Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,376,403	T→C	D125G (GAC→GGC)	sspB ←	ClpXP protease specificity enhancing factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,376,403	0	T	C	100.0%	115.1 / NA	33	D125G (GAC→GGC)	sspB	ClpXP protease specificity enhancing factor
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/15);  total (18/15)

CAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGTCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTCATCGTAGGCAGCTTCAGGCTCAAACATCGTGCCTGCGCCATTTTCACGGGCGTAGATAGCCAGCACGGCAGCCAGCGGCACAGAAACCTGACGCGGAATGCCACC  >  NC_000913/3376255‑3376548                                                                                                                                                     |                                                                                                                                                  cAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGCCt                                                                                                                                                 >  2:582096/1‑151 (MQ=255)      cgccTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGCCTGCCGa                                                                                                                                            <  1:105887/151‑1 (MQ=255)            ggCCTGTTTTGTATTACTTCACACCGCGTAAGGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTAATCGTCAGGATGGGTGTCATCATCGTGACCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGCCTGCCGATGCctc                                                                                                                                      <  2:627623/151‑1 (MQ=255)             gCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGCCTGCCGATGCctct                                                                                                                                     >  1:164210/1‑151 (MQ=255)                       tATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGCCTGCCGATGCCTCTTCAtcattc                                                                                                                            <  2:515000/150‑1 (MQ=255)                           aCTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGCCTGCCGATGCCTCTTCATCATTCatgat                                                                                                                       >  2:396001/1‑151 (MQ=255)                                    cgcgTAATGCCGGTCGACCGCCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGCCTGCCGATGCCTCTTCATCATTCATGATGCTGGTAt                                                                                                               <  2:7291/150‑1 (MQ=255)                                     gcgTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGCCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCt                                                                                                             >  1:489386/1‑151 (MQ=255)                                      cgTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGCCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCtt                                                                                                            <  1:143725/151‑1 (MQ=255)                                          aTGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGCCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTCATc                                                                                                        <  1:406578/151‑1 (MQ=255)                                              cGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGCCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTCATCGTAg                                                                                                    <  1:609982/151‑1 (MQ=255)                                                       accGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGCCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTCATCGTAGGCAGCTTc                                                                                            >  1:610592/1‑150 (MQ=255)                                                            gtggtgGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGCCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTCATCGTAGGCAGCTTCAGGCTc                                                                                      <  2:26370/151‑1 (MQ=255)                                                               gtggCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGCCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTCATCGTAGGCAGCTTCAGGCTCaa                                                                                    >  1:147844/1‑150 (MQ=255)                                                                                    cAGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGCCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTCATCGTAGGCAGCTTCAGGCTCAAACATCGTGCCTGCGCCATTTTc                                                              >  2:105343/1‑151 (MQ=255)                                                                                        aTGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGCCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTCATCGTAGGCAGCTTCAGGCTCAAACATCGTGCCTGCGCCATTTTCACgg                                                          >  2:341663/1‑151 (MQ=255)                                                                                          gAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGCCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTCATCGTAGGCAGCTTCAGGCTCAAACATCGTGCCTGCGCCATTTTCACGGGc                                                        <  2:507662/151‑1 (MQ=255)                                                                                                catcatCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGCCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTCATCGTAGGCAGCTTCAGGCTCAAACATCGTGCCTGCGCCATTTTCACGGGCGTAGa                                                   <  1:556163/150‑1 (MQ=255)                                                                                                catcatCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGCCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTCATCGTAGGCAGCTTCAGGCTCAAACATCGTGCCTGCGCCATTTTCACGGGCGTAGa                                                   <  1:582111/150‑1 (MQ=255)                                                                                                     tcGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGCCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTCATCGTAGGCAGCTTCAGGCTCAAACATCGTGCCTGCGCCATTTTCACGGGCGTAGATAGcc                                              >  1:234584/1‑150 (MQ=255)                                                                                                                cTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGCCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTCATCGTAGGCAGCTTCAGGCTCAAACATCGTGCCTGCGCCATTTTCACGGGCGTAGATAGCCAGCACGGcagcc                                  >  2:219627/1‑151 (MQ=255)                                                                                                                        cATCAATAACCGACATAACGGTTTCGTTGCCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTCATCGTAGGCAGCTTCAGGCTCAAACATCGTGCCTGCGCCATTTTCACGGGCGTAGATAGCCAGCACGGCAGCCAGCGGcac                          <  2:325861/151‑1 (MQ=255)                                                                                                                            aaTAACCGACATAACGGTTTCGTTGCCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTCATCGTAGGCAGCTTCAGGCTCAAACATCGTGCCTGCGCCATTTTCACGGGCGTAGATAGCCAGCACGGCAGCCAGCGGCACAGaa                      <  2:553473/151‑1 (MQ=255)                                                                                                                            aaTAACCGACATAACGGTTTCGTTGCCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTCATCGTAGGCAGCTTCAGGCTCAAACATCGTGCCTGCGCCATTTTCACGGGCGTAGATAGCCAGCACGGCAGCCAGCGGCACAGa                       >  1:400264/1‑150 (MQ=255)                                                                                                                             aTAACCGACATAACGGTTTCGTTGCCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTCATCGTAGGCAGCTTCAGGCTCAAACATCGTGCCTGCGCCATTTTCACGGGCGTAGATAGCCAGCACGGCAGCCAGCGGCACAGaaa                     <  1:428347/151‑1 (MQ=255)                                                                                                                             aTAACCGACATAACGGTTTCGTTGCCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTCATCGTAGGCAGCTTCAGGCTCAAACATCGTGCCTGCGCCATTTTCACGGGCGTAGATAGCCAGCACGGCAGCCAGCGGCACAGaa                      >  1:499917/1‑150 (MQ=255)                                                                                                                                 ccGACATAACGGTTTCGTTGCCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTCATCGTAGGCAGCTTCAGGCTCAAACATCGTGCCTGCGCCATTTTCACGGGCGTAGATAGCCAGCACGGCAGCCAGCGGCACAGAAACCTg                 >  2:141402/1‑151 (MQ=255)                                                                                                                                 ccGACATAACGGTTTCGTTGCCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTCATCGTAGGCAGCTTCAGGCTCAAACATCGTGCCTGCGCCATTTTCACGGGCGTAGATAGCCAGCACGGCAGCCAGCGGCACAGAAACCTg                 >  2:174316/1‑151 (MQ=255)                                                                                                                                 ccGACATAACGGTTTCGTTGCCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTCATCGTAGGCAGCTTCAGGCTCAAACATCGTGCCTGCGCCATTTTCACGGGCGTAGATAGCCAGCACGGCAGCCAGCGGCACAGAAACCTg                 >  1:249641/1‑151 (MQ=255)                                                                                                                                   gACATAACGGTTTCGTTGCCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTCATCGTAGGCAGCTTCAGGCTCAAACATCGTGCCTGCGCCATTTTCACGGGCGTAGATAGCCAGCACGGCAGCCAGCGGCACAGAAACCTGAc               <  2:36079/151‑1 (MQ=255)                                                                                                                                    aCATAACGGTTTCGTTGCCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTCATCGTAGGCAGCTTCAGGCTCAAACATCGTGCCTGCGCCATTTTCACGGGCGTAGATAGCCAGCACGGCAGCCAGCGGCACAGAAACCTGAcg              >  1:291948/1‑151 (MQ=255)                                                                                                                                        aaCGGTTTCGTTGCCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTCATCGTAGGCAGCTTCAGGCTCAAACATCGTGCCTGCGCCATTTTCACGGGCGTAGATAGCCAGCACGGCAGCCAGCGGCACAGAAACCTGACGCgg           >  2:97383/1‑150 (MQ=255)                                                                                                                                                 gTTGCCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTCATCGTAGGCAGCTTCAGGCTCAAACATCGTGCCTGCGCCATTTTCACGGGCGTAGATAGCCAGCACGGCAGCCAGCGGCACAGAAACCTGACGCGGAATGccacc  >  2:263252/1‑150 (MQ=255)                                                                                                                                                     |                                                                                                                                                  CAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGTCTGCCGATGCCTCTTCATCATTCATGATGCTGGTATCTTCATCGTAGGCAGCTTCAGGCTCAAACATCGTGCCTGCGCCATTTTCACGGGCGTAGATAGCCAGCACGGCAGCCAGCGGCACAGAAACCTGACGCGGAATGCCACC  >  NC_000913/3376255‑3376548

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑