Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,106,035	C→T	P108S (CCA→TCA)	nupG →	nucleoside transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,106,035	0	C	T	100.0%	158.3 / NA	43	P108S (CCA→TCA)	nupG	nucleoside transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/26);  total (17/26)

GGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGCCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCGCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCAATGTGGGTGGTGAGCCTGTCTGGCTTCG  >  NC_000913/3105887‑3106179                                                                                                                                                     |                                                                                                                                                 ggggATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGTCa                                                                                                                                                <  1:312922/151‑1 (MQ=255)  gggATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTCATTAACTCGTTTGCTTATATGTCaa                                                                                                                                               <  1:164798/151‑1 (MQ=255)     aTTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGTCAACGc                                                                                                                                            >  2:534086/1‑151 (MQ=255)        gtgGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGTCAACGCTTg                                                                                                                                         <  1:292046/151‑1 (MQ=255)        gtgGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCAAAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGTCAACGCTTg                                                                                                                                         >  2:414457/1‑151 (MQ=255)         tgGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGTCAACGCTTgg                                                                                                                                        >  1:167647/1‑151 (MQ=255)            ccGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGTCAACGCTTGGGtt                                                                                                                                     <  2:514924/151‑1 (MQ=255)                cAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACTCCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGTCAACGCTTGGGTTaac                                                                                                                                  <  2:613795/150‑2 (MQ=255)                cAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGTCAACGCTTGGGTTAATc                                                                                                                                 >  1:546952/1‑151 (MQ=255)                  aaTGGTTAAGCGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGTCAACGCTTGGGTTAATCa                                                                                                                                >  1:498022/1‑150 (MQ=255)                         aaGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGTCAACGCTTGGGTTAATCAACACCAt                                                                                                                         <  2:199/150‑1 (MQ=255)                         aaGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGTCAACGCTTGGGTTAATCAACACCAt                                                                                                                         <  1:402872/150‑1 (MQ=255)                          aGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGTCAACGCTTGGGTTAATCAACACCAtct                                                                                                                       <  2:427283/151‑1 (MQ=255)                                aaaTGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGTCAACGCTTGGGTTAATCAACACCATCTCTTACt                                                                                                                 >  1:571910/1‑151 (MQ=255)                                 aaTGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGTCAACGCTTGGGTTAATCAACACCATCTCTTACTa                                                                                                                <  2:222532/151‑1 (MQ=255)                                  aTGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGTCAACGCTTGGGTTAATCAACACCATCTCTTACTAt                                                                                                               >  2:188563/1‑151 (MQ=255)                                  aTGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGTCAACGCTTGGGTTAATCAACACCATCTCTTACTAt                                                                                                               <  1:287654/151‑1 (MQ=255)                                    gggTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGTCAACGCTTGGGTTAATCAACACCATCTCTTACTATc                                                                                                              <  2:288331/150‑1 (MQ=255)                                    gggTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGTCAACGCTTGGGTTAATCAACACCATCTCTTACTATc                                                                                                              <  2:625359/150‑1 (MQ=255)                                        atatGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGTCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCt                                                                                                          <  2:322339/150‑1 (MQ=255)                                        atatGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGTCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTg                                                                                                         >  1:6681/1‑151 (MQ=255)                                            gCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGTCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCaa                                                                                                      >  2:38944/1‑150 (MQ=255)                                              cATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGTCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAAt                                                                                                   <  1:224822/151‑1 (MQ=255)                                              cATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGTCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAAt                                                                                                   >  1:553572/1‑151 (MQ=255)                                                      acacCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGTCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGggat                                                                                           <  1:444247/151‑1 (MQ=255)                                                        acCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGTCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGggatgg                                                                                         <  2:300098/151‑1 (MQ=255)                                                              ggCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGTCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATc                                                                                    <  1:458947/150‑1 (MQ=255)                                                               gcgcTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGTCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGata                                                                                      <  1:86673/147‑1 (MQ=255)                                                                cgcTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGTCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGtt                                                                                 >  2:361933/1‑151 (MQ=255)                                                                 gcTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGTCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTa                                                                                <  1:621917/151‑1 (MQ=255)                                                                 gcTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGTCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTa                                                                                <  1:348615/151‑1 (MQ=255)                                                                                aTGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGTCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCGCCaa                                                                 <  1:405046/151‑1 (MQ=255)                                                                                                    acCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGTCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCGCCAATCCGTATCTGGGGCACCATc                                             >  2:211275/1‑151 (MQ=255)                                                                                                           gCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGTCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCGCCAATCCGTATCTGGGGCACCATCGGCttt                                       >  2:212823/1‑150 (MQ=255)                                                                                                                 ttCCTTGTGATATTGATTAACTCGTTTGCTTATATGTCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCGCCAATCCGTATCTGGGGCACCATCGGCTTTATCATg                                 <  1:155279/150‑1 (MQ=255)                                                                                                                   ccTTGTGATATTGATTAACTCGTTTGCTTATATGTCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCGCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCa                              <  2:222866/151‑1 (MQ=255)                                                                                                                        tgATATTGATTAACTCGTTTGCTTATATGTCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCGCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCAAt                            <  1:123862/148‑1 (MQ=255)                                                                                                                            atTGATTAACTCGTTTGCTTATATGTCAACGCTTGGGTTAATCAACCCCATCTCTTACTATCTCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCGCCAATCCGTATCTGGGGCACCAGCGGCTTTATCATGGCAATgtgggtg                     <  1:521273/151‑1 (MQ=255)                                                                                                                            atTGATTAACTCGTTTGCTTATATGTCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCGCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCAATgtgggt                      >  1:368374/1‑150 (MQ=255)                                                                                                                              tGATTAACTCGTTTGCTTATATGTCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCGCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCAATGTGggtggt                   >  2:610950/1‑151 (MQ=255)                                                                                                                                       cGTTTGCTTATATGTCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCGCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCAATGTGGGTGGTGAGCCTg            <  2:236201/149‑1 (MQ=255)                                                                                                                                             cTTATATGTCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCGCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCAATGTGGGTGGTGAGCCTGTCTGGCtt    >  2:523755/1‑151 (MQ=255)                                                                                                                                               tataTGTCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCGCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCAATGTGGGTGGTGAGCCTGTCTGGCTTCg  >  1:328490/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                                 GGGGATTGTGGCCGACAAATGGTTAAGTGCGAAATGGGTATATGCCATTTGCCACACCATTGGCGCTATCACGCTGTTCATGGCGGCACAGGTCACGACACCGGAAGCGATGTTCCTTGTGATATTGATTAACTCGTTTGCTTATATGCCAACGCTTGGGTTAATCAACACCATCTCTTACTATCGCCTGCAAAATGCCGGGATGGATATCGTTACTGACTTCCCGCCAATCCGTATCTGGGGCACCATCGGCTTTATCATGGCAATGTGGGTGGTGAGCCTGTCTGGCTTCG  >  NC_000913/3105887‑3106179

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑