Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	320,494	G→A	G90S (GGC→AGC)	rclR →	reactive chlorine species (RCS)‑specific activator of the rcl genes

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	320,494	0	G	A	100.0%	138.1 / NA	36	G90S (GGC→AGC)	rclR	reactive chlorine species (RCS)‑specific activator of the rcl genes
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/15);  total (21/15)

AACGCAAGGAGCGGCGAAGCTGGAAATGCCGACGGGGGAGATTTTTACATTACGCCCGGGAAATGTGGTCCTGCTACCACAAAATTCAGCTCATCGCCTGAGTCATGTCGATAATGAATCGACCTGTATTGTCTGCGGCACTCTTCGGTTGCAACATTCGGCGCGTTATTTTTTAACGTCTTTGCCGGAAACGCTGTTTTTAGCACCGGTTAACCACAGCGTGGAATATAACTGGCTGAGGGAGGCGATCCCGTTTTTACAACAGGAATCCAGATCGGCAAT  >  NC_000913/320358‑320639                                                                                                                                         |                                                                                                                                                  aaCGCAAGGAGCGGCGAAGCTGGAAATGCCGACGGGGGAGATTTTTACATTACGCCCGGGAAATGTGGTCCTGCTACCACAAAATTCAGCTCATCGCCTGAGTCATGTCGATAATGAATCGACCTGTATTGTCTGCAGCACTCTTCGGTTg                                                                                                                                     <  1:209813/151‑1 (MQ=255)  aCGCAAGGAGCGGCGAAGCTGGAAATGCCGACGGGGGAGATTTTTACATTACGCCCGGGAAATGTGGTCCTGCTACCACAAAATTCAGCTCATCGCCTGAGTCATGTCGATAATGAATCGACCTGTATTGTCTGCAGCACTCTTCGGTTGc                                                                                                                                    >  2:302914/1‑151 (MQ=255)   cGCAAGGAGCGGCGAAGCTGGAAATGCCGACGGGGGAGATTTTTACATTACGCCCGGGAAATGTGGTCCTGCTACCACAAAATTCAGCTCATCGCCTGAGTCATGTCGATAATGAATCGACCTGTATTGTCTGCAGCACTCTTCGGTTGc                                                                                                                                    >  1:518668/1‑150 (MQ=255)      aaGGAGCGGCGAAGCTGGAAATGCCGACGGGGGAGATTTTTACATTACGCCCGGGAAATGTGGTCCTGCTACCACAAAATTCAGCTCATCGCCTGAGTCATGTCGATAATGAATCGACCTGTATTGTCTGCAGCACTCTTCGGTTGCAAc                                                                                                                                 >  1:282279/1‑150 (MQ=255)       aGGAGCGGCGAAGCTGGAAATGCCAACGGGGGAGATTTTTACATTACGCCCGGGAAATGTGGTCCTGCTACCACAAAATTCAGCTCATCGCCTGAGTCATGTCGATAATGAATCGACCTGTATTGTCTGCAGCACTCTTCGGTTGCAACAt                                                                                                                               <  1:628864/151‑1 (MQ=255)        ggAGCGGCGAAGCTGGAAATGCCGACGGGGGAGATTTTTACATTACGCCCGGGAAATGTGGTCCTGCTACCACAAAATTCAGCTCATCGCCTGAGTCATGTCGATAATGAATCGACCTGTATTGTCTGCAGCACTCTTCGGTTGCAACAtt                                                                                                                              <  2:170343/151‑1 (MQ=255)         gAGCGGCGAAGCTGGAAATGCCGACGGGGGAGATTTTTACATTACGCCCGGGAAATGTGGTCCTGCTACCACAAAATTCAGCTCATCGCCTGAGTCATGTCGATAATGAATCGACCTGTATTGTCTGCAGCACTCTTCGGTTGCAACATTc                                                                                                                             <  2:311027/151‑1 (MQ=255)                          aTGCCGACGGGGGAGATTTTTACATTACGCCCGGGAAATGTGGTCCTGCTACCACAAAATTCAGCTCATCGCCTGAGTCATGTCGATAATGAATCGACCTGTATTGTCTGCAGCACTCTTCGGTTGCAACATTCGGCGCGTTATTTTTTaa                                                                                                            <  1:207821/151‑1 (MQ=255)                            gCCGACGGGGGAGATTTTTACATTACGCCCGGGAAATGTGGTCCTGCTACCACAAAATTCAGCTCATCGCCTGAGTCATGTCGATAATGAATCGACCTGTATTGTCTGCAGCACTCTTCGGTTGCAACATTCGGCGCGTTATTTTTTAACg                                                                                                          <  2:300155/151‑1 (MQ=255)                              cGACGGGGGAGATTTTTACATTACGCCCGGGAAATGTGGTCCTGCTACCACAAAATTCAGCTCATCGCCTGAGTCATGTCGATAATGAATCGACCTGTATTGTCTGCAGCACTCTTCGGTTGCAACATTCGGCGCGTTATTTTTTAACGTc                                                                                                        >  2:551108/1‑151 (MQ=255)                                aCGGGGGAGATTTTTACATTACGCCCGGGAAATGTGGTCCTGCTACCACAAAATTCAGCTCATCGCCTGAGTCATGTCGATAATGAATCGACCTGTATTGTCTGCAGCACTCTTCGGTTGCAACATTCGGCGCGTTATTTTTTAACGTCtt                                                                                                      >  1:223506/1‑151 (MQ=255)                                    gggAGATTTTTACATTACGCCCGGGAAATGTGGTCCTGCTACCACAAAATTCAGCTCATCGCCTGAGTCATGTCGATAATGAATCGACCTGTATTGTCTGCAGCACTCTTCGGTTGCAACATTCGGCGCGTTATTTTTTAACGTCTTTGcc                                                                                                  >  2:56068/1‑151 (MQ=255)                                        gaTTTTTACATTACGCCCGGGAAATGTGGTCCTGCTACCACAAAATTCAGCTCATCGCCTGAGTCATGTCGATAATGAATCGACCTGTATTGTCTGCAGCACTCTTCGGTTGCAACATTCGGCGCGTTATTTTTTAACGTCTTTGCCGGa                                                                                               >  2:101870/1‑150 (MQ=255)                                           ttttACATTACGCCCGGGAAATGTGGTCCTGCTACCACAAAATTCAGCTCATCGCCTGAGTCATGTCGATAATGAATCGACCTGTATTGTCTGCAGCACTCTTCGGTTGCAACATTCGGCGCGTTATTTTTTAACGTCTTTGCCGGAAACg                                                                                           >  1:128995/1‑151 (MQ=255)                                             ttACATTACGCCCGGGAAATGTGGTCCTGCTACCACAAAATTCAGCTCATCGCCTGAGTCATGTCGATAATGAATCGACCTGTATTGTCTGCAGCACTCTTCGGTTGCAACATTCGGCGCGTTATTTTTTAACGTCTTTGCCGGAAACGCt                                                                                         >  1:148148/1‑151 (MQ=255)                                                 aTTACGCCCGGGAAATGTGGTCCTGCTACCACAAAATTCAGCTCATCGCCTGAGTCATGTCGATAATGAATCGACCTGTATTGTCTGCAGCACTCTTCGGTTGCAACATTCGGCGCGTTATTTTTTAACGTCTTTGCCGGAAACGCTGttt                                                                                     >  1:61831/1‑151 (MQ=255)                                                           ggAAATGTGGTCCTGCTACCACAAAATTCAGCTCATCGCCTGAGTCATGTCGATAATGAATCGACCTGTATTGTCTGCAGCACTCTTCGGTTGCAACATTCGGCGCGTTATTTTTTAACGTCTTTGCCGGAAACGCTGTTTTTAGCACCgg                                                                           >  2:603010/1‑151 (MQ=255)                                                              aaTGTGGTCCTGCTACCACAAAATTCAGCTCATCGCCTGAGTCATGTCGATAATGAATCGACCTGTATTGTCTGCAGCACTCTTCGGTTGCAACATTCGGCGCGTTATTTTTTAACGTCTTTGCCGGAAACGCTGTTTTTAGCACCGGtt                                                                         >  2:166055/1‑150 (MQ=255)                                                              aaTGTGGTCCTGCTACCACAAAATTCAGCTCATCGCCTGAGTCATGTCGATAATGAATCGACCTGTATTGTCTGCAGCACTCTTCGGTTGCAACATTCGGCGCGTTATTTTTTAACGTCTTTGCCGGAAACGCTGTTTTTAGCACCGGTTa                                                                        <  2:435799/151‑1 (MQ=255)                                                                tgtgGTCCTGCTACCACAAAATTCAGCTCATCGCCTGAGTCATGTCGATAATGAATCGACCTGTATTGTCTGCAGCACTCTTCGGTTGCAACATTCGGCGCGTTATTTTTTAACGTCTTTGCCGGAAACGCTGTTTTTAGCACCGGTTAAc                                                                      >  2:391545/1‑151 (MQ=255)                                                                        tGCTACCACAAAATTCAGCTCATCGCCTGAGTCATGTCGATAATGAATCGACCTGTATTGTCTGCAGCACTCTTCGGTTGCAACATTCGGCGCGTTATTTTTTAACGTCTTTGCCGGAAACGCTGTTTTTAGCACCGGTTAACCACAGCGt                                                              >  2:555966/1‑151 (MQ=255)                                                                         gCTACCACAAAATTCAGCTCATCGCCTGAGTCATGTCGATAATGAATCGACCTGTATTGTCTGCAGCACTCTTCGGTTGCAACATTCGGCGCGTTATTTTTTAACGTCTTTGCCGGAAACGCTGTTTTTAGCACCGGTTAACCACAGCGt                                                              <  1:204841/150‑1 (MQ=255)                                                                          cTACCACAAAATTCAGCTCATCGCCTGAGTCATGTCGATAATGAATCGACCTGTATTGTCTGCAGCACTCTTCGGTTGCAACATTCGGCGCGTTATTTTTTAACGTCTTTGCCGGAAACGCTGTTTTTAGCACCGGTTAACCACAGCGTgg                                                            >  1:131969/1‑151 (MQ=255)                                                                                           tCATCGCCTGAGTCATGTCGATAATGAATCGACCTGTATTGTCTGCAGCACTCTTCGGTTGCAACATTCGGCGCGTTATTTTTTAACGTCTTTGCCGGAAACGCTGTTTTTAGCACCGGTTAACCACAGCGTGGAATATAACTGGCTgagg                                           >  2:90390/1‑151 (MQ=255)                                                                                            cATCGCCTGAGTCATGTCGATAATGAATCGACCTGTATTGTCTGCAGCACTCTTCGGTTGCAACATTCGGCGCGTTATTTTTTAACGTCTTTGCCGGAAACGCTGTTTTTAGCACCGGTTAACCACAGCGTGGAATATAACTGGCTgaggg                                          <  1:526685/151‑1 (MQ=255)                                                                                               cGCCTGAGTCATGTCGATAATGAATCGACCTGTATTGTCTGCAGCACTCTTCGGTTGCAACATTCGGCGCGTTATTTTTTAACGTCTTTGCCGGAAACGCTGTTTTTAGCACCGGTTAACCACAGCGTGGAATATAACTGGCTgagggag                                        <  2:395214/150‑1 (MQ=255)                                                                                                gCCTGAGTCATGTCGATAATGAATCGACCTGTATTGTCTGCAGCACTCTTCGGTTGCAACATTCGGCGCGTTATTTTTTAACGTCTTTGCCGGAAACGCTGTTTTTAGCACCGGTTAACCACAGCGTGGAATATAACTGGCTGAGGGAGGc                                      <  2:26795/151‑1 (MQ=255)                                                                                                gCCTGAGTCATGTCGATAATGAATCGACCTGTATTGTCTGCAGCACTCTTCGGTTGCAACATTCGGCGCGTTATTTTTTAACGTCTTTGCCGGAAACGCTGTTTTTAGCACCGGTTAACCACAGCGTGGAATATAACTGGCTGAGGGAGGc                                      <  1:280737/151‑1 (MQ=255)                                                                                                    gAGTCATGTCGATAATGAATCGACCTGTATTGTCTGCAGCACTCTTCGGTTGCAACATTCGGCGCGTTATTTTTTAACGTCTTTGCCGGAAACGCTGTTTTTAGCACCGGTTAACCACAGCGTGGAATATAACTGGCTGAGGGAGGCGAt                                   <  2:46254/150‑1 (MQ=255)                                                                                                           gTCGATAATGAATCGACCTGTATTGTCTGCAGCACTCTTCGGTTGCAACATTCGGCGCGTTATTTTTTAACGTCTTTGCCGGAAACGCTGTTTTTAGCACCGGTTAACCACAGCGTGGAATATAACTGGCTGAGGGAGGCGATCCCGtttt                           >  2:133728/1‑151 (MQ=255)                                                                                                               aTAATGAATCGACCTGTATTGTCTGCAGCACTCTTCGGTTGCAACATTCGGCGCGTTATTTTTTAACGTCTTTGCCGGAAACGCTGTTTTTAGCACCGGTTAACCACAGCGTGGAATATAACTGGCTGAGGGAGGCGATCCCGTTTTTac                        >  2:306451/1‑150 (MQ=255)                                                                                                               aTAATGAATCGACCTGTATTGTCTGCAGCACTCTTCGGTTGCAACATTCGGCGCGTTATTTTTTAACGTCTTTGCCGGAAACGCTGTTTTTAGCACCGGTTAACCACAGCGTGGAATATAACTGGCTGAGGGAGGCGATCCCGTTTTTac                        >  2:104111/1‑150 (MQ=255)                                                                                                                        cGACCTGTATTGTCTGCAGCACTCTTCGGTTGCAACATTCGGCGCGTTATTTTTTAACGTCTTTGCCGGAAACGCTGTTTTTAGCACCGGTTAACCACAGCGTGGAATATAACTGGCTGAGGGAGGCGATCCCGTTTTTACAACAGGAATc              <  1:555977/151‑1 (MQ=255)                                                                                                                          aCCTGTATTGTCTGCAGCACTCTTCGGTTGCAACATTCGGCGCGTTATTTTTTAACGTCTTTGCCGGAAACGCTGTTTTTAGCACCGGTTAACCACAGCGTGGAATATAACTGGCTGAGGGAGGCGATCCCGTTTTTACAACAGGAATc              >  2:429675/1‑149 (MQ=255)                                                                                                                                aTTGTCTGCAGCACTCTTCGGTTGCAACATTCGGCGCGTTATTTTTTAACGTCTTTGCCGGAAACGCTGTTTTTAGCACCGGTTAACCACAGCGTGGAATATAACTGGCTGAGGGAGGCGATCCCGTTTTTACAACAGGAATCCAGATCg       <  1:90390/150‑1 (MQ=255)                                                                                                                                    tCTGCAGCACTCTTCGGTTGCAACATTCGGCGCGTTATTTTTTAACGTCTTTGCCGGAAACGCTGTTTTTAGCACCGGTTAACCACAGCGTGGAATATAACTGGCTGAGGGAGGCGATCCCGTTTTTACAACAGGAATCCAGATCGGCAAt  >  2:539361/1‑151 (MQ=255)                                                                                                                                         |                                                                                                                                                  AACGCAAGGAGCGGCGAAGCTGGAAATGCCGACGGGGGAGATTTTTACATTACGCCCGGGAAATGTGGTCCTGCTACCACAAAATTCAGCTCATCGCCTGAGTCATGTCGATAATGAATCGACCTGTATTGTCTGCGGCACTCTTCGGTTGCAACATTCGGCGCGTTATTTTTTAACGTCTTTGCCGGAAACGCTGTTTTTAGCACCGGTTAACCACAGCGTGGAATATAACTGGCTGAGGGAGGCGATCCCGTTTTTACAACAGGAATCCAGATCGGCAAT  >  NC_000913/320358‑320639

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑