Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,647,150 G→A A59V (GCT→GTT)  pbpC ← penicillin‑insensitive murein repair transglycosylase; inactive transpeptidase domain protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,647,1500GA96.3% 97.6 / ‑3.8 27A59V (GCT→GTT) pbpCpenicillin‑insensitive murein repair transglycosylase; inactive transpeptidase domain protein
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (14/12);  total (14/13)
Fisher's exact test for biased strand distribution p-value = 4.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.03e-01

GAGATCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAGCATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCC  >  NC_000913/2647005‑2647294
                                                                                                                                                 |                                                                                                                                                
gagaTCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATc                                                                                                                                              <  1:136662/150‑1 (MQ=255)
               tgcGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAgc                                                                                                                              >  1:414095/1‑151 (MQ=255)
                              gaATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTgc                                                                                                               >  2:128131/1‑151 (MQ=255)
                              gaATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTgc                                                                                                               <  1:45787/151‑1 (MQ=255)
                                              ggATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTg                                                                                               >  1:153762/1‑151 (MQ=255)
                                                 tGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCgg                                                                                            >  1:325365/1‑151 (MQ=255)
                                                     tCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAGCATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGAt                                                                                         <  2:152265/150‑1 (MQ=255)
                                                        aGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGAc                                                                                     >  2:251668/1‑151 (MQ=255)
                                                              aGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATg                                                                               >  2:422050/1‑151 (MQ=255)
                                                                      tCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCa                                                                             <  2:112171/145‑1 (MQ=255)
                                                                      tCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCa                                                                             <  1:112525/145‑1 (MQ=255)
                                                                       cATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTa                                                                       <  2:527636/150‑1 (MQ=255)
                                                                        aTAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGt                                                                        >  1:49451/1‑148 (MQ=255)
                                                                         tAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTag                                                                      >  1:630770/1‑149 (MQ=255)
                                                                                 cAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGt                                                            >  2:489972/1‑151 (MQ=255)
                                                                                  aGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGtt                                                           <  2:439169/151‑1 (MQ=255)
                                                                                        tCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCt                                                      <  1:378196/150‑1 (MQ=255)
                                                                                              tAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTcccc                                               >  2:73306/1‑151 (MQ=255)
                                                                                                      agaAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCa                                       <  1:349424/151‑1 (MQ=255)
                                                                                                                tCGATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGaaaaa                                <  1:257227/148‑1 (MQ=255)
                                                                                                                  gATTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAgg                              >  1:586718/1‑148 (MQ=255)
                                                                                                                   aTTGTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGgaga                           >  2:115547/1‑150 (MQ=255)
                                                                                                                      gTTACCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGGCCACTCGTGCGGGGTTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAg                        <  1:11059/150‑1 (MQ=255)
                                                                                                                         aCCGGATAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGgc                     <  1:409509/150‑1 (MQ=255)
                                                                                                                              aTAACGCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCt               <  2:422336/151‑1 (MQ=255)
                                                                                                                                   gCCAGATGCCGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCa           >  1:289839/1‑150 (MQ=255)
                                                                                                                                            cGTCAACATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATcc  >  2:430971/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GAGATCTTGCCATGCTGCGCGCGCCACCGAGAATGGATTCACCCCCGGATGCTTCCAGAACCAGCGATCTTCATAATTGATCAGCGCTTCAAGGTAACGTGGAGAAACATCTTCGATTGTTACCGGATAACGCCAGATGCCGTCAGCATCGGCGAAGCGCCAGAGCGGCGTACCATCCTGCGCCACGACCACTCGTGCGGGATTGACTTCATGCAGCGGTAGAGGCCAGAGTTTATCTGCTCCCCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCC  >  NC_000913/2647005‑2647294

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑