Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,005,381	A→G	S174G (AGC→GGC)	pldA →	outer membrane phospholipase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,005,381	0	A	G	100.0%	119.3 / NA	34	S174G (AGC→GGC)	pldA	outer membrane phospholipase A
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/21);  total (13/21)

CTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATGTGGTGGGTAATACTGACGATAACCCGGATATCACCAAATATATGGGTTACTACCAGCTTAAAATCGGCTATC  >  NC_000913/4005238‑4005525                                                                                                                                                |                                                                                                                                                 cTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCGGCTGGa                                                                                                                                            >  2:362939/1‑150 (MQ=255)  ttCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCGGCTGGaa                                                                                                                                           >  2:223313/1‑150 (MQ=255)    cACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCGGCTGGAACCg                                                                                                                                        <  2:586068/151‑1 (MQ=255)            cGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCCCCGATTCCCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATACCCACGCCTCTAACGGGCGTTCCGACCCGACCTCCCGCGGCTGGAACCGCCTTtat                                                                                                                                 <  2:592536/150‑1 (MQ=255)            cGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCGGCTGGAACCGCCTTtat                                                                                                                                 >  1:404717/1‑150 (MQ=255)                   ccAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCGGCTGGAACCGCCTTTATACTCGCCt                                                                                                                         >  1:256070/1‑151 (MQ=255)                                   aTTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCGGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGt                                                                                                         >  2:226632/1‑151 (MQ=255)                                       ttCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGCCGCTGCGCGATGTGGAGATGGGTTATAACCACGACTCTAACGGGCGTCCCGACCCGACCTCCCGCGCCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTaa                                                                                                       <  2:305389/149‑1 (MQ=255)                                         ccTCGGTTTTGCCACCGATTACCGTTTTGCGGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCGGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAAct                                                                                                     <  1:102663/149‑1 (MQ=255)                                                tttGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCGGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGt                                                                                              >  1:594276/1‑149 (MQ=255)                                                tttGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCGGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTa                                                                                             <  1:443334/150‑1 (MQ=255)                                                tttGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCGGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTa                                                                                             <  1:347995/150‑1 (MQ=255)                                                   gCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCGGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAg                                                                                         <  2:578298/151‑1 (MQ=255)                                                         gATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCGGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGc                                                                                   >  2:358928/1‑151 (MQ=255)                                                                gTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGTCGTTCCGACCCGACTTCCCGCGGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTa                                                                            <  2:200557/151‑1 (MQ=255)                                                                       aGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCGGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATgtggtg                                                                     >  1:199693/1‑151 (MQ=255)                                                                       aGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCGGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATgtggtg                                                                     >  1:18139/1‑151 (MQ=255)                                                                              aCGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCGGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATGTGGTGGGTAAt                                                               >  1:440043/1‑150 (MQ=255)                                                                                ggtGCGCGATGTGGAGATGGGGTATACCCACGACTCTAACGGGCGTTCCGACCCGCCCCCCCGCGGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATGTGGTGGGTAATACt                                                            <  1:498631/149‑1 (MQ=255)                                                                                gCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCGGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATGTGGTGGGTAATACt                                                            >  2:599961/1‑151 (MQ=255)                                                                                      cgATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCGGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATGTGGTGGGTAATACTGACGAt                                                      <  1:131261/151‑1 (MQ=255)                                                                                         tgtgGAGATGGGGTATAACCACGATTTTAACGGGCGTTCCGACCGGACCTCCCGCGGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATGTGGTGGGTAATACTGACGATaa                                                    <  1:53718/150‑1 (MQ=255)                                                                                                   gggTATAACCACGACTCTAACGGGCGTCCCGACCCGACCTCCCGCGGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATGTGGTGGGTAATACTGACGATAACCCGGATATCa                                         <  2:440035/151‑1 (MQ=255)                                                                                                      tataACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCGGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATGTGGTGGGTAATACTGACGATAACCCGGATATCAcc                                       <  1:385081/150‑1 (MQ=255)                                                                                                         aaCCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCGGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATGTGGTGGGTAATACTGACGATAACCCGGATATCACCAAat                                   >  2:130558/1‑151 (MQ=255)                                                                                                                     aaCGGGCGTTCCGACCCGACCTCCCGCGGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATGTGGTGGGTAATACTGACGATAACCCGGATATCACCAAATATATGGGTta                         <  2:219950/149‑1 (MQ=255)                                                                                                                          gCGTTCCGACCCGACCTCCCGCGGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATGTGGTGGGTAATACTGACGATAACCCGGATATCACCAAATATATGGGTTACTACCAg                  <  2:124789/151‑1 (MQ=255)                                                                                                                           cGTTCCGACCCGACCTCCCGCGGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATGTGGTGGGTAATACTGACGATAACCCGGATATCACCAAATATATGGGTTACTACCAg                  <  2:275688/150‑1 (MQ=255)                                                                                                                               ccgacccgacCTCCCGCGGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATGTGGTGGGTAATACTGACGATAACCCGGATATCACCAAATATATGGGTTACTACCAGCtt               <  2:184624/149‑1 (MQ=255)                                                                                                                               ccgacccgacCTCCCGCGGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATGTGGTGGGTAATACTGACGATAACCCGGATATCACCAAATATATGGGTTACTACCAGCTTa              <  2:3181/150‑1 (MQ=255)                                                                                                                                cgacccgaccTCCCGCGGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATGTGGTGGGTAATACTGACGATAACCCGGATATCACCAAATATATGGGTTACTACCAGCTTaaa            <  1:318647/151‑1 (MQ=255)                                                                                                                                 gacccgaccTCCCGCGGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATGTGGTGGGTAATACTGACGATAACCCGGATATCACCAAATATATGGGTTACTACCAGCtt               <  2:57928/147‑1 (MQ=255)                                                                                                                                   cccgaccTCCCGCGGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATGTGGTGGGTAATACTGACGATAACCCGGATATCACCAAATATATGGGTTACTACCAGCTTAAAAt          <  2:288263/150‑1 (MQ=255)                                                                                                                                          tCCCGCGGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATGTGGTGGGTAATACTGACGATAACCCGGATATCACCAAATATATGGGTTACTACCAGCTTAAAATCGGCTATc  >  2:263262/1‑151 (MQ=255)                                                                                                                                                |                                                                                                                                                 CTTCACCGTTTCGTGAAACCAACTACGAACCGCAATTGTTCCTCGGTTTTGCCACCGATTACCGTTTTGCAGGTTGGACGCTGCGCGATGTGGAGATGGGGTATAACCACGACTCTAACGGGCGTTCCGACCCGACCTCCCGCAGCTGGAACCGCCTTTATACTCGCCTGATGGCAGAAAACGGTAACTGGCTGGTAGAAGTGAAGCCGTGGTATGTGGTGGGTAATACTGACGATAACCCGGATATCACCAAATATATGGGTTACTACCAGCTTAAAATCGGCTATC  >  NC_000913/4005238‑4005525

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑