Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,086,618	A→G	intergenic (+216/‑88)	yqgC → / → metK	uncharacterized protein/S‑adenosylmethionine synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,086,618	0	A	G	100.0%	129.1 / NA	35	intergenic (+216/‑88)	yqgC/metK	uncharacterized protein/S‑adenosylmethionine synthetase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/18);  total (17/18)

AGGAAAAATTTCCAGCCACGTTTTTAACAGAATGAGACACGATTCAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTAACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGC  >  NC_000913/3086477‑3086764                                                                                                                                              |                                                                                                                                                   aGGAAAAATTTCCAGCCACGTTTTTAACAGAATGAGACACGATTCAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCaga                                                                                                                                           <  2:209366/151‑1 (MQ=255)       aaTTTCCAGCCACGTTTTTAACAGAATGAGACACGATTCAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTg                                                                                                                                     >  2:23489/1‑151 (MQ=255)                   cGTTTTTAACAGAATGAGACACGATTCAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCt                                                                                                                         >  1:624268/1‑151 (MQ=255)                        ttAACAGAATGAGACACGATTCAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACcac                                                                                                                    >  1:578713/1‑151 (MQ=255)                          aaCAGAATGAGACACGATTCAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACcaca                                                                                                                   <  2:566430/150‑1 (MQ=255)                             aGAATGAGACACGATTCAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGaa                                                                                                                >  2:541580/1‑150 (MQ=255)                              gAATGAGACACGATTCAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGaaaa                                                                                                              <  2:544876/151‑1 (MQ=255)                               aaTGAGACACGATTCAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAAt                                                                                                             <  1:301735/151‑1 (MQ=255)                                         gATTCAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACacaaca                                                                                                   <  2:464931/151‑1 (MQ=255)                                              aaaaaaaaGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTg                                                                                              <  1:66484/151‑1 (MQ=255)                                               aaaaaaaGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTg                                                                                              >  2:533260/1‑150 (MQ=255)                                                          aaaTAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAAt                                                                                  <  1:494824/151‑1 (MQ=255)                                                                  tGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTATCCAAATTCTCttt                                                                           >  1:430362/1‑150 (MQ=255)                                                                   gaagaaTTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCttt                                                                           >  2:510072/1‑149 (MQ=255)                                                                            aCCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTa                                                                <  2:388978/151‑1 (MQ=255)                                                                             ccTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTa                                                                <  2:162587/150‑1 (MQ=255)                                                                              cTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTaa                                                               >  2:491801/1‑150 (MQ=255)                                                                              cTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTaa                                                               >  1:95612/1‑150 (MQ=255)                                                                              cTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTaa                                                               >  1:65298/1‑150 (MQ=255)                                                                              cTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTaa                                                               >  2:82792/1‑150 (MQ=255)                                                                                aaaaTAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAata                                                            <  1:95797/151‑1 (MQ=255)                                                                                 aaaTAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAatat                                                           <  1:260845/151‑1 (MQ=255)                                                                                            cAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACAcc                                                >  2:483039/1‑151 (MQ=255)                                                                                            cAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACAcc                                                <  1:510083/151‑1 (MQ=255)                                                                                             aGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCt                                               >  2:45920/1‑151 (MQ=255)                                                                                              gATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCtt                                              <  1:38122/151‑1 (MQ=255)                                                                                              gATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCtt                                              <  1:212501/151‑1 (MQ=255)                                                                                                 gTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCttttt                                           >  1:118030/1‑151 (MQ=255)                                                                                                 gTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCtttt                                            <  2:24571/150‑1 (MQ=255)                                                                                                     atccatccATACCGATTAACACTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGt                                       <  1:483049/151‑1 (MQ=255)                                                                                                                        cacTCAGACTGCCAGTGTTTTTGACCTGCAGAGTCGCGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGaa                      >  2:35868/1‑149 (MQ=255)                                                                                                                                  gCCAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGAc          <  1:56928/151‑1 (MQ=255)                                                                                                                                    cAGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACa         >  1:189360/1‑150 (MQ=255)                                                                                                                                     aGTGTTTTTGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACaa        >  2:628886/1‑150 (MQ=255)                                                                                                                                           tttGACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGc  <  1:153132/150‑1 (MQ=255)                                                                                                                                              |                                                                                                                                                   AGGAAAAATTTCCAGCCACGTTTTTAACAGAATGAGACACGATTCAAAAAAAAGTGGAAATAGGGTGAAGAATTGACCTAAAATAGCCATCCAGATGTTAATCCATCCATACCGATTAACACTCAGACTGCCAGTGTTTTTAACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGC  >  NC_000913/3086477‑3086764

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑