Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,018,435	A→G	E792E (GAA→GAG)	ygfK →	putative Fe‑S subunit oxidoreductase subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,018,435	0	A	G	100.0%	146.4 / NA	39	E792E (GAA→GAG)	ygfK	putative Fe‑S subunit oxidoreductase subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/18);  total (21/18)

GATGCTGATGGCACCTTAACCTTGCGCGTTATGTCGCTTGGCGAACCGGATGAGAAAGGTCGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAAGCCCTGAATGCGATGGGCGTGCCGCTGGACAAAAACGGCTGGCCAGACGTCGACCATAATGGCGAAACTCGTCTGACTGACGTCTTTATGATCGGCGACGTACAGCGCGGACCATCCTCCATTGTCGCTGCTGTCGGAACC  >  NC_000913/3018289‑3018576                                                                                                                                                   |                                                                                                                                              gATGCTGATGGCACCTTAACCTTGCGCGTTATGTCGCTTGGCGAACCGGATGAGAAAGGTCGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAGGccc                                                                                                                                           >  1:70554/1‑151 (MQ=255)          ggCACCTTAACCTTGCGCGTTATGTCGCTTGGCGAACCGGATGAGAAAGGTCGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAGGCCCTGAATGCg                                                                                                                                   <  1:50369/150‑1 (MQ=255)           gCACCTTAACCTTGCGCGTTATGTCGCTTGGCGAACCGGATGAGAAAGGTCGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAGGCCCTGAATGCGAt                                                                                                                                 <  2:58240/151‑1 (MQ=255)                   aCCTTGCGCGTTATGTCGCTTGGCGAACCGGATGAGAAAGGTCGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAGGCCCTGAATGCGATGGGCGTGc                                                                                                                         >  2:80788/1‑151 (MQ=255)                               aTGTCGCTTGGCGAACCGGATGAGAAAGGTCGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAGGCCCTGAATGCGATGGGCGTGCCGCTGGACaaaa                                                                                                             <  2:486552/151‑1 (MQ=255)                                   cGCTTGGCGAACCGGATGAGAAAGGTCGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAGGCCCTGAATGCGATGGGCGTGCCGCTGGACAAAAACgg                                                                                                         >  1:51015/1‑151 (MQ=255)                                       tGGCGAACCGGATGAGAAAGGTCGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAGGCCCTGAATGCGATGGGCGTGCCGCTGGACAAAAACGGCTg                                                                                                      <  2:415170/150‑1 (MQ=255)                                         gCGAACCGGATGAGAAAGGTCGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAGGCCCTGAATGCGATGGGCGTGCCGCTGGACAAAAACGGCTGGcc                                                                                                   <  1:620064/151‑1 (MQ=255)                                            aaCCGGATGAGAAAGGTCGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAGGCCCTGAATGCGATGGGCGTGCCGCTGGACAAAAACGGCTGGcc                                                                                                   >  1:123163/1‑148 (MQ=255)                                               cGGATGAGAAAGGTCGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAGGCCCTGAATGCGATGGGCGTGCCGCTGGACAAAAACGGCTGGCCAGACGt                                                                                             <  2:70554/151‑1 (MQ=255)                                                ggATGAGAAAGGTCGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAGGCCCTGAATGCGATGGGCGTGCCGCTGGACAAAAACGGCTGGCCAGACGTc                                                                                            <  2:55593/151‑1 (MQ=255)                                                   tGAGAAAGGTCGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAGGCCCTGAATGCGATGGGCGTGCCGCTGGACAAAAACGGCTGGCCAGACGTCGAc                                                                                         >  2:610902/1‑151 (MQ=255)                                                    gagaAAGGTCGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAGGCCCTGAATGCGATGGGCGTGCCGCTGGACAAAAACGGCTGGCCAGACGTCGAc                                                                                         >  2:265764/1‑150 (MQ=255)                                                       aaaGGTCGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAGGCCCTGAATGCGATGGGCGTGCCGCTGGACAAAAACGGCTGGCCAGACGTCGACCATa                                                                                     <  1:332889/151‑1 (MQ=255)                                                         aGGTCGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAGGCCCTGAATGCGATGGGCGTGCCGCTGGACAAAAACGGCTGGCCAGACGTCGACCATAAt                                                                                   >  1:417741/1‑151 (MQ=255)                                                                cgtcgtcCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAGGCCCTGAATGCGATGGGCGTGCCGCTGGACAAAAACGGCTGGCCAGACGTCGACCATAATGGCGaa                                                                             <  1:166644/150‑1 (MQ=255)                                                                    gtcCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAGGCCCTGAATGCGATGGGCGTGCCGCTGGACAAAAACGGCTGGCCAGACGTCGACCATAATGGCGAAACTCg                                                                        <  2:315418/151‑1 (MQ=255)                                                                     tcCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCGTGATCACCGCCATTGGTGAACAGCAGGATACTGAGGCCCTGAATGCGATGGGCGTGCCGCTGGACAAAAACGGCTGGCCAGACGTCGACCATAATGGCGAAACTCGt                                                                       >  2:356576/1‑151 (MQ=255)                                                                      ccGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAGGCCCTGAATGCGATGGGCGTGCCGCTGGACAAAAACGGCTGGCCAGACGTCGACCATAATGGCGAAACTCGTc                                                                      >  2:534146/1‑151 (MQ=255)                                                                       cGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAGGCCCTGAATGCGATGGGCGTGCCGCTGGACAAAAACGGCTGGCCAGACGTCGACCATAATGGAGAAACTCGTc                                                                      >  2:444144/1‑150 (MQ=255)                                                                        ggTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAGGCCCTGAATGCGATGGGCGTGCCGCTGGACAAAAACGGCTGGCCAGACGTCGACCATAATGGCGAAACTCGTctg                                                                    >  1:328675/1‑151 (MQ=255)                                                                        ggTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAGGCCCTGAATGCGATGGGCGTGCCGCTGGACAAAAACGGCTGGCCAGACGTCGACCATAATGGCGAAACTCGTctg                                                                    >  1:195524/1‑151 (MQ=255)                                                                             aaaCCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAGGCCCTGAATGCGATGGGCGTGCCGCTGGACAAAAACGGCTGGCCAGACGTCGACCATAATGGCGAAACTCGTctgactg                                                                >  2:541690/1‑150 (MQ=255)                                                                                ccAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAGGCCCTGAATGCGATGGGCGTGCCGCTGGACAAAAACGGCTGGCCAGACGTCGACCATAATGGCGAAACTCGTCTGACTGACg                                                             <  2:185993/150‑1 (MQ=255)                                                                                      aaaCAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAGGCCCTGAATGCGATGGGCGTGCCGCTGGACAAAAACGGCTGGCCAGACGTCGACCATAATGGCGAAACTCGTCTGACTGACGTCTTTAt                                                      <  2:394619/151‑1 (MQ=255)                                                                                           gTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAGGCCCTGAATGCGATGGGCGTGCCGCTGGACAAAAACGGCTGGCCAGACGTCGACCATAATGGCGAAACTCGTCTGACTGACGTCTTTATGATCg                                                 <  1:224399/151‑1 (MQ=255)                                                                                            tAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAGGCCCTGAATGCGATGGGCGTGCCGCTGGACAAAAACGGCTGGCCAGACGTCGACCATAATGGCGAAACTCGTCTGACTGACGTCTTTATGATCg                                                 >  2:179333/1‑150 (MQ=255)                                                                                               cacTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAGGCCCTGAATGCGATGGGCGTGCCGCTGGACAAAAACGGCTGGCCAGACGTCGACCATAATGGCGAAACTCGTCTGACTGACGTCTTTATGATCGGCg                                              >  1:415537/1‑150 (MQ=255)                                                                                                acTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAGGCCCTGAATGCGATGGGCGTGCCGCTGGACAAAAACGGCTGGCCAGACGTCGACCATAATGGCGAAACTCGTCTGACTGACGTCTTTATGATCGGCGAc                                            >  1:145866/1‑151 (MQ=255)                                                                                                     ttGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAGGCCCTGAATGCGATGGGCGTGCCGCTGGACAAAAACGGCTGGCCAGACGTCGACCATAATGGCGAAACTCGTCTGACTGACGTCTTTATGATCGGCGACGTAc                                        >  2:96145/1‑150 (MQ=255)                                                                                                       gTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAGGCCCTGAATGCGATGGGCGTGCCGCTGGACAAAAACGGCTGGCCAGACGTCGACCATAATGGCGAAACTCGTCTGACTGACGTCTTTATGATCGGCGACGTACAgc                                     <  1:594854/151‑1 (MQ=255)                                                                                                       gTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAGGCCCTGAATGCGATGGGCGTGCCGCTGGACAAAAACGGCTGGCCAGACGTCGACCATAATGGCGAAACTCGTCTGACTGACGTCTTTATGATCGGCGACGTACAg                                      <  1:10614/150‑1 (MQ=255)                                                                                                                         gCCATTGGTGAACAGCAGGATACTGAGGCCCTGAATGCGATGGGCGTGCCGCTGGACAAAAACGGCTGGCCAGACGTCGACCATAATGGCGAAACTCGTCTGACTGACGTCTTTATGATCGGCGACGTACAGCGCGGACCATCCTCCATTg                   >  2:578538/1‑151 (MQ=255)                                                                                                                          ccATTGGTGAACAGCAGGATACTGAGGCCCTGAATGCGATGGGCGTGCCGCTGGACAAAAACGGCTGGCCAGACGTCGACCATAATGGCGAAACTCGTCTGACTGACGTCTTTATGATCGGCGACGTACAGCGCGGACCATCCTCCATTGt                  <  1:270863/151‑1 (MQ=255)                                                                                                                               ggTGAACAGCAGGATACTGAGGCCCTGAATGCGATGGGCGTGCCGCTGGACAAAAACGGCTGGCCAGACGTCGACCATAATGGCGAAACTCGTCTGACTGACGTCTTTATGATCGGCGACGTACAGCGCGGACCATCCTCCATTGTCgctg             <  1:96145/151‑1 (MQ=255)                                                                                                                                 tGAACAGCAGGATACTGAGGCCCTGAATGCGATGGGCGTGCCGCTGGACAAAAACGGCTGGCCAGACGTCGACCATAATGGCGAAACTCGTCTGACTGACGTCTTTATGATCGGCGACGTACAGCGCGGACCATCCTCCATTGTCgctgct           >  2:214523/1‑151 (MQ=255)                                                                                                                                 tGAACAGCAGGATACTGAGGCCCTGAATGCGATGGGCGTGCCGCTGGACAAAAACGGCTGGCCAGACGTCGACCATAATGGCGAAACTCGTCTGACTGACGTCTTTATGATCGGCGACGTACAGCGCGGACCATCCTCCATTGTCgctgct           >  1:359255/1‑151 (MQ=255)                                                                                                                                     cagcagGATACTGAGGCCCTGAATGCGATGGGCGTGCCGCTGGACAAAAACGGCTGGCCAGACGTCGACCATAATGGCGAAACTCGTCTGACTGACGTCTTTATGATCGGCGACGTACAGCGCGGACCATCCTCCATTGTCGCTGCTGTCg       <  1:308569/151‑1 (MQ=255)                                                                                                                                          ggATACTGAGGCCCTGAATGCGATGGGCGTGCCGCTGGACAAAAACGGCTGGCCAGACGTCGACCATAATGGCGAAACTCGTCTGACTGACGTCTTTATGATCGGCGACGTACAGCGCGGACCATCCTCCATTGTCGCTGCTGTCGGAAcc  >  1:102309/1‑151 (MQ=255)                                                                                                                                                   |                                                                                                                                              GATGCTGATGGCACCTTAACCTTGCGCGTTATGTCGCTTGGCGAACCGGATGAGAAAGGTCGTCGTCGTCCGGTTGAAACCAATGAAACAGTAACACTGCTTGTAGACAGCCTGATCACCGCCATTGGTGAACAGCAGGATACTGAAGCCCTGAATGCGATGGGCGTGCCGCTGGACAAAAACGGCTGGCCAGACGTCGACCATAATGGCGAAACTCGTCTGACTGACGTCTTTATGATCGGCGACGTACAGCGCGGACCATCCTCCATTGTCGCTGCTGTCGGAACC  >  NC_000913/3018289‑3018576

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑