Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,611,725	A→G	H90R (CAC→CGC)	hyfJ →	putative processing element hydrogenase 4

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,611,725	0	A	G	100.0%	135.1 / NA	39	H90R (CAC→CGC)	hyfJ	putative processing element hydrogenase 4
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/15);  total (24/15)

CGTTGGCGTGATTGATTGTCTGAATGTCGCCTTCCGCTGCCCACTGACGGAATACGAAGATTGGCTTGCACTGGTCGAAGAGGAGCAAGCCCGACGTAAGATGCTGGGGGTGATGACTTTTGGTGAGATTGTTATTGACGCCAGCCACACCGCCCTGTTGACCCGGGCATTCGCGCCACTGGCGGATGACGCGACGTCTGTGTGGCAGGCGCGTAGCATTCAATTCATTCATCTGTTGGATGAAATTGTGCAGGAACCGGCCATCTATCTGATGGCCAGAAAAATT  >  NC_000913/2611579‑2611864                                                                                                                                                   |                                                                                                                                            cGTTGGCGTGATTGATTGTCTGAATGTCGCCTTCCGCTGCCCACTGACGGAATACGAAGATTGGCTTGCACTGGTCGAAGAGGAGCAAGCCCGACGTAAGATGCTGGGGGTGATGACTTTTGGTGAGATTGTTATTGACGCCAGCCGCAcc                                                                                                                                         >  2:127288/1‑151 (MQ=255)   ttGGCGTGATTGATTGTCTGAATGTCGCCTTCCGCTGCCCACTGACGGAATACGAAGATTGGCTTGCACTGGTCGAAGAGGAGCAAGCCCGACGTAAGATGCTGGGGGTGATGACTTTTGGTGAGATTGTTATTGACGCCAGCCGCACCGc                                                                                                                                       >  2:216271/1‑151 (MQ=255)        gTGATTGATTGTCTGAATGTCGCCTTCCGCTGCCCACTGACGGAATACGAAGATTGGCTTGCACTGGTCGAAGAGGAGCAAGCCCGACGTAAGATGCTGGGGGTGATGACTTTTGGTGAGATTGTTATTGACGCCAGCCGCACCGCCCTGt                                                                                                                                  >  1:619340/1‑151 (MQ=255)             tgattgTCTGAATGTCGCCTTCCGCTGCCCACTGACGGAATACGAAGATTGGCTTGCACTGGTCGAAGAGGAGCAAGCCCGACGTAAGATGCTGGGGGTGATGACTTTTGGTGAGATTGTTATTGACGCCAGCCGCACCGCCCTGTTGAcc                                                                                                                             >  2:261437/1‑151 (MQ=255)                      gAATGTCGCCTTCCGCTGCCCACTGACGGAATACGAAGATTGGCTTGCACTGGTCGAAGAGGAGCAAGCCCGACGTAAGATGCTGGGGGTGATGACTTTTGGTGAGATTGTTATTGACGCCAGCCGCACCGCCCTGTTGACCCGGGCATTc                                                                                                                    >  2:430487/1‑151 (MQ=255)                       aaTGTCGCCTTCCGCTGCCCACTGACGGAATACGAAGATTGGCTTGCACTGGTCGAAGAGGAGCAAGCCCGACGTAAGATGCTGGGGGTGATGACTTTTGGTGAGATTGTTATTGACGCCAGCCGCACCGCCCTGTTGACCCGGGCATTcg                                                                                                                   <  1:70069/151‑1 (MQ=255)                              ccTTCCGCTGCCCACTGACGGAATACGAAGATTGGCTTGCACTGGTCGAAGAGGAGCAAGCCCGACGTAAGATGCTGGGGGTGATGACTTTTGGTGAGATTGTTATTGACGCCAGCCGCACCGCCCTGTTGACCCGGGCATTCGCGCCACt                                                                                                            <  1:2953/151‑1 (MQ=255)                                  ccGCTGCCCACTGACGGAATACGAAGATTGGCTTGCACTGGTCGAAGAGGAGCAAGCCCGACGTAAGATGCTGGGGGTGATGACTTTTGGTGAGATTGTTATTGACGCCAGCCGCACCGCCCTGTTGACCCGGGCATTCGCGCCACTGGCg                                                                                                        >  1:407084/1‑151 (MQ=255)                                   cGCTGCCCACTGACGGAATACGAAGATTGGCTTGCACTGGTCGAAGAGGAGCAAGCCCGACGTAAGATGCTGGGGGTGATGACTTTTGGTGAGATTGTTATTGACGCCAGCCGCACCGCCCTGTTGACCCGGGCATTCGCGCCACTGGCgg                                                                                                       <  2:74477/151‑1 (MQ=255)                                   cGCTGCCCACTGACGGAATACGAAGATTGGCTTGCACTGGTCGAAGAGGAGCAAGCCCGACGTAAGATGCTGGGGGTGATGACTTTTGGTGAGATTGTTATTGACGCCAGCCGCACCGCCCTGTTGACCCGGGCATTCGCGCCACTGGCgg                                                                                                       >  1:75939/1‑151 (MQ=255)                                     cTGCCCACTGACGGAATACGAAGATTGGCTTGCACTGGTCGAAGAGGAGCAAGCCCGACGTAAGATGCTGGGGGTGATGACTTTTGGTGAGATTGTTATTGACGCCAGCCGCACCGCCCTGTTGACCCGGGCATTCGCGCCACTGGCGGa                                                                                                      >  2:473143/1‑150 (MQ=255)                                      tGCCCACTGACGGAATACGAAGATTGGCTTGCACTGGTCGAAGAGGAGCAAGCCCGACGTAAGATGCTGGGGGTGATGACTTTTGGTGAGATTGTTATTGACGCCAGCCGCACCGCCCTGTTGACCCGGGCATTCGCGCCACTGGCGGATg                                                                                                    >  1:601665/1‑151 (MQ=255)                                                              tGGCTTGCACTGGTCGAAGAGGAGCAAGCCCGACGTAAGGTGCTGGGGGTGATGACTTTTGGTGAGATTGTTATTGACGCCAGCCGCACCGCCCTGTTGACCCGGGCATTCGCGCCACTGGCGGATGACGCGACGTCTGTGTGGCAGgcgc                                                                            >  1:198675/1‑151 (MQ=255)                                                               ggCTTGCACTGGTCGAAGAGGAGCAAGCCCGACGTAAGATGCTGGGGGTGATGACTTTTGGTGAGATTGTTATTGACGCCAGCCGCACCGCCCTGTTGACCCGGGCATTCGCGCCACTGGCGGATGACGCGACGTCTGTGTGGCAGGcgcg                                                                           <  2:374162/151‑1 (MQ=255)                                                                  ttGCACTGGTCGAAGAGGAGCAAGCCCGACGTAAGATGCTGGGGGTGATGACTTTTGGTGAGATTGTTATTGACGCCAGCCGCACCGCCCTGTTGACCCGGGCATTCGCGCCACTGGCGGATGACGCGACGTCTGTGTGGCAGGcgcg                                                                           >  1:1059/1‑148 (MQ=255)                                                                   tGCACTGGTCGAAGAGGAGCAAGCCCGACGTAAGATGCTGGGGGTGATGACTTTTGGTGAGATTGTTATTGACGCCAGCCGCACCGCCCTGTTGACCCGGGCATTCGCGCCACTGGCGGATGACGCGACGTCTGTGTGGCAGGCGCGTa                                                                         <  1:470860/149‑1 (MQ=255)                                                                   tGCACTGGTCGAAGAGGAGCAAGCCCGACGTAAGATGCTGGGGGTGATGACTTTTGGTGAGATTGTTATTGACGCCAGCCGCACCGCCCTGTTGACCCGGGCATTCGCGCCACTGGCGGATGACGCGACGTCTGTGTGGCAGGCGCGTAGc                                                                       >  1:111702/1‑151 (MQ=255)                                                                     cACTGGTCGAAGAGGAGCAAGCCCGACGTAAGATGCTGGGGGTGATGACTTTTGGTGAGATTGTTATTGACGCCAGCCGCACCGCCCTGTTGACCCGGGCATTCGCGCCACTGGCGGATGACGCGACGTCTGTGTGGCAGGCGCGTAGCat                                                                     <  1:421179/151‑1 (MQ=255)                                                                     cACTGGTCGAAGAGGAGCAAGCCCGACGTAAGATGCTGGGGGTGATGACTTTTGGTGAGATTGTTATTGACGCCAGCCGCACCGCCCTGTTGACCCGGGCATTCGCGCCACTGGCGGATGACGCGACGTCTGTGTGGCAGGCGCGTAGCat                                                                     >  2:152288/1‑151 (MQ=255)                                                                         ggTCGAAGAGGAGCAAGCCCGACGTAAGATGCTGGGGGTGATGACTTTTGGTGAGATTGTTATTGACGCCAGCCGCACCGCCCTGTTGACCCGGGCATTCGCGCCACTGGCGGATGACGCGACGTCTGTGTGGCAGGCGCGTAGCattcaa                                                                 <  1:468989/151‑1 (MQ=255)                                                                          gTCGAAGAGGAGCAAGCCCGACGTAAGATGCTGGGGGTGATGACTTTTGGTGAGATTGTTATTGACGCCAGCCGCACCGCCCTGTTGACCCGGGCATTCGCGCCACTGGCGGATGACGCGACGTCTGTGTGGCAGGCGCGTAGCattcaat                                                                <  1:11268/151‑1 (MQ=255)                                                                            cGAAGAGGAGCAAGCCCGACGTAAGATGCTGGGGGTGATGACTTTTGGTGAGATTGTTATTGACGCCAGCCGCACCGCCCTGTTGACCCGGGCATTCGCGCCACTGGCGGATGACGCGACGTCTGTGTGGCAGGCGCGTAGCattcaatt                                                               <  1:132871/150‑1 (MQ=255)                                                                             gAAGAGGAGCAAGCCCGACGTAAGATGCTGGGGGTGATGACTTTTGGTGAGATTGTTATTGACGCCAGCCGCACCGCCCTGTTGACCCGGGCATTCGCGCCACTGGCGGATGACGCGACGTCTGTGTGGCAGGCGCGTAGCattcaattc                                                              >  2:397080/1‑150 (MQ=255)                                                                                gaggagCAAGCCCGACGTAAGATGCTGGGGGTGATGACTTTTGGTGAGATTGTTATTGACGCCAGCCGCACCGCCCTGTTGACCCGGGCATTCGCGCCACTGGCGGATGACGCGACGTCTGTGTGGCAGGCGCGTAGCATTCAattcattc                                                          >  1:611210/1‑151 (MQ=255)                                                                                    agCAAGCCCGACGTAAGATGCTGGGGGTGATGACTTTTGGTGAGATTGTTATTGACGCCAGCCGCACCGCCCTGTTGACCCGGGCATTCGCGCCACTGGCGGATGACGCGACGTCTGTGTGGCAGGCGCGTAGCATTCAATTCATTCATc                                                       >  2:48913/1‑150 (MQ=255)                                                                                    agCAAGCCCGACGTAAGATGCTGGGGGTGATGACTTTTGGTGAGATTGTTATTGACGCCAGCCGCACCGCCCTGTTGACCCGGGCATTCGCGCCACTGGCGGATGACGCGACGTCTGTGTGGCAGGCGCGTAGCATTCAATTCATTCATCt                                                      <  1:454439/151‑1 (MQ=255)                                                                                       aaGCCCGACGTAAGATGCTGGGGGTGATGACTTTTGGTGAGATTGTTATTGACGCCAGCCGCACCGCCCTGTTGACCCGGGCATTCGCGCCACTGGCGGATGACGCGACGTCTGTGTGGCAGGCGCGTAGCATTCAATTCATTCATCTGtt                                                   >  2:594447/1‑151 (MQ=255)                                                                                              aCGTAAGATGCTGGGGGTGATGACTTTTGGTGAGATTGTTATTGACGCCAGCCGCACCGCCCTGTTGACCCGGGCATTCGCGCCACTGGCGGATGACGCGACGTCTGTGTGGCAGGCGCGTAGCATTCAATTCATTCATCTGTTGGATGaa                                            <  2:407077/151‑1 (MQ=255)                                                                                               cGTAAGATGCTGGGGGTGATGACTTTTGGTGAGATTGTTATTGACGCCAGCCGCACCGCCCTGTTGACCCGGGCATTCGCGCCACTGGCGGATGACGCGACGTCTGTGTGGCAGGCGCGTAGCATTCAATTCATTCATCTGTTGGATGaa                                            >  1:579627/1‑150 (MQ=255)                                                                                               cGTAAGATGCTGGGGGTGATGACTTTTGGTGAGATTGTTATTGACGCCAGCCGCACCGCCCTGTTGACCCGGGCATTCGCGCCACTGGCGGATGACGCGACGTCTGTGTGGCAGGCGCGTAGCATTCAATTCATTCATCTGTTGGATGaa                                            >  1:137574/1‑150 (MQ=255)                                                                                                  aaGATGCTGGGGGTGATGACTTTTGGTGAGATTGTTATTGACGCCAGCCGCACCGCCCTGTTGACCCGGGCATTCGCGCCACTGGCGGATGACGCGACGTCTGTGTGGCAGGCGCGTAGCATTCAATTCATTCATCTGTTGGATGAAATtg                                        <  1:5011/151‑1 (MQ=255)                                                                                                             ggTGATGACTTTTGGTGAGATTGTTATTGACGCCAGCCGCACCGCCCTGTTGACCCGGGCATTCGCGCCACTGGCGGATGACGCGACGTCTGTGTGGCAGGCGCGTAGCATTCAATTCATTCATCTGTTGGATGAAATTGTGCAGGAACCg                             <  1:143072/151‑1 (MQ=255)                                                                                                                gatgaCTTTTGGTGAGATTGTTATTGACGCCAGCCGCACCGCCCTGTTGACCCGGGCATTCGCGCCACTGGCGGATGACGCGACGTCTGTGTGGCAGGCGCGTAGCATTCAATTCATTCATCTGTTGGATGAAATTGTGCAGGAACCGGcc                          >  2:440213/1‑151 (MQ=255)                                                                                                                         tGGTGAGATTGTTATTGACGCCAGCCGCACCGCCCTGTTGACCCGGGCATTCGCGCCACTGGCGGATGACGCGACGTCTGTGTGGCAGGCGCGTAGCATTCAATTCATTCATCTGTTGGATGAAATTGTGCAGGAACCGGCCatctatct                  <  1:235386/150‑1 (MQ=255)                                                                                                                         tGGTGAGATTGTTATTGACGCCAGCCGCACCGCCCTGTTGACCCGGGCATTCGCGCCACTGGCGGATGACGCGACGTCTGTGTGGCAGGCGCGTAGCATTCAATTCATTCATCTGTTGGATGAAATTGTGCAGGAACCGGCCatctatct                  >  2:603300/1‑150 (MQ=255)                                                                                                                               gaTTGTTATTGACGCCAGCCGCACCGCCCTGTTGACCCGGGCATTCGCGCCACTGGCGGATGACGCGACGTCTGTGTGGCAGGCGCGTAGCATTCAATTCATTCATCTGTTGGATGAAATTGTGCAGGAACCGGCCATCTATCTGATGGcc           <  2:301511/151‑1 (MQ=255)                                                                                                                                aTTGTTATTGACGCCAGCCGCACCGCCCTGTTGACCCGGGCATTCGCGCCACTGGCGGATGACGCGACGTCTGTGTGGCAGGCGCGTAGCATTCAATTCATTCATCTGTTGGATGAAATTGTGCAGGAACCGGCCATCTATCTGATGGcc           >  2:369077/1‑150 (MQ=255)                                                                                                                                   gTTATTGACGCCAGCCGCACCGCCCTGTTGACCCGGGCATTCGCGCCACTGGCGGATGACGCGACGTCTGTGTGGCAGGCGCGTAGCATTCAATTCATTCATCTGTTGGATGAAATTGTGCAGGAACCGGCCATCTATCTGATGGCCAGa        >  1:408347/1‑150 (MQ=255)                                                                                                                                        tGACGCCAGCCGCACCGCCCTGTTGACCCGGGCATTCGCGCCACTGGCGGATGACGCGACGTCTGTGTGGCAGGCGCGTAGCATTCAATTCATTCATCTGTTGGATGAAATTGTGCAGGAACCGGCCATCTATCTGATGGCCAGAAAAAtt  >  1:236906/1‑151 (MQ=255)                                                                                                                                                   |                                                                                                                                            CGTTGGCGTGATTGATTGTCTGAATGTCGCCTTCCGCTGCCCACTGACGGAATACGAAGATTGGCTTGCACTGGTCGAAGAGGAGCAAGCCCGACGTAAGATGCTGGGGGTGATGACTTTTGGTGAGATTGTTATTGACGCCAGCCACACCGCCCTGTTGACCCGGGCATTCGCGCCACTGGCGGATGACGCGACGTCTGTGTGGCAGGCGCGTAGCATTCAATTCATTCATCTGTTGGATGAAATTGTGCAGGAACCGGCCATCTATCTGATGGCCAGAAAAATT  >  NC_000913/2611579‑2611864

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑