Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,319,794	T→C	R386R (CGT→CGC)	argG →	argininosuccinate synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,319,794	0	T	C	100.0%	114.8 / NA	32	R386R (CGT→CGC)	argG	argininosuccinate synthetase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/16);  total (16/16)

TGACTCCCAGGCGCTGATGCTGCGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCACTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGTCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACTGATACCCGCGAGAAACTTTTCGGTTATGCCAAAACTGGCCTGCTTTCCTCCTCTGCCGCTTCAGG  >  NC_000913/3319647‑3319940                                                                                                                                                    |                                                                                                                                                   tGACTCCCAGGCGCTGATGCTGCGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCACTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTg                                                                                                                                                 >  2:249623/1‑151 (MQ=255)          ggCGCTGATGCTGCGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCACTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGa                                                                                                                                         >  2:255423/1‑150 (MQ=255)            cgcTGATGCTGCGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCACTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGaaaa                                                                                                                                      <  2:516065/151‑1 (MQ=255)              cTGATGCTGCGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCACTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGaaaa                                                                                                                                      >  2:610512/1‑149 (MQ=255)                 aTGCTGCGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCACTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCg                                                                                                                                 <  2:393048/151‑1 (MQ=255)                      gcGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCACTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACt                                                                                                                              <  1:255430/149‑1 (MQ=255)                           aCTCTCTGCAACGCTGGGTTGCCAGCCAGATCACTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGGAAAAGGCGACTCGGTGtt                                                                                                                       >  1:602310/1‑151 (MQ=255)                             tctctGCAACGCTGGGTTGCCAGCCAGATCACTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTtct                                                                                                                     >  2:57431/1‑151 (MQ=255)                               tctGCAACGCTGGGTTGCCAGCCAGATCACTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCg                                                                                                                   >  2:41733/1‑151 (MQ=255)                                ctGCAACGCTGGGTTGCCAGCCAGATCACTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTtctc                                                                                                                    <  2:24719/149‑1 (MQ=255)                                ctGCAACGCTGGGTTGCCAGCCAGATCACTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTtct                                                                                                                     <  1:607477/148‑1 (MQ=255)                                ctGCAACGCTGGGTTGCCAGCCAGATCACTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTtct                                                                                                                     <  2:313989/148‑1 (MQ=255)                                   cAACGCTGGGTTGCCAGCCAGATCACTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGcc                                                                                                                 >  2:557004/1‑149 (MQ=255)                                                           aCTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCa                                                                                       <  1:596764/151‑1 (MQ=255)                                                              ggTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGc                                                                                    <  2:285969/151‑1 (MQ=255)                                                                  aaGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTa                                                                                 >  1:77148/1‑150 (MQ=255)                                                                       aCCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTgg                                                                           <  2:105401/151‑1 (MQ=255)                                                                              aGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCAc                                                                    >  2:315691/1‑151 (MQ=255)                                                                                   cgcCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACTGATa                                                               <  2:297485/151‑1 (MQ=255)                                                                                      cGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACTGATAccc                                                            <  2:631803/151‑1 (MQ=255)                                                                                            aaCGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACTGATACCcgcg                                                         >  1:370850/1‑148 (MQ=255)                                                                                                  tattCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACTGATACCCGCGAGAAACttt                                                >  2:583671/1‑151 (MQ=255)                                                                                                      cAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACTGATACCCGCGAGAAACTTTTCgg                                            <  2:485181/151‑1 (MQ=255)                                                                                                        aTCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACTGATACCCGCGAGAAACTTTTCGGtt                                          >  2:147721/1‑151 (MQ=255)                                                                                                          ccTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACTGATACCCGCGAGAAACTTTTCGGTTa                                         <  2:368819/150‑1 (MQ=255)                                                                                                                 aCCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACTGATACCCGCGAGAAACTTTTCGGTTATGCCaaa                                  <  2:141000/150‑1 (MQ=255)                                                                                                                       tcAGAGAACCTGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACTGATACCCGCGAGAAACTTTTCGGTTATGCCAAAACTGGcc                           >  1:523694/1‑151 (MQ=255)                                                                                                                                 tGACCTACAAGCCAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACTGATACCCGCGAGAAACTTTTCGGTTATGCCAAAACTGGCCTGCTTtcctc                 >  1:139991/1‑151 (MQ=255)                                                                                                                                             cAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACTGATACCCGCGAGAAACTTTTCGGTTATGCCAAAACTGGCCTGCTTTCCTCCTCTGCCGCTTc     >  1:512892/1‑151 (MQ=255)                                                                                                                                             cAGAGCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACTGATACCCGCGAGAAACTTTTCGGTTATGCCAAAACTGGCCTGCTTTCCTCCTCTGCCGCTTc     >  2:419361/1‑151 (MQ=255)                                                                                                                                              agagCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACTGATACCCGCGAGAAACTTTTCGGTTATGCCAAAACTGGCCTGCTTTCCTCCTCTGCCGCTTCa    <  2:185912/151‑1 (MQ=255)                                                                                                                                                agCGCCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACTGATACCCGCGAGAAACTTTTCGGTTATGCCAAAACTGGCCTGCTTTCCTCCTCTGCCGCTTCAgg  <  2:224939/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                   TGACTCCCAGGCGCTGATGCTGCGTGACTCTCTGCAACGCTGGGTTGCCAGCCAGATCACTGGTGAAGTTACCCTGGAGCTGCGCCGTGGGAACGATTATTCAATCCTGAATACCGTCTCAGAGAACCTGACCTACAAGCCAGAGCGTCTGACGATGGAAAAAGGCGACTCGGTGTTCTCGCCAGATGATCGTATTGGTCAATTGACCATGCGTAACCTGGATATCACTGATACCCGCGAGAAACTTTTCGGTTATGCCAAAACTGGCCTGCTTTCCTCCTCTGCCGCTTCAGG  >  NC_000913/3319647‑3319940

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑