Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,449,100	T→C	T24A (ACG→GCG)	rplP ←	50S ribosomal subunit protein L16

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,449,100	0	T	C	100.0%	193.6 / NA	54	T24A (ACG→GCG)	rplP	50S ribosomal subunit protein L16
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/35);  total (19/35)

GGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGTACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTATTTACGGCCTTTACGCTGCTGCTTTTTAGGCTGAGCAGCCGGTTTTTCCGGTT  >  NC_000913/3448955‑3449237                                                                                                                                                  |                                                                                                                                          ggTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCt                                                                                                                                      <  1:261001/151‑1 (MQ=255)        ccGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCa                                                                                                                                <  2:323630/150‑1 (MQ=255)            gAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACcg                                                                                                                           <  2:420123/151‑1 (MQ=255)             aaCACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACcgc                                                                                                                          >  2:73310/1‑151 (MQ=255)                   cGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTAc                                                                                                                    <  1:619386/151‑1 (MQ=255)                   cGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTAc                                                                                                                    <  2:262204/151‑1 (MQ=255)                        ccAGATCTTACCTTGACGCTTAACTGCACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCt                                                                                                               >  1:37468/1‑151 (MQ=255)                         cAGATCTTACCTTGACGCTTAACTGCACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCtt                                                                                                              >  2:40579/1‑151 (MQ=255)                                  ccTTGACGCTTAACTGCACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCAttt                                                                                                     >  1:224378/1‑151 (MQ=255)                                    ttGACGCTTAACTGCACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCAtttt                                                                                                    >  1:288765/1‑150 (MQ=255)                                     tGACGCTTAACTGCACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCAtttt                                                                                                    <  1:39819/149‑1 (MQ=255)                                      gACGCTTAACTGCACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTAc                                                                                                  <  2:369154/150‑1 (MQ=255)                                         gCTTAACTGCACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGa                                                                                               >  1:63767/1‑150 (MQ=255)                                             aaCTGCACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAAttt                                                                                           <  1:442072/150‑1 (MQ=255)                                              aCTGCACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTg                                                                                         >  1:584841/1‑151 (MQ=255)                                              aCTGCACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTg                                                                                         <  1:254236/151‑1 (MQ=255)                                              aCTGCACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTg                                                                                         <  2:469487/151‑1 (MQ=255)                                                 gCACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTAc                                                                                      >  1:431754/1‑151 (MQ=255)                                                 gCACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGGCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTGTGTGCATTTTACGGACTTTTGt                                                                                        >  2:304286/1‑149 (MQ=255)                                                  cACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACg                                                                                     <  1:152308/151‑1 (MQ=255)                                                  cACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACg                                                                                     <  1:280228/151‑1 (MQ=255)                                                     gggTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCtt                                                                                  <  1:455986/151‑1 (MQ=255)                                                          aTAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGtt                                                                             >  1:456404/1‑151 (MQ=255)                                                            aGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGt                                                                           >  1:147179/1‑151 (MQ=255)                                                              cACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTaa                                                                         <  2:475876/151‑1 (MQ=255)                                                                      gctgctTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGc                                                                  <  1:490112/150‑1 (MQ=255)                                                                      gctgctTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCg                                                                 >  2:260311/1‑151 (MQ=255)                                                                      gctgctTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCg                                                                 <  2:621651/151‑1 (MQ=255)                                                                                 cTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTAt                                                      >  1:133684/1‑151 (MQ=255)                                                                                  tGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTAtt                                                     >  2:335010/1‑151 (MQ=255)                                                                                   gACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTAttt                                                    <  2:212900/151‑1 (MQ=255)                                                                                   gACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTAttt                                                    <  2:565060/151‑1 (MQ=255)                                                                                   gACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTAtt                                                     <  1:54666/150‑1 (MQ=255)                                                                                   gACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTAtt                                                     <  1:50599/150‑1 (MQ=255)                                                                                   gACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTAt                                                      <  1:40579/149‑1 (MQ=255)                                                                                   gACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCACCATaaga                                                    <  1:436697/151‑4 (MQ=255)                                                                                       ggCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTATTTACgg                                                <  2:359795/151‑1 (MQ=255)                                                                                             cAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTATTTACGGCCTTTa                                          <  2:276264/151‑1 (MQ=255)                                                                                               gacgacCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTATTTACGGCCTTTACg                                        <  2:557035/151‑1 (MQ=255)                                                                                                 cgacCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTATTTACGGCCTTTACgct                                      >  1:300177/1‑151 (MQ=255)                                                                                                     cACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTATTTACGGCCTTTACgctgctg                                  >  1:616687/1‑151 (MQ=255)                                                                                                       cGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTATTTACGGCCTTTACgctgctgct                                <  1:30472/151‑1 (MQ=255)                                                                                                           cAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTATTTACGGCCTTTACGCTGCTGCttttt                            >  2:519755/1‑151 (MQ=255)                                                                                                             aCAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTATTTACGGCCTTTACGCTGCTGCTTTTTAg                          <  2:82419/151‑1 (MQ=255)                                                                                                             aCAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTATTTACGGCCTTTACGCTGCTGCTTTTTAg                          <  1:263089/151‑1 (MQ=255)                                                                                                             aCAGCTTTCAGACCAAAGCTGTCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCATTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTATTTACTGCCTTTACGCTGCTGCTTTTTa                           <  1:515565/150‑1 (MQ=255)                                                                                                                   ttCAGACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTATTTACGGCCTTTACGCTGCTGCTTTTTAGGCTg                      <  2:62515/149‑1 (MQ=255)                                                                                                                       gACCGAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTATTTACGGCCTTTACGCTGCTGCTTTTTAGGCTGagcagc                <  2:396375/151‑1 (MQ=255)                                                                                                                           gAAGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTATTTACGGCCTTTACGCTGCTGCTTTTTAGGCTGAGCAGCCGGt            >  2:523443/1‑151 (MQ=255)                                                                                                                            aaGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTATTTACGGCCTTTACGCTGCTGCTTTTTAGGCTGAGCAGCCGGtt           >  2:374049/1‑151 (MQ=255)                                                                                                                             aGCTGCCGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTATTTACGGCCTTTACGCTGCTGCTTTTTAGGCTGAGCAGCCGGttt          <  2:189379/151‑1 (MQ=255)                                                                                                                                   cGAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTATTTACGGCCTTTACGCTGCTGCTTTTTAGGCTGAGCAGCCGGTTTTTcc      <  2:331866/149‑1 (MQ=255)                                                                                                                                    gAAGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTATTTACGGCCTTTACGCTGCTGCTTTTTAGGCTGAGCAGCCGGTTTTTCCGgt   <  1:295797/151‑1 (MQ=255)                                                                                                                                     aaGCTAACATCCGCACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTATTTACGGCCTTTACGCTGCTGCTTTTTAGGCTGAGCAGCCGGTTTTTCCGgtc  <  2:44413/151‑2 (MQ=255)                                                                                                                                                  |                                                                                                                                          GGTTTGTCCGGGAACACACGGATCCAGATCTTACCTTGACGCTTAACTGCACGGGTCATAGCACGACGTGCTGCTTCGATCTGACGGGCAGTCAGACGACCACGGCCAACAGCTTTCAGACCGAAGCTGCCGAAGCTAACATCCGTACCCTGCGCCAGACCGCGGTTACGGCCTTTGTGCATTTTACGGAATTTTGTACGCTTTGGTTGTAACATCAGCGACGCTCCTTATTTACGGCCTTTACGCTGCTGCTTTTTAGGCTGAGCAGCCGGTTTTTCCGGTT  >  NC_000913/3448955‑3449237

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑