Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,615,100	A→G	C148C (TGT→TGC)	yjjW ←	putative pyruvate formate lyase activating enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,615,100	0	A	G	100.0%	107.0 / NA	32	C148C (TGT→TGC)	yjjW	putative pyruvate formate lyase activating enzyme
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/14);  total (18/14)

CTGCGCTTAATCTGCTGATTATCGCGTCCGGTGAGTTGTTGATGACATTCGCTCCCCCACGCTTTGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTC  >  NC_000913/4614954‑4615246                                                                                                                                                   |                                                                                                                                                   ctgcGCTTAATCTGCTGATTATCGCGTCCGGTGAGTTGTTGATGACATTCGCTCCCCCACGCTTTGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACcaggcagg                                                                                                                                                >  2:413429/1‑151 (MQ=255)            ctgctgATTATCGCGTCCGGTGAGTTGTTGATGACATTCGCTCCCCCACGCTTTGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGGCAGGTGAGATGgcgc                                                                                                                                     >  2:627854/1‑151 (MQ=255)                        gcgTCCGGTGAGTTGTTGATGACATTCGCTCCCCCACGCTTTGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGGCAGGTGAGATGGCGCAGTTGCGGATCg                                                                                                                         >  2:216539/1‑151 (MQ=255)                          gTCCGGTGAGTTGTTGATGACATTCGCTCCCCCACGCTTTGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGGCAGGTGAGATGGCGCAGTTGCGGATCGtt                                                                                                                       >  1:122910/1‑151 (MQ=255)                          gTCCGGTGAGTTGTTGATGACATTCGCTCCCCCACGCTTTGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGGCAGGTGAGATGGCGCAGTTGCGGATCGtt                                                                                                                       >  1:575804/1‑151 (MQ=255)                           tCCGGTGAGTTGTTGATGACATTCGCTCCCCCACGCTTTGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGGCAGGTGAGATTGCGCAGTTGCGGATCGt                                                                                                                        <  1:142838/149‑1 (MQ=255)                           tCCGGTGAGTTGTTGATGACATTCGCTCCCCCACGCTTTGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGGCAGGTGAGATGGCGCAGTTGCGGATCGttt                                                                                                                      >  2:71464/1‑151 (MQ=255)                           tCCGGTGAGTTGTTGATGACATTCGCTCCCCCACGCTTTGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGGCAGGTGAGATGGCGCAGTTGCGGATCGttt                                                                                                                      >  1:236478/1‑151 (MQ=255)                            ccGGTGAGTTGTTGATGACATTCGCTCCCCCACGCTTTGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGGCAGGTGAGATGGCGCAGTTGCGGATCGttt                                                                                                                      <  2:476039/150‑1 (MQ=255)                                      gttgATGACATTCGCTCCCCCACGCTTTGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGGCAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGt                                                                                                           >  1:271422/1‑151 (MQ=255)                                         gatgaCATTCGCTCCCCCACGCTTTGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGGCAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTaa                                                                                                         <  2:122986/150‑1 (MQ=255)                                             aCATTCGCTCCCCCACGCTTTGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGGCAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAgc                                                                                                    <  1:627902/151‑1 (MQ=255)                                              cATTCGCTCCCCCACGCTTTGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGGCAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAgcg                                                                                                   <  2:221623/151‑1 (MQ=255)                                                    cTCCCCCACGCTTTGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGGCAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGccacc                                                                                              >  1:338354/1‑150 (MQ=255)                                                    cTCCCCCACGCTTTGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGGCAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGccacc                                                                                              >  2:143995/1‑150 (MQ=255)                                                     tCCCCCACGCTTTGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGGCAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCcaccac                                                                                            >  1:139209/1‑151 (MQ=255)                                                     tCCCCCACGCTTTGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGGCAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCcaccac                                                                                            >  1:582959/1‑151 (MQ=255)                                                              cTTTGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGGCAGGGGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACgg                                                                                      >  2:240859/1‑148 (MQ=255)                                                                       cGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGGCAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGt                                                                             <  1:413436/148‑1 (MQ=255)                                                                       cGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGGCAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCg                                                                           <  1:576624/150‑1 (MQ=255)                                                                            aTTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGGCAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCtt                                                                     <  2:376734/151‑1 (MQ=255)                                                                              tGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGGCAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTc                                                                    >  1:594892/1‑150 (MQ=255)                                                                                cgcCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGGCAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCAcc                                                                 <  2:566770/151‑1 (MQ=255)                                                                                                  gCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGGCAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATccc                                               >  1:180712/1‑151 (MQ=255)                                                                                                        ttccccAGCCGTTTTCGCTCAACATGCCGTTACTGTCCCCCAGGCAGGTGAGAGGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGa                                          <  1:309104/147‑1 (MQ=255)                                                                                                        ttccccAGCCGGTTTCGCTAAACATGCCGTTACTGTCCCCCAGGCAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGa                                          <  1:298553/147‑1 (MQ=255)                                                                                                                    tttCGCTCAACATGCCGTTACTGTCCACCAGGCAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGc                             >  2:618076/1‑151 (MQ=255)                                                                                                                    tttCCCTCAACATGCCGTTACTGTCCCCCAGGCAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGAAAGCATTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTg                              <  1:35250/150‑1 (MQ=255)                                                                                                                                 gCCGTTACTGTCCACCAGGCAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATgg                >  1:596860/1‑151 (MQ=255)                                                                                                                                     ttACTGTCCACCAGGCAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTa             >  2:90420/1‑150 (MQ=255)                                                                                                                                          gTCCACCAGGCAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACt       >  2:250089/1‑151 (MQ=255)                                                                                                                                            ccaccaGGCAGGTGAGTTGGCGCATTTGCGGATCGTTTTTGATAGCATTAAACACCGCCCCCACAAGCGGCGGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTc     <  2:161319/151‑1 (MQ=255)                                                                                                                                               ccaGGCAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCCCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTc  <  2:594877/151‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                   CTGCGCTTAATCTGCTGATTATCGCGTCCGGTGAGTTGTTGATGACATTCGCTCCCCCACGCTTTGAGATCGAGCATTGCGCCGTCGCACACCGGGAGCAATTTTTCCCAGCCGGTTTCGCTCAACATGCCGTTACTGTCCACCAGACAGGTGAGATGGCGCAGTTGCGGATCGTTTTTGATAGCAGTAAACAGCGCCACCACAAACGGCAGCTGGGTCGTGGCTTCACCGCCACTCACCGTTATCCCTTCGATAAACAGCACTGCTTTGCGGACATGGCTAAGCACTTCGTC  >  NC_000913/4614954‑4615246

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑