Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,089,398	T→C	V372A (GTA→GCA)	galP →	D‑galactose transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,089,398	0	T	C	100.0%	134.5 / NA	35	V372A (GTA→GCA)	galP	D‑galactose transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/17);  total (18/17)

CCTGGTGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGCCACCAACTGGATTGCCAACATGATCGTTGGCGCAACGTTCCTGACCATGCTCAACACGCTGGGTAACGCCAACACCTTCTGGGTGT  >  NC_000913/3089255‑3089544                                                                                                                                                |                                                                                                                                                   ccTGGTGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGCACtgtg                                                                                                                                              <  2:429805/150‑1 (MQ=255)                 ggCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGCACTGTGCTCCGAAATTCAGCCGc                                                                                                                             <  1:196672/151‑1 (MQ=255)                    aTGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGCACTGTGCTCCGAAATTCAGCCGCTg                                                                                                                           <  2:436609/150‑1 (MQ=255)                          gTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGCACTGTGCTCCGAAATTCAGCCGCTGAAAGGcc                                                                                                                    >  2:105690/1‑151 (MQ=255)                                ggTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGCACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGAtt                                                                                                              <  2:144290/151‑1 (MQ=255)                                   aCAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGCACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTg                                                                                                           >  2:585954/1‑151 (MQ=255)                                   aCAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGCACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTg                                                                                                           <  1:263625/151‑1 (MQ=255)                                        gacgCATATCGGTATTCCCTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGCACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCa                                                                                                        <  2:73253/146‑1 (MQ=255)                                                 cGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGCACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTc                                                                                              <  1:529262/150‑1 (MQ=255)                                                           tctcCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGCACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGccacc                                                                                    >  1:580539/1‑150 (MQ=255)                                                              ccGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGCACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGCCACCAACt                                                                                <  1:69125/151‑1 (MQ=255)                                                               cGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGCACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGCCACCAACTg                                                                               >  2:522548/1‑151 (MQ=255)                                                                     cgcAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGCACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGCCACCAACTGGATTGc                                                                         >  1:198270/1‑151 (MQ=255)                                                                       cAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGCACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGCCACCAACTGGATTGCCa                                                                       <  1:462424/151‑1 (MQ=255)                                                                        aGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGCACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGCCACCAACTGGATTGCCa                                                                       >  2:445633/1‑150 (MQ=255)                                                                           aTTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGCACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGCCACCAACTGGATTGCCAACa                                                                    <  1:413340/150‑1 (MQ=255)                                                                                    tcgccatGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGCACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGCCACCAACTGGATTGCCAACATGATCGTTgg                                                          >  1:354269/1‑151 (MQ=255)                                                                                     cgccatGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGCACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGCCACCAACTGGATTGCCAACATGATCGTTgg                                                          <  2:256393/150‑1 (MQ=255)                                                                                              gctgATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGCACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGCCACCAACTGGATTGCCAACATGATCGTTGGCGCAACGTTc                                                >  1:398569/1‑151 (MQ=255)                                                                                                 gATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGCACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGCCACCAACTGGATTGCCAACATGATCGTTGGCGCAACGTTCCTg                                             >  2:622458/1‑151 (MQ=255)                                                                                                  aTGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGCACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGCCACCAACTGGATTGCCAACATGATCGTTGGCGCAACGTTCCTg                                             >  2:567256/1‑150 (MQ=255)                                                                                                  aTGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGCACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGCCACCAACTGGATTGCCAACATGATCGTTGGCGCAACGTTCCTg                                             >  1:217694/1‑150 (MQ=255)                                                                                                         ttGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGCACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGCCACCAACTGGATTGCCAACATGATCGTTGGCGCAACGTTCCTGACCATGc                                      >  2:276267/1‑150 (MQ=255)                                                                                                          tGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGCACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGCCACCAACTGGATTGCCAACATGATCGTTGGCGCAACGTTCCTGACCATGCTc                                    >  1:238843/1‑151 (MQ=255)                                                                                                           gTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGCACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGCCACCAACTGGATTGCCAACATGATCGTTGGCGCAACGTTCCTGACCATGCTc                                    >  2:65341/1‑150 (MQ=255)                                                                                                           gTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGCACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGCCACCAACTGGATTGCAAACATGATCGTTGGCGCAACGTTCCTGACCATGCTCa                                   <  2:435839/151‑1 (MQ=255)                                                                                                                 tttGCCATGAGTGCCGGTCCGCTGATTTGGGCACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGCCACCAACTGGATTGCCAACATGATCGTTGGCGCAACGTTCCTGACCATGCTCAACACGc                             >  1:372593/1‑151 (MQ=255)                                                                                                                  ttGCCATGAGTGCCGGTCCGCTGATTTGGGCACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGCCACCAACTGGATTGCCAACATGATCGTTGGCGCAACGTTCCTGACCATGCTCAACACGc                             <  1:137896/150‑1 (MQ=255)                                                                                                                          aGTGCCGGTCCGCTGATTTGGGCACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGCCACCAACTGGATTGCCAACATGATCGTTGGCGCAACGTTCCTGACCATGCTCAACACGCTGGGTAACg                    <  2:390892/151‑1 (MQ=255)                                                                                                                               cGGTCCGCTGATTTGGGCACTGTGCTCCGAACTTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGCCACCAACTGGATTGCCAACATGATCGTTGGCGCAACGTTCCTGACCATGCTCAACACGCTGGGTAACGCCAac               >  1:238925/1‑151 (MQ=255)                                                                                                                                 gTCCGCTGATTTGGGCACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCGCCTGCTCCACTGCCACCAACTGGATTGCCAACATGATCGTTGGCGCAACGTTCCTGACCATGCTCAACACGCTGGGTAACGCCAacac             <  2:10010/151‑1 (MQ=255)                                                                                                                                     gCTGATTTGGGCACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGCCACCAACTGGATTGCCAACATGATCGTTGGCGCAACGTTCCTGACCATGCTCAACACGCTGGGTAACGCCAACACCTTc         >  2:560998/1‑151 (MQ=255)                                                                                                                                        gATTTGGGCACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGCCACCAACTGGATTGCCAACATGATCGTTGGCGCAACGTTCCTGACCATGCTCAACACGCTGGGTAACGCCAACACCTTCt        <  1:218729/149‑1 (MQ=255)                                                                                                                                            tGGGCACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGCCACCAACTGGATTGCCAACATGATCGTTGGCGCAACGTTCCTGACCATGCTCAACACGCTGGGTAACGCCAACACCTTCTGGgtgt  >  2:412411/1‑151 (MQ=255)                                                                                                                                            tGGGCACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGCCACCAACTGGATTGCCAACATGATCGTTGGCGCAACGTTCCTGACCATGCTCAACACGCTGGGTAACGCCAACACCTTCTGGgtg   <  1:257850/150‑1 (MQ=255)                                                                                                                                                |                                                                                                                                                   CCTGGTGATGGCTGCTGGCATGGGCGTACTCGGTACAATGATGCATATCGGTATTCACTCTCCGTCGGCGCAGTATTTCGCCATCGCCATGCTGCTGATGTTTATTGTCGGTTTTGCCATGAGTGCCGGTCCGCTGATTTGGGTACTGTGCTCCGAAATTCAGCCGCTGAAAGGCCGCGATTTTGGCATCACCTGCTCCACTGCCACCAACTGGATTGCCAACATGATCGTTGGCGCAACGTTCCTGACCATGCTCAACACGCTGGGTAACGCCAACACCTTCTGGGTGT  >  NC_000913/3089255‑3089544

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑