| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 598,492 | A→G | E338E (GAA→GAG) | cusB → | copper/silver efflux system, membrane fusion protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 598,492 | 0 | A | G | 95.7% | 69.3 / ‑3.2 | 23 | E338E (GAA→GAG) | cusB | copper/silver efflux system, membrane fusion protein |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (15/7); total (16/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01 | |||||||||||
CAGCTGCGTCTGGAAGTCGACAACGCCGACGAGGCGCTAAAACCGGGCATGAACGCCTGGTTGCAACTCAACACCGCCAGCGAACCGATGCTGCTCATTCCGTCACAAGCGCTGATTGATACCGGCAGCGAACAGCGGGTGATTACCGTTGATGCCGACGGGCGCTTTGTACCGAAACGCGTTGCTGTTTTCCAGGCATCGCAAGGCGTCACCGCATTACGCTCTGGTCTGGCGGAAGGTGAAAAGGTGGTTTCCAGCGGCCTGTTCCTG > NC_000913/598361‑598630 | cAGCTGCGTCTGGAAGTCGACAACGCCGACGAGGCGCTAAAACCGGGCATGAACGCCTGGTTGCAACTCAACACCGCCAGCGAACCGATGCTGCTCATTCCGTCACAAGCGCTGATTGATACCGGCAGCGAGCAGCGGGTGATTACCGTTg > 2:338887/1‑151 (MQ=255)cAGCTGCGTCTGGAAGTCGACAACGCCGACGAGGCGCTAAAACCGGGCATGAACGCCTGGTTGCAACTCAACACCGCCAGCGAACCGATGCTGCTCATTCCGTCACAAGCGCTGATTGATACCGGCAGCGAGCAGCGGGTGATTACCGTTg > 2:255906/1‑151 (MQ=255) gCGTCTGGAAGTCGACAACGCCGACGAGGCGCTAAAACCGGGCATGAACGCCTGGTTGCAACTCAACACCGCCAGCGAACCGATGCTGCTCATTCCGTCACAAGCGCTGATTGATACCGGCAGCGAGCAGCGGGTGATTACCGTTGATGcc > 1:217258/1‑151 (MQ=255) gAAGTCGACAACGCCGACGAGGCGCTAAAACCGGGCATGAACGCCTGGTTGCAACTCAACACCGCCAGCGAACCGATGCTGCTCATTCCGTCACAAGCGCTGATTGATACCGGCAGCGAGCAGCGGGTGATTACCGTTGATGCCGACGGgc > 1:471447/1‑151 (MQ=255) gACAACGCCGACGAGGCGCTAAAACCGGGCATGAACGCCTGGTTGCAACTCAACACCGCCAGCGAACCGATGCTGCTCATTCCGTCACAAGCGCTGATTGATACCGGCAGCGAGCAGCGGGTGATTACCGTTGATGCCGACGGGCGCtt < 2:94624/149‑1 (MQ=255) gacgaGGCGCTAAAACCGGGCATGAACGCCTGGTTGCAACTCAACACCGCCAGCGAACCGATGCTGCTCATTCCGTCACAAGCGCTGATTGATACCGGCAGCGAGCAGCGGGTGATTACCGTTGATGCCGACGGGCGCTTTGTACCGAAAc > 1:432718/1‑151 (MQ=255) acgaGGCGCTAAAACCGGGCATGAACGCCTGGTTGCAACTCAACACCGCCAGCGAACCGATGCTGCTCATTCCGTCACAAGCGCTGATTGATACCGGCAGCGAACAGCGGGTGATTACCGTTGATGCCGACGGGCGCTTTGTACCGAAAcg > 1:359004/1‑151 (MQ=255) cgaGGCGCTAAAACCGGGCATGACCGCCTGGTTGCAACTCAACACCGCCAGCGAACCGATGCTGCTCATTCCGTCACAAGCGCTGATTGATACCGGCAGCGAGCAGCGGGTGATTACCGTTGATGCCGACGGGCGCTTTGTACCGaaa < 1:217471/148‑1 (MQ=255) cgaGGCGCTAAAACCGGGCATGAACGCCTGGTTGCAACTCAACACCGCCAGCGAACCGATGCTGCTCATTCCGTCACAAGCGCTGATTGATACCGGCAGCGAGCAGCGGGTGATTACCGTTGATGCCGACGGGCGCTTTGTACCGAAAcg < 2:90008/150‑1 (MQ=255) cTAAAACCGGGCATGAACGCCTGGTTGCAACTCAACACCGCCAGCGAACCGATGCTGCTCATTCCGTCACAAGCGCTGATTGATACCGGCAGCGAGCAGCGGGTGATTACCGTTGATGCCGACGGGCGCTTTGTACCGAAACGCGTTGCTg > 2:77791/1‑151 (MQ=255) aaaaCCGGGCATGAACGCCTGGTTGCAACTCAACACCGCCAGCGAACCGATGCTGCTCATTCCGTCACAAGCGCTGATTGATACCGGCAGCGAGCAGCGGGTGATTACCGTTGATGCCGACGGGCGCTTTGTACCGAAACGCGTTGCTGtt > 1:371052/1‑151 (MQ=255) ccTGGTTGCAACTCAACACCGCCAGCGAACCGATGCTGCTCATTCCGTCACAAGCGCTGATTGATACCGGCAGCGAGCAGCGGGTGATTACCGTTGATGCCGACGGGCGCTTTGTACCGAAACGCGTTGCTGTTTTCCAGGCATCGCAAgg > 2:139290/1‑151 (MQ=255) cTGGTTGCAACTCAACACCGCCAGCGAACCGATGCTGCTCATTCCGTCACAAGCGCTGATTGATACCGGCAGCGAGCAGCGGGTGATTACCGTTGATGCCGACGGGCGCTTTGTACCGAAACGCGTTGCTGTTTTCCAGGCATCGCAAGGc > 2:159005/1‑151 (MQ=255) gTTGCAACTCAACACCGCCAGCGAACCGATGCTGCTCATTCCGTCACAAGCGCTGATTGATACCGGCAGCGAGCAGCGGGTGATTACCGTTGATGCCGACGGGCGCTTTGTACCGAAACGCGTTGCTGTTTTCCAGGCATCGCAAGGCGTc > 2:485627/1‑151 (MQ=255) ttGCAACTCAACACCGCCAGCGAACCGATGCTGCTCATTCCGTCACAAGCGCTGATTGATACCGGCAGCGAGCAGCGGGTGATTACCGTTGATGCCGACGGGCGCTTTGTACCGAAACGCGTTGCTGTTTTCCAGGCATCGCAAGGCGTc > 2:362091/1‑150 (MQ=255) aaCTCAACACCGCCAGCGAACCGATGCTGCTCATTCCGTCACAAGCGCTGATTGATACCGGCAGCGAGCAGCGGGTGATTACCGTTGATGCCGACGGGCGCTTTGTACCGAAACGCGTTGCTGTTTTCCAGGCATCGCAAGGCGTCACCGc < 1:67323/151‑1 (MQ=255) aCTCAACACCGCCAGCGAACCGATGCTGCTCATTCCGTCACAAGCGCTGATTGATACCGGCAGCGAGCAGCGGGTGATTACCGTTGATGCCGACGGGCGCTTTGTACCGAAACGCGTTGCTGTTTTCCAGGCATCGCAAGGCGTCACCGCa < 1:221607/151‑1 (MQ=255) aaCCGATGCTGCTCATTCCGTCACAAGCGCTGATTGATACCGGCAGCGAGCAGCGGGTGATTACCGTTGATGCCGACGGGCGCTTTGTACCGAAACGCGTTGCTGTTTTCCAGGCATCGCAAGGCGTCACCGCATTACGCTCTGGTCTGGc < 2:141293/151‑1 (MQ=255) ctCATTCCGTCACAAGCGCTGATTGATACCGGCAGCGAGCAGCGGGTGATTACCGTTGATGCCGACGGGCGCTTTGTACCGAAACGCGTTGCTGTTTTCCAGGCATCGCAAGGCGTCACCGCATTACGCTCTGGTCTGGCGGAAGGTGaa > 2:404163/1‑150 (MQ=255) tCACAAGCGCTGATTGATACCGGCAGCGAGCAGCGGGTGATTACCGTTGATGCCGACGGGCGCTTTGTACCGAAACGCGTTGCTGTTTTCCAGGCATCGCAAGGCGTCACCGCATTACGCTCTGGTCTGGCGGAAGGTGAAAAGGTGGttt > 2:71160/1‑151 (MQ=255) gattgatACCGGCAGCGAGCAGCGGGTGATTACCGTTGATGCCGACGGGCGCTTTGTACCGAAACGCGTTGCTGTTTTCCAGGCATCGCAAGGCGTCACCGCATTACGCTCTGGTCTGGCGGAAGGTGAAAAGGTGGTTTCCAGCGGCCt < 1:530499/150‑1 (MQ=255) tgatACCGGCAGCGAGCAGCGGGTGATTACCGTTGATGCCGACGGGCGCTTTGTACCGAAACGCGTTGCTGTTTTCCAGGCATCGCAAGGCGTCACCGCATTACGCTCTGGTCTGGCGGAAGGTGAAAAGGTGGTTTCCAGCGGCCTGTTc > 1:297022/1‑151 (MQ=255) aCCGGCAGCGAGCAGCGGGTGATTACCGTTGATGCCGACGGGCGCTTTGTACCGAAACGCGTTGCTGTTTTCCAGGCATCGCAAGGCGTCACCGCATTACGCTCTGGTCTGGCGGAAGGTGAAAAGGTGGTTTCCAGCGGCCTGTTCCtg > 1:441056/1‑150 (MQ=255) | CAGCTGCGTCTGGAAGTCGACAACGCCGACGAGGCGCTAAAACCGGGCATGAACGCCTGGTTGCAACTCAACACCGCCAGCGAACCGATGCTGCTCATTCCGTCACAAGCGCTGATTGATACCGGCAGCGAACAGCGGGTGATTACCGTTGATGCCGACGGGCGCTTTGTACCGAAACGCGTTGCTGTTTTCCAGGCATCGCAAGGCGTCACCGCATTACGCTCTGGTCTGGCGGAAGGTGAAAAGGTGGTTTCCAGCGGCCTGTTCCTG > NC_000913/598361‑598630 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |