Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	456,412	T→C	L427P (CTG→CCG)	tig →	peptidyl‑prolyl cis/trans isomerase (trigger factor)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	456,412	0	T	C	100.0%	161.7 / NA	44	L427P (CTG→CCG)	tig	peptidyl‑prolyl cis/trans isomerase (trigger factor)
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (30/14);  total (30/14)

AAGATCCGAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAATTTTGCATGGAACCGTGCGAAAAGCCTCTTTCGGTGTTAGCGTAACAACAAAA  >  NC_000913/456268‑456558                                                                                                                                                 |                                                                                                                                                   aaGATCCGAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCcgatga                                                                                                                                               >  2:463483/1‑150 (MQ=255) aaGATCCGAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCcgatga                                                                                                                                               >  2:145736/1‑150 (MQ=255)   gATCCGAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCCGATGAAcc                                                                                                                                            >  2:57147/1‑151 (MQ=255)                ttATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCCGATGAACCAGCAGGCGTAAtt                                                                                                                               >  1:266048/1‑151 (MQ=255)                ttATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCCGATGAACCAGCAGGCGTAAtt                                                                                                                               >  1:603056/1‑151 (MQ=255)                 tATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCCGATGAACCAGCAGGCGTAAttt                                                                                                                              >  2:63049/1‑151 (MQ=255)                 tATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCCGATGAACCAGCAGGCGTAAtt                                                                                                                               <  1:385517/150‑1 (MQ=255)                   tCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCCGATGAACCAGCAGGCGTAATTTAc                                                                                                                            >  2:340307/1‑151 (MQ=255)                   tCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCCGATGAACCAGCAGGCGTAATTTAc                                                                                                                            >  1:133762/1‑151 (MQ=255)                      aGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCCGATGAACCAGCAGGCGTAATTTACgca                                                                                                                         <  2:67509/151‑1 (MQ=255)                      aGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCCGATGAACCAGCAGGCGTAATTTACgc                                                                                                                          >  2:359822/1‑150 (MQ=255)                          cTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCCGATGAACCAGCAGGCGTAATTTACGCAGCAt                                                                                                                     >  2:595096/1‑151 (MQ=255)                               gCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCCGATGAACCAGCAGGCGTAATTTACGCAGCATAAcgc                                                                                                                >  1:119778/1‑151 (MQ=255)                                      cAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCCGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAAt                                                                                                         >  1:239667/1‑151 (MQ=255)                                         aGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCCGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCg                                                                                                      >  2:303948/1‑151 (MQ=255)                                           aaCTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCCGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGc                                                                                                     >  2:607666/1‑150 (MQ=255)                                               gATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCCGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACaaa                                                                                                <  2:239727/151‑1 (MQ=255)                                               gATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACGGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCCGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACaaa                                                                                                <  1:13133/151‑1 (MQ=255)                                                  ggACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCCGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGc                                                                                             <  2:578770/151‑1 (MQ=255)                                                  ggACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCCGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGc                                                                                             <  2:204908/151‑1 (MQ=255)                                                    acaacaTGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCCGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGccc                                                                                           >  1:435100/1‑151 (MQ=255)                                                      aacaTGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCCGATGAACCAGGAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAACGCCCGt                                                                                         >  2:312840/1‑151 (MQ=255)                                                               aaTGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCCGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGcc                                                                                  >  1:621665/1‑149 (MQ=255)                                                               aaTGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCCGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAg                                                                                <  1:74896/151‑1 (MQ=255)                                                                     gCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCCGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGgtggtg                                                                          >  2:362372/1‑151 (MQ=255)                                                                       tctGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCCGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTggg                                                                        >  1:605039/1‑151 (MQ=255)                                                                                    gCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCCGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTc                                                            >  1:44365/1‑150 (MQ=255)                                                                                          gAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCCGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAAtt                                                     >  2:157396/1‑151 (MQ=255)                                                                                           aaGGTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCCGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAAttt                                                    <  2:497340/151‑1 (MQ=255)                                                                                           aaGCTGTACTGGCGAAAGCGAAAGTGACGGAAAAAGAAACCACTTTCAACGAGCCGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAAttt                                                    <  1:9358/151‑1 (MQ=255)                                                                                            aGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCCGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAAtttt                                                   <  1:64478/151‑1 (MQ=255)                                                                                                   cTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCCGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAATTTTGCATgg                                             >  2:292604/1‑150 (MQ=255)                                                                                                     ggCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCCGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAATTTTGCATGGAAc                                          <  1:504805/151‑1 (MQ=255)                                                                                                     ggCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCCGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAATTTTGCATGGAAc                                          >  1:370090/1‑151 (MQ=255)                                                                                                                      cTGAAAAAGAAACCACTTTCAACGAGCCGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAATTTTGCATGGAACCGTGCGAAAAGCCTCtt                         >  1:129958/1‑151 (MQ=255)                                                                                                                        gAAAAAGAAACCACTTTCAACGAGCCGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAATTTTGCATGGAACCGTGCGAAAAGCCTCtt                         <  1:333890/149‑1 (MQ=255)                                                                                                                        gAAAAAGAAACCACTTTCAACGAGCCGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAATTTTGCATGGAACCGTGCGAAAAGCCTCTTTc                       >  1:495176/1‑151 (MQ=255)                                                                                                                         aaaaaGAAACCACTTTCAACGAGCCGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAATTTTGCATGGAACCGTGCGAAAAGCCTCTTTCg                      >  1:470332/1‑151 (MQ=255)                                                                                                                            aaGAAACCACTTTCAACGAGCCGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAATTTTGCATGGAACCGTGCGAAAAGCCTCTTTCGgtg                   >  2:248520/1‑151 (MQ=255)                                                                                                                                 aCCACTTTCAACGAGCCGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAATTTTGCATGGAACCGTGCGAAAAGCCTCTTTCGGTGTTAGc              >  2:351003/1‑151 (MQ=255)                                                                                                                                 aCCACTTTCAACGAGCCGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAATTTTGCATGGAACCGTGCGAAAAGCCTCTTTCGGTGTTAGc              >  1:371831/1‑151 (MQ=255)                                                                                                                                  ccACTTTCAACGAGCCGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAATTTTGCATGGAACCGTGCGAAAAGCCTCTTTCGGTGTTAGCg             >  2:88796/1‑151 (MQ=255)                                                                                                                                     cTTTCAACGAGCCGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAATTTTGCATGGAACCGTGCGAAAAGCCTCTTTCGGTGTTAGCGTa           >  2:174763/1‑150 (MQ=255)                                                                                                                                       ttCAACGAGCCGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAATTTTGCATGGAACCGTGCGAAAAGCCTCTTTCGGTGTTAGCGTaac         >  2:326444/1‑150 (MQ=255)                                                                                                                                           aCGAGCCGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAATTTTGCATGGAACCGTGCGAAAAGCCTCTTTCGGTGTTAGCGTAAcaacaa    <  1:262286/151‑1 (MQ=255)                                                                                                                                             gAGCCGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAATTTTGCATGGAACCGTGCGAAAAGCCTCTTTCGGTGTTAGCGTAACAACaaaa  <  1:239596/151‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                   AAGATCCGAAAGAAGTTATCGAGTTCTACAGCAAAAACAAAGAACTGATGGACAACATGCGCAATGTTGCTCTGGAAGAACAGGCTGTTGAAGCTGTACTGGCGAAAGCGAAAGTGACTGAAAAAGAAACCACTTTCAACGAGCTGATGAACCAGCAGGCGTAATTTACGCAGCATAACGCGCTAAATTCGCACAAAGGCCCGTCACCGCCAGGTGGTGGGCTTTTTTTTGTCATGAATTTTGCATGGAACCGTGCGAAAAGCCTCTTTCGGTGTTAGCGTAACAACAAAA  >  NC_000913/456268‑456558

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑