Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,297,933	T→C	Y69C (TAC→TGC)	yraQ ←	putative inner membrane permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,297,933	0	T	C	100.0%	78.6 / NA	23	Y69C (TAC→TGC)	yraQ	putative inner membrane permease
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/11);  total (12/11)

AGCGTTCCCAGCAGCGTGCCGCGAAAGCGCGATTGCCCAAGCGTACGCAACAACCAGTCACGCGGGATCAGCACCTGAATCAACGAACCGAGGATCACCCCCAGCACCGCCGCTTTCCATACCGCGAGGAAATAGATCATCGCGTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAA  >  NC_000913/3297789‑3298078                                                                                                                                                 |                                                                                                                                                  aGCGTTCCCAGCAGCGTGCCGCGAAAGCGCGATTGCCCAAGCGTACGCAACACCCAGTCACGCGGGATCAGCACCTGAATCAACGAACCGAGGATCACCCCCAGCACCGCCGCTTTCCATACCGCGAGGAAATAGATCATCGCGCAATCCa                                                                                                                                             <  1:177016/151‑1 (MQ=255)         cagcagCGTGCCGCGAAAGCGCGATTGCCCAAGCGTACGCAACAACCAGTCACGCGGGATCAGCACCTGAATCAACGAACCGAGGATCACCCCCAGCACCGCCGCTTTCCATACCGCGAGGAAATAGATCATCGCGCAATCCAACgccgcc                                                                                                                                     <  1:352214/151‑1 (MQ=255)                       gAAAGCGCGATTGCCCAAGCGTACGCAACAACCAGTCACGCGGGATCAGCACCTGAATCAACGAACCGAGGATCACCCCCAGCACCGCCGCTTTCCATACCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTa                                                                                                                         <  2:81307/149‑1 (MQ=255)                            cgcgATTGCCCAAGCGTACGCAACAACCAGTCACGCGGGATCAGCACCTGAATCAACGAACCGAGGATCACCCCCAGCACCGCCGCTTTCCATACCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCg                                                                                                                  >  2:497546/1‑151 (MQ=255)                             gcgATTGCCCAAGCGTACGCAACAACCAGTCACGCGGGATCAGCACCTGAATCAACGAACCGAGGATCACCCCCAGCACCGCCGCTTTCCATACCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGc                                                                                                                 >  1:203466/1‑151 (MQ=255)                                              cGCAACAACCAGTCACGCGGGATCAGCACCTGAATCAACGAACCGAGGATCACCCCCAGCACCGCCGCTTTCCATACCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGAtt                                                                                                >  1:552384/1‑151 (MQ=255)                                                   caacCAGTCACGCGGGATCAGCACCTGAATCAACGAACCGAGGATCACCCCCAGCACCGCCGCTTTCCATACCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCg                                                                                           >  2:529854/1‑151 (MQ=255)                                                               cgGGATCAGCACCTGAATCAACGAACCGAGGATCACCCCCAGCACCGCCGCTTTCCATACCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGtt                                                                               >  1:191440/1‑151 (MQ=255)                                                                gggATCAGCACCTGAATCAACGAACCGAGGATCCCCCCCAGCACCGCCGCTTTCCATACCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGttt                                                                              <  1:353463/151‑1 (MQ=255)                                                                 ggATCAGCACCTGAATCAACGAACCGAGGATCACCCCCAGCACCGCCGCTTTCCATACCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTc                                                                             <  1:130032/151‑1 (MQ=255)                                                                  gATCAGCACCTGAATCAACGAACCGAGGATCACCCCCAGCACCGCCGCTTTCCATACCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCg                                                                            >  1:69705/1‑151 (MQ=255)                                                                        ccccaacaTCACCGAACCGAGGATCCCCCCCAGAACCGCCGCTTTCCATACCGCGAGGAAATAGCTCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCg                                                                            <  2:62097/138‑1 (MQ=255)                                                                              aaTCAACGAACCGAGGATCACCCCCAGCACCGCCGCTTTCCATACCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAAc                                                                >  2:514710/1‑151 (MQ=255)                                                                                      aaCCGAGGATCACCCCCAGCACCGCCGCTTTCCATACCGCGACGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGcc                                                        >  1:65386/1‑151 (MQ=255)                                                                                       aCCGAGGATCACCCCCAGCACCGCCGCTTTCCATACCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCg                                                       >  1:36603/1‑151 (MQ=255)                                                                                                              ccgcTTTCCATACCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATa                                <  1:294289/151‑1 (MQ=255)                                                                                                              ccgcTTTCCATACCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATa                                <  2:517792/151‑1 (MQ=255)                                                                                                                   ttCCATACCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCag                            <  2:412447/150‑1 (MQ=255)                                                                                                                   ttCCATACCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCa                             <  1:323117/149‑1 (MQ=255)                                                                                                                         aCCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCgg                     >  1:360108/1‑151 (MQ=255)                                                                                                                         aCCGCGAGGAAATAGATCATCGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCgg                     >  1:176940/1‑151 (MQ=255)                                                                                                                                           atcGCGCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGagaa      >  1:88641/1‑148 (MQ=255)                                                                                                                                             cgcgCAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGagaaagaa  <  1:560702/150‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                  AGCGTTCCCAGCAGCGTGCCGCGAAAGCGCGATTGCCCAAGCGTACGCAACAACCAGTCACGCGGGATCAGCACCTGAATCAACGAACCGAGGATCACCCCCAGCACCGCCGCTTTCCATACCGCGAGGAAATAGATCATCGCGTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAA  >  NC_000913/3297789‑3298078

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑