Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,314,066	G→A	A650V (GCG→GTG)	infB ←	translation initiation factor IF‑2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,314,066	0	G	A	100.0%	115.7 / NA	31	A650V (GCG→GTG)	infB	translation initiation factor IF‑2
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/17);  total (14/17)

TGTACGTCTGCCTTCAGGACGATATTCACTTCGTGAACTTCGCCTTCGGTCATGTTGGCGAACATGTTCTCGAGTTTAGATTTCTGCTGACGCGCCAGTTTAACTTCGCGGAATTTACCCTGACGATAGAGTGCAACTTCACGCGCTTTCTTCTCGTCACGTACAACGGTAACTTCATCACCCGCAGCCGGTACGCCGGACAGGCCGAGGATTTCCACCGGAATGGACGGACCCGCTTCCAGCACTTCCTGACCCAGTTCGTTACGCATCGCACGAACACGACCGT  >  NC_000913/3313922‑3314207                                                                                                                                                 |                                                                                                                                              tGTACGTCTGCCTTCAGGACGATATTCACTTCGTGAACTTCGCCTTCGGTCATGTTGGCGAACATGTTCTCGAGTTTAGATTTCTGCTGACGCGCCAGTTTAACTTCGCGGAATTTACCCTGACGATAGAGTGCAACTTCACGCACTttct                                                                                                                                         >  1:510928/1‑151 (MQ=255)  gTACGTCTGCCTTCAGGACGATATTCACTTCGTGAACTTCGCCTTCGGTCATGTTGGCGAACATGTTCTCGAGTTTAGATTTCTGCTGACGCGCCAGTTTAACTTCGCGGAATTTACCCTGACGATAGAGTGCAACTTCACGCACTttct                                                                                                                                         <  1:334410/150‑1 (MQ=255)      gTCTGCCTTCAGGACGATATTCACTTCGTGAACTTCGCCTTCGGTCATGTTGGCGAACATGTTCTCGAGTTTAGATTTCTGCTGACGCGCCAGTTTAACTTCGCGGAATTTATCCTGACGATAGAGTGCAACTTCACGCACTTTCTTCTCg                                                                                                                                    <  1:404287/151‑1 (MQ=255)                   aCGATATTCACTTCGTGAACTTCGCCTTCGGTCATGTTGGCGAACATGTTCTCGAGTTTAGATTTCTGCTGACGCGCCAGTTTAACTTCGCGGAATTTACCCTGACGATAGAGTGCAACTTCACGCACTTTCTTCTCGTCACGTACAACgg                                                                                                                       >  2:211416/1‑151 (MQ=255)                      atatTCACTTCGTGAACTTCGCCTTCGGTCATGTTGGCGAACATGTTCTCGAGTTTAGATTTCTGCTGACGCGCCAGTTTAACTTCGCGGAATTTACCCTGACGATAGAGTGCAACTTCACGCACTTTCTTCTCGTCACGTACAACGGTaa                                                                                                                    <  1:122176/151‑1 (MQ=255)                       tatTCACTTCGTGAACTTCGCCTTCGGTCATGTTGGCGAACATGTTCTCGAGTTTAGATTTCTGCTGACGCGCCAGTTTAACTTCGCGGAATTTACCCTGACGATAGAGTGCAACTTCACGCACTTTCTTCTCGTCACGTACAACGGTaa                                                                                                                    >  2:122607/1‑150 (MQ=255)                               tCGTGAACTTCGCCTTCGGTCATGTTGGCGAACATGTTCTCGAGTTTAGATTTCTGCTGACGCGCCAGTTTAACTTCGCGGAATTTACCCTGACGATAGAGTGCAACTTCACGCACTTTCTTCTCGTCACGTACAACGGTAACTtcatc                                                                                                             <  1:98382/149‑1 (MQ=255)                                 gTGAACTTCGCCTTCGGTCATGTTGGCGAACATGTTCTCGAGTTTAGATTTCTGCTGACGCGCCAGTTTAACTTCGCGGAATTTACCCTGACGATAGAGTGCAACTTCACGCACTTTCTTCTCGTCACGTACAACGGTAACTTCATCAccc                                                                                                         <  2:570315/151‑1 (MQ=255)                                          gCCTTCGGTCATGTTGGCGAACATGTTCTCGAGTTTAGATTTCTGCTGACGCGCCAGTTTAACTTCGCGGAATTTACCCTGACGATAGAGTGCAACTTCACGCACTTTCTTCTCGTCACGTACAACGGTAACTTCATCACCCGCAGCCGGt                                                                                                <  1:469038/151‑1 (MQ=255)                                              tCGGTCATGTTGGCGAACATGTTCTCGAGTTTAGATTTCTGCTGACGCGCCAGTTTAACTTCGCGGAATTTACCCTGACGATAGAGTGCAACTTCACGCACTTTCTTCTCGTCACGTACAACGGTAACTTCATCACCCGCAGCCGGTACg                                                                                             <  2:25764/150‑1 (MQ=255)                                                 gTCATGTTGGCGAACATGTTCTCGAGTTTAGATTTCTGCTGACGCGCCAGTTTAACTTCGCGGAATTTACCCTGACGATAGAGTGCAACTTCACGCACTTTCTTCTCGTCACGTACAACGGTAACTTCATCACCCGCAGCCGGTACGCCgg                                                                                         <  2:616527/151‑1 (MQ=255)                                                  tCATGTTGGCGAACATGTTCTCGAGTTTAGATTTCTGCTGACGCGCCAGTTTAACTTCGCGGAATTTACCCTGACGATAGAGTGCAACTTCACGCACTTTCTTCTCGTCACGTACAACGGTAACTTCATCACCCGCAGCCGGTACGCCgg                                                                                         >  2:360523/1‑150 (MQ=255)                                                      gTTGGCGAACATGTTCTCGAGTTTAGATTTCTGCTGACGCGCCAGTTTAACTTCGCGGAATTTACCCTGACGATAGAGTGCAACTTCACGCACTTTCTTCTCGTCACGTACAACGGTAACTTCATCACCCGCAGCCGGTACGCCGGACAgg                                                                                    <  2:537793/151‑1 (MQ=255)                                                        tGGCGAACATGTTCTCGAGTTTAGATTTCTGCTGACGCGCCAGTTTAACTTCGCGGAATTTACCCTGACGATAGAGTGCAACTTCACGCACTTTCTTCTCGTCACGTACAACGGAAACTTCATCACCCGCAGCCGGTACGCCGGACAGGcc                                                                                  >  2:20077/1‑151 (MQ=255)                                                                 tGTTCTCGAGTTTAGATTTCTGCTGACGCGCCAGTTTAACTTCGCGGAATTTACCCTGACGATAGAGTGCAACTTCACGCACTTTCTTCTCGTCACGTACAACGGTAACTTCATCACCCGCAGCCGGTACGCCGGACAGGCCGAGGATTTc                                                                         >  1:544645/1‑151 (MQ=255)                                                                           tttAGATTTCTGCTGACGCGCCAGTTTAACTTCGCGGAATTTACCCTGACGATAGAGTGCAACTTCACGCACTTTCTTCTCGTCACGTACAACGGTAACTTCATCACCCGCAGCCGGTACGCCGGACAGGCCGAGGATTTCCACCGGAATg                                                               >  2:202315/1‑151 (MQ=255)                                                                               gATTTCTGCTGACGCGCCAGTTTAACTTCGCGGAATTTACCCTGACGATAGAGTGCAACTTCACGCACTTTCTTCTCGTCACGTACAACGGTAACTTCATCACCCGCAGCCGGTACGCCGGACAGGCCGAGGATTTCCACCGGAATggacg                                                           >  1:457804/1‑151 (MQ=255)                                                                                   tCTGCTGACGCGCCAGTTTAACTTCGCGGAATTTACCCTGACGATAGAGTGCAACTTCACGCACTTTCTTCTCGTCACGTACAACGGTAACTTCATCACCCGCAGCCGGTACGCCGGACAGGCCGAGGATTTCCACCGGAATGGACGGAcc                                                       >  2:490674/1‑151 (MQ=255)                                                                                        tgACGCGCCAGTTTAACTTCGCGGAATTTACCCTGACGATAGAGTGCAACTTCACGCACTTTCTTCTCGTCACGTACAACGGTAACTTCATCACCCGCAGCCGGTACGCCGGACAGGCCGAGGATTTCCACCGGAATGGACGGACCCGCtt                                                  >  2:438225/1‑151 (MQ=255)                                                                                         gACGCGCCAGTTTAACTTCGCGGAATTTACCCTGACGATAGAGTGCAACTTCACGCACTTTCTTCTCGTCACGTACAACGGTAACTTCATCACCCGCAGCCGGTACGCCGGACAGGCCGAGGATTTCCACCGGAATGGACGGACCCGCt                                                   <  1:430024/149‑1 (MQ=255)                                                                                          aCGCGCCAGTTTAACTTCGCGGAATTTACCCTGACGATAGAGTGCAACTTCACGCACTTTCTTCTCGTCACGTACAACGGTAACTTCATCACCCGCAGCCGGTACGCCGGACAGGCCGAGGATTTCCACCGGAATGGACGGACCCGCTTcc                                                >  2:37210/1‑151 (MQ=255)                                                                                              gcCAGTTTAACTTCGCGGAATTTACCCTGACGATAGAGTGCAACTTCACGCACTTTCTTCTCGTCACGTACAACGGTAACTTCATCACCCGCAGCCGGTACGCCGGACAGGCCGAGGATTTCCACCGGAATGGACGGACCCGCTTCCAGCa                                            <  2:371928/151‑1 (MQ=255)                                                                                               ccAGTTTAACTTCGCGGAATTTACCCTGACGATAGAGTGCAACTTCACGCACTTTCTTCTCGTCACGTACAACGGTAACTTCATCGCCCGCAGCCGGTACGCCGGACAGGCCGAGGATTTCCACCGGAATGGACGGACCCGCTTCCAGCAc                                           <  1:533669/151‑1 (MQ=255)                                                                                                  gTTTAACTTCGCGGAATTTACCCTGACGATAGAGTGCAACTTCACGCACTTTCTTCTCGTCACGTACAACGGTAACTTCATCACCCGCAGCCGGTACGCCGGACAGGCCGAGGATTTCCACCGGAATGGACGGACCCGCTTCCAGCACTTc                                        >  1:625288/1‑151 (MQ=255)                                                                                                       aCTTCGCGGAATTTACCCTGACGATAGAGTGCAACTTCACGCACTTTCTTCTCGTCACGTACAACGGTAACTTCATCACCCGCAGCCGGTACGCCGGACAGGCCGAGGATTTCCACCGGAATGGACGGACCCGCTTCCAGCACTTCCTGAc                                   <  2:166658/151‑1 (MQ=255)                                                                                                          tCGCGGAATTTACCCTGACGATAGAGTGCAACTTCACGCACTTTCTTCTCGTCACGTACAACGGTAACTTCATCACCCGCAGCCGGTACGCCGGACAGGCCGAGGATTTCCACCGGAATGGACGGACCCGCTTCCAGCACTTCCTGAccc                                 <  1:56352/150‑1 (MQ=255)                                                                                                           cgcgGAATTTACCCTGACGATAGAGTGCAACTTCACGCACTTTCTTCTCGTCACGTACAACGGTAACTTCATCACCCGCAGCCGGTACGCCGGACAGGCCGAGGATTTCCACCGGAATGGACGGACCCGCTTCCAGCACTTCCTGAccc                                 >  1:458457/1‑149 (MQ=255)                                                                                                              ggAATTTACCCTGACGATAGAGTGCAACTTCACGCACTTTCTTCTCGTCACGTACAACGGTAACTTCATCACCCGCAGCCGGTACGCCGGACAGGCCGAGGATTTCCACCGGAATGGACGGACCCGCTTCCAGCACTTCCTGACCCAGTTc                            <  2:230536/151‑1 (MQ=255)                                                                                                                   ttACCCTGACGATAGAGTGCAACTTCACGCACTTTCTTCTCGTCACGTACAACGGTAACTTCATCACCCGCAGCCGGTACGCCGGACAGGCCGAGGATTTCCACCGGAATGGACGGACCCGCTTCCAGCACTTCCTGACCCAGTTCGTTa                        <  1:166752/150‑1 (MQ=255)                                                                                                                                  agTGCAACTTCACGCACTTTCTTCTCGTCACGTACAACGGTAACTTCATCACCCGCAGCCGGTACGCCGGACAGGCCGAGGATTTCCACCGGAATGGACGGACCCGCTTCCAGCACTTCCTGACCCAGTTCGTTACGCATCGCACGAacac        <  2:457465/151‑1 (MQ=255)                                                                                                                                         cTTCACGCACTTTCTTCTCGTCACGTACAACGGTAACTTCATCACCCGCAGCCGGTACGCCGGACAGGCCGAGGATTTCCACCGGAATGGACGGACCCGCTTCCAGCACTTCCTGACCCAGTTCGTTACGCATCGCACGAACACGACCGt  >  1:277394/1‑150 (MQ=255)                                                                                                                                                 |                                                                                                                                              TGTACGTCTGCCTTCAGGACGATATTCACTTCGTGAACTTCGCCTTCGGTCATGTTGGCGAACATGTTCTCGAGTTTAGATTTCTGCTGACGCGCCAGTTTAACTTCGCGGAATTTACCCTGACGATAGAGTGCAACTTCACGCGCTTTCTTCTCGTCACGTACAACGGTAACTTCATCACCCGCAGCCGGTACGCCGGACAGGCCGAGGATTTCCACCGGAATGGACGGACCCGCTTCCAGCACTTCCTGACCCAGTTCGTTACGCATCGCACGAACACGACCGT  >  NC_000913/3313922‑3314207

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑