Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	277,958	A→G	L282P (CTC→CCC)	afuC ←	CP4‑6 prophage; putative ferric transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	277,958	0	A	G	100.0%	126.4 / NA	37	L282P (CTC→CCC)	afuC	CP4‑6 prophage; putative ferric transporter subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/20);  total (17/20)

CTGCTCGCCGACGTCCGGTTGCAGACGCGTAGCGTTGACCTGCAATAATATCTCCTGCCCGTGCCATTCCACCGTCACTTCATACTGCGGCCCCATATAGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGAGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCACCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAGATATCAACGTATCCGTCGCTGAAGGTTGCCGGGAACAGGTTGGCATCGCCCATAA  >  NC_000913/277810‑278096                                                                                                                                                     |                                                                                                                                           cTGCTCGCCGACGTCCGGTTGCAGACGCGTAGCGTTGACCTGCAATAATATCTCCTGCCCGTGCCATTCCACCGTCACTTCATACTGCGGCCCCATATAGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGc                                                                                                                                          >  2:464274/1‑151 (MQ=255)   gCTCGCCGACGTCCGGTTGCAGACGCGTAGCGTTGACCTGCAATAATATCTCCTGCCCGTGCCATTCCACCGTCACTTCATACTGCGGCCCCATATAGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGc                                                                                                                                          <  1:562401/149‑1 (MQ=255)      cGCCGACGTCCGGTTGCAGACGCGTAGCGTTGACCTGCAATAATATCTCCTGCCCGTGCCATTCCACCGTCACTTCATACTGCGGCCCCATATAGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGAt                                                                                                                                     >  2:503006/1‑151 (MQ=255)       gCCGACGTCCGGTTGCAGACGCGTAGCGTTGACCTGCAATAATATCTCCTGCCCGTGCCATTCCACCGTCACTTCATACTGCGGCCCCATATAGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATc                                                                                                                                    >  1:243474/1‑151 (MQ=255)       gCCGACGTCCGGTTGCAGACGCGTAGCGTTGACCTGCAATAATATCTCCTGCCCGTGCCATTCCACCGTCACTTCATACTGCGGCCCCATATAGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATc                                                                                                                                    <  1:458090/151‑1 (MQ=255)           aCGTCCGGTTGCAGACGCGTAGCGTTGACCTGCAATAATATCTCCTGCCCGTGCCATTCCACCGTCACTTCATACTGCGGCCCCATATAGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCtt                                                                                                                                >  1:520311/1‑151 (MQ=255)                 ggTTGCAGACGCGTAGCGTTGACCTGCAATAATATCTCCTGCCCGTGCCATTCCACCGTCACTTCATACTGCGGCCCCATATAGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGgc                                                                                                                          >  2:206747/1‑151 (MQ=255)                       aGACGCGTAGCGTTGACCTGCAATAATATCTCCTGCCCGTGCCATTCCACCGTCACTTCATACTGCGGCCCCATATAGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCacac                                                                                                                    <  2:472707/151‑1 (MQ=255)                        gACGCGTAGCGTTGACCTGCAATAATATCTCCTGCCCGTGCCATTCCACCGTCACTTCATACTGCGGCCCCATATAGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACAcc                                                                                                                   >  1:435646/1‑151 (MQ=255)                                 cGTTGACCTGCAATAATATCTCCTGCCCGTGCCATTCCACCGTCACTTCATACTGCGGCCCCGTATAGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATccc                                                                                                          <  1:155747/151‑1 (MQ=255)                                 cGTTGACCTGCAATAATATCTCCTGCCCGTGCCATTCCACCGTCACTTCATACTGCGGCCCCATATAGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATccc                                                                                                          <  1:39051/151‑1 (MQ=255)                                        cTGCAATAATATCTCCTGCCCGTGCCATTCCACCGTCACTTCATACTGCGGCCCCATATAGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGccc                                                                                                   >  1:98323/1‑151 (MQ=255)                                           cAATAATATCTCCTGCCCGTGCCATTCCACCGTCACTTCATACTGCGGCCCCATATAGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCtgt                                                                                                <  1:265317/151‑1 (MQ=255)                                           cAATAATATCTCCTGCCCGTGCCATTCCACCGTCACTTCATACTGCGGCCCCATATAGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCtgt                                                                                                >  1:333350/1‑151 (MQ=255)                                               aataTCTCCTGCCCGTGCCATTCCACCGTCACTTCATACTGCGGCCCCATATAGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTAc                                                                                            >  2:534676/1‑151 (MQ=255)                                                               gCCATTCCACCGTCACTTCATACTGCGGCCCCATATAGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGgcg                                                                            >  1:283822/1‑151 (MQ=255)                                                               gCCATTCCACCGTCACTTCATACTGCGGCCCCATATAGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGgc                                                                             <  2:97286/150‑1 (MQ=255)                                                                    tCCACCGTCACTTCATACTGCGGCCCCATATAGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGc                                                                        >  2:36591/1‑150 (MQ=255)                                                                       aCCGTCACTTCATACTGCGGCCCCATATAGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGc                                                                        >  1:177288/1‑147 (MQ=255)                                                                              cTTCATACTGCGGCCCCATATATGCGACATGGCGGGTCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACCCCGACCATCCCATCGCCCTGTGTACCAAAGTGCAGCGGGCGCTGCAGAGGATa                                                                <  2:610357/148‑1 (MQ=255)                                                                                tCATACTGCGGCCCCATATAGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCg                                                            <  1:493971/150‑1 (MQ=255)                                                                                  aTACTGCGGCCCCATATAGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAg                                                         <  2:133143/151‑1 (MQ=255)                                                                                      tGCGGCCCCATATAGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAGatat                                                     >  2:130724/1‑151 (MQ=255)                                                                                          gCCCCATATAGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAGATATCAAc                                                 <  1:213766/151‑1 (MQ=255)                                                                                                tataGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAGATATCAACGTATcc                                           >  2:50380/1‑151 (MQ=255)                                                                                                  taGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAGATATCAACGTATCCGt                                         >  2:98569/1‑151 (MQ=255)                                                                                                      cGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTATCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAGATATCAACGTATCCGTCGCt                                     <  1:226646/151‑1 (MQ=255)                                                                                                       gACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAGATATCAACGTATCCGTCGCTg                                    <  2:338362/151‑1 (MQ=255)                                                                                                          aTGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAGATATCAACGTATCCGTCGCTGAAg                                 <  2:65459/151‑1 (MQ=255)                                                                                                          aTGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAGATATCAACGTATCCGTCGCTGAAg                                 <  1:176271/151‑1 (MQ=255)                                                                                                          aTGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAGATATCAACGTATCCGTCGCTGAAg                                 <  1:406005/151‑1 (MQ=255)                                                                                                                gATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAGATATCAACGTATCCGTCGCTGAAGGTTGcc                           <  1:98569/151‑1 (MQ=255)                                                                                                                 aTCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAGATATCAACGTATCCGTCGCTGAAGGTTGCCg                          >  1:615280/1‑151 (MQ=255)                                                                                                                    aCGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAGATGTCAACGTATCCGTCGCTGAAGGTTGCCGGGa                       <  1:572949/151‑1 (MQ=255)                                                                                                                      gCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAGATATCAACGTATCCGTCGCTGAAGGTTGCCGGGAAc                     <  1:8124/151‑1 (MQ=255)                                                                                                                              ggCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAGATATCAACGTATCCGTCGCTGAAGGTTGCCGGGAACAGGTTg               <  2:572740/149‑1 (MQ=255)                                                                                                                                           gcgATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAGATATCAACGTATCCGTCGCTGAAGGTTGCCGGGAACAGGTTGGCATCGCCCATaa  >  1:73038/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                           CTGCTCGCCGACGTCCGGTTGCAGACGCGTAGCGTTGACCTGCAATAATATCTCCTGCCCGTGCCATTCCACCGTCACTTCATACTGCGGCCCCATATAGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGAGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCACCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAGATATCAACGTATCCGTCGCTGAAGGTTGCCGGGAACAGGTTGGCATCGCCCATAA  >  NC_000913/277810‑278096

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑