| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,803,836 | T→C | L112P (CTA→CCA) | nrdF → | ribonucleoside‑diphosphate reductase 2, beta subunit, ferritin‑like protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,803,836 | 0 | T | C | 97.4% | 136.7 / ‑3.9 | 39 | L112P (CTA→CCA) | nrdF | ribonucleoside‑diphosphate reductase 2, beta subunit, ferritin‑like protein |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (17/21); total (17/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.60e-01 | |||||||||||
ACGCTGCTCGACACGCTGCAAAATGTTATCGGCGCGCCTTCTCTGATGCCCGATGCACTCACGCCTCATGAAGAAGCGGTATTATCGAATATCAGCTTTATGGAAGCGGTTCATGCCCGCTCTTACAGTTCGATTTTCTCGACGCTATGCCAGACCAAAGATGTCGATGCCGCCTACGCCTGGAGTGAAGAAAACGCACCGTTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGTGTTTCTTGAATCTTT > NC_000913/2803691‑2803980 | aCGCTGCTCGACACGCTGCAAAATGTTATCGGCGCGCCTTCTCTGATGCCCGATGCACTCACGCCTCATGAAGAAGCGGTATTATCGAATATCAGCTTTATGGAAGCGGTTCATGCCCGCTCTTACAGTTCGATTTTCTCGACGCCATGcc > 1:173979/1‑151 (MQ=255) gctCGACACGCTGCAAAATGTTATCGGCGCGCCTTCTCTGATGCCCGATGCACTCACGCCTCATGAAGAAGCGGTATTATCGAATATCAGCTTTATGGAAGCGGTTCATGCCCGCTCTTACAGTTCGATTTTCTCGACGCCATGCCAGAcc > 2:516483/1‑151 (MQ=255) cGACACGCTGCAAAATGTTATCGGCGCGCCTTCTCTGATGCCCGATGCACTCACGCCTCATGAAGAAGCGGTATTATCGAATATCAGCTTTATGGAAGCGGTTCATGCCCGCTCTTACAGTTCGATTTTCTCGACGCCATGCCAGACCaa < 2:472842/150‑1 (MQ=255) acacGCTGCAAAATGTTATCGGCGCGCCTTCTCTGATGCCCGATGCACTCACGCCTCATGAAGAAGCGGTATTATCGAATATCAGCTTTATGGAAGCGGTTCATGCCCGCTCTTACAGTTCGATTTTCTCGACGCCATGCCAGACCAAAGa < 2:181207/151‑1 (MQ=255) cGCTGCAAAATGTTATCGGCGCGCCTTCTCTGATGCCCGATGCACTCACGCCTCATGAAGAAGCGGTATTATCGAATATCAGCTTTATGGAAGCGGTTCATGCCCGCTCTTACAGTTCGATTTTCTCGACGCCATGCCAGACCAAAGATGt > 1:302391/1‑151 (MQ=255) cAAAATGTTATCGGCGCGCCTTCTCTGATGCCCGATGCACTCACGCCTCATGAAGAAGCGGTATTATCGAATATCAGCTTTATGGAAGCGGTTCATGCCCGCTCTTACAGTTCGATTTTCTCGACGCCATGCCAGACCAAAGATGTCGATg < 2:81337/151‑1 (MQ=255) cgcgcCTTCTCTGATGCCCGATGCACTCACGCCTCATGAAGAAGCGGTATTATCGAATATCAGCTTTATGGAAGCGGTTCATGCCCGCTCTTACAGTTCGATTTTCTCGACGCCATGCCAGACCAAAGATGTCGATGCCGCCTACGCCTgg > 1:77311/1‑151 (MQ=255) ttCTCTGATGCCCGATGCACTCACGCCTCATGAAGAAGCGGTATTATCGAATATCAGCTTTATGGAAGCGGTTCATGCCCGCTCTTACAGTTCGATTTTCTCGACGCCATGCCAGACCAAAGATGTCGATGCCGCCTACGCCTGGAGTga > 2:24696/1‑150 (MQ=255) cGATGCACTCACGCCTCATGAAGAAGCGGTATTATCGAATATCAGCTTTATGGAAGCGGTTCATGCCCGCTCTTACAGTTCGATTTTCTCGACGCCATGCCAGACCAAAGATGTCGATGCCGCCTACGCCTGGAGTGAAGAAAACGCACCg < 1:150421/151‑1 (MQ=255) gATGCACTCACGCCTCATGAAGAAGCGGTATTATCGAATATCAGCTTTATGGAAGCGGTTCATGCCCGCTCTTACAGTTCGATTTTCTCGACGCCATGCCAGACCAAAGATGTCGATGCCGCCTACGCCTGGAGTGAAGAAAACGCACCGt > 2:200777/1‑151 (MQ=255) tGCACTCTCGCCTCATGAAGAAGCGGTATTATCGAATATCAGCTTTATGGAAGCGGTTCATGCCCGCTCTTACAGTTCGATTTTCTCGACGCCATGCCAGACCTAAGATGTCGATGCCGCCTACGCCTGGAGTGTAGAAAACGCAc < 2:515081/146‑1 (MQ=255) cACGCCTCATGAAGAAGCGGTATTATCGAATATCAGCTTTATGGAAGCGGTTCATGCCCGCTCTTACAGTTCGATTTTCTCGACGCCATGCCAGACCAAAGATGTCGATGCCGCCTACGCCTGGAGTGAAGAAAACGCACCGTTGCAGCg < 2:612726/150‑1 (MQ=255) cATGAAGAAGCGGTATTATCGAATATCAGCTTTATGGAAGCGGTTCATGCCCGCTCTTACAGTTCGATTTTCTCGACGCCATGCCAGACCAAAGATGTCGATGCCGCCTACGCCTGGAGTGAAGAAAACGCACCGTTGCAGCGAAAAGCTc < 2:96870/151‑1 (MQ=255) tGAAGAAGCGGTATTATCGAATATCAGCTTTATGGAAGCGGTTCATGCCCGCTCTTACAGTTCGATTTTCTCGACGCCATGCCAGACCAAAGATGTCGATGCCGCCTACGCCTGGAGTGAAGAAAACGCACCGTTGCAGCGAAAAGCTc > 1:399915/1‑149 (MQ=255) gaagCGGTATTATCGAATATCAGCTTTATGGAAGCGGTTCATGCCCGCTCTTACAGTTCGATTTTCTCGACGCCATGCCAGACCAAAGATGTCGATGCCGCCTACGCCTGGAGTGAAGAAAACGCACCGTTGCAGCGAAAAGCTCAGatta < 1:267592/151‑1 (MQ=255) aagCGGTATTATCGAATATCAGCTTTATGGAAGCGGTTCATGCCCGCTCTTACAGTTCGATTTTCTCGACGCCATGCCAGACCAAAGATGTCGATGCCGCCTACGCCTGGAGTGAAGAAAACGCACCGTTGCAGCGAAAAGCTCAGattat > 1:515410/1‑151 (MQ=255) ggTATTATCGAATATCAGCTTTATGGAAGCGGTTCATGCCCGCTCTTACAGTTCGATTTTCTCGACGCCATGCCAGACCAAAGATGTCGATGCCGCCTACGCCTGGAGTGAAGAAAACGCACCGTTGCAGCGAAAAGCTCAGATTATTc > 2:298099/1‑149 (MQ=255) tattatCGAATATCAGCTTTATGGAAGCGGTTCATGCCCGCTCTTACAGTTCGATTTTCTCGACGCCATGCCAGACCAAAGATGTCGATGCCGCCTACGCCTGGAGTGAAGAAAACGCACCGTTGCAGCGAAAAGCTCAGATTATTCAGc > 1:583293/1‑150 (MQ=255) attatCGAATATCAGCTTTATGGAAGCGGTTCATGCCCGCTCTTACAGTTCGATTTTCTCGACGCCATGCCAGACCAAAGATGTCGATGCCGCCTACGCCTGGAGTGAAGAAAACGCACCGTTGCAGCGAAAAGCTCAGATTATTCAGCa < 1:79888/150‑1 (MQ=255) attatCGAATATCAGCTTTATGGAAGCGGTTCATGCCCGCTCTTACAGTTCGATTTTCTCGACGCCATGCCAGACCAAAGATGTCGATGCCGCCTACGCCTGGAGTGAAGAAAACGCACCGTTGCAGCGAAAAGCTCAGATTATTCAGCa > 2:240237/1‑150 (MQ=255) atCGAATATCAGCTTTATGGAAGCGGTTCATGCCCGCTCTTACAGTTCGATTTTCTCGACGCCATGCCAGACCAAAGATGTCGATGCCGCCTACGCCTGGAGTGAAGAAAACGCACCGTTGCAGCGAAAAGCTCAGATTATTCAGCAACAt > 2:281264/1‑151 (MQ=255) atCGAATATCAGCTTTACGGAAGCGGTTCATGCCCGCTCTTACAGTTCGATTTTCTCGACGCTATGCCAGACCAAAGATGTCGATGCCGACTACGCCTGGAGTGAAGAAAACGCACCGTTGCAGCGAAAAGCTCAGATTATTCAGCAACAt < 2:431031/151‑1 (MQ=255) cGAATATCAGCTTTATGGAAGCGGTTCATGCCCGCTCTTACAGTTCGATTTTCTCGACGCCATGCCAGACCAAAGATGTCGATGCCGCCTACGCCTGGAGTGAAGAAAACGCACCGTTGCAGCGAAAAGCTCAGATTATTCAGCAACAtt < 1:22634/150‑1 (MQ=255) atatCAGCTTTATGGAAGCGGTTCATGCCCGCTCTTACAGTTCGATTTTCTCGACGCCATGCCAGACCAAAGATGTCGATGCCGCCTACGCCTGGAGTGAAGAAAACGCACCGTTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATcg < 1:244116/151‑1 (MQ=255) tCAGCTTTATGGAAGCGGTTCATGCCCGCTCTTACAGTTCGATTTTCTCGACGCCATGCCAGACCAAAGATGTCGATGCCGCCTACGCCTGGAGTGAAGAAAACGCACCGTTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCgg > 1:211120/1‑151 (MQ=255) cAGCTTTATGGAAGCGGTTCATGCCCGCTCTTACAGTTCGATTTTCTCGACGCCATGCCAGACCAAAGATGTCGATGCCGCCTACGCCTGGAGTGAAGAAAACGCACCGTTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGt > 1:331342/1‑151 (MQ=255) ttATGGAAGCGGTTCATGCCCGCTCTTACAGTTCGATTTTCTCGACGCCATGCCAGACCAAAGATGTCGATGCCGCCTACGCCTGGAGTGAAGAAAACGCACCGTTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGtgatg < 1:73727/150‑1 (MQ=255) ttATGGAAGCGGTTCATGCCCGCTCTTACAGTTCGATTTTCTCGACGCCATGCCAGACCAAAGATGTCGATGCCGCCTACGCCTGGAGTGAAGAAAACGCACCGTTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGtgatg < 1:266017/150‑1 (MQ=255) tATGGAAGCGGTTCATGCCCGCTCTTACAGTTCGATTTTCTCGACGCCATGCCAGACCAAAGATGTCGATGCCGCCTACGCCTGGAGTGAAGAAAACGCACCGTTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGtgatga < 2:576757/150‑1 (MQ=255) tATGGAAGCGGTTCATGCCCGCTCTTACAGTTCGATTTTCTCGACGCCATGCCAGACCAAAGATGTCGATGCCGCCTACGCCTGGAGTGAAGAAAACGCACCGTTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGtgatga < 2:241199/150‑1 (MQ=255) aTGGAAGCGGTTCATGCCCGCTCTTACAGTTCGATTTTCTCGACGCCATGCCAGACCAAAGATGTCGATGCCGCCTACGCCTGGAGTGAAGAAAACGCACCGTTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATc < 1:139216/151‑1 (MQ=255) gCGGTTCATGCCCGCTCTTACAGTTCGATTTTCTCGACGCCATGCCAGACCAAAGATGTCGATGCCGCCTACGCCTGGAGTGAAGAAAACGCACCGTTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTg > 1:553635/1‑150 (MQ=255) ttCATGCCCGCTCTTACAGTTCGATTTTCTCGACGCCATGCCAGACCAAAGATGTCGATGCCGCCTACGCCTGGAGTGAAGAAAACGCACCGTTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGaaaa > 1:381181/1‑150 (MQ=255) gCCCGCTCTTACAGTTCGATTTTCTCGACGCCATGCCAGACCAAAGATGTCGATGCCGCCTACGCCTGGAGTGAAGAAAACGCACCGTTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGaaaa < 1:525023/151‑1 (MQ=255) cccGCTCTTACAGTTCGATTTTCTCGACGCCATGCCAGACCAAAGATGTCGATGCCGCCTACGCCTGGAGTGAAGAAAACGCACCGTTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAAt < 1:616450/151‑1 (MQ=255) tACAGTTCGATTTTCTCGACGCCATGCCAGACCAAAGATGTCGATGCCGCCTACGCCTGGAGTGAAGAAAACGCACCGTTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCa < 1:273393/148‑1 (MQ=255) cGATTTTCTCGACGCCATGCCAGACCAAAGATGTCGATGCCGCCTACGCCTGGAGTGAAGAAAACGCACCGTTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGTGTTTCt > 1:3191/1‑151 (MQ=255) cGACGCCATGCCAGACCAAAGATGTCGATGCCGCCTACGCCTGGAGTGAAGAAAACGCACCGTTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGTGTTTCTTGAATCttt < 1:503669/151‑1 (MQ=255) cGACGCCATGCCAGACCAAAGATGTCGATGCCGCCTACGCCTGGAGTGAAGAAAACGCACCGTTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGTGTTTCTTGAATCttt < 1:103506/151‑1 (MQ=255) | ACGCTGCTCGACACGCTGCAAAATGTTATCGGCGCGCCTTCTCTGATGCCCGATGCACTCACGCCTCATGAAGAAGCGGTATTATCGAATATCAGCTTTATGGAAGCGGTTCATGCCCGCTCTTACAGTTCGATTTTCTCGACGCTATGCCAGACCAAAGATGTCGATGCCGCCTACGCCTGGAGTGAAGAAAACGCACCGTTGCAGCGAAAAGCTCAGATTATTCAGCAACATTATCGCGGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGTGTTTCTTGAATCTTT > NC_000913/2803691‑2803980 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |