Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,517,257	A→G	S192S (AGT→AGC)	yfeA ←	putative diguanylate cyclase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,517,257	0	A	G	100.0%	142.3 / NA	38	S192S (AGT→AGC)	yfeA	putative diguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/25);  total (13/25)

CGGCAATAAAGTCGTTTTCATAAGGTGTGCACAACAGAAGTAGCAGCACGCTTAACGCTGCCAGCCAGCTTAAGGTAAATGCGCGTTTCTCTTTGCCCAACGACGGAGCGATATCCCTGCGCCACAATATCTGCGCAAAGTGGGGACTTACAATCATGCGAGTGAGATAGTAGAAAAGCATGTTGTAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCGAAAAAGGTGGATATCTTTAGCGGGAAATCAAAGAAGCTCCCC  >  NC_000913/2517112‑2517404                                                                                                                                                  |                                                                                                                                                    cGGCAATAAAGTCGTTTTCATAAGGTGTGCACAACAGAAGTAGCAGCACGCTTAACGCTGCCAGCCAGCTTAAGGTAAATGCGCGTTTCTCTTTGCCCAACGACGGAGCGATATCCCTGCGCCACAATATCTGCGCAAAGTGGGGGCTTAc                                                                                                                                                >  1:448002/1‑151 (MQ=255)  ggCAATAAAGTCGTTTTCATAAGGTGTGCACAACAGAAGTAGCAGCACGCTTAACGCTGCCAGCCAGCTTAAGGTAAATGCGCGTTTCTCTTTGCCCAACGACGGAGCGATATCCCTGCGCCACAATATCTGCGCAAAGTGGGGGCTTACa                                                                                                                                               <  1:235949/151‑1 (MQ=255)        aaaGTCGTTTTCATAAGGTGTGCACAACAGAAGTAGCAGCACGCTTAACGCTGCCAGCCAGCTTAAGGTAAATGCGCGTTTCTCTTTGCCCAACGACGGAGCGATATCCCTGCGCCACAATATCTGCGCAAAGTGGGGGCTTACAATCATg                                                                                                                                         <  1:391064/151‑1 (MQ=255)          aGTCGTTTTCATAAGGTGTGCACAACAGAAGTAGCAGCACGCTTAACGCTGCCAGCCAGCTTAAGGTAAATGCGCGTTTCTCTTTGCCCAACGACGGAGCGATATCCCTGCGCCACAATATCTGCGCAAAGTGGGGGCTTACAATCATGCg                                                                                                                                       >  1:248001/1‑151 (MQ=255)          aGTCGTTTTCATAAGGTGTGCACAACAGAAGTAGCAGCACGCTTAACGCTGCCAGCCAGCTTAAGGTAAATGCGCGTTTCTCTTTGCCCAACGACGGAGCGATATCCCTGCGCCACAATATCTGCGCAAAGTGGGGGCTTACAATCATGCg                                                                                                                                       <  1:419761/151‑1 (MQ=255)               ttttCATAAGGTGTGCACAACAGAAGTAGCAGCACGCTTAACGCTGCCAGCCAGCTTAAGGTAAATGCGCGTTTCTCTTTGCCCAACGACGGAGCGATATCCCTGCGCCACAATATCTGCGCAAAGTGGGGGCTTACAATCATGCGAGTg                                                                                                                                   >  1:349037/1‑150 (MQ=255)                      aaGGTGTGCACAACAGAAGTAGCAGCACGCTTAACGCTGCCAGCCAGCTTAAGGTAAATGCGCGTTTCTCTTTGCCCAACGACGGAGCGATATCCCTGCGCCACAATATCTGCGCAAAGTGGGGGCTTACAATCATGCGAGTGAGAtagt                                                                                                                            <  1:147837/150‑1 (MQ=255)                           gtgCACAACAGAAGTAGCAGCACGCTTAACGCTGCCAGCCAGCTTAAGGTAAATGCGCGTTTCTCTTTGCCCAACGACGGAGCGATATCCCTGCGCCACAATATCTGCGCAAAGTGGGGGCTTACAATCATGCGAGTGAGATAGTAGaaa                                                                                                                       <  2:104226/150‑1 (MQ=255)                           gtgCACAACAGAAGTAGCAGCACGCTTAACGCTGCCAGCCAGCTTAAGGTAAATGCGCGTTTCTCTTTGCCCAACGACGGAGCGATATCCCTGCGCCACAATATCTGCGCAAAGTGGGGGCTTACAATCATGCGAGTGAGATAGTAGaaa                                                                                                                       >  1:103688/1‑150 (MQ=255)                             gCACAACAGAAGTAGCAGCACGCTTAACGCTGCCAGCCAGCTTAAGGTAAATGCGCGTTTCTCTTTGCCCAACGACGGAGCGATATCCCTGCGCCACAATATCTGCGCAAAGTGGGGGCTTACAATCATGCGAGTGAGATAGTAGAAAAg                                                                                                                     <  1:436354/150‑1 (MQ=255)                               acaacaGAAGTAGCAGCACGCTTAACGCTGCCAGCCAGCTTAAGGTAAATGCGCGTTTCTCTTTGCCCAACGACGGAGCGATATCCCTGCGCCACAATATCTGCGCAAAGTGGGGGCTTACAATCATGCGAGTGAGATAGTAGAAAAGCAt                                                                                                                  <  1:234322/151‑1 (MQ=255)                                   caGAAGTAGCAGCACGCTTAACGCTGCCAGCCAGCTTAAGGTAAATGCGCGTTTCTCTTTGCCCAACGACGGAGCGATATCCCTGCGCCACAATATCTGCGCAAAGTGGGGGCTTACAATCATGCGAGTGAGATAGTAGAAAAGCAtgttg                                                                                                              <  2:140313/151‑1 (MQ=255)                                    aGAAGTAGCAGCACGCTTAACGCTGCCAGCCAGCTTAAGGTAAATGCGCGTTTCTCTTTGCCCAACGACGGAGCGATATCCCTGCGCCACAATATCTGCGCAAAGTGGGGGCTTACAATCATGCGAGTGAGATAGTAGAAAAGCAtgttgt                                                                                                             >  2:193215/1‑151 (MQ=255)                                    aGAAGTAGCAGCACGCTTAACGCTGCCAGCCAGCTTAAGGTAAATGCGCGTTTCTCTTTGCCCAACGACGGAGCGATATCCCTGCGCCACAATATCTGCGCAAAGTGGGGGCTTACAATCATGCGAGTGAGATAGTAGAAAAGCAtgttgt                                                                                                             <  1:573853/151‑1 (MQ=255)                                     gAAGTAGCAGCACGCTTAACGCTGCCAGCCAGCTTAAGGTAAATGCGCGTTTCTCTTTGCCCAACGACGGAGCGATATCCCTGCGCCACAATATCTGCGCAAAGTGGGGGCTTACAATCATGCGAGTGAGATAGTAGAAAAGCATGTTGTa                                                                                                            <  2:15562/151‑1 (MQ=255)                                       aGTAGCAGCACGCTTAACGCTGCCAGCCAGCTTAAGGTAAATGCGCGTTTCTCTTTGCCCAACGACGGAGCGATATCCCTGCGCCACAATATCTGCGCAAAGTGGGGGCTTACAATCATGCGAGTGAGATAGTAGAAAAGCATGTTGTaa                                                                                                           >  1:593085/1‑150 (MQ=255)                                           gcagcaCGCTTAACGCTGCCAGCCAGCTTAAGGTAAATGCGCGTTTCTCTTTGCCCAACGACGGAGCGATATCCCTGCGCCACAATATCTGCGCAAAGTGGGGGCTTACAATCATGCGAGTGAGATAGTAGAAAAGCATGTTGTAAATCAg                                                                                                      <  2:183785/151‑1 (MQ=255)                                             agcaCGCTTAACGCTGCCAGCCAGCTTAAGGTAAATGCGCGTTTCTCTTTGCCCAACGACGGAGCGATATCCCTGCGCCACAATATCTGCGCAAAGTGGGGGCTTACAATCATGCGAGTGAGATAGTAGAAAAGCATGTTGTAAATCAGca                                                                                                    <  1:1173/151‑1 (MQ=255)                                                  gCTTAACGCTGCCAGCTAGCTTAAGGTAAATGCGCGTTTCTCTTTGCCCAACGACGGAGCGATATCCCTGCGCCACAATATCTGCGCAAAGTGGGGGCTTACAATCATGCGAGTGAGATAGTAGAAAAGCATGTTGTAAATCAGCACAGCg                                                                                               <  2:38855/151‑1 (MQ=255)                                                      aaCGCTGCCAGCCAGCTTAAGGTAAATGCGCGTTTCTCTTTGCCCAACGACGGAGCGATATCCCTGCGCCACAATATCTGCGCAAAGTGGGGGCTTACAATCATGCGAGTGAGATAGTAGAAAAGCATGTTGTAAATCAGCACAGCGGTg                                                                                            <  1:352448/150‑1 (MQ=255)                                                      aaCGCTGCCAGCCAGCTTAAGGTAAATGCGCGTTTCTCTTTGCCCAACGACGGAGCGATATCCCTGCGCCACAATATCTGCGCAAAGTGGGGGCTTACAATCATGCGAGTGAGATAGTAGAAAAGCATGTTGTAAATCAGCACAGCGGTGa                                                                                           <  2:587212/151‑1 (MQ=255)                                                       aCGCTGCCAGCCAGCTTAAGGTAAATGCGCGTTTCTCTTTGCCCAACGACGGAGCGATATCCCTGCGCCACAATATCTGCGCAAAGTGGGGGCTTACAATCATGCGAGTGAGATAGTAGAAAAGCATGTTGTAAATCAGCACAGCGGTGaa                                                                                          <  1:125290/151‑1 (MQ=255)                                                        cGCTGCCAGCCAGCTTAAGGTAAATGCGCGTTTCTCTTTGCCCAACGACGGAGCGATATCCCTGCGCCACAATATCTGCGCAAAGTGGGGGCTTACAATCATGCGAGTGAGATAGTAGAAAAGCATGTTGTAAATCAGCACAGCGGTGaa                                                                                          >  2:51161/1‑150 (MQ=255)                                                                                tGCGCGTTTCTCTTTGCCCAACGACGGAGCGATATCCCTGCGCCACAATATCTGCGCAAAGTGGGGGCTTACAATCATGCGAGTGAGATAGTAGAAAAGCATGTTGTAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTg                                                                 >  1:407054/1‑151 (MQ=255)                                                                                   gcgTTTCTCTTTGCCCAACGACGGAGCGATATCCCTGCGCCACAATATCTGCGCAAAGTGGGGGCTTACAATCATGCGAGTGAGATAGTAGAAAAGCATGTTGTAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGaaa                                                              <  1:455117/151‑1 (MQ=255)                                                                                    cgTTTCTCTTTGCCCAACGACGGAGCGATATCCCTGCGCCACAATATCTGCGCAAAGTGGGGGCTTACAATCATGCGAGTGAGATAGTAGAAAAGCATGTTGTAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGaaa                                                              >  1:42007/1‑150 (MQ=255)                                                                                       ttCTCTTTGCCCAACGACGGAGCGATATCCCTGCGCCACAATATCTGCGCAAAGTGGGGGCTTACAATCATGCGAGTGAGATAGTAGAAAAGCATGTTGTAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATgg                                                          >  2:550415/1‑151 (MQ=255)                                                                                        tctcTTTGCCCAACGACGGAGCGATATCCCTGCGCCACAATATCTGCGCAAAGTGGGGGCTTACAATCATGCGAGTGAGATAGTAGAAAAGCATGTTGTAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGGCCGTGAAAATgg                                                          <  1:552543/150‑1 (MQ=255)                                                                                                  cAACGACGGAGCGATATCCCTGCGCCACAATATCTGCGCAAAGTGGGGGCTTACAATCATGCGAGTGAGATAGTAGAAAAGCATGTTGTAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCAtag                                               <  2:67466/151‑3 (MQ=255)                                                                                                      gacgGAGCGATATCCCTGCGCCACAATATCTGCGCAAAGTGGGGGCTTACAATCATGCGAGTGAGATAGTAGAAAAGCATGTTGTAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCg                                            >  1:216929/1‑150 (MQ=255)                                                                                                             cGATATCCCTGCGCCACAATATCTGCGCAAAGTGGGGGCTTACAATCATGCGAGTGAGATAGTAGAAAAGCATGTTGTAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCGAAAAAGGt                                    >  2:570088/1‑151 (MQ=255)                                                                                                                    ccTGCGCCACAATATCTGCGCAAAGTGGGGGCTTACAATCATGCGAGTGAGATAGTAGAAAAGCATGTTGTAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCGAAAAAGGTGGATATc                             <  2:386452/151‑1 (MQ=255)                                                                                                                       gcgcCACAATATCTGCGCAAAGTGGGGGCTTACAATCATGCGAGTGAGATAGTAGAAAAGCATGTTGTAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCGAAAAAGGTGGATATCttt                          <  2:381468/151‑1 (MQ=255)                                                                                                                         gcCACAATATCTGCGCAAAGTGGGGGCTTACAATCATGCGAGTGAGATAGTAGAAAAGCATGTTGTAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCGAAAAAGGTGGATATCTTTAg                        <  2:512683/151‑1 (MQ=255)                                                                                                                          ccACAATATCTGCGCAAAGTGGGGGCTTACAATCATGCGAGTGAGATAGTAGAAAAGCATGTTGTAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCGAAAAAGGTGGATATCTTTAGc                       >  1:327216/1‑151 (MQ=255)                                                                                                                                     gcgcAAAGTGGGGGCTTACAATCATGCGAGTGAGATAGTAGAAAAGCATGTTGTAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCGAAAAAGGTGGATATCTTTAGCGGGAAATCa              <  2:152613/149‑1 (MQ=255)                                                                                                                                        cAAAGTGGGGGCTTACAATCATGCGAGTGAGATAGTAGAAAAGCATGTTGTAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCGAAAAAGGTGGATATCTTTAGCGGGAAATCAaagaa         <  2:424317/151‑1 (MQ=255)                                                                                                                                           aGTGGGGGCTTACAATCATGCGAGTGAGATAGTAGAAAAGCATGTTGTAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCGAAAAAGGTGGATATCTTTAGCGGGAAATCAAAGAAGCt      <  1:335042/151‑1 (MQ=255)                                                                                                                                               ggggCTTACAATCATGCGAGTGAGATAGTAGAAAAGCATGTTGTAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCGAAAAAGGTGGATATCTTTAGCGGGAAATCAAAGAAGCTcccc  <  2:37371/151‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                    CGGCAATAAAGTCGTTTTCATAAGGTGTGCACAACAGAAGTAGCAGCACGCTTAACGCTGCCAGCCAGCTTAAGGTAAATGCGCGTTTCTCTTTGCCCAACGACGGAGCGATATCCCTGCGCCACAATATCTGCGCAAAGTGGGGACTTACAATCATGCGAGTGAGATAGTAGAAAAGCATGTTGTAAATCAGCACAGCGGTGAAAAGGCTTAGCAAATCAACGACCGTGAAAATGGCATCCGCATCGCCGAAAAAGGTGGATATCTTTAGCGGGAAATCAAAGAAGCTCCCC  >  NC_000913/2517112‑2517404

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑