Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,978,727	T→C	N58S (AAT→AGT)	lysA ←	diaminopimelate decarboxylase, PLP‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,978,727	0	T	C	100.0%	144.2 / NA	38	N58S (AAT→AGT)	lysA	diaminopimelate decarboxylase, PLP‑binding
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/16);  total (22/16)

CCTGATCGATAACATCTGCCGTAAAAACAATATCATCGGGGTGCGTTTGCGGATTGTAACCCGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATATTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTGCGCATCGTAGACCCACACCGGGCAGCCAAATTCAGCGGGCAAACGCAGCAGATTTTCGGCGGTGAGATCG  >  NC_000913/2978581‑2978870                                                                                                                                                   |                                                                                                                                                ccTGATCGATAACATCTGCCGTAAAAACAATATCATCGGGGTGCGTTTGCGGATTGTAACCCGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGa                                                                                                                                             <  2:350743/151‑1 (MQ=255)      tCGATAACATCTGCCGTAAAAACAATATCATCGGGGTGCGTTTGCGGATTGTAACCCGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAgg                                                                                                                                        >  1:76474/1‑151 (MQ=255)       cGATAACATCTGCCGTAAAAACAATATCATCGGGGTGCGTTTGCGGATTGTAACCCGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGc                                                                                                                                       >  1:155987/1‑151 (MQ=255)          tAACATCTGCCGTAAAAACAATATCATCGGGGTGCGTTTGCGGATTGTAACCCGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCttt                                                                                                                                    >  2:343986/1‑151 (MQ=255)               tCTGCCGTAAAAACAATATCATCGGGGTGCGTTTGCGGATTGTAACCCGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCtgtg                                                                                                                               >  2:223112/1‑151 (MQ=255)                       aaaaaCAATATCATCGGGGTGCGTTTGCGGATTGTAACCCGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAgcg                                                                                                                        <  1:267032/150‑1 (MQ=255)                        aaaaCAATATCATCGGGGTGCGTTTGCGGATTGTAACCCGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAgcgc                                                                                                                       >  2:26442/1‑150 (MQ=255)                         aaaCAATGTCATCGGGGTGCTTTTGCGGATTGTAACCCGCCGCCAACGCACGCTCTATTTCGCCTATCGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATCTGAATACTGTAACAGGCTTTCTGTGCAAAgcg                                                                                                                        <  2:459343/148‑1 (MQ=255)                                   atcGGGGTGCGTTTGCGGATTGTAACCCGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAAc                                                                                                           >  1:58320/1‑151 (MQ=255)                                     cGGGGTGCGTTTGCGGATTGTAACCCGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTg                                                                                                         >  2:399986/1‑151 (MQ=255)                                            cGTTTGCGGATTGTAACCCGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAgc                                                                                                  <  1:110369/151‑1 (MQ=255)                                                tGCGGATTGTAACCCGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCt                                                                                               <  2:178177/150‑1 (MQ=255)                                                  cGGATTGTAACCCGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCa                                                                                            <  2:433770/151‑1 (MQ=255)                                                   ggATTGTAACCCGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCa                                                                                            >  1:531932/1‑150 (MQ=255)                                                        gTAACCCGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTg                                                                                       <  1:223117/150‑1 (MQ=255)                                                            cccGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCcgac                                                                                  >  2:568195/1‑151 (MQ=255)                                                             ccgccgCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCcgacg                                                                                 >  1:463134/1‑151 (MQ=255)                                                                         cacgcTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTgcgc                                                                     >  2:361636/1‑151 (MQ=255)                                                                          acgcTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTgcgc                                                                     >  1:166732/1‑150 (MQ=255)                                                                           cgcTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTGCGCAt                                                                   <  1:263728/151‑1 (MQ=255)                                                                                      gCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTGCGCATCGTAGACCcac                                                        >  1:509324/1‑151 (MQ=255)                                                                                          aaCGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTGCGCATCGTAGACCCacac                                                      <  1:623641/149‑1 (MQ=255)                                                                                           aCGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCGCCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTGCGCATCGTAGACCCACACCgg                                                   >  1:602532/1‑151 (MQ=255)                                                                                           aCGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTGCGCATCGTAGACCCACACCgg                                                   <  2:28124/151‑1 (MQ=255)                                                                                                 cGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTGCGCATCGTAGACCCACACCGGGCAGcc                                             <  1:454280/151‑1 (MQ=255)                                                                                                   gAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTGCGCATCGTAGACCCACACCGGGCAGCCa                                            >  1:559473/1‑150 (MQ=255)                                                                                                             tCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTGCGCATCGTAGACCCACACCGGGCAGCCAAATTCAGCggg                                 >  1:352627/1‑151 (MQ=255)                                                                                                              cACGCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTGCGCATCGTAGACCCACACCGGGCAGCCAAATTCAGCggg                                 <  2:536755/150‑1 (MQ=255)                                                                                                                 gCCCTGCTCACGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTGCGCATCGTAGACCCACACCGGGCAGCCAAATTCAGCGGGCaaa                             <  2:506097/151‑1 (MQ=255)                                                                                                                           cGCATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTGCGCATCGTAGACCCACACCGGGCAGCCAAATTCAGCGGGCAAACGCAGCAGa                    <  2:419452/150‑1 (MQ=255)                                                                                                                             cATTAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTGCGCATCGTAGACCCACACCGGGCAGCCAAATTCAGCGGGCAAACGCAGCAGAttt                 >  2:302932/1‑151 (MQ=255)                                                                                                                               ttAAGCGCAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTGCGCATCGTAGACCCACACCGGGCAGCCAAATTCAGCGGGCAAACGCAGCAGATTTTc               >  1:76448/1‑151 (MQ=255)                                                                                                                                      cAAAATATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTGCGCATCGTAGACCCACACCGGGCAGCCAAATTCAGCGGGCAAACGCAGCAGATTTTCGGCGGTg        <  2:306525/151‑1 (MQ=255)                                                                                                                                         aaTATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTGCGCATCGTAGACCCACACCGGGCAGCCAAATTCAGCGGGCAAACGCAGCAGATTTTCGGCGGTg        >  1:453540/1‑148 (MQ=255)                                                                                                                                         aaTATGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTGCGCATCGTAGACCCACACCGGGCAGCCAAATTCAGCGGGCAAACGCAGCAGATTTTCGGCGGTg        >  1:462910/1‑148 (MQ=255)                                                                                                                                           tatGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTGCGCATCGTAGACCCACACCGGGCAGCCAAATTCAGCGGGCAAACGCAGCAGATTTTCGGCGGTg        >  1:181364/1‑146 (MQ=255)                                                                                                                                            atGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTGCGCATCGTAGACCCACACCGGGCAGCCAAATTCAGCGGGCAAACGCAGCAGATTTTCGGCGGTGAGatcg  <  2:446526/151‑1 (MQ=255)                                                                                                                                             tGAATACTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTGCGCATCGTAGACCCACACCGGGCAGCCAAATTCAGCGGGCAAACGCAGCAGATTTTCGGCGGTg        >  1:201928/1‑144 (MQ=255)                                                                                                                                                   |                                                                                                                                                CCTGATCGATAACATCTGCCGTAAAAACAATATCATCGGGGTGCGTTTGCGGATTGTAACCCGCCGCCAACGCACGCTCTATTTCGCCTAACGAGACGGAATCCACTTTCACGCCCTGCTCACGCATTAAGCGCAAAATATGAATATTGGAACAGGCTTTCTGTGCAAAGCGCACCACATCAAACTGTTTCAGCGCTGCAATCTGCCGACGAATAATTTGCGCATCGTAGACCCACACCGGGCAGCCAAATTCAGCGGGCAAACGCAGCAGATTTTCGGCGGTGAGATCG  >  NC_000913/2978581‑2978870

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑