Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,631,960	C→T	P410S (CCG→TCG)	slt →	lytic murein transglycosylase, soluble

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,631,960	0	C	T	100.0%	154.6 / NA	39	P410S (CCG→TCG)	slt	lytic murein transglycosylase, soluble
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/16);  total (23/16)

GAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGCCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAAT  >  NC_000913/4631816‑4632095                                                                                                                                                 |                                                                                                                                        gaagctgaagcAAAAGAGATTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGgaga                                                                                                                                   <  2:26136/151‑1 (MQ=255)    gctgaagcAAAAGAGATTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATgg                                                                                                                                >  2:144288/1‑151 (MQ=255)            aaaaGAGATTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTg                                                                                                                        >  2:614225/1‑151 (MQ=255)                gagaTTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGcgcg                                                                                                                    >  1:244582/1‑151 (MQ=255)                 agaTTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGcgcg                                                                                                                    >  1:380525/1‑150 (MQ=255)                  gaTTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGcgcg                                                                                                                    >  2:2964/1‑149 (MQ=255)                     tttGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTg                                                                                                               >  1:619681/1‑151 (MQ=255)                                    gCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAAtctc                                                                                                <  1:235504/151‑1 (MQ=255)                                         aGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGata                                                                                            >  2:134428/1‑150 (MQ=255)                                            gTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATAc                                                                                        <  2:173603/151‑1 (MQ=255)                                              ggTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACcg                                                                                      >  1:473281/1‑151 (MQ=255)                                                 ttCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACcgcgc                                                                                   >  2:467775/1‑151 (MQ=255)                                                 ttCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACcgcgc                                                                                   >  2:385210/1‑151 (MQ=255)                                                 ttCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACcgcgc                                                                                   >  2:76535/1‑151 (MQ=255)                                                    tACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGt                                                                                 >  1:376285/1‑150 (MQ=255)                                                       ccGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCg                                                                             <  2:498898/151‑1 (MQ=255)                                                         gATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAg                                                                           <  1:611517/151‑1 (MQ=255)                                                            ggTTGCAGCACAACGCATCGGCGAAGAGTAGGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTg                                                                         <  2:348053/150‑1 (MQ=255)                                                                    cacaACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCt                                                                <  2:376278/151‑1 (MQ=255)                                                                       aaCGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGTCAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGGGTGCGCGAGTTGATGTACTGGAATCTCGATAATGCCGCGCGTAGCGAGTGGGCCCATCTGGt                                                             <  2:228056/151‑1 (MQ=255)                                                                        aCGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTg                                                            >  1:527172/1‑151 (MQ=255)                                                                        aCGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTg                                                            <  1:109443/151‑1 (MQ=255)                                                                                 cGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAg                                                   <  1:137060/151‑1 (MQ=255)                                                                                       gTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCaaa                                              >  2:137159/1‑150 (MQ=255)                                                                                         aTGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAAc                                           >  1:609340/1‑151 (MQ=255)                                                                                           gAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACag                                         <  1:449887/151‑1 (MQ=255)                                                                                              cTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCGAGTCAAAAACAGAGc                                      >  2:218021/1‑151 (MQ=255)                                                                                              cTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGc                                      >  2:85203/1‑151 (MQ=255)                                                                                               tGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGc                                      >  1:592212/1‑150 (MQ=255)                                                                                                 aaGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAgg                                   <  1:132665/151‑1 (MQ=255)                                                                                                  aGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGc                                  >  1:583367/1‑151 (MQ=255)                                                                                                      tGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCa                               >  2:17971/1‑150 (MQ=255)                                                                                                      tGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCa                               <  2:609593/150‑1 (MQ=255)                                                                                                           aaGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGgc                         >  2:625179/1‑151 (MQ=255)                                                                                                            aGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGgcg                        <  2:468832/151‑1 (MQ=255)                                                                                                                 cgcAGAATGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGt                    >  2:212920/1‑150 (MQ=255)                                                                                                                      aaTGTTGACAGCGCCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCtt              >  1:83708/1‑151 (MQ=255)                                                                                                                                 cgcCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCaa   <  2:256694/151‑1 (MQ=255)                                                                                                                                  gcCCTGACTCAGGGGTCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAAt  <  1:371038/151‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                        GAAGCTGAAGCAAAAGAGATTTTGCATCAACTCATGCAACAGCGTGGTTTCTACCCGATGGTTGCAGCACAACGCATCGGCGAAGAGTATGAGCTGAAGATTGATAAAGCGCCGCAGAATGTTGACAGCGCCCTGACTCAGGGGCCGGAGATGGCGCGCGTGCGCGAGTTGATGTACTGGAATCTCGATAATACCGCGCGTAGCGAGTGGGCCAATCTGGTGAAGAGCAAGTCAAAAACAGAGCAGGCTCAACTGGCGCGGTATGCTTTCAACAACCAAT  >  NC_000913/4631816‑4632095

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑