Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,229,213	C→T	L495L (CTG→TTG)	yjbB →	putative Na+/Pi‑cotransporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,229,213	0	C	T	100.0%	162.0 / NA	42	L495L (CTG→TTG)	yjbB	putative Na+/Pi‑cotransporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/21);  total (21/21)

TCTATGAGCAATTGCTCAGTAATTTAAAGCTGGCAATGTCGGTGTTCTTCTCTGGCGATGTCACCAGCGCTCGTCGTTTGCGTCGCAGCAAACATCGTTTTCGCATTCTTAATCGCCGCTATTCCCACGCCCACGTCGATCGCCTGCATCAGCAAAACGTGCAGAGCATTGAAACCAGTTCGCTACATTTAGGCTTACTGGGAGATATGCAGCGCCTGAACTCGCTGTTTTGTTCGGTGGCTTACAGTGTGCTGGAACAGCCGGATGAAGATGAAGGACGGGACG  >  NC_000913/4229070‑4229354                                                                                                                                                |                                                                                                                                              tctATGAGCAATTGCTCAGTAATTTAAAGCTGGCAATGTCGGTGTTCTTCTCTGGCGATGTCACCAGCGCTCGTCGTTTGCGTCGCAGCAAACATCGTTTTCGCATTCTTAATCGCCGCTATTCCCACGCCCACGTCGATCGCTTGCATc                                                                                                                                         >  2:361264/1‑150 (MQ=255)      gAGCAATTGCTCAGTAATTTAAAGCTGGCAATGTCGGTGTTCTTCTCTGGCGATGTCACCAGCGCTCGTCGTTTGCGTCGCAGCAAACATCGTTTTCGCATTCTTAATCGCCGCTATTCCCACGCCCACGTCGATCGCTTGCATCAGCaa                                                                                                                                    <  1:361271/150‑1 (MQ=255)              gCTCAGTAATTTAAAGCTGGCAATGTCGGTGTTCTTCTCTGGCGATGTCACCAGCGCTCGTCGTTTGCGTCGCAGCAAACATCGTTTTCGCATTCTTAATCGCCGCTATTCCCACGCCCACGTCGATCGCTTGCATCAGCAAAACGTGCa                                                                                                                            <  1:207957/150‑1 (MQ=255)                  aGTAATTTAAAGCTGGCAATGTCGGTGTTCTTCTCTGGCGATGTCACCAGCGCTCGTCGTTTGCGTCGCAGCAAACATCGTTTTCGCATTCTTAATCGCCGCTATTCCCACGCCCACGTCGATCGCTTGCATCAGCAAAACGTGCAGAGc                                                                                                                        >  1:114595/1‑150 (MQ=255)                       tttAAAGCTGGCAATGTCGGTGTTCTTCTCTGGCGATGTCACCAGCGCTCGTCGTTTGCGTCGCAGCAAACATCGTTTTCGCATTCTTAATCGCCGCTATTCCCACGCCCACGTCGATCGCTTGCATCAGCAAAACGTGCAGAGCATTGa                                                                                                                   >  2:100437/1‑150 (MQ=255)                          aaaGCTGGCAATGTCGGTGTTCTTCTCTGGCGATGTCACCAGCGCTCGTCGTTTGCGTCGCAGCAAACATCGTTTTCGCATTCTTAATCGCCGCTATTCCCTCGCCCACGTCGATCGCTTGCATCAGCAAAACGTGCAGAGCATTGaaa                                                                                                                 <  1:159019/149‑1 (MQ=255)                          aaaGCTGGCAATGTCGGTGTTCTTCTCTGGCGATGTCACCAGCGCTCGTCGTTTGCGTCGCAGCAAACATCGTTTTCGCATTCTTAATCGCCGCTATTCCCACGCCCACGTCGATCGCTTGCATCAGCAAAACGTGCAGAGCATTGAAAc                                                                                                                <  1:329652/150‑1 (MQ=255)                           aaGCTGGCAATGTCGGTGTTCTTCTCTGGCGATGTCACCAGCGCTCGTCGTTTGCGTCGCAGCAAACATCGTTTTCGCATTCTTAATCGCCGCTATTCCCACGCCCACGTCGATCGCTTGCATCAGCAAAACGTGCAGAGCATTGAAAcc                                                                                                               >  2:163490/1‑150 (MQ=255)                              cTGGCAATGTCGGTGTTCTTCTCTGGCGATGTCACCAGCGCTCGTCGTTTGCGTCGCAGCAAACATCGTTTTCGCATTCTTAATCGCCGCTATTCCCACGCCCACGTCGATCGCTTGCATCAGCAAAACGTGCAGAGCATTGAAACCAGtt                                                                                                           >  1:281429/1‑151 (MQ=255)                                ggCAATGTCGGTGTTCTTCTCTGGCGATGTCACCAGCGCTCGTCGTTTGCGTCGCAGCAAACATCGTTTTCGCATTCTTAATCGCCGCTATTCCCACGCCCACGTCGATCGCTTGCATCAGCAAAACGTGCAGAGCATTGAAACCAGtt                                                                                                           <  2:187783/149‑1 (MQ=255)                                 gCAATGTCGGTGTTCTTCTCTGGCGATGTCACCAGCGCTCGTCGTTTGCGTCGCAGCAAACATCGTTTTCGCATTCTTAATCGCCGCTATTCCCACGCCCACGTCGATCGCTTGCATCAGCAAAACGTGCAGAGCATTGAAACCAGTTCGc                                                                                                        <  2:12641/151‑1 (MQ=255)                                    aTGTCGGTGTTCTTCTCTGGCGATGTCACCAGCGCTCGTCGTTTGCGTCGCAGCAAACATCGTTTTCGCATTCTTAATCGCCGCTATTCCCACGCCCACGTCGATCGCTTGCATCAGCAAAACGTGCAGAGCATTGAAACCAGTTCGCTAc                                                                                                     <  2:213650/151‑1 (MQ=255)                                      gTCGGTGTTCTTCTCTGGCGATGTCACCAGCGCTCGTCGTTTGCGTCGCAGCAAACATCGTTTTCGCATTCTTAATCGCCGCTATTCCCACGCCCACGTCGATCGCTTGCATCAGCAAAACGTGCAGAGCATTGAAACCAGTTCGCTACAt                                                                                                   <  2:176321/151‑1 (MQ=255)                                            gtTCTTCTCTGGCGATGTCACCAGCGCTCGTCGTTTGCGTCGCAGCAAACATCGTTTTCGCATTCTTAATCGCCGCTATTCCCACGCCCACGTCGATCGCTTGCATCAGCAAAACGTGCAGAGCATTGAAACCAGTTCGCTACATTTAgg                                                                                              <  1:398306/150‑1 (MQ=255)                                            gtTCTTCTCTGGCGATGTCACCAGCGCTCGTCGTTTGCGTCGCAGCAAACATCGTTTTCGCATTCTTAATCGCCGCTATTCCCACGCCCACGTCGATCGCTTGCATCAGCAAAACGTGCAGAGCATTGAAACCAGTTCGCTACATTTAGGc                                                                                             >  1:215756/1‑151 (MQ=255)                                                      ggCGATGTCACCAGCGCTCGTCGTTTGCGTCGCAGCAAACATCGTTTTCGCATTCTTAATCGCCGCTATTCCCACGCCCACGTCGATCGCTTGCATCAGCAAAACGTGCAGAGCATTGAAACCAGTTCGCTACATTTAGGCTTACTgg                                                                                      <  1:18960/148‑1 (MQ=255)                                                          aTGTCACCAGCGCTCGTCGTTTGCGTCGCAGCAAACATCGTTTTCGCATTCTTAATCGCCGCTATTCCCACGCCCACGTCGATCGCTTGCATCAGCAAAACGTGCAGAGCATTGAAACCAGTTCGCTACATTTAGGCTTACTGGGAGatat                                                                               <  2:628386/151‑1 (MQ=255)                                                              cACCAGCGCTCGTCGTTTGCGTCGCAGCAAACATCGTTTTCGCATTCTTAATCGCCGCTATTCCCACGCCCACGTCGATCGCTTGCATCAGCAAAACGTGCAGAGCATTGAAACCAGTTCGCTACATTTAGGCTTACTGGGAGATATGc                                                                             >  1:582608/1‑149 (MQ=255)                                                              cACCAGCGCTCGTCGTTTGCGTCGCAGCAAACATCGTTTTCGCATTCTTAATCGCCGCTATTCCCACGCCCACGTCGATCGCTTGCATCAGCAAAACGTGCAGAGCATTGAAACCAGTTCGCTACATTTAGGCTTACTGGGAGATATGc                                                                             >  1:381490/1‑149 (MQ=255)                                                                     gcTCGTCGTTTGCGTCGCAGCAAACATCGTTTTCGCATTCTTAATCGCCGCTATTCCCACGCCCACGTCGATCGCTTGCATCAGCAAAACGTGCAGAGCATTGAAACCAGTTCGCTACATTTAGGCTTACTGGGAGATATGCAGCGCCTGa                                                                    <  2:412352/151‑1 (MQ=255)                                                                            gtTTGCGTCGCAGCAAACATCGTTTTCGCATTCTTAATCGCCGCTATTCCCACGCCCACGTCGATCGCTTGCATCAGCAAAACGTGCAGAGCATTGAAACCAGTTCGCTACATTTAGGCTTACTGGGAGATATGCAGCGCCTGAACTCGCt                                                             <  2:413761/151‑1 (MQ=255)                                                                              ttGCGTCGCAGCAAACATCGTTTTCGCATTCTTAATCGCCGCTATTCCCACGCCCACGTCGATCGCTTGCATCAGCAAAACGTGCAGAGCATTGAAACCAGTTCGCTACATTTAGGCTTACTGGGAGATATGCAGCGCCTGAACTCGCtt                                                            <  2:41272/150‑2 (MQ=255)                                                                                 cGTCGCAGCAAACATCGTTTTCGCATTCTTAATCGCCGCTATTCCCACGCCCACGTCGATCGCTTGCATCAGCAAAACGTGCAGAGCATTGAAACCAGTTCGCTACATTTAGGCTTACTGGGAGATATGCAGCGCCTGAACTCGCTGtttt                                                        >  2:182363/1‑151 (MQ=255)                                                                                    cGCAGCAAACATCGTTTTCGCATTCTTAATCGCCGCTATTCCCACGCCCACGTCGATCGCTTGCATCAGCAAAACGTGCAGAGCATTGAAACCAGTTCGCTACATTTAGGCTTACTGGGAGATATGCAGCGCCTGAACTCGCTGTTTTGtt                                                     >  1:381752/1‑151 (MQ=255)                                                                                    cGCAGCAAACATCGTTTTCGCATTCTTAATCGCCGCTATTCCCACGCCCACGTCGATCGCTTGCATCAGCAAAACGTGCAGAGCATTGAAACCAGTTCGCTACATTTAGGCTTACTGGGAGATATGCAGCGCCTGAACTCGCTGTTTTGtt                                                     >  2:260453/1‑151 (MQ=255)                                                                                            aCATCGTTTTCGCATTCTTAATCGCCGCTATTCCCACGCCCACGTCGATCGCTTGCATCAGCAAAACGTGCAGAGCATTGAAACCAGTTCGCTACATTTAGGCTTACTGGGAGATATGCAGCGCCTGAACTCGCTGTTTTGTTCGGTGGc                                              <  2:559006/150‑1 (MQ=255)                                                                                               tCGTTTTCGCATTCTTAATCGCCGCTATTCCCACGCCCACGTCGATCGCTTGCATCAGCAAAACGTGCAGAGCATTGAAACCAGTTCGCTACATTTAGGCTTACTGGGAGATATGCAGCGCCTGAACTCGCTGTTTTGTTCGGTGGCTTAc                                          >  1:339752/1‑151 (MQ=255)                                                                                               tCGTTTTCGCATTCTTAATCGCCGCTATCCCCACGCCCACGTCGATCGCTTGCATCAGCAAAACGTGCAGAGCATTGAAACCAGTTCGCTACATTTAGGCTTACTGGGAGATATGCAGCGCCTGAACTCGCTGTTTTGTTCGGTGGCTTAc                                          >  1:116989/1‑151 (MQ=255)                                                                                                  ttttCGCATTCTTAATCGCCGCTATTCCCACGCCCACGTCGATCGCTTGCATCAGCAAAACGTGCAGAGCATTGAAACCAGTTCGCTACATTTAGGCTTACTGGGAGATATGCAGCGCCTGAACTCGCTGTTTTGTTCGGTGGCTTACAgt                                       >  1:129298/1‑151 (MQ=255)                                                                                                        cATTCTTAATCGCCGCTATTCCCACGCCCACGTCGATCGCTTGCATCAGCAAAACGTGCAGAGCATTGAAACCAGTTCGCTACATTTAGGCTTACTGGGAGATATGCAGCGCCTGAACTCGCTGTTTTGTTCGGTGGCTTACAGTGTGCTg                                 >  1:376683/1‑151 (MQ=255)                                                                                                           tCTTAATCGCCGCTATTCCCACGCCCACGTCGATCGCTTGCATCAGCAAAACGTGCAGAGCATTGAAACCAGTTCGCTACATTTAGGCTTACTGGGAGATATGCAGCGCCTGAACTCGCTGTTTTGTTCGGTGGCTTACAGTGTGCTGGa                               <  2:150562/150‑1 (MQ=255)                                                                                                                 tCGCCGCTATTCCCACGCCCACGTCGATCGCTTGCATCAGCAAAACGTGCAGAGCATTGAAACCAGTTCGCTACATTTAGGCTTACTGGGAGATATGCAGCGCCTGAACTCGCTGTTTTGTTCGGTGGCTTACAGTGTGCTGGAACAGCCg                        >  2:632891/1‑151 (MQ=255)                                                                                                                    ccgcTATTCCCACGCCCACGTCGATCGCTTGCATCAGCAAAACGTGCAGAGCATTGAAACCAGTTCGCTACATTTAGGCTTACTGGGAGATATGCAGCGCCTGAACTCGCTGTTTTGTTCGGTGGCTTACAGTGTGCTGGAACAGCCGgat                     <  2:509947/151‑1 (MQ=255)                                                                                                                    ccgcTATTCCCACGCCCACGTCGATCGCTTGCATCAGCAAAACGTGCAGAGCATTGAAACCAGTTCGCTACATTTAGGCTTACTGGGAGATATGCAGCGCCTGAACTCGCTGTTTTGTTCGGTGGCTTACAGTGTGCTGGAACAGCCGgat                     >  2:119698/1‑151 (MQ=255)                                                                                                                            cccacgcccacgTCGATCGCTTGCATCAGCAAAACGTGCAGAGCATTGAAACCAGTTCGCTACATTTAGGCTTACTGGGAGATATGCAGCGCCTGAACTCGCTGTTTTGTTCGGTGGCTTACAGTGTGCTGGAACAGCCGgatgaagatg              <  2:582593/150‑1 (MQ=255)                                                                                                                            cccacgcccacgTCGATCGCTTGCATCAGCAAAACGTGCAGAGCATTGAAACCAGTTCGCTACATTTAGGCTTACTGGGAGATATGCAGCGCCTGAACTCGCTGTTTTGTTCGGTGGCTTACAGTGTGCTGGAACAGCCGgatga                   >  1:170072/1‑145 (MQ=255)                                                                                                                             ccacgcccacgTCGATCGCTTGCATCAGCAAAACGTGCAGAGCATTGAAACCAGTTCGCTACATTTAGGCTTACTGGGAGATATGCAGCGCCTGAACTCGCTGTTTTGTTCGGTGGCTTACAGTGTGCTGGAACAGCCGgatgaagatgac            <  2:503918/151‑2 (MQ=255)                                                                                                                              cacgcccacgTCGATCGCTTGCATCAGCAAAACGTGCAGAGCATTGAAACCAGTTCGCTACATTTAGGCTTACTGGGAGATATGCAGCGCCTGAACTCGCTGTTTTGTTCGGTGGCTTACAGTGTGCTGGAACAGCCGgatgaagatga             >  2:82687/1‑149 (MQ=255)                                                                                                                               acgcccacgTCGATCGCTTGCATCAGCAAAACGTGCAGAGCATTGAAACCAGTTCGCTACATTTAGGCTTACTGGGAGATATGCAGCGCCTGAACTCGCTGTTTTGTTCGGTGGCTTACAGTGTGCTGGAACAGCCGGATGAAGATGAAgg          <  1:565964/151‑1 (MQ=255)                                                                                                                                  cccacgTCGATCGCTTGCATCAGCAAAACGTGCAGAGCATTGAAACCAGTTCGCTACATTTAGGCTTACTGGGAGATATGCAGCGCCTGAACTCGCTGTTTTGTTCGGTGGCTTACAGTGTGCTGGAACAGCCGGATGAAGATGAAggacg       >  2:192812/1‑151 (MQ=255)                                                                                                                                   ccacgTCGATCGCTTGCATCAGCAAAACGTGCAGAGCATTGAAACCAGTTCGCTACATTTAGGCTTACTGGGAGATATGCAGCGCCTGAACTCGCTGTTTTGTTCGGTGGCTTACAGTGTGCTGGAACAGCCGGATGAAGATGAAggacgg      >  1:529511/1‑151 (MQ=255)                                                                                                                                       gTCGATCGCTTGCATCAGCAAAACGTGCAGAGCATTGAAACCAGTTCGCTACATTTAGGCTTACTGGGAGATATGCAGCGCCTGAACTCGCTGTTTTGTTCGGTGGCTTACAGTGTGCTGGAACAGCCGGATGAAGATGAAggacgggacg  <  1:397238/151‑1 (MQ=255)                                                                                                                                                |                                                                                                                                              TCTATGAGCAATTGCTCAGTAATTTAAAGCTGGCAATGTCGGTGTTCTTCTCTGGCGATGTCACCAGCGCTCGTCGTTTGCGTCGCAGCAAACATCGTTTTCGCATTCTTAATCGCCGCTATTCCCACGCCCACGTCGATCGCCTGCATCAGCAAAACGTGCAGAGCATTGAAACCAGTTCGCTACATTTAGGCTTACTGGGAGATATGCAGCGCCTGAACTCGCTGTTTTGTTCGGTGGCTTACAGTGTGCTGGAACAGCCGGATGAAGATGAAGGACGGGACG  >  NC_000913/4229070‑4229354

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑