Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,537,161	A→G	Y117C (TAC→TGC)	greB →	transcript cleavage factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,537,161	0	A	G	100.0%	158.1 / NA	42	Y117C (TAC→TGC)	greB	transcript cleavage factor
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/25);  total (17/25)

GCCTGGAAAATCTCAAAATCGTCGATTACTCCCCTCAGCAGGAAGGCAAAGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTACATCTCTATCGATTCCCCGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACC  >  NC_000913/3537014‑3537303                                                                                                                                                    |                                                                                                                                               gCCTGGAAAATCTCAAAATCGTCGATTACTCCCCTCAGCAGGAAGGCAAAGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTGCa                                                                                                                                              <  2:90759/150‑1 (MQ=255)       aaaaTCTCAAAATCGTCGATTACTCCCCTCAGCAGGAAGGCAAAGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTGCATCTCTAt                                                                                                                                       <  2:346781/151‑1 (MQ=255)         aaTCTCAAAATCGTCGATTACTCCCCTCAGCAGGAAGGCAAAGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTGCATCTCTATCg                                                                                                                                     <  1:443742/151‑1 (MQ=255)           tctcAAAATCGTCGATTACTCCCCTCAGCAGGAAGGCAAAGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTGCATCTCTATCGAt                                                                                                                                   >  1:597857/1‑151 (MQ=255)              cAAAATCGTCGATTACTCCCCTCAGCAGGAAGGCAAAGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTGCATCTCTATCGATTcc                                                                                                                                >  1:102642/1‑151 (MQ=255)                        gATTACTCCCCTCAGCAGGAAGGCAAAGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTGACTCTCCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTGCATCTCTATCGATTCCCCGATTGccc                                                                                                                      <  2:141070/151‑1 (MQ=255)                         aTTACTCCCCTCAGCAGGAAGGCAAAGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTGCATCTCTATCGATTCCCCGATGGCCcg                                                                                                                     >  1:164529/1‑151 (MQ=255)                            aCTCCCCTCAGCAGGAAGGCAAAGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTGCATCTCTATCGATTCCCCGATGGCCCgcgc                                                                                                                  >  1:272597/1‑151 (MQ=255)                             cTCCCCTCAGCAGGAAGGCAAAGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTGCATCTCTATCGATTCCCCGAt                                                                                                                           <  1:33105/141‑1 (MQ=255)                             cTCCCCTCAGCAGGAAGGCAAAGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTGCATCTCTATCGATTCCCCGAt                                                                                                                           >  2:33105/1‑141 (MQ=255)                                 ccTCAGCAGGAAGGCAAAGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTGCATCTCTATCGATTCCCCGATGGCCCGCGCATTGc                                                                                                             <  1:474519/151‑1 (MQ=255)                                    cagcagGAAGGCAAAGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTGCATCTCTATCGATTCCCCGATGGCCCGCGCATTGCTg                                                                                                           <  1:578340/150‑1 (MQ=255)                                      gcagGAAGGCAAAGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTGCATCTCTATCGATTCCCCGATGGCCCGCGCATTGCTGaaa                                                                                                        <  1:522498/151‑1 (MQ=255)                                         ggAAGGCAAAGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTGCATCTCTATCGATTCCCCGATGGCCCGCGCATTGCTGaaaaaa                                                                                                     <  2:272590/151‑1 (MQ=255)                                              gCAAAGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTGCATCTCTATCGATTCCCCGATGGCCCGCGCATTGCTGAAAAAAGAAGt                                                                                                >  2:500867/1‑151 (MQ=255)                                                aaaGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTGCATCTCTATCGATTCCCCGATGGCCCGCGCATTGCTGAAAAAAGAAGTc                                                                                               <  1:583226/150‑1 (MQ=255)                                                aaaGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTGCATCTCTATCGATTCCCCGATGGCCCGCGCATTGCTGAAAAAAGAAGTCg                                                                                              <  2:124494/151‑1 (MQ=255)                                                         tttGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTGCATCTCTATCGATTCCCCGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTg                                                                                      <  2:390309/150‑1 (MQ=255)                                                           tGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTGCATCTCTATCGATTCCCCGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCg                                                                                   >  1:572126/1‑151 (MQ=255)                                                                cgTGGGTGGAGATTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTGCATCTCTATCGATTCCCCGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCggtgg                                                                               >  2:135307/1‑150 (MQ=255)                                                                     gtggAGATTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTGCATCTCTATCGATTCCCCGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAAt                                                                          >  1:170382/1‑150 (MQ=255)                                                                        gagaTTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTGCATCTCTATCGATTCCCCGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATAccc                                                                      >  1:48323/1‑151 (MQ=255)                                                                        gagaTTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTGCATCTCTATCGATTCCCCGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATAccc                                                                      <  2:70632/151‑1 (MQ=255)                                                                          gaTTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTGCATCTCTATCGATTCCCCGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCt                                                                    <  2:182261/151‑1 (MQ=255)                                                                           aTTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTGCATCTCTATCGATTCCCCGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTg                                                                   >  2:289285/1‑151 (MQ=255)                                                                                aaaCGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTGCATCTCTATCGATTCCCCGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCggg                                                              <  2:613810/151‑1 (MQ=255)                                                                                   cgacgaTGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTGCATCTCTATCGATTCCCCGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGaa                                                           <  2:161946/151‑1 (MQ=255)                                                                                    gacgaTGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTGCATCTCTATCGATTCCCCGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGa                                                            >  1:130124/1‑149 (MQ=255)                                                                                           gCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTGCATCTCTATCGATTCCCCGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTg                                                   <  2:249045/151‑1 (MQ=255)                                                                                            cGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTGCATCTCTATCGATTCCCCGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTgg                                                  >  2:310632/1‑151 (MQ=255)                                                                                             gTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTGCATCTCTATCGATTCCCCGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGt                                                 <  1:135310/151‑1 (MQ=255)                                                                                               gACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTGCATCTCTATCGATTCCCCGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTa                                                <  2:375047/150‑1 (MQ=255)                                                                                                aCTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTGCATCTCTATCGATTCCCCGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATg                                              <  2:630304/151‑1 (MQ=255)                                                                                                aCTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTGCATCTCTATCGATTCCCCGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATg                                              >  1:279277/1‑151 (MQ=255)                                                                                                      cGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTGCATCTCTATCGATTCCCCGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATg                                        <  2:406890/151‑1 (MQ=255)                                                                                                          tCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTGCATCTCTATCGATTCCCCGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTAt                                    >  2:77341/1‑151 (MQ=255)                                                                                                                ttGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTGCATCTCTATCGATTCCCCGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGt                               >  2:4552/1‑150 (MQ=255)                                                                                                                ttGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTGCATCTCTATCGATTCCCCGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGt                               <  1:309796/150‑1 (MQ=255)                                                                                                                       cTACGATGAAATTTTTGGCCGTAAAGATTGCATCTCTATCGATTCCCCGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGaa                        >  1:19895/1‑150 (MQ=255)                                                                                                                                 aTTTTTGGCCGTAAAGATTGCATCTCTATCGATTCCCCGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGaa             <  2:19895/151‑1 (MQ=255)                                                                                                                                          cGTAAAGATTGCATCTCTATCGATTCCCCGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTaa    <  1:509360/151‑1 (MQ=255)                                                                                                                                             aaaGATTGCATCTCTATCGATTCCCCGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAAcc  <  2:331924/150‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                               GCCTGGAAAATCTCAAAATCGTCGATTACTCCCCTCAGCAGGAAGGCAAAGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGCGTGACTCACCGTTTCCGTATTGTCGGCTACGATGAAATTTTTGGCCGTAAAGATTACATCTCTATCGATTCCCCGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACC  >  NC_000913/3537014‑3537303

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑