Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,264,246	G→A	intergenic (‑15/‑68)	qorA ← / → dnaB	quinone oxidoreductase, NADPH‑dependent/replicative DNA helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,264,246	0	G	A	100.0%	100.7 / NA	26	intergenic (‑15/‑68)	qorA/dnaB	quinone oxidoreductase, NADPH‑dependent/replicative DNA helicase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/14);  total (12/14)

TCGATAAAATTGATGCCGATGGCTTTATTTTCGACCTGGATTTCATTCTCCGCCGGATCGGCAGGAGTGAACTCTACGGCTTGAAGTACTTCCGGGCCACCGTGCTTGTGAAATTCAATTCGTGTTGCCATGTGTCCTCCAGAACGTATCGTCAGGGTCTGCTTCATATGATAAAGTTTCGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAG  >  NC_000913/4264101‑4264389                                                                                                                                                  |                                                                                                                                                tCGATAAAATTGATGCCGATGGCTTTATTTTCGACCTGGATTTCATTCTCCGCCGGATCGGCAGGAGTGAACTCTACGGCTTGAAGTACTTCCGGGCCACCGTGCTTGTGAAATTCAATTCGTGTTGCCATGTGTCCTCCAGAACATATCg                                                                                                                                            >  1:116743/1‑151 (MQ=255)    aTAAAATTGATGCCGATGGCTTTATTTTCGACCTGGATTTCATTCTCCGCCGGATCGGCAGGAGTGAACTCTACGGCTTGAAGTACTTCCGGGCCACCGTGCTTGTGAAATTCAATTCGTGTTGCCATGTGTCCTCCAGAACATATCGTCa                                                                                                                                         <  2:439975/151‑1 (MQ=255)            gATGCCGATGGCTTTATTTTCGACCTGGATTTCATTCTCCGCCGGATCGGCAGGAGTGAACTCTACGGCTTGAAGTACTTCCGGGCCACCGTGCTTGTGAAATTCAATTCGTGTTGCCATGTGTCCTCCAGAACATATCGTCAGGGTCt                                                                                                                                   >  2:569893/1‑149 (MQ=255)            gATGCCGATGGCTTTATTTTCGACCTGGATTTCATTCTCCGCCGGATCGGCAGGAGTGAACTCTACGGCTTGAAGTACTTCCGGGCCACCGTGCTTGTGAAATTCAATTCGTGTTGCCATGTGTCCTCCAGAACATATCGTCAGGGTCTGc                                                                                                                                 >  1:282231/1‑151 (MQ=255)               gCCGATGGCTTTATTTTCGACCTGGATTTCATTCTCCGCCGGATCGGCAGGAGTGAACTCTACGGCTTGAAGTACTTCCGGGCCACCGTGCTTGTGAAATTCAATTCGTGTTGCCATGTGTCCTCCAGAACATATCGTCAGGGTCTGCTTc                                                                                                                              <  2:56480/151‑1 (MQ=255)                 cGATGGCTTTATTTTCGACCTGGATTTCATTCTCCGCCGGATCGGCAGGAGTGAACTCTACGGCTTGAAGTACTTCCGGGCCACCGTGCTTGTGAAATTCAATTCGTGTTGCCATGTGTCCTCCAGAACATATCGTCAGGGTCTGCtt                                                                                                                               >  2:486251/1‑148 (MQ=255)                  gATGGCTTTATTTTCGACCTGGATTTCATTCTCCGCCGGATCGGCAGGAGTGAACTCTACGGCTTGAAGTACTTCCGGGCCACCGTGCTTGTGAAATTCAATTCGTGTTGCCATGTGTCCTCCAGAACATATCGTCAGGGTCTGCTTCat                                                                                                                            >  2:549326/1‑150 (MQ=255)                           aTTTTCGACCTGGATTTCATTCTCCGCCGGATCGGCAGGAGTGAACTCTACGGCTTGAAGTACTTCCGGGCCACCGTGCTTGTGAAATTCAATTCGTGTTGCCATGTGTCCTCCAGAACATATCGTCAGGGTCTGCTTCATATGATAAAGt                                                                                                                  <  2:24759/151‑1 (MQ=255)                             tttCGACCTGGATTTCATTCTCCGCCGGATCGGCAGGAGTGAACTCTACGGCTTGAAGTACTTCCGGGCCACCGTGCTTGTGAAATTCAATTCGTGTTGCCATGTGTCCTCCAGAACATATCGTCAGGGTCTGCTTCATATGATAAAGttt                                                                                                                >  2:483489/1‑151 (MQ=255)                                cGACCTGGATTTCATTCTCCGCCGGATCGGCAGGAGTGAACTCTACGGCTTGAAGTACTTCCGGGCCACCGTGCTTGTGAAATTCAATTCGTGTTGCCATGTGTCCTCCAGAACATATCGTCAGGGTCTGCTTCATATGATAAAGTTTCg                                                                                                              >  1:98529/1‑150 (MQ=255)                                      ggATTTCATTCTCCGCCGGATCGGCAGGAGTGAACTCTACGGCTTGAAGTACTTCCGGGCCACCGTGCTTGTGAAATTCAATTCGTGTTGCCATGTGTCCTCCAGAACATATCGTCAGGGTCTGCTTCATATGATAAAGTTTCGACCCa                                                                                                         <  1:18002/149‑1 (MQ=255)                                      ggATTTCATTCTCCGCCGGATCGGCAGGAGTGAACTCTACGGCTTGAAGTACTTCCGGGCCACCGTGCTTGTGAAATTCAATTCGTGTTGCCATGTGTCCTCCAGAACATATCGTCAGGGTCTGCTTCATATGATAAAGTTTCGACCCAtt                                                                                                       <  1:57704/151‑1 (MQ=255)                                            cATTCTCCGCCGGATCGGCAGGAGTGAACTCTACGGCTTGAAGTACTTCCGGGCCACCGTGCTTGTGAAATTCAATTCGTGTTGCCATGTGTCCTCCAGAACATATCGTCAGGGTCTGCTTCATATGATAAAGTTTCGACCCATTCttt                                                                                                   <  2:368546/149‑1 (MQ=255)                                                   cgccgGATCGGCAGGAGTGAACTCTACGGCTTGAAGTACTTCCGGGCCACCGTGCTTGTGAAATTCAATTCGTGTTGCCATGTGTCCTCCAGAACATATCGTCAGGGTCTGCTTCATATGATAAAGTTTCGACCCATTCTTTATCTCGGTa                                                                                          <  2:79276/151‑1 (MQ=255)                                                        gATCGGCAGGAGTGAACTCTACGGCTTGAAGTACTTCCGGGCCACCGTGCTTGTGAAATTCAATTCGTGTTGCCATGTGTCCTCCAGAACATATCGTCAGGGTCTGCTTCATATGATAAAGTTTCGACCCATTCTTTATCTCGGTAACTcc                                                                                     <  1:537345/151‑1 (MQ=255)                                                           cGGCAGGAGTGAACTCTACGGCTTGAAGTACTTCCGGGCCACCGTGCTTGTGAAATTCAATTCGTGTTGCCATGTGTCCTCCAGAACATATCGTCAGGGTCTGCTTCATATGATAAAGTTTCGACCCATTCTTTATCTCGGTAACTCCAt                                                                                   <  2:477619/150‑1 (MQ=255)                                                               aGGAGTGAACTCTACGGCTTGAAGTACTTCCGGGCCACCGTGCTTGTGAAATTCAATTCGTGTTGCCATGTGTCCTCCAGAACATATCGTCAGGGTCTGCTTCATATGATAAAGTTTCGACCCATTCTTTATCTCGGTAACTCCATTCACt                                                                              >  1:353780/1‑151 (MQ=255)                                                                                   gAAGTACTTCCGGGCCACCGTGCTTGTGAAATTCAATTCGTGTTGCCATGTGTCCTCCAGAACATATCGTCAGGGTCTGCTTCATATGATAAAGTTTCGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCtt                                                          >  1:616645/1‑151 (MQ=255)                                                                                            ccGGGCCACCGTGCTTGTGAAATTCAATTCGTGTTGCCATGTGTCCTCCAGAACATATCGTCAGGGTCTGCTTCATATGATAAAGTTTCGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCaacaaaca                                                 <  1:448932/151‑1 (MQ=255)                                                                                                           tgtgAAATTCAATTCGTGTTGCCATGTGTCCTCCAGAACATATCGTCAGGGTCTGCTTCATATGATAAAGTTTCGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAAccc                                    <  2:332444/149‑1 (MQ=255)                                                                                                                    caattcGTGTTGCCATGTGTCCTCCAGAACATATCGTCAGGGTCTGCTTCATATGATAAAGTTTCGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCCGCGAAcgcg                          <  2:623129/150‑1 (MQ=255)                                                                                                                                 cATGTGTCCTCCAGAACATATCGTCAGGGTCTGCTTCATATGATAAAGTTTCGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGcc            >  1:427016/1‑151 (MQ=255)                                                                                                                                  aTGTGTCCTCCAGAACATATCGTCAGGGTCTGCTTCATATGATAAAGTTTCGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCg           <  2:478966/151‑1 (MQ=255)                                                                                                                                      gtCCTCCAGAACATATCGTCAGGGTCTGCTTCATATGATAAAGTTTCGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCt       >  2:462235/1‑151 (MQ=255)                                                                                                                                            cAGAACATATCGTCAGGGTCTGCTTCATATGATAAAGTTTCGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGaaa   <  2:616026/149‑1 (MQ=255)                                                                                                                                            cAGAACATATCGTCAGGGTCTGCTTCATATGATAAAGTTTCGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAg  >  2:217895/1‑150 (MQ=255)                                                                                                                                                  |                                                                                                                                                TCGATAAAATTGATGCCGATGGCTTTATTTTCGACCTGGATTTCATTCTCCGCCGGATCGGCAGGAGTGAACTCTACGGCTTGAAGTACTTCCGGGCCACCGTGCTTGTGAAATTCAATTCGTGTTGCCATGTGTCCTCCAGAACGTATCGTCAGGGTCTGCTTCATATGATAAAGTTTCGACCCATTCTTTATCTCGGTAACTCCATTCACTATGGCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAAAG  >  NC_000913/4264101‑4264389

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑