Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 515,571 A→G I68T (ATC→ACC)  qmcA ← PHB domain membrane‑anchored putative protease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913515,5710AG95.7% 146.8 / ‑1.3 46I68T (ATC→ACC) qmcAPHB domain membrane‑anchored putative protease
Reads supporting (aligned to +/- strand):  ref base A (2/0);  new base G (25/19);  total (27/19)
Fisher's exact test for biased strand distribution p-value = 5.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.52e-01

GGATGTTAGTCATGGTCAGGTTGATGATCGCCAGCTCCAGATTGCTGACTTCATAAGCCGCGCGCGGCGCGTCAATCACCTGAATAAAGCACACGGCGTCGATGGTAACGTTGGCGTTATCTTTCGAGATAACTTCCTGGGAAGGGATATCGAGCACTTGCTCCATCATATTGATCTTGCGACCAATGCGATCCATAAACGGCACCACCAGACTGAGCCCCGGCTGTAACGTTTTGGTATAGCGACCAAAGCGTTCTACTGTCCACTGATAGCCCTGCGGTACGATTTTGAC  >  NC_000913/515425‑515716
                                                                                                                                                  |                                                                                                                                                 
ggATGTTAGTCATGGTCAGGTTGATGATCGCCAGCTCCAGATTGCTGACTTCATAAGCCGCGCGCGGCGCGTCAATCACCTGAATAAAGCACACGGCGTCGATGGTAACGTTGGCGTTATCTTTCGAGATAACTTCCTGGGAAGGGGTATc                                                                                                                                               <  2:261331/151‑1 (MQ=255)
   tGTTAGTCATGGTCAGGTTGATGATCGCCAGCTCCAGATTGCTGACTTCAT‑AGCCGCGCGCGGCGCGTCAATCACCTGAATAAAGCACACGGCGTCGATGGTAACGTTGGCGTTATCTTTCGAGATAACTTCCTGGGAAGGGGTATCGAGc                                                                                                                                           >  1:626328/1‑151 (MQ=255)
       aGTCATGGTCAGGTTGATGATCGCCAGCTCCAGATTGCTGACTTCATAAGCCGCGCGCGGCGCGTCAATCACCTGAATAAAGCACACGGCGTCGATGGTAACGTTGGCGTTATCTTTCGAGATAACTTCCTGGGAAGGGGTATCGAGCACt                                                                                                                                        <  2:496383/151‑1 (MQ=255)
             ggTCAGGTTGATGATCGCCAGCTCCAGATTGCTGACTTCATAAGCCGCGCGCGGCGCGTCAATCACCTGAATAAAGCACACGGCGTCGATGGTAACGTTGGCGTTATCTTTCGAGATAACTTCCTGGGAAGGGGTATCGAGCACTTGCTcc                                                                                                                                  <  1:593649/151‑1 (MQ=255)
               tCAGGTTGATGATCGCCAGCTCCAGATTGCTGACTTCATAAGCCGCGCGCGGCGCGTCAATCACCTGAATAAAGCACACGGCGTCGATGGTAACGTTGGCGTTATCTTTCGAGATAACTTCCTGGGAAGGGGTATCGAGCACTTGCTCcat                                                                                                                                >  1:33342/1‑151 (MQ=255)
                    ttGATGATCGCCAGCTCCAGATTGCTGACTTCATAAGCCGCGCGCGGCGCGTCAATCACCTGAATAAAGCACACGGCGTCGATGGTAACGTTGGCGTTATCTTTCGAGATAACTTCCTGGGAAGGGGTATCGAGCACTTGCTCCATCatat                                                                                                                           >  1:307183/1‑151 (MQ=255)
                     tgatgaTCGCCAGCTCCAGATTGCTGACTTCATAAGCCGCGCGCGGCGCGTCAATCACCTGAATAAAGCACACGGCGTCGATGGTAACGTTGGCGTTATCTTTCGAGATAACTTCCTGGGAAGGGGTATCGAGCACTTGCTCCATCatat                                                                                                                           <  2:107335/150‑1 (MQ=255)
                     tgatgaTCGCCAGCTCCAGATTGCTGACTTCATAAGCCGCGCGCGGCGCGTCAATCACCTGAATAAAGCACACGGCGTCGATGGTAACGTTGGCGTTATCTTTCGAGATAACTTCCTGGGAAGGGGTATCGAGCACTTGCTCCATCatat                                                                                                                           <  2:2886/150‑1 (MQ=255)
                       atgatCGCCAGCTCCAGATTGCTGACTTCATAAGCCGCGCGCGGCGCGTCAATCACCTGAATAAAGCACACGGCGTCGATGGTAACGTTGGCGTTATCTTTCGAGATAACTTCCTGGGAAGGGGTATCGAGCACTTGCTCCATCATATTGa                                                                                                                        <  1:185813/151‑1 (MQ=255)
                                 gCTCCAGATTGCTGACTTCATAAGCCGCGCGCGGCGCGTCAATCACCTGAATAAAGCACACGGCGTCGATGGTAACGTTGGCGTTATCTTTCGAGATAACTTCCTGGGAAGGGGTATCGAGCACTTGCTCCATCATATTGATCTTGCGAcc                                                                                                              <  1:577659/151‑1 (MQ=255)
                                    ccAGATTGCTGACTTCATAAGCCGCGCGCGGCGCGTCAATCACCTGAATAAAGCACACGGCGTCGATGGTAACGTTGGCGTTATCTTTCGAGATAACTTCCTGGGAAGGGGTATCGAGCACTTGCTCCATCATATTGATCTTGCGACCa                                                                                                             <  2:455052/149‑1 (MQ=255)
                                             tGACTTCATAAGCCGCGCGCGGCGCGTCAATCACCTGAATAAAGCACACGGCGTCGATGGTAACGTTGGCGTTATCTTTCGAGATAACTTCCTGGGAAGGGGTATCGAGCACTTGCTCCATCATATTGATCTTGCGACCAATGCGATCCAt                                                                                                  >  2:454713/1‑151 (MQ=255)
                                              gACTTCATAAGCCGCGCGCGGCGCGTCAATCACCTGAATAAAGCACACGGCGTCGATGGTAACGTTGGCGTTATCTTTCGAGATAACTTCCTGGGAAGGGGTATCGAGCACTTGCTCCATCATATTGATCTTGCGACCAATGCGATCCATa                                                                                                 <  1:547559/151‑1 (MQ=255)
                                                      aaGCCGCGCGCGGCGCGTCAATCACCTGAATAAAGCACACGGCGTCGATGGTAACGTTGGCGTTATCTTTCGAGATAACTTCCTGGGAAGGGGTATCGAGCACTTGCTCCATCATATTGATCTTGCGACCAATGCGATCCATAAACGGcac                                                                                         >  2:330637/1‑151 (MQ=255)
                                                       aGCCGCGCGCGGCGCGTCAATCACCTGAATAAAGCACACGGCGTCGATGGTAACGTTGGCGTTATCTTTCGAGATAACTTCCTGGGAAGGGGTATCGAGCACTTGCTCCATCATATTGATCTTGCGACCAATGCGATCCATAAACGGcacc                                                                                        >  2:415955/1‑151 (MQ=255)
                                                       aGCCGCGCGCGGCGCGTCAATCACCTGAATAAAGCACACGGCGTCGATGGTAACGTTGGCGTTATCTTTCGAGATAACTTCCTGGGAAGGGGTATCGAGCACTTGCTCCATCATATTGATCTTGCGACCAATGCGATCCATAAACGGcacc                                                                                        >  2:524524/1‑151 (MQ=255)
                                                             gcgcggcgcgTCAATCACCTGAATAAAGCACACGGCGTCGATGGTAACGTTGGCGTTATCTTTCGAGATAACTTCCTGGGAAGGGGTATCGAGCACTTGCTCCATCATATTGATCTTGCGACCAATGCGATCCATAAACGGCACCACCAGa                                                                                  <  2:460090/151‑1 (MQ=255)
                                                               gcggcgcgTCAATCACCTGAATAAAGCACACGGCGTCGATGGTAACGTTGGCGTTATCTTTCGAGATAACTTCCTGGGAAGGGGTATCGAGCACTTGCTCCATCATATTGATCTTGCGACCAATGCGATCCATAAACGGCACCACCAGACt                                                                                <  2:479828/151‑1 (MQ=255)
                                                                       tCAATCACCTGAATAAAGCACACGGCGTCGATGGTAACGTTGGCGTTATCTTTCGAGATAACTTCCTGGGAAGGGGTATCGAGCACTTGCTCCATCATATTGATCTTGCGACCAATGCGATCCATAAACGGCACCACCAGACTGAGCCCCg                                                                        >  1:582820/1‑151 (MQ=255)
                                                                            cACCTGAATAAAGCACACGGCGTCGATGGTAACGTTGGCGTTATCTTTCGAGATAACTTCCTGGGAAGGGGTATCGAGCACTTGCTCCATCATATTGATCTTGCGACCAATGCGATCCATAAACGGCACCACCAGACTGAGCCCCGGCTGt                                                                   >  2:580680/1‑151 (MQ=255)
                                                                             aCCTGAATAAAGCACACGGCGTCGATGGTAACGTTGGCGTTATCTTTCGAGATAACTTCCTGGGAAGGGGTATCGAGCACTTGCTCCATCATATTGATCTTGCGACCAATGCGATCCATAAACGGCACCACCAGACTGAGCCCCGGCTGt                                                                   >  2:560043/1‑150 (MQ=255)
                                                                                 gAATAAAGCACACGGCGTCGATGGTAACGTTGGCGTTATCTTTCGAGATAACTTCCTGGGAAGGGGTATCGAGCACTTGCTCCATCATATTGATCTTGCGACCAATGCGATCCATAAACGGCACCACCAGACTGAGCCCCGGCTGTAACGt                                                              <  1:142720/151‑1 (MQ=255)
                                                                                        gCACACGGCGTCGATGGTAACGTTGGCGTTATCTTTCGAGATAACTTCCTGGGAAGGGGTATCGAGCACTTGCTCCATCATATTGATCTTGCGACCAATGCGATCCATAAACGGCACCACCAGACTGAGCCCCGGCTGTAACGTTTTGGta                                                       <  2:105420/151‑1 (MQ=255)
                                                                                           cacGGCGTCGATGGTAACGTTGGCGTTATCTTTCGAGATAACTTCCTGGGAAGGGGTATCGAGCACTTGCTCCATCATATTGATCTTGCGACCAATGCGATCCATAAACGGCACCACCAGACTGAGCCCCGGCTGTAACGTTTTGGtat                                                      >  2:150065/1‑149 (MQ=255)
                                                                                           cacGGCGTCGATGGTAACGTTGGCGTTATCTTTCGAGATAACTTCCTGGGAAGGGGTATCGAGCACTTGCTCCATCATATTGATCTTGCGACCAATGCGATCCATAAACGGCACCACCAGACTGAGCCCCGGCTGTAACGTTTTGGTATAg                                                    <  2:507860/151‑1 (MQ=255)
                                                                                              ggCGTCGATGGTAACGTTGGCGTTATCTTTCGAGATAACTTCCTGGGAAGGGGTATCGAGCACTTGCTCCATCATATTGATCTTGCGACCAATGCGATCCATAAACGGCACCACCAGACTGAGCCCCGGCTGTAACGTTTTGGTATAg                                                    >  2:564318/1‑148 (MQ=255)
                                                                                                 gTCGATGGTAACGTTGGCGTTATCTTTCGAGATAACTTCCTGGGAAGGTGTATCGAGCACTTGCTCCATCATATTGATCTTGCGACCAATGCGATCCATAAACGGCACCACCAGACTGAGCCCCGGCTGTAACGTTTTGGTATAGCGAcc                                               >  1:518165/1‑150 (MQ=255)
                                                                                                  tCGATGGTAACGTTGGCGTTATCTTTCGAGATAACTTCCTGGGAAGGGGTATCGAGCACTTGCTCCATCATATTGATCTTGCGACCAATGCGATCCATAAACGGCACCACCAGACTGAGCCCCGGCTGTAACGTTTTGGTATAGCGACCa                                              >  2:537357/1‑150 (MQ=255)
                                                                                                   cGATGGTAACGTTGGCGTTATCTTTCGAGATAACTTCCTGGGAAGGGGTATCGAGCACTTGCTCCATCATATTGATCTTGCGACCAATGCGATCCATAAACGGCACCACCAGACTGAGCCCCGGCTGTAACGTTTTGGTATAGCGACCaa                                             >  2:193592/1‑150 (MQ=255)
                                                                                                    gATGGTAACGTTGGCGTTATCTTTCGAGATAACTTCCTGGGAAGGGATATCGAGCACTTGCTCCATCATATTGATCTTGCGACCAATGCGATCCATAAACGGCACCACCAGACTGAGCCCCGGCTGTAACGTTTTGGAATAGCGACCaa                                             >  1:242448/1‑149 (MQ=255)
                                                                                                     aTGGTAACGTTGGCGTTATCTTTCGAGATAACTTCCTGGGAAGGGGTATCGAGCACTTGCTCCATCATATTGATCTTGCGACCAATGCGATCCATAAACGGCACCACCAGACTGAGCCCCGGCTGTAACGTTTTGGTATAGCGACCAAAGc                                          >  1:479806/1‑151 (MQ=255)
                                                                                                         tAACGTTGGCGTTATCTTTCGAGATAACTTCCTGGGAAGGGGTATCGAGCACTTGCTCCATCATATTGATCTTGCGACCAATGCGATCCATAAACGGCACCACCAGACTGAGCCCCGGCTGTAACGTTTTGGTATAGCGACCAAAGCGTTc                                      >  2:243517/1‑151 (MQ=255)
                                                                                                          aaCGTTGGCGTTATCTTTCGAGATAACTTCCTGGGAAGGGGTATCGAGCACTTGCTCCATCATATTGATCTTGCGACCAATGCGATCCATAAACGGCACCACCAGACTGAGCCCCGGCTGTAACGTTTTGGTATAGCGACCAAAGCGTTCt                                     >  1:34661/1‑151 (MQ=255)
                                                                                                            cGTTGGCGTTATCTTTCGAGATAACTTCCTGGGAAGGGGTATCGAGCACTTGCTCCATCATATTGATCTTGCGACCAATGCGATCCATAAACGGCACCACCAGACTGAGCCCCGGCTGTAACGTTTTGGTATAGCGACCAAAGCGTTCTa                                    <  2:487192/150‑1 (MQ=255)
                                                                                                            cGTTGGCGTTATCTTTCGAGATAACTTCCTGGGAAGGGGTATCGAGCACTTGCTCCATCATATTGATCTTGCGACCAATGCGATCCATAAACGGCACCACCAGACTGAGCCCCGGCTGTAACGTTTTGGTATAGCGACCAAAGCGTTCTAc                                   <  1:560055/151‑1 (MQ=255)
                                                                                                              ttGGCGTTATCTTTCGAGATAACTTCCTGGGAAGGGGTATCGAGCACTTGCTCCATCATATTGATCTTGCGACCAATGCGATCCATAAACGGCACCACCAGACTGAGCCCCGGCTGTAACGTTTTGGTATAGCGACCAAAGCGTTCTACTg                                 >  2:8810/1‑151 (MQ=255)
                                                                                                                 gCGTTATCTTTCGAGATAACTTCCTGGGAAGGGGTATCGAGCACTTGCTCCATCATATTGATCTTGCGACCAATGCGTTCCATAAACGGCACCACCAGACTGAGCCCCGGCTGTAACGTTTTGGTATAGCGACCAAAGCGTTCTACTGTcc                              <  2:300935/151‑1 (MQ=255)
                                                                                                                 gCGTTATCTTTCGAGATAACTTCCTGGGAAGGGGTATCGAGCACTTGCTCCATCATATTGATCTTGCGACCAATGCGATCCATAAACGGCACCACCAGACTGAGCCCCGGCTGTAACGTTTTGGTATAGCGACCAAAGCGTTCTACTGTcc                              >  1:218374/1‑151 (MQ=255)
                                                                                                                  cGTTATCTTTCGAGATAACTTCCTGGGAAGGGATATCGAGCACTTGCTCCATCATATTGATCTTGCGACCAATGCGATCCATAAACGGCACCACCAGACTGAGCCCCGGCTGTAACGTTTTGGTATAGCGACCAAAGCGTTCTACTGTcc                              >  1:405689/1‑150 (MQ=255)
                                                                                                                    ttATCTTTCGAGATAACTTCCTGGGAAGGGGTATCGAGCACTTGCTCCATCATATTGATCTTGCGACCAATGCGATCCATAAACGGCACCACCAGACTGAGCCCCGGCTGTAACGTTTTGGTATAGCGACCAAAGCGTTCTACTGTCCACt                           >  1:588735/1‑151 (MQ=255)
                                                                                                                                aTAACTTCCTGGGAAGGGGTATCGAGCACTTGCTCCATCATATTGATCTTGCGACCAATGCGATCCATAAACGGCACCACCAGACTGAGCCCCGGCTGTAACGTTTTGGTATAGCGACCAAAGCGTTCTACTGTCCACTGATAGCCCTGCg               <  1:132745/151‑1 (MQ=255)
                                                                                                                                  aaCTTCCTGGGAAGGGGTATCGAGCACTTGCTCCATCATATTGATCTTGCGACCAATGCGATCCATAAACGGCACCACCAGACTGAGCCCCGGCTGTAACGTTTTGGTATAGCGACCAAAGCGTTCTACTGTCCACTGATAGCCCTGCGGt             >  1:352131/1‑151 (MQ=255)
                                                                                                                                      tCCTGGGAAGGGGTATCGAGCACTTGCTCCATCATATTGATCTTGCGACCAATGCGATCCATAAACGGCACCACCAGACTGAGCCCCGGCTGTAACGTTTTGGTATAGCGACCAAAGCGTTCTACTGTCCACTGATAGCCCTGCGGTACg          <  2:550277/150‑1 (MQ=255)
                                                                                                                                         tGGGAAGGGGTATCGAGCACTTGCTCCATCATATTGATCTTGCGACCAATGCGATCCATAAACGGCACCACCAGACTGAGCCCCGGCTGTAACGTTTTGGTATAGCGACCAAAGCGTTCTACTGTCCACTGATAGCCCTGCGGTACGAttt      >  2:157322/1‑151 (MQ=255)
                                                                                                                                            gAAGGGGTATCGAGCACTTGCTCCATCATATTGATCTTGCGACCAATGCGATCCATAAACGGCACCACCAGACTGAGCCCCGGCTGTAACGTTTTGGTATAGCGACCAAAGCGTTCTACTGTCCACTGATAGCCCTGCGGTACGATTTTg    >  1:258820/1‑150 (MQ=255)
                                                                                                                                             aaGGGGTATCGAGCACTTGCTCCATCATATTGATCTTGCGACCAATGCGATCCATAAACGGCACCACCAGACTGAGCCCCGGCTGTAACGTTTTGGTATAGCGACCAAAGCGTTCTACTGTCCACTGATAGCCCTGCGGTACGATTTTGac  >  1:228442/1‑151 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
GGATGTTAGTCATGGTCAGGTTGATGATCGCCAGCTCCAGATTGCTGACTTCATAAGCCGCGCGCGGCGCGTCAATCACCTGAATAAAGCACACGGCGTCGATGGTAACGTTGGCGTTATCTTTCGAGATAACTTCCTGGGAAGGGATATCGAGCACTTGCTCCATCATATTGATCTTGCGACCAATGCGATCCATAAACGGCACCACCAGACTGAGCCCCGGCTGTAACGTTTTGGTATAGCGACCAAAGCGTTCTACTGTCCACTGATAGCCCTGCGGTACGATTTTGAC  >  NC_000913/515425‑515716

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑