Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	261,402	G→A	A339T (GCC→ACC)	proB →	gamma‑glutamate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	261,402	0	G	A	100.0%	135.5 / NA	36	A339T (GCC→ACC)	proB	gamma‑glutamate kinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/18);  total (18/18)

CAGCTCCCTGTTGCCGAAAGGCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAAGTCATCCGCATTTGCAACCTCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTGCCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCACCGTGATGACATGATTACCCGTTAAGGAGCAGGCTGATGCTGGAACAAATGGGCATTGCCGCGAAGCAAGCCTCGTATAA  >  NC_000913/261263‑261546                                                                                                                                            |                                                                                                                                                 cAGCTCCCTGTTGCCGAAAGGCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAAGTCATCCGCATTTGCAACCTCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGAcaccac                                                                                                                                       >  2:64093/1‑151 (MQ=255)     tCCCTGTTGCCGAAAGGCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAAGTCATCCGCATTTGCAACCTCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGc                                                                                                                                   >  1:513776/1‑151 (MQ=255)        cTGTTGCCGAAAGGCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAAGTCATCCGCATTTGCAACCTCGAAGGCCGCGATATCGCCCACGGCGGCAGTCGTTACAACACCGATGCATTACGCCGTATTACCGGACACCCCTCg                                                                                                                                    >  2:78816/1‑147 (MQ=255)          gTTGCCGAAAGGCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAAGTCATCCGCATTTGCAACCTCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGa                                                                                                                               <  2:54513/150‑1 (MQ=255)            tGCCGAAAGGCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAAGTCATCCGCATTTGCAACCTCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAAt                                                                                                                            >  1:559213/1‑151 (MQ=255)             gCCGAAAGGCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAAGTCATCCGCATTTGCAACCTCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAAt                                                                                                                            <  2:471914/150‑1 (MQ=255)              ccGAAAGGCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAAGTCATCCGCATTTGCAACCTCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTg                                                                                                                          <  1:99852/151‑1 (MQ=255)              ccGAAAGGCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAAGTCATCCGCATTTACAACCTCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTg                                                                                                                          >  1:26752/1‑151 (MQ=255)               cGAAAGGCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAAGTCATCCGCATTTGCAACCTCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTg                                                                                                                          >  1:60476/1‑150 (MQ=255)                  aaGGCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAAGTCATCCGCATTTGCAACCTCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGAt                                                                                                                        <  1:418547/149‑1 (MQ=255)                      cATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAAGTCATCCGCATTTGCAACCTCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATa                                                                                                                  <  2:60476/151‑1 (MQ=255)                          aaaaGCGTGACTGGCAATTTCTCGCGTGGTGAAGTCATCCGCATTTGCAACCTCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTg                                                                                                               <  2:303262/150‑1 (MQ=255)                               cGTGACTGGCAATTTCTCGCGTGGTGAAGTCATCCGCATTTGCAACCTCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGatat                                                                                                         >  1:137148/1‑151 (MQ=255)                               cGTGACTGGCAATTTCTCGCGTGGTGAAGTCATCCGCATTTGCAACCTCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGatat                                                                                                         >  2:70183/1‑151 (MQ=255)                                   aCTGGCAATTTCTCGCGTGGTGAAGTCATCCGCATTTGCAACCTCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAAt                                                                                                     <  1:193796/151‑1 (MQ=255)                                        cAATTTCTCGCGTGGTGAAGTCATCCGCATTTGCAACCTCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGc                                                                                                >  2:416960/1‑151 (MQ=255)                                                     ggtgAAGTCATCCGCATTTGCAACCTCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGt                                                                                     >  2:339111/1‑149 (MQ=255)                                                            tCATCCGCATTTGCAACCTCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCACCGtg                                                                             >  2:104789/1‑150 (MQ=255)                                                              aTCCGCATTTGCAACCTCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCACCGtgatg                                                                          <  2:585612/151‑1 (MQ=255)                                                                         cAACCTCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCACCGTGATGACATGATTa                                                                 <  2:26752/149‑1 (MQ=255)                                                                           aCCTCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCACCGTGATGACATGATTACCCg                                                             >  1:366417/1‑151 (MQ=255)                                                                             cTCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCACCGTGATGACATGATTACCCGtt                                                           >  1:484901/1‑151 (MQ=255)                                                                               cGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCACCGTGATGACATGATTACCCGtt                                                           <  1:58509/149‑1 (MQ=255)                                                                                     ccGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCACCGTGATGACATGATTACCCGTTAAGGAGCa                                                   <  2:112471/151‑1 (MQ=255)                                                                                      cgcgATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCACCGTGATGACATGATTACCCGTTAAGGAGCAg                                                  <  1:518873/151‑1 (MQ=255)                                                                                               gCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCACCGTGATGACATGATTACCCGTTAAGGAGCAGGCTGATGCt                                         <  2:337807/151‑1 (MQ=255)                                                                                                  cACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCACCGTGATGACATGATTACCCGTTAAGGAGCAGGCTGATGCTgg                                       >  2:119352/1‑150 (MQ=255)                                                                                                          cAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCACCGTGATGACATGATTACCCGTTAAGGAGCAGGCTGATGCTGGAACAAATgg                              >  2:489891/1‑151 (MQ=255)                                                                                                          cAGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCACCGTGATGACATGATTACCCGTTAAGGAGCAGGCTGATGCTGGAACAAATgg                              <  2:502684/151‑1 (MQ=255)                                                                                                           aGTCGTTACAACAGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCACCGTGATGACATGATTACCCGTTAAGGAGCAGGCTGATGCTGGAACAAATggg                             >  2:169599/1‑151 (MQ=255)                                                                                                                       aGCGATGCATTACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCACCGTGATGACATGATTACCCGTTAAGGAGCAGGCTGATGCTGGAACAAATGGGCATTGCCGCGaa                 <  1:330013/151‑1 (MQ=255)                                                                                                                                 tACGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCACCGTGATGACATGATTACCCGTTAAGGAGCAGGCTGATGCTGGAACAAATGGGCATTGCCGCGAAGCAAGCCTc        <  2:180868/150‑1 (MQ=255)                                                                                                                                   cGCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCACCGTGATGACATGATTACCCGTTAAGGAGCAGGCTGATGCTGGAACAAATGGGCATTGCCGCGAAGCAAGCCTCGt      >  1:556019/1‑150 (MQ=255)                                                                                                                                    gCCGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCACCGTGATGACATGATTACCCGTTAAGGAGCAGGCTGATGCTGGAACAAATGGGCATTGCCGCGAAGCAAGCCTCGtat    <  2:514828/151‑1 (MQ=255)                                                                                                                                      cGTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCACCGTGATGACATGATTACCCGTTAAGGAGCAGGCTGATGCTGGAACAAATGGGCATTGCCGCGAAGCAAGCCTCGTATaa  <  1:198393/151‑1 (MQ=255)                                                                                                                                       gTATTACCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCACCGTGATGACATGATTACCCGTTAAGGAGCAGGCTGATGCTGGAACAAATGGGCATTGCCGCGAAGCAAGCCTCGTATaa  >  2:297104/1‑150 (MQ=255)                                                                                                                                            |                                                                                                                                                 CAGCTCCCTGTTGCCGAAAGGCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAAGTCATCCGCATTTGCAACCTCGAAGGCCGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTGCCGGACACCACTCGCAAGAAATTGATGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCACCGTGATGACATGATTACCCGTTAAGGAGCAGGCTGATGCTGGAACAAATGGGCATTGCCGCGAAGCAAGCCTCGTATAA  >  NC_000913/261263‑261546

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑