Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	295,230	G→A	T117M (ACG→ATG)	yagN ←	CP4‑6 prophage; uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	295,230	0	G	A	100.0%	124.3 / NA	32	T117M (ACG→ATG)	yagN	CP4‑6 prophage; uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/17);  total (15/17)

AACTCAGCTGTTGAAATGAGCTGTGATTGAGATGTATCCGGGAGTTTGAATGCGCTCATTTTGCCACCTCAGTGATTGAGATATTGGCAAAGAAAGCTTTAAGCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACGTTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTGATTTAGCCGTACTGTGATGCGATCTGGATGCCTGATTTTTACATGACATCAGACGCGCGAGAAAGTTAGTAGCATTTTGCA  >  NC_000913/295084‑295374                                                                                                                                                   |                                                                                                                                                 aaCTCAGCTGTTGAAATGAGCTGTGATTGAGATGTATCCGGGAGTTTGAATGCGCTCATTTTGCCACCTCAGTGATTGAGATATTGGCAAAGAAAGCTTTAAGCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACAtt                                                                                                                                                <  2:307380/149‑1 (MQ=255)       gCTGTTGAAATGAGCTGTGATTGAGATGTATCCGGGAGTTTGAATGCGCTCATTTTGCCACCTCAGTGATTGAGATATTGGCAAAGAAAGCTTTAAGCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCtc                                                                                                                                        <  2:173947/151‑1 (MQ=255)         tGTTGAAATGAGCTGTGATTGAGATGTATCCGGGAGTTTGAATGCGCTCATTTTGCCACCTCAGTGATTGAGATATTGGCAAAGAAAGCTTTAAGCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCtcgg                                                                                                                                      >  2:12559/1‑151 (MQ=255)             gAAATGAGCTGTGATTGAGATGTATCCGGGAGTTTGAATGCGCTCATTTTGCCACCTCAGTGATTGAGATATTGGCAAAGAAAGCTTTAAGCATTATTGAATTAGTAGGGGAAGTAATGAAGGCAGAAGGAAACATTTCGGGCtcggggtc                                                                                                                                  >  2:373942/1‑151 (MQ=255)               aaTGAGCTGTGATTGAGATGTATCCGGGAGTTTGAATGCGCTCATTTTGCCACCTCAGTGATTGAGATATTGGCAAAGAAAGCTTTAAGCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCtcggggtcgg                                                                                                                                >  2:574982/1‑151 (MQ=255)                              agaTGTATCCGGGAGTTTGAATGCGCTCATTTTGCCACCTCAGTGATTGAGATATTGGCAAAGAAAGCTTTAAGCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCAc                                                                                                                 <  1:245823/151‑1 (MQ=255)                               gaTGTATCCGGGAGTTTGAATGCGCTCATTTTGCCACCTCAGTGATTGAGATATTGGCAAAGAAAGCTTTAAGCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCAcc                                                                                                                >  1:260684/1‑151 (MQ=255)                                    aTCCGGGAGTTTGAATGCGCTCATTTTGCCACCTCAGTGATTGAGATATTGGCAAAGAAAGCTTTAAGCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAAt                                                                                                           <  2:11398/151‑1 (MQ=255)                                                gAATGCGCTCATTTTGCCACCTCAGTGATTGAGATATTGGCAAAGAAAGCTTTAAGCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCTCGTGGTCGGGGTCCCTACCATCACCTGAATCTGATGATAAgg                                                                                               <  1:373949/151‑1 (MQ=255)                                                       cTCATTTTGCCACCTCAGTGATTGAGATATTGGCAAAGAAAGCTTTAAGCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACtttt                                                                                        >  2:469667/1‑151 (MQ=255)                                                             ttGCCACCTCAGTGATTGAGATATTGGCAAAGAAAGCTTTAAGCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTg                                                                                   <  1:569192/150‑1 (MQ=255)                                                             ttGCCACCTCAGTGATTGAGATATTGGCAAAGAAAGCTTTAAGCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTg                                                                                   >  2:544238/1‑150 (MQ=255)                                                                      cAGTGATTGAGATATTGGCAAAGAAAGCTTTAAGCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTGATTTAGCCg                                                                          <  2:330905/150‑1 (MQ=255)                                                                       aGTGATTGAGATATTGGCAAAGAAAGCTTTAAGCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTGATTTAGCCGt                                                                         >  1:311268/1‑150 (MQ=255)                                                                              gagaTATTGGCAAAGAAAGCTTTAAGCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTGATTTAGCCGTACTGTGAt                                                                 >  2:579115/1‑151 (MQ=255)                                                                               agaTATTGGCAAAGAAAGCTTTAAGCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTGATTTAGCCGTACTGTGATg                                                                >  2:41402/1‑151 (MQ=255)                                                                                           agaaagCTTTAAGCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTGATTTAGCCGTACTGTGATGCGATCTGGATGc                                                    >  1:26388/1‑151 (MQ=255)                                                                                                     aaGCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTGATTTAGCCGTACTGTGATGCGATCTGGATGCCTGATTTTTa                                          <  2:567508/151‑1 (MQ=255)                                                                                                         attattGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTGATTTAGCCGTACTGTGATGCGATCTGGATGCCTGATTTTTACATg                                      <  2:311261/151‑1 (MQ=255)                                                                                                            attGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTGATTTAGCCGTACTGTGATGCGATCTGGATGCCTGATTTTTACATGa                                     >  1:242642/1‑149 (MQ=255)                                                                                                               gAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTGATTTAGCCGTACTGTGATGCGATCTGGATGCCTGATTTTTACATGACATc                                 >  1:374188/1‑150 (MQ=255)                                                                                                               gAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTGATTTAGCCGTACTGTGATGCGATCTGGATGCCTGATTTTTACATGACATc                                 <  2:195021/150‑1 (MQ=255)                                                                                                               gAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTGATTTAGCCGTACTGTGATGCGATCTGGATGCCTGATTTTTACATGACATc                                 <  1:497646/150‑1 (MQ=255)                                                                                                                    agtagGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTGATTTAGCCGTACTGTGATGCGATCTGGATGCCTGATTTTTACATGACATCAGAcgc                           <  1:613547/151‑1 (MQ=255)                                                                                                                    agtagGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTGATTTAGCCGTACTGTGATGCGATCTGGATGCCTGATTTTTACATGACATCAGAcgc                           <  1:45330/151‑1 (MQ=255)                                                                                                                    agtagGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTGATTTAGCCGTACTGTGATGCGATCTGGATGCCTGATTTTTACATGACATCAGAcg                            <  1:529955/150‑1 (MQ=255)                                                                                                                     gtagGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTGATTTAGCCGTACTGTGATGCGATCTGGATGCCTGATTTTTACATGACATCAGAcgcg                          >  2:36139/1‑151 (MQ=255)                                                                                                                       agGGGTAGTAATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTGATTTAGCCGTACTGTGATGCGATCTGGATGCCTGATTTTTACATGACATCAGAcgcgcg                        <  2:533458/151‑1 (MQ=255)                                                                                                                           gtagtaATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTGATTTAGCCGTACTGTGATGCGATCTGGATGCCTGATTTTTACATGACATCAGACGCGCGAGaa                    <  1:28633/151‑1 (MQ=255)                                                                                                                               taATGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTGATTTAGCCGTACTGTGATGCGATCTGGATGCCTGATTTTTACATGACATCAGACGCGCGAGAAAGtt                >  2:621011/1‑151 (MQ=255)                                                                                                                                  tGAAGGCAGAAGGAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTGATTTAGCCGTACTGTGATGCGATCTGGATGCCTGATTTTTACATGACATCAGACGCGCGAGAAAGTtagt             >  1:625851/1‑151 (MQ=255)                                                                                                                                             ggAAACATTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTGATTTAGCCGTACTGTGATGCGATCTGGATGCCTGATTTTTACATGACATCAGACGCGCGAGAAAGTTAGTAGCATTTTGCa  <  2:434769/151‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                 AACTCAGCTGTTGAAATGAGCTGTGATTGAGATGTATCCGGGAGTTTGAATGCGCTCATTTTGCCACCTCAGTGATTGAGATATTGGCAAAGAAAGCTTTAAGCATTATTGAATTAGTAGGGGTAGTAATGAAGGCAGAAGGAAACGTTTCGGGCTCGGGGTCGGGGTCACTACCATCACCTGAATCTGATGATAAGGCACTTTTAGCTGATTTAGCCGTACTGTGATGCGATCTGGATGCCTGATTTTTACATGACATCAGACGCGCGAGAAAGTTAGTAGCATTTTGCA  >  NC_000913/295084‑295374

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑