Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,612,540	T→C	W226R (TGG→CGG)	yhhT →	UPF0118 family putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,612,540	0	T	C	100.0%	114.8 / NA	32	W226R (TGG→CGG)	yhhT	UPF0118 family putative transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/16);  total (16/16)

CACATCGCGGGATTACACCGCGCACTTAAAGGCGTTTCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGTGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGTTTAATGGTGTTTACGAATGTATTCTGGTCGGCGCATTGTTTTTAGTGGTCC  >  NC_000913/3612408‑3612678                                                                                                                                     |                                                                                                                                           cacaTCGCGGGATTACACCGCGCACTTAAAGGCGTTTCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGtttt                                                                                                                          >  2:251270/1‑151 (MQ=255)       gcgGGATTACACCGCGCACTTAAAGGCGTTTCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCt                                                                                                                      >  2:132715/1‑149 (MQ=255)       gcgGGATTACACCGCGCACTTAAAGGCGTTTCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCa                                                                                                                    <  1:213143/151‑1 (MQ=255)            aTTACACCGCGCACTTAAAGGCGTTTCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTAc                                                                                                               >  1:295392/1‑151 (MQ=255)                              aGGCGTTTCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGcgcg                                                                                             <  2:192341/151‑1 (MQ=255)                                   tttCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAAt                                                                                        >  2:260258/1‑151 (MQ=255)                                     tCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAAtt                                                                                       <  1:345436/150‑1 (MQ=255)                                       gCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTcc                                                                                    <  1:573056/151‑1 (MQ=255)                                        cACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTccg                                                                                   >  1:338124/1‑151 (MQ=255)                                            aTCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGt                                                                               >  1:446185/1‑151 (MQ=255)                                                tGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGt                                                                               <  2:500954/147‑1 (MQ=255)                                                tGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGt                                                                               <  1:418834/147‑1 (MQ=255)                                                     tGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAAt                                                                      >  2:624437/1‑151 (MQ=255)                                                       aaGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATg                                                                     >  1:4736/1‑150 (MQ=255)                                                        aGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGAt                                                                   >  2:44901/1‑151 (MQ=255)                                                         gACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGAtt                                                                  <  1:555239/151‑1 (MQ=255)                                                            gCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAg                                                               <  1:252615/151‑1 (MQ=255)                                                               aCTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAgg                                                              <  2:481037/149‑1 (MQ=255)                                                               aCTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGtg                                                            >  1:590812/1‑151 (MQ=255)                                                                              aGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGTTTAATGgt                                             >  1:148006/1‑151 (MQ=255)                                                                                gtgtCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGTTTAATGgtgt                                           <  2:338117/151‑1 (MQ=255)                                                                                          ctggctggGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGTTTAATGGTGTTTACGAATGt                                 >  1:113990/1‑151 (MQ=255)                                                                                            ggctggggctggAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGTTTAATGGTGTTTACGAATGTAt                               >  2:40017/1‑151 (MQ=255)                                                                                              ctggggctggAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGTTTAATGGTGTTTACGAATGTATTc                             >  1:506967/1‑151 (MQ=255)                                                                                               tggggctggAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGTTTAATGGTGTTTACGAATGTATTCt                            >  1:365646/1‑151 (MQ=255)                                                                                                      ggAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGTTTAATGGTGTTTACGAATGTATTCTGGTCgg                      <  1:417286/150‑1 (MQ=255)                                                                                                      ggAGCTGATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGTTTAATGGTGTTTACGAATGTATTCTGGTCg                       <  2:446765/149‑1 (MQ=255)                                                                                                            gATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGTTTAATGGTGTTTACGAATGTATTCTGGTCGGCGCAtt                <  1:491355/150‑1 (MQ=255)                                                                                                            gATGGGGGTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGTTTAATGGTGTTTACGAATGTATTCTGGTCGGCGCATTg               <  1:475936/151‑1 (MQ=255)                                                                                                               gggggTGCAGTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGTTTAATGGTGTTTACGAATGTATTCTGGTCGGCGCATTGttt            >  1:294398/1‑151 (MQ=255)                                                                                                                        gTTTGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGTTTAATGGTGTTTACGAATGTATTCTGGTCGGCGCATTGTTTTTAGTGGTc   <  2:601428/151‑1 (MQ=255)                                                                                                                          ttGCGCTGATGCGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGTTTAATGGTGTTTACGAATGTATTCTGGTCGGCGCATTGTTTTTAGTGGTcc  <  2:353416/150‑1 (MQ=255)                                                                                                                                     |                                                                                                                                           CACATCGCGGGATTACACCGCGCACTTAAAGGCGTTTCGCACTATCTTGCATTGAAGACGCTACTCAGTTTATGGACAGGTGTCATCGTCTGGCTGGGGCTGGAGCTGATGGGGGTGCAGTTTGCGCTGATGTGGGCAGTACTGGCGTTTTTGCTCAACTACGTGCCCAATATCGGCGCGGTAATTTCCGCCGTACCGCCAATGATTCAGGTGCTGCTGTTTAATGGTGTTTACGAATGTATTCTGGTCGGCGCATTGTTTTTAGTGGTCC  >  NC_000913/3612408‑3612678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑