Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 577,085 G→A intergenic (‑260/‑313) nmpC ← / → essD DLP12 prophage; truncated outer membrane porin (pseudogene);IS, phage, Tn; Phage or Prophage Related; outer membrane porin protein; locus of qsr prophage/DLP12 prophage; putative phage lysis protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913577,0850GA100.0% 188.9 / NA 48intergenic (‑260/‑313)nmpC/essDDLP12 prophage; truncated outer membrane porin (pseudogene);IS, phage, Tn; Phage or Prophage Related; outer membrane porin protein; locus of qsr prophage/DLP12 prophage; putative phage lysis protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/23);  total (25/23)

AAGTAGATCTCTATTTTTATCTGAACTTTTTTCTATCGAATCCTATTCATGGCTCTTGGCTGAATAAAAATAAATCTATTAGCCAATTTATATTAACGGCTGTTATTTATAAGTGCTCTATAATTTGAAGGTTCAATTTAAACCGGCTAAAAATAACACTGGAAATTATTTTTTGGTTATTTGTTGAGATTTGCTTATGTATTTGTAGTGGTGTTTTCAATACTCGGTAGCATTCTCTCAAATATCATTTAGTGGTTTACGTACGTAAAAAATTGGTTATGCTGTT  >  NC_000913/576940‑577225
                                                                                                                                                 |                                                                                                                                            
aaGTAGATCTCTATTTTTATCTGAACTTTTTTCTATCGAATCCTATTCATGGCTCTTGGCTGAATAAAAATAAATCTATTAGCCAATTTATATTAACGGCTGTTATTTATAAGTGCTCTATAATTTGAAGGTTCAATTTAAACCGACTaaa                                                                                                                                         <  2:544959/151‑1 (MQ=255)
    aGATCTCTATTTTTATCTGAACTTTTTTCTATCGAATCCTATTCATGGCTCTTGGCTGAATAAAAATAAATCTATTAGCCAATTTATATTAACGGCTGTTATTTATAAGTGCTCTATAATTTGAAGGTTCAATTTAAACCGACTAAAAAt                                                                                                                                      <  1:80394/150‑1 (MQ=255)
        ctctATTTTTATCTGAACTTTTTTCTATCGAATCCTATTCATGGCTCTTGGCTGAATAAAAATAAATCTATTAGCCAATTTATATTAACGGCTGTTATTTATAAGTGCTCTATAATTTGAAGGTTCAATTTAAACCGACTAAAAATAacac                                                                                                                                 >  1:169632/1‑151 (MQ=255)
          ctATTTTTATCTGAACTTTTTTCTATCGAATCCTATTCATGGCTCTTGGCTGAATAAAAATAAATCTATTAGCCAATTTATATTAACGGCTGTTATTTATAAGTGCTCTATAATTTGAAGGTTCAATTTAAACCGACTAAAAATaa                                                                                                                                    <  1:308927/146‑1 (MQ=255)
            aTTTTTATCTGAACTTTTTTCTATCGAATCCTATTCATGGCTCTTGGCTGAATAAAAATAAATCTATTAGCCAATTTATATTAACGGCTGTTATTTATAAGTGCTCTATAATTTGAAGGTTCAATTTAAACCGACTAAAAATAACACt                                                                                                                                <  1:155820/148‑1 (MQ=255)
            aTTTTTATCTGAACTTTTTTCTATCGAATCCTATTCATGGCTCTTGGCTGAATAAAAATAAATCTATTAGCCAATTTATATTAACGGCTGTTATTTATAAGTGCTCTATAATTTGAAGGTTCAATTTAAACCGACTAAAAATAACACTgg                                                                                                                              >  1:275132/1‑150 (MQ=255)
              ttttATCTGAACTTTTTTCTATCGAATCCTATTCATGGCTCTTGGCTGAATAAAAATAAATCTATTAGCCAATTTATATTAACGGCTGTTATTTATAAGTGCTCTATAATTTGAAGGTTCAATTTAAACCGACTAAAAATAACACTGGaa                                                                                                                            >  2:228927/1‑150 (MQ=255)
                       aaCTTTTTTCTATCGAATCCTATTCATGGCTCTTGGCTGAATAAAAATAAATCTATTAGCCAATTTATATTAACGGCTGTTATTTATAAGTGCTCTATAATTTGAAGGTTCAATTTAAACCGACTAAAAATAACACTGGAAATTAtttttt                                                                                                                  >  1:225371/1‑151 (MQ=255)
                           tttttCTATCGAATCCTATTCATGGCTCTTGGCTGAATAAAAATAAATCTATTAGCCAATTTATATTAACGGCTGTTATTTATAAGTGCTCTATAATTTGAAGGTTCAATTTAAACCGACTAAAAATAACACTGGAAATTATTTTTTgg                                                                                                                <  1:300785/149‑1 (MQ=255)
                           tttttCTATCGAATCCTATTCATGGCTCTTGGCTGAATAAAAATAAATCTATTAGCCAATTTATATTAACGGCTGTTATTTATAAGTGCTCTATAATTTGAAGGTTCAATTTAAACCGACTAAAAATAACACTGGAAATTATTTTTTGGt                                                                                                               <  1:92857/150‑1 (MQ=255)
                                    cGAATCCTATTCATGGCTCTTGGCTGAATAAAAATAAATCTATTAGCCAATTTATATTAACGGCTGTTATTTATAAGTGCTCTATAATTTGAAGGTTCAATTTAAACCGACTAAAAATAACACTGGAAATTATTTTTTGGTTATttgttg                                                                                                      >  1:542975/1‑150 (MQ=255)
                                         ccTATTCATGGCTCTTGGCTGAATAAAAATAAATCTATTAGCCAATTTATATTAACGGCTGTTATTTATAAGTGCTCTATAATTTGAAGGTTCAATTTAAACCGACTAAAAATAACACTGGAAATTATTTTTTGGTTATTTGTTGAGAttt                                                                                                <  1:288463/151‑1 (MQ=255)
                                         ccTATTCATGGCTCTTGGCTGAATAAAAATAAATCTATTAGCCAATTTATATTAACGGCTGTTATTTATAAGTGCTCTATAATTTGAAGGTTCAATTTAAACCGACTAAAAATAACACTGGAAATTATTTTTTGGTTATTTGTTGAGAttt                                                                                                >  2:417526/1‑151 (MQ=255)
                                           tATTCATGGCTCTTGGCTGAATAAAAATAAATCTATTAGCCAATTTATATTAACGGCTGTTATTTATAAGTGCTCTATAATTTGAAGGTTCAATTTAAACCGACTAAAAATAACACTGGAAATTATTTTTTGGTTATTTGTTga                                                                                                     <  1:192186/144‑1 (MQ=255)
                                           tATTCATGGCTCTTGGCTGAATAAAAATAAATCTATTAGCCAATTTATATTAACGGCTGTTATTTATAAGTGCTCTATAATTTGAAGGTTCAATTTAAACCGACTAAAAATAACACTGGAAATTATTTTTTGGTTATTTGTTga                                                                                                     >  2:192181/1‑144 (MQ=255)
                                               cATGGCTCTTGGCTGAATAAAAATAAATCTATTAGCCAATTTATATTAACGGCTGTTATTTATAAGTGCTCTATAATTTGAAGGTTCAATTTAAACCGACTAAAAATAACACTGGAAATTATTTTTTGGTTATTTGTTGAGATTTGCTTAt                                                                                          <  2:282312/151‑1 (MQ=255)
                                                      ctTGGCTGAATAAAAATAAATCTATTAGCCAATTTATATTAACGGCTGTTATTTATAAGTGCTCTATAATTTGAAGGTTCAATTTAAACCGACTAAAAATAACACTGGAAATTATTTTTTGGTTATTTGTTGAGATTTGCTTATGTAtt                                                                                     <  1:91905/149‑1 (MQ=255)
                                                        tGGCTGAATAAAAATAAATCTATTAGCCAATTTATATTAACGGCTGTTATTTATAAGTGCTCTATAATTTGAAGGTTCAATTTAAACCGACTAAAAATAACACTGGAAATTATTTTTTGGTTATTTGTTGAGATTTGCTTATGTATTTGt                                                                                  <  1:369089/150‑1 (MQ=255)
                                                         ggCTGAATAAAAATAAATCTATTAGCCAATTTATATTAACGGCTGTTATTTATAAGTGCTCTATAATTTGAAGGTTCAATTTAAACCGACTAAAAATAACACTGGAAATTATTTTTTGGTTATTTGTTGAGATTTGCTTATGTATTTGTAg                                                                                <  2:588004/151‑1 (MQ=255)
                                                          gCTGAATAAAAATAAATCTATTAGCCAATTTATATTAACGGCTGTTATTTATAAGTGCTCTATAATTTGAAGGTTCAATTTAAACCGACTAAAAATAACACTGGAAATTATTTTTTGGTTATTTGTTGAGATTTGCTTATGTATTTGTAgt                                                                               >  2:105696/1‑151 (MQ=255)
                                                              aataaaaataaaTCTATTAGCCAATTTATATTAACGGCTGTTATTTATAAGTGCTCTATAATTTGAAGGTTCAATTTAAACCGACTAAAAATAACACTGGAAATTATTTTTTGGTTATTTGTTGAGATTTGCTTATGTATTTGTAgtggtg                                                                           >  2:602308/1‑151 (MQ=255)
                                                              aataaaaataaaTCTATTAGCCAATTTATATTAACGGCTGTTATTTATAAGTGCTCTATAATTTGAAGGTTCAATTTAAACCGACTAAAAATAACACTGGAAATTATTTTTTGGTTATTTGTTGAGATTTGCTTATGTATTTGTAgtggtg                                                                           >  2:184794/1‑151 (MQ=255)
                                                                  aaaataaaTCTATTAGCCAATTTATATTAACGGCTGTTATTTATAAGTGCTCTATAATTTGAAGGTTCAATTTAAACCGACTAAAAATAACACTGGAAATTATTTTTTGGTTATTTGTTGAGATTTGCTTATGTATTTGTAGTGGTGtttt                                                                       >  2:94816/1‑151 (MQ=255)
                                                                           cTATTAGCCAATTTATATTAACGGCTGTTATTTATAAGTGCTCTATAATTTGAAGGTTCAATTTAAACCGACTAAAAATAACACTGGAAATTATTTTTTGGTTATTTGTTGAGATTTGCTTATGTATTTGTAGTGGTGTTTTCAATACTCg                                                              >  1:568660/1‑151 (MQ=255)
                                                                           cTATTAGCCAATTTATATTAACGGCTGTTATTTATAAGTGCTCTATAATTTGAAGGTTCAATTTAAACCGACTAAAAATAACACTGGAAATTATTTTTTGGTTATTTGTTGAGATTTGCTTATGTATTTGTAGTGGTGTTTTCAATACTCg                                                              >  1:486036/1‑151 (MQ=255)
                                                                            tATTAGCCAATTTATATTAACGGCTGTTATTTATAAGTGCTCTATAATTTGAAGGTTCAATTTAAACCGACTAAAAATAACACTGGAAATTATTTTTTGGTTATTTGTTGAGATTTGCTTATGTATTTGTAGTGGTGTTTTCAATACTCgg                                                             >  1:211687/1‑151 (MQ=255)
                                                                                  ccAATTTATATTAACGGCTGTTATTTATAAGTGCTCTATAATTTGAAGGTTCAATTTAAACCGACTAAAAATAACACTGGAAATTATTTTTTGGTTATTTGTTGAGATTTGCTTATGTATTTGTAGTGGTGTTTTCAATACTCGGTAGCAt                                                       >  2:190516/1‑151 (MQ=255)
                                                                                         atatTAACGGCTGTTATTTATAAGTGCTCTATAATTTGAAGGTTCAATTTAAACCGACTAAAAATAACACTGGAAATTATTTTTTGGTTATTTGTTGAGATTTGCTTATGTATTTGTAGTGGTGTTTTCAATACTCGGTAGCATTCTCTCa                                                <  1:470127/151‑1 (MQ=255)
                                                                                          tatTAACGGCTGTTATTTATAAGTGCTCTATAATTTGAAGGTTCAATTTAAACCGACTAAAAATAACACTGGAAATTATTTTTTGGTTATTTGTTGAGATTTGCTTATGTATTTGTAGTGGTGTTTTCAATACTCGGTAGCATTCTCTCa                                                >  1:481805/1‑150 (MQ=255)
                                                                                             tAACGGCTGTTATTTATAAGTGCTCTATAATTTGAAGGTTCAATTTAAACCGACTAAAAATAACACTGGAAATTATTTTTTGGTTATTTGTTGAGATTTGCTTATGTATTTGTAGTGGTGTTTTCAATACTCGGTAGCATTCTCTCAAat                                             <  1:325218/150‑1 (MQ=255)
                                                                                                cGGCTGTTATTTATAAGTGCTCTATAATTTGAAGGTTCAATTTAAACCGACTAAAAATAACACTGGAAATTATTTTTTGGTTATTTGTTGAGATTTGCTTATGTATTTGTAGTGGTGTTTTCAATACTCGGTAGCATTCTCTCAAATATCa                                         <  1:503270/151‑1 (MQ=255)
                                                                                                   cTGTTATTTATAAGCGCTCTATAATTTGAAGGTTCAATTTAAACCGACTAAAAATAACACTGGAAATTATTTTTTGGTTATTTGTTGAGATTTGCTTATGTATTTGTAGTGGTGTTTTCAATACTCGGTAGCATTCTCTCAAATATCAttt                                      >  2:314219/1‑151 (MQ=255)
                                                                                                     gTTATTTATAAGTGCTCTATAATTTGAAGGTTCAATTTAAACCGACTAAAAATAACACTGGAAATTATTTTTTGGTTATTTGTTGAGATTTGCTTATGTATTTGTAGTGGTGTTTTCAATACTCGGTAGCATTCTCTCAAATATCATTTa                                     <  2:212854/150‑1 (MQ=255)
                                                                                                     gTTATTTATAAGTGCTCTATAATTTGAAGGTTCAATTTAAACCGACTAAAAATAACACTGGAAATTATTTTTTGGTTATTTGTTGAGATTTGCTTATGTATTTGTAGTGGTGTTTTCAATACTCGGTAGCATTCTCTCAAATATCATTTAg                                    <  1:417533/151‑1 (MQ=255)
                                                                                                      ttatttatAAGTGCTCTATAATTTGAAGGTTCAATTTAAACCGACTAAAAATAACACTGGAAATTATTTTTTGGTTATTTGTTGAGATTTGCTTATGTATTTGTAGTGGTGTTTTCAATACTCGGTAGCATTCTCTCAAATATCATTTAGt                                   >  1:225873/1‑151 (MQ=255)
                                                                                                        atttatAAGTGCTCTATAATTTGAAGGTTCAATTTAAACCGACTAAAAATAACACTGGAAATTATTTTTTGGTTATTTGTTGAGATTTGCTTATGTATTTGTAGTGGTGTTTTCAATACTCGGTAGCATTCTCTCAAATATCATTTAGTgg                                 >  2:527929/1‑151 (MQ=255)
                                                                                                        atttatAAGTGCTCTATAATTTGAAGGTTCAATTTAAACCGACTAAAAATAACACTGGAAATTATTTTTTGGTTATTTGTTGAGATTTGCTTATGTATTTGTAGTGGTGTTTTCAATACTCGGTAGCATTCTCTCAAATATCATTTAGTg                                  <  2:332887/150‑1 (MQ=255)
                                                                                                         tttatAAGTGCTCTATAATTTGAAGGTTCAATTTAAACCGACTAAAAATAACACTGGAAATTATTTTTTGGTTATTTGTTGAGATTTGCTTATGTATTTGTAGTGGTGTTTTCAATACTCGGTAGCATTCTCTCAAATATCATTTAGTGGt                                >  2:131138/1‑151 (MQ=255)
                                                                                                                  gCTCTATAATTTGAAGGTTCAATTTAAACCGACTAAAAATAACACTGGAAATTATTTTTTGGTTATTTGTTGAGATTTGCTTATGTATTTGTAGTGGTGTTTTCAATACTCGGTAGCATTCTCTCAAATATCATTTAGTGGTTtacgtacg                       <  2:344509/151‑1 (MQ=255)
                                                                                                                         aaTTTGAAGGTTCAATTTAAACCGACTAAAAATAACACTGGAAATTATTTTTTGGTTATTTGTTGAGATTTGCTTATGTATTTGTAGTGGTGTTTTCAATACTCGGTAGCATTCTCTCAAATATCATTTAGTGGTTTACGTACGTaaaaa                 >  2:460897/1‑150 (MQ=255)
                                                                                                                           tttGAAGGTTCAATTTAAACCGACTAAAAATAACACTGGAAATTATTTTTTGGTTATTTGTTGAGATTTGCTTATGTATTTGTAGTGGTGTTTTCAATACTCGGTAGCATTCTCTCAAATATCATTTAGTGGTTTACGTACGTAAAAAAtt              >  2:558089/1‑151 (MQ=255)
                                                                                                                            ttGAAGGTTCAATTTAAACCGACTAAAAATAACACTGGAAATTATTTTTTGGTTATTTGTTGAGATTTGCTTATGTATTTGTAGTGGTGTTTTCAATACTCGGTAGCATTCTCTCAAATATCATTTAGTGGTTTACGTACGTAAAAAAtt              <  2:536313/150‑1 (MQ=255)
                                                                                                                             tGAAGGTTCAATTTAAACCGACTAAAAATAACACTGGAAATTATTTTTTGGTTATTTGTTGAGATTTGCTTATGTATTTGTAGTGGTGTTTTCAATACTCGGTAGCATTCTCTCAAATATCATTTAGTGGTTTACGTACGTAAAAAATTgg            >  2:450978/1‑151 (MQ=255)
                                                                                                                             tGAAGGTTCAATTTAAACCGACTAAAAATAACACTGGAAATTATTTTTTGGTTATTTGTTGAGATTTGCTTATGTATTTGTAGTGGTGTTTTCAATACTCGGTAGCATTCTCTCAAATATCATTTAGTGGTTTACGTACGTAAAAAATTgg            >  2:243660/1‑151 (MQ=255)
                                                                                                                                aGGTTCAATTTAAACCGACTAAAAATAACACTGGAAATTATTTTTTGGTTATTTGTTGAGGTTTGCTTATGTATTTGTAGTGGTGTTTTCAATACTCGGTAGCATTCTCTCAAATATCATTTAGTGGTTTACGTACGTAAAAAATTGGTTa         <  1:35236/151‑1 (MQ=255)
                                                                                                                                     cAATTTAAACCGACTAAAAATAACACTGGAAATTATTTTTTGGTTATTTGTTGAGATTTGCTTATGTATTTGTAGTGGTGTTTTCAATACTCGGTAGCATTCTCTCAAATATCATTTAGTGGTTTACGTACGTAAAAAATTGGTTATGCTg    <  2:569505/151‑1 (MQ=255)
                                                                                                                                      aaTTTAAACCGACTAAAAATAACACTGGAAATTATTTTTTGGTTATTTGTTGAGATTTGCTTATGTATTTGTAGTGGTGTTTTCAATACTCGGTAGCATTCTCTCAAATATCATTTAGTGGTTTACGTACGTAAAAAATTGGTTATGCTGt   <  2:498889/151‑1 (MQ=255)
                                                                                                                                       aTTTAAACCGACTAAAAATAACACTGGAAATTATTTTTTGGTTATTTGTTGAGATTTGCTTATGTATTTGTAGTGGTGTTTTCAATACTCGGTAGCATTCTCTCAAATATCATTTAGTGGTTTACGTACGTAAAAAATTGGTTATGCTGtt  >  1:84333/1‑151 (MQ=255)
                                                                                                                                                 |                                                                                                                                            
AAGTAGATCTCTATTTTTATCTGAACTTTTTTCTATCGAATCCTATTCATGGCTCTTGGCTGAATAAAAATAAATCTATTAGCCAATTTATATTAACGGCTGTTATTTATAAGTGCTCTATAATTTGAAGGTTCAATTTAAACCGGCTAAAAATAACACTGGAAATTATTTTTTGGTTATTTGTTGAGATTTGCTTATGTATTTGTAGTGGTGTTTTCAATACTCGGTAGCATTCTCTCAAATATCATTTAGTGGTTTACGTACGTAAAAAATTGGTTATGCTGTT  >  NC_000913/576940‑577225

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑