Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,780,330	G→A	G799G (GGC→GGT)	ypjA ←	adhesin‑like autotransporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,780,330	0	G	A	100.0%	109.1 / NA	29	G799G (GGC→GGT)	ypjA	adhesin‑like autotransporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/13);  total (16/13)

TTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCGTGTTCCTGTGACCCGCG  >  NC_000913/2780185‑2780469                                                                                                                                                  |                                                                                                                                            tttttATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCat                                                                                                                                        >  1:556750/1‑151 (MQ=255)   tttATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACCCCCTCGACAATTTGTTCCCCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCata                                                                                                                                       <  1:54033/150‑1 (MQ=255)      aTTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTg                                                                                                                                    <  1:287365/150‑1 (MQ=255)                     gCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTc                                                                                                                      >  1:122823/1‑149 (MQ=255)                       tGAAACTTTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGt                                                                                                                       <  2:630048/146‑1 (MQ=255)                       tGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCag                                                                                                                    >  2:471184/1‑149 (MQ=255)                          aaCTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAagag                                                                                                               <  2:430320/151‑1 (MQ=255)                              gTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGgt                                                                                                             <  1:64339/149‑1 (MQ=255)                                 tGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCt                                                                                                         <  1:287169/150‑1 (MQ=255)                                          ccgTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACg                                                                                                <  2:442946/150‑1 (MQ=255)                                              cgtTAATTGTTGTCTCATTCGCCACCCCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGtt                                                                                             <  2:247989/149‑1 (MQ=255)                                                       ttgtCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCTGAAGAGGGGTCTGACTCCACGGTTAACACgccgcc                                                                                  >  2:334516/1‑151 (MQ=255)                                                        tgtCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACgccgcc                                                                                  >  1:587757/1‑150 (MQ=255)                                                           ctcATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCAttt                                                                              >  2:138945/1‑151 (MQ=255)                                                                    caccCCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAgg                                                                     <  2:544262/151‑1 (MQ=255)                                                                             gACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGt                                                              <  2:435948/149‑1 (MQ=255)                                                                                        cACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTg                                                 >  2:450337/1‑151 (MQ=255)                                                                                                  gAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCt                                        <  1:581490/150‑1 (MQ=255)                                                                                                             cTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGcc                            >  2:63527/1‑151 (MQ=255)                                                                                                                  gCAGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCcg                       >  1:132376/1‑151 (MQ=255)                                                                                                                    aGTGGCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCcgc                      >  1:271143/1‑150 (MQ=255)                                                                                                                       ggCTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCgt                  <  2:366141/151‑1 (MQ=255)                                                                                                                         cTTTTGTTTTGACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCgtgt                >  2:172966/1‑151 (MQ=255)                                                                                                                           tttgttttgACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCGTGTTc              >  1:25959/1‑151 (MQ=255)                                                                                                                           tttgttttgACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCGTGTTc              >  2:509037/1‑151 (MQ=255)                                                                                                                              gttttgACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCGTGTTCCtg           >  1:207383/1‑151 (MQ=255)                                                                                                                                   gACGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCGTGTTCCTGTGAcc      >  2:213197/1‑151 (MQ=255)                                                                                                                                     cGATCTCCCGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCGTGTTCCTGTGACCcg    >  2:173860/1‑151 (MQ=255)                                                                                                                                           cccGTCCACCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCGTGTTCCTGTGACCcgcg  <  1:104894/147‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                            TTTTTATTGCCTCTCCGTTAGCTGAAACTGTTTGTATTCCGCCGTCGTTAATTGTTGTCTCATTCGCCACACCCTCGACAATTTGTTCACCGCCGGTGAGCGTCGTGCCTGTCGCAGTGGCTTTTGTTTTGACGATCTCCCGTCCGCCCATATTGACCTGTGTTTTGTCAGAAGAGGTGTCTGACTCCACGGTTAACACGCCGCCATTTGCCAGCAGGATATTGTTCGCCGCACCCTGCTCGATGCTGAACGCGACGCCATCCGCGCGTGTTCCTGTGACCCGCG  >  NC_000913/2780185‑2780469

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑