Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,453,207 T→C T22A (ACC→GCC)  rpsJ ← 30S ribosomal subunit protein S10

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,453,2070TC95.5% 148.1 / ‑1.4 44T22A (ACC→GCC) rpsJ30S ribosomal subunit protein S10
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (19/23);  total (20/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.25e-01

GGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGAAAATGATTACTCCTCAGACCCATTACGATTGATGGGAGAGTGTAACCGT  >  NC_000913/3453069‑3453350
                                                                                                                                          |                                                                                                                                               
ggATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTgatcgat                                                                                                                                     >  2:44614/1‑151 (MQ=255)
        tACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACgat                                                                                                                             >  2:586918/1‑151 (MQ=255)
         aCTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACgatg                                                                                                                            <  1:308825/151‑1 (MQ=255)
         aCTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACgatg                                                                                                                            >  1:39194/1‑151 (MQ=255)
             aTCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATc                                                                                                                         >  2:117959/1‑150 (MQ=255)
                          ttgttgACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCa                                                                                                              <  1:476128/148‑1 (MQ=255)
                          ttgttgACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAgg                                                                                                            <  2:328799/150‑1 (MQ=255)
                          ttgttgACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAg                                                                                                             <  1:315850/149‑1 (MQ=255)
                              tgACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGAt                                                                                                       >  2:310178/1‑151 (MQ=255)
                                aCGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATAc                                                                                                     <  1:475432/151‑1 (MQ=255)
                                           gaTCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTgg                                                                                          >  2:519364/1‑151 (MQ=255)
                                               ggAACAGTGAAGGGCTCTTTGCGGGTCGGCAGCGGGGTCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAAGCGGCTACGGATTCTTTGGTTCt                                                                                      >  2:532472/2‑151 (MQ=255)
                                               agcaCAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGTATCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCt                                                                                      <  2:165886/148‑1 (MQ=255)
                                                 aaCAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGc                                                                                    <  2:194354/151‑1 (MQ=255)
                                                  aCAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGc                                                                                    >  1:333745/1‑150 (MQ=255)
                                                   cAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCAt                                                                                  <  1:50939/151‑1 (MQ=255)
                                                    aGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATg                                                                                 >  2:404665/1‑151 (MQ=255)
                                                       gAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATgaga                                                                              <  2:240083/151‑1 (MQ=255)
                                                              tctTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGc                                                                       >  1:332176/1‑151 (MQ=255)
                                                               ctTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCt                                                                      >  1:239334/1‑151 (MQ=255)
                                                                tttGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTc                                                                     >  1:446649/1‑151 (MQ=255)
                                                                 ttGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGc                                                                       <  2:277098/148‑1 (MQ=255)
                                                                  tGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTcc                                                                    >  2:165102/1‑150 (MQ=255)
                                                                         cGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTAttt                                                            <  2:531092/151‑1 (MQ=255)
                                                                         cGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTAttt                                                            <  2:598762/151‑1 (MQ=255)
                                                                              gCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATaa                                                       <  2:611752/151‑1 (MQ=255)
                                                                              gCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATaa                                                       <  2:69390/151‑1 (MQ=255)
                                                                                   aTCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGa                                                   >  2:205288/1‑150 (MQ=255)
                                                                                      ggACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGAAAAt                                               <  1:597235/151‑1 (MQ=255)
                                                                                        aCCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGAAAATGa                                             <  2:384681/151‑1 (MQ=255)
                                                                                           aCGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGAAAATGATTa                                          <  2:332169/151‑1 (MQ=255)
                                                                                             ggACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGAAAATGATTAct                                        <  2:164681/151‑1 (MQ=255)
                                                                                                     gcACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGAAAATGATTACTCCTCAGAc                                <  2:632090/151‑1 (MQ=255)
                                                                                                      cACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGAATGAGACCAGAGCTCCAATTATTTTATAAACAAAAATGATTACTCCTCAGAcc                               >  2:356305/1‑151 (MQ=255)
                                                                                                            tGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGAAAATGATTACTCCTCAGAccc                              <  2:283017/146‑1 (MQ=255)
                                                                                                            tGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGAAAATGATTACTCCTCAGAccc                              >  1:283024/1‑146 (MQ=255)
                                                                                                            tGCGCTTGGCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGAAAATGATTACTCCTCAGACCCATTAc                         >  1:549764/1‑151 (MQ=255)
                                                                                                                   ggCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGAAAATGATTACTCCTCAGACCCATTACGATTGa                   <  1:412204/150‑1 (MQ=255)
                                                                                                                   ggCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGAAAATGATTACTCCTCAGACCCATTACGATTGAt                  <  2:27940/151‑1 (MQ=255)
                                                                                                                    gCAGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGAAAATGATTACTCCTCAGACCCATTACGATTGATg                 >  1:366760/1‑151 (MQ=255)
                                                                                                                      aGTCTCGACGATTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGAAAATGATTACTCCTCAGACCCATTACGATTGATggg               >  1:124142/1‑151 (MQ=255)
                                                                                                                          tcGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGAAAATGATTACTCCTCAGACCCATTACGATTGATGGgag             >  2:237502/1‑149 (MQ=255)
                                                                                                                           cgacgaTTTCCGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGAAAATGATTACTCCTCAGACCCATTACGATTGATGGgag             <  1:169174/148‑1 (MQ=255)
                                                                                                                                    ccGCGGCTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGAAAATGATTACTCCTCAGACCCATTACGATTGATGGGAGAGTGTAACCGt  <  1:165107/150‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                               
GGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTATTTTATAAACGAAAATGATTACTCCTCAGACCCATTACGATTGATGGGAGAGTGTAACCGT  >  NC_000913/3453069‑3453350

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑