Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	277,996	A→G	G269G (GGT→GGC)	afuC ←	CP4‑6 prophage; putative ferric transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	277,996	0	A	G	100.0%	127.9 / NA	35	G269G (GGT→GGC)	afuC	CP4‑6 prophage; putative ferric transporter subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/19);  total (16/19)

CTGCAATAATATCTCCTGCCCGTGCCATTCCACCGTCACTTCATACTGCGGCCCCATATAGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGAGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCACCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAGATATCAACGTATCCGTCGCTGAAGGTTGCCGGGAACAGGTTGGCATCGCCCATAAAGCTCGCCATAAAGCGGGAGGCGGGCTGGCGGTAAAGATCCTGC  >  NC_000913/277849‑278140                                                                                                                                                    |                                                                                                                                                 cTGCAATAATATCTCCTGCCCGTGCCATTCCACCGTCACTTCATACTGCGGCCCCATATAGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGccc                                                                                                                                               >  1:98323/1‑151 (MQ=255)    cAATAATATCTCCTGCCCGTGCCATTCCACCGTCACTTCATACTGCGGCCCCATATAGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCtgt                                                                                                                                            <  1:265317/151‑1 (MQ=255)    cAATAATATCTCCTGCCCGTGCCATTCCACCGTCACTTCATACTGCGGCCCCATATAGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCtgt                                                                                                                                            >  1:333350/1‑151 (MQ=255)        aataTCTCCTGCCCGTGCCATTCCACCGTCACTTCATACTGCGGCCCCATATAGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTAc                                                                                                                                        >  2:534676/1‑151 (MQ=255)                        gCCATTCCACCGTCACTTCATACTGCGGCCCCATATAGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGgcg                                                                                                                        >  1:283822/1‑151 (MQ=255)                        gCCATTCCACCGTCACTTCATACTGCGGCCCCATATAGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGgc                                                                                                                         <  2:97286/150‑1 (MQ=255)                             tCCACCGTCACTTCATACTGCGGCCCCATATAGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGc                                                                                                                    >  2:36591/1‑150 (MQ=255)                                aCCGTCACTTCATACTGCGGCCCCATATAGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGc                                                                                                                    >  1:177288/1‑147 (MQ=255)                                       cTTCATACTGCGGCCCCATATATGCGACATGGCGGGTCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACCCCGACCATCCCATCGCCCTGTGTACCAAAGTGCAGCGGGCGCTGCAGAGGATa                                                                                                            <  2:610357/148‑1 (MQ=255)                                         tCATACTGCGGCCCCATATAGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCg                                                                                                        <  1:493971/150‑1 (MQ=255)                                           aTACTGCGGCCCCATATAGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAg                                                                                                     <  2:133143/151‑1 (MQ=255)                                               tGCGGCCCCATATAGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAGatat                                                                                                 >  2:130724/1‑151 (MQ=255)                                                   gCCCCATATAGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAGATATCAAc                                                                                             <  1:213766/151‑1 (MQ=255)                                                         tataGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAGATATCAACGTATcc                                                                                       >  2:50380/1‑151 (MQ=255)                                                           taGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAGATATCAACGTATCCGt                                                                                     >  2:98569/1‑151 (MQ=255)                                                               cGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTATCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAGATATCAACGTATCCGTCGCt                                                                                 <  1:226646/151‑1 (MQ=255)                                                                gACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAGATATCAACGTATCCGTCGCTg                                                                                <  2:338362/151‑1 (MQ=255)                                                                   aTGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAGATATCAACGTATCCGTCGCTGAAg                                                                             <  2:65459/151‑1 (MQ=255)                                                                   aTGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAGATATCAACGTATCCGTCGCTGAAg                                                                             <  1:176271/151‑1 (MQ=255)                                                                   aTGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAGATATCAACGTATCCGTCGCTGAAg                                                                             <  1:406005/151‑1 (MQ=255)                                                                         gATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAGATATCAACGTATCCGTCGCTGAAGGTTGcc                                                                       <  1:98569/151‑1 (MQ=255)                                                                          aTCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAGATATCAACGTATCCGTCGCTGAAGGTTGCCg                                                                      >  1:615280/1‑151 (MQ=255)                                                                             aCGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAGATGTCAACGTATCCGTCGCTGAAGGTTGCCGGGa                                                                   <  1:572949/151‑1 (MQ=255)                                                                               gCAGCGCTGGCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAGATATCAACGTATCCGTCGCTGAAGGTTGCCGGGAAc                                                                 <  1:8124/151‑1 (MQ=255)                                                                                       ggCTCTCTTCGCCGCGATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAGATATCAACGTATCCGTCGCTGAAGGTTGCCGGGAACAGGTTg                                                           <  2:572740/149‑1 (MQ=255)                                                                                                    gcgATCGCTGGGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAGATATCAACGTATCCGTCGCTGAAGGTTGCCGGGAACAGGTTGGCATCGCCCATaa                                              >  1:73038/1‑149 (MQ=255)                                                                                                                   gATCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAGATATCAACGTATCCGTCGCTGAAGGTTGCCGGGAACAGGTTGGCATCGCCCATAAAGCTCGCCATAAAGCg                              <  2:121813/150‑1 (MQ=255)                                                                                                                     tCGCTTCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAGATATCAACGTATCCGTCGCTGAAGGTTGCCGGGAACAGGTTGGCATCGCCCATAAAGCTCGCCATAAAGCggg                            >  2:382106/1‑150 (MQ=255)                                                                                                                          tCCGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAGATATCAACGTATCCGTCGCTGAAGGTTGCCGGGAACAGGTTGGCATCGCCCATAAAGCTCGCCATAAAGCGGGAGGCgg                      >  1:206044/1‑151 (MQ=255)                                                                                                                           ccGGGCGCACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAGATATCAACGTATCCGTCGCTGAAGGTTGCCGGGAACAGGTTGGCATCGCCCATAAAGCTCGCCATAAAGCGGGAGGCggg                     >  2:174494/1‑151 (MQ=255)                                                                                                                               gcgcACACCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAGATATCAACGTATCCGTCGCTGAAGGTTGCCGGGAACAGGTTGGCATCGCCCATAAAGCTCGCCATAAAGCGGGAGGCGGGCTgg                 >  2:64184/1‑151 (MQ=255)                                                                                                                                    cacCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAGATATCAACGTATCCGTCGCTGAAGGTTGCCGGGAACAGGTTGGCATCGCCCATAAAGCTCGCCATAAAGCGGGAGGCGGGCTGGCGGTa            <  2:192151/151‑1 (MQ=255)                                                                                                                                    cacCGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAGATATCAACGTATCCGTCGCTGAAGGTTGCCGGGAACAGGTTGGCATCGCCCATAAAGCTCGCCATAAAGCGGGAGGCGGGCTGGCGGTa            <  2:631390/151‑1 (MQ=255)                                                                                                                                       cGACCATCCCTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAGATATCAACGTATCCGTCGCTGAAGGTTGCCGGGAACAGGTTGGCATCGCCCATAAAGCTCGCCATAAAGCGGGAGGCGGGCTGGCGGTaa           >  1:357170/1‑149 (MQ=255)                                                                                                                                              cccTTCGCCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAGATATCAACGTATCCGTCGCTGAAGGTTGCCGGGAACAGGTTGGCATCGCCCATAAAGCTCGCCATAAAGCGGGAGGCGGGCTGGCGGTAAAGATCCTGc  <  1:130727/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                 CTGCAATAATATCTCCTGCCCGTGCCATTCCACCGTCACTTCATACTGCGGCCCCATATAGGCGACATGGCGGATCACGCAGCGCTGGCTCTCTTCGCCGCGATCGCTGAGCGTGATCGCTTCCGGGCGCACACCGACCATCCCTTCACCCTGTGTACCAAAGTGCAGCGGGCGCGGCAGATGATAGCCGTAGATATCAACGTATCCGTCGCTGAAGGTTGCCGGGAACAGGTTGGCATCGCCCATAAAGCTCGCCATAAAGCGGGAGGCGGGCTGGCGGTAAAGATCCTGC  >  NC_000913/277849‑278140

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑