)                                           gTTCCTATGTCAGACCAGGTAGTTGAACTTCTCACTACGCTAAGCTCCATCACCAAACCAGTCTCAGAGTTTGTTTTTGCCGGGCGCAACGATAAGAAGAGGCCAATCTGCGAGAACGCGGTACTGCTTGTGATCAAACAAATCGGCTATg                                                                                              >  2:334311/1‑151 (MQ=255)                                             tCCTATGTCAGACCAGGTAGTTGAACTTCTCACTACGCTAAGCTCCATCACCAAACCAGTCTCAGAGTTTGTTTTTGCCGGGCGCAACGATAAGAAGAGGCCAATCTGCGAGAACGCGGTACTGCTTGTGATCAAACAAATCGGCTATg                                                                                              >  2:352472/1‑149 (MQ=255)                                                   gTCAGACCAGGTAGTTGAACTTCTCACTACGCTAAGCTCCATCACCAAACCAGTCTCAGAGTTTGTTTTTGCCGGGCGCAACGATAAGAAGAGGCCAATCTGCGAGAACGCGGTACTGCTTGTGATCAAACAAATCGGCTATGAGGGTCTg                                                                                      <  1:512020/151‑1 (MQ=255)                                                        aCCAGGTAGTTGAACTTCTCACTACGCTAAGCTCCATCACCAAACCAGTCTCAGAGTTTGTTTTTGCCGGGCGCAACGATAAGAAGAGGCCAATCTGCGAGAACGCGGTACTGCTTGTGATCAAACAAATCGGCTATGAGGGTCTGGAAAg                                                                                 <  2:239779/151‑1 (MQ=255)                                                                gTTGAACTTCTCACTACGCTAAGCTCCATCACCAAACCAGTCTCAGAGTTTGTTTTTGCCGGGCGCAACGATAAGAAGAGGCCAATCTGCGAGAACGCGGTACTGCTTGTGATCAAACAAATCGGCTATGAGGGTCTGGAAAGCGGTCACg                                                                         <  2:78251/151‑1 (MQ=255)                                                                        tctcACTACGCTAAGCTCCATCACCAAACCAGTCTCAGAGTTTGTTTTTGCCGGGCGCAACGATAAGAAGAGGCCAATCTGCGAGAACGCGGTACTGCTTGTGATCAAACAAATCGGCTATGAGGGTCTGGAAAGCGGTCACGGATTCa                                                                   <  2:608232/149‑1 (MQ=255)                                                                        tctcACTACGCTAAGCTCCATCACCAAACCAGTCTCAGAGTTTGTTTTTGCCGGGCGCAACGATAAGAAGAGGCCAATCTGCGAGAACGCGGTACTGCTTGTGATCAAACAAATCGGCTATGAGGGTCTGGAAAGCGGTCACGGATTCa                                                                   <  2:123094/149‑1 (MQ=255)                                                                                 gCTAAGCTCCATCACCAAACCAGTCTCAGAGTTTGTTTTTGCCGGGCGCAACGATAAGAAGAGGCCAATCTGCGAGAACGCGGTACTGCTTGTGATCAAACAAATCGGCTATGAGGGTCTGGAAAGCGGTCACGGATTCAGGCATGAATTc                                                        <  1:362100/151‑1 (MQ=255)                                                                                       cTCCATCACCAAACCAGTCTCAGAGTTTGTTTTTGCCGGGCGCAACGATAAGAAGAGGCCAATCTGCGAGAACGCGGTACTGCTTGTGATCAAACAAATCGGCTATGAGGGTCTGGAAAGCGGTCACGGATTCAGGCATGAATTCAGCAc                                                   <  2:223691/150‑1 (MQ=255)                                                                                        tCCATCACCAAACCAGTCTCAGAGTTTGTTTTTGCCGGGCGCAACGATAAGAAGAGGCCAATCTGCGAGAACGCGGTACTGCTTGTGATCAAACAAATCGGCTATGAGGGTCTGGAAAGCGGTCACGGATTCAGGCATGAATTCAGCACg                                                  >  1:356490/1‑150 (MQ=255)                                                                                         ccATCACCAAACCAGTCTCAGAGTTTGTTTTTGCCGGGCGCAACGATAAGAAGAGGCCAATCTGCGAGAACGCGGTACTGCTTGTGATCAAACAAATCGGCTATGAGGGTCTGGAAAGCGGTCACGGATTCAGGCATGAATTCAGCAAGAt                                                <  2:216034/151‑1 (MQ=255)                                                                                                      aGTCTCAGAGTTTGTTTTTGCCGGGCGCAACGATAAGAAGAGGCCAATCTGCGAGAACGCGGTACTGCTTGTGATCAAACAAATCGGCTATGAGGGTCTGGAAAGCGGTCACGGATTCAGGCATGAATTCAGCACGATTATGAACGAGCAc                                   <  2:38493/151‑1 (MQ=255)                                                                                                       gTCTCAGAGTTTGTTTTTGCCGGGCGCAACGATAAGAAGAGGCCAATCTGCGAGAACGCGGTACTGCTTGTGATCAAACAAATCGGCTATGAGGGTCTGGAAAGCGGTCACGGATTCAGGCATGAATTCAGCACGATTATGAACGAGCACg                                  >  2:317775/1‑151 (MQ=255)                                                                                                       gTCTCAGAGTTTGTTTTTGCCGGGCGCAACGATAAGAAGAGGCCAATCTGCGAGAACGCGGTACTGCTTGTGATCAAACAAATCGGCTATGAGGGTCTGGAAAGCGGTCACGGATTCAGGCATGAATTCAGCACGATTATGAACGAGCACg                                  >  2:347417/1‑151 (MQ=255)                                                                                                            agagTTTGTTTTTGCCGGGCGCAACGATAAGAAGAGGCCAATCTGCGAGAACGCGGTACTGCTTGTGATCAAACAAATCGGCTATGAGGGTCTGGAAAGCGGTCACGGATTCAGGCATGAATTCAGCACGATTATGAACGAGCACGAATgg                             <  1:216535/151‑1 (MQ=255)                                                                                                            agagTTTGTTTTTGCCGGGCGCAACGATAAGAAGAGGCCAATCTGCGAGAACGCGGTACTGCTTGTGATCAAACAAATCGGCTATGAGGGTCTGGAAAGCGGTCACGGATTCAGGCATGAATTCAGCACGATTATGAACGAGCACGAATgg                             <  2:391014/151‑1 (MQ=255)                                                                                                             gagTTTGTTTTTGCCGGGCGCAACGATAAGAAGAGGCCAATCTGCGAGAACGCGGTACTGCTTGTGATCAAACAAATCGGCTATGAGGGTCTGGAAAGCGGTCACGGATTCAGGCATGAATTCAGCACGATTATGAACGAGCACGAATGGc                            >  1:299425/1‑151 (MQ=255)                                                                                                                   gtttTTGCCGGGCGCAACGATAAGAAGAGGCCAATCTGCGAGAACGCGGTACTGCTTGTGATCAAACAAATCGGCTATGAGGGTCTGGAAAGCGGTCACGGATTCAGGCATGAATTCAGCACGATTATGAACGAGCACGAATGGCctgctg                      <  1:437658/151‑1 (MQ=255)                                                                                                                       ttGCCGGGCGCAACGATAAGAAGAGGCCAATCTGCGAGAACGCGGTACTGCTTGTGATCAAACAAATCGGCTATGAGGGTCTGGAAAGCGGTCACGGATTCAGGCATGAATTCAGCACGATTATGAACGAGCACGAATGGCCTGCTGACg                   <  2:464607/150‑1 (MQ=255)                                                                                                                         gCCGGGCGCAACGATAAGAAGAGGCCAATCTGCGAGAACGCGGTACTGCTTGTGATCAAACAAATCGGCTATGAGGGTCTGGAAAGCGGTCACGGATTCAGGCATGAATTCAGCACGATTATGAACGAGCACGAATGGCCTGCTGACGCt                 >  1:533421/1‑150 (MQ=255)                                                                                                                           cGGGCGCAACGATAAGAAGAGGCCAATCTGCGAGAACGCGGTACTGCTTGTGATCAAACAAATCGGCTATGAGGGTCTGGAAAGCGGTCACGGATTCAGGCATGAATTCAGCACGATTATGAACGAGCACGAATGGCCTGCTGACGCTAtt              <  2:512414/151‑1 (MQ=255)                                                                                                                           cGGGCGCAACGATAAGAAGAGGCCAATCTGCGAGAACGCGGTACTGCTTGTGATCAAACAAATCGGCTATGAGGGTCTGGAAAGCGGTCACGGATTCAGGCATGAATTCAGCACGATTATGAACGAGCACGAATGGCCTGCTGACGCTAtt              <  1:347424/151‑1 (MQ=255)                                                                                                                           cGGGCGCAACGATAAGAAGAGGCCAATCTGCGAGAACGCGGTACTGCTTGTGATCAAACAAATCGGCTATGAGGGTCTGGAAAGCGGTCACGGATTCAGGCATGAATTCAGCACGATTATGAACGAGCACGAATGGCCTGCTGACGCTAtt              <  1:381119/151‑1 (MQ=255)                                                                                                                               cgcAACGATAAGAAGAGGCCAATCTGCGAGAACGCGGTACTGCTTGTGATCAAACAAATCGGCTATGAGGGTCTGGAAAGCGGTCACGGATTCAGGCATGAATTCAGCACGATTATGAACGAGCACGAATGGCCTGCTGACGCTATTGa            >  1:398323/1‑149 (MQ=255)                                                                                                                               cgcAACGATAAGAAGAGGCCAATCTGCGAGAACGCGGTACTGCTTGTGATCAAACAAATCGGCTATGAGGGTCTGGAAAGCGGTCACGGATTCAGGCATGAATTCAGCACGATTATGAACGAGCACGAATGGCCTGCTGACGCTATTGa            >  1:615380/1‑149 (MQ=255)                                                                                                                                   aCGATAAGAAGAGGCCAATCTGTGAGAACGCGGTACTGCTTGTGATCAAACAAATCGGCTATGAGGGTCTGGAAAGCGGTCACGGATTCAGGCATGAATTCAGCACGATTATGAACGAGCACGAATGGCCTGCTGACGCTATTGAAGTGCa      <  1:210429/151‑1 (MQ=255)                                                                                                                                       tAAGAAGAGGCCAATCTGCGAGAACGCGGTACTGCTTGTGATCAAACAAATCGGCTATGAGGGTCTGGAAAGCGGTCACGGATTCAGGCATGAATTCAGCACGATTATGAACGAGCACGAATGGCCTGCTGACGCTATTGAAGTGCAACTg  >  1:112823/1‑151 (MQ=255)                                                                                                                                               |                                                                                                                                               ACCATCGACGCCAGTGTGATGAAAGGACGCAAAATTCATGTGGTTCCTATGTCAGACCAGGTAGTTGAACTTCTCACTACGCTAAGCTCCATCACCAAACCAGTCTCAGAGTTTGTTTTTGCCGGGCGCAACGATAAGAAGAAGCCAATCTGCGAGAACGCGGTACTGCTTGTGATCAAACAAATCGGCTATGAGGGTCTGGAAAGCGGTCACGGATTCAGGCATGAATTCAGCACGATTATGAACGAGCACGAATGGCCTGCTGACGCTATTGAAGTGCAACTG  >  NC_000913/2467304‑2467588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑