Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	628,546	A→G	K285E (AAA→GAA)	entB →	isochorismatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	628,546	0	A	G	100.0%	151.9 / NA	41	K285E (AAA→GAA)	entB	isochorismatase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/22);  total (19/22)

CGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGGCGCTGGCGTTTGTTGAGGCGGGAGC‑GAAAGTTACAGGTTTTGATCAAGCGTTCACTCAGGAGCAATATCCCTTTGCG  >  NC_000913/628410‑628691                                                                                                                                         |                                                                                                                                                   cGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGGAATAATGGATTTCa                                                                                                                                      <  1:36563/151‑1 (MQ=255)  gACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGGAATAATGGATTTc                                                                                                                                       >  1:330496/1‑149 (MQ=255)            tGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGGAATAATGGATTTCAGCGGTAAAAAt                                                                                                                           >  2:99471/1‑151 (MQ=255)              gATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTAATGCTGGCTAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGGAATAATGGATTTCAGCGGTAAAAATGt                                                                                                                         <  2:174137/151‑1 (MQ=255)                  cGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGGAATAATGGATTTCAGCGGTAAAAATGTCTgg                                                                                                                     <  2:586649/151‑1 (MQ=255)                      gcgcATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGGAATAATGGATTTCAGCGGTAAAAATGTCTGGGTaa                                                                                                                 <  1:36526/151‑1 (MQ=255)                       cgcATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGGAATAATGGATTTCAGCGGTAAAAAt                                                                                                                           >  1:567924/1‑140 (MQ=255)                       cgcATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGGAATAATGGATTTCAGCGGTAAAAAt                                                                                                                           <  2:567912/140‑1 (MQ=255)                                gcgcTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGGAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAgg                                                                                                       <  1:356094/151‑1 (MQ=255)                                gcgcTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGGAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAgg                                                                                                       >  2:307686/1‑151 (MQ=255)                                     ggcggcGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGGAATAATGGATTTCAGGGGTAAAAATGTCTGGATAACCGGCGCAGGTaaa                                                                                                   <  2:565429/150‑1 (MQ=255)                                       cggcgCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGGAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGt                                                                                                >  2:587991/1‑151 (MQ=255)                                         gcgcgcTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGGAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTAt                                                                                              <  1:160375/151‑1 (MQ=255)                                         gcgcgcTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGGAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTAt                                                                                              >  1:547230/1‑151 (MQ=255)                                              cTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGGAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCt                                                                                         >  2:160332/1‑151 (MQ=255)                                                     aaaGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGGAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCAc                                                                                  >  1:472112/1‑151 (MQ=255)                                                       aGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGGAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACgg                                                                                <  1:146994/151‑1 (MQ=255)                                                        gTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGGAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGgc                                                                               <  1:160337/151‑1 (MQ=255)                                                            aTGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGGAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGGCGCTg                                                                           <  1:449653/151‑1 (MQ=255)                                                            aTGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGGAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGGCGCTg                                                                           >  2:318229/1‑151 (MQ=255)                                                                 gATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGGAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGGCGCTGGCGtt                                                                      >  1:2527/1‑151 (MQ=255)                                                                    atCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGGAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGGCGCTGGCGTttg                                                                    <  1:288607/150‑1 (MQ=255)                                                                                  gCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGGAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGGCGCTGGCGTTTGTTGAGGCGGGAGC‑g                                                     <  1:479738/150‑1 (MQ=255)                                                                                  gCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGGAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGGCGCTGGCGTTTGTTGAGGCGGGAGC‑Ga                                                    <  1:270496/151‑1 (MQ=255)                                                                                    tGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGGAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGGCGCTGGCGTTTGTTGAGGCGGGAGC‑Gaa                                                   >  2:473347/1‑150 (MQ=255)                                                                                    tGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGGAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGGCGCTGGCGTTTGTTGAGGCGGGAGC‑Gaa                                                   >  1:103174/1‑150 (MQ=255)                                                                                     ggCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGGAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACTGCGCTGGTGTTTTTTGAGGCGGGAGC‑Gaaa                                                  <  2:100151/150‑1 (MQ=255)                                                                                         aaaaaCCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGGAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGGCGCTGGCGTTTGTTGAGGCGGGAGCGGAAAGta                                               >  1:611686/1‑149 (MQ=255)                                                                                            aaCCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGGAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGGCGCTGGCGTTTGTTGAGGCGGGAGC‑GAAAGTTACAgg                                          >  2:389176/1‑151 (MQ=255)                                                                                             aCCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGGAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGGCGCTGGCGTTTGTTGAGGCGGGAGC‑GGAAGTTACAgg                                          >  2:541631/1‑150 (MQ=255)                                                                                             aCCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGGAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGGCGCTGGCGTTTGTTGAGGCGGGAGC‑GGAAGATACCgg                                          >  2:365341/1‑150 (MQ=255)                                                                                              cccGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGGAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGGCGCTGGCGTTTGTTGAGGCGGGGGC‑GAAAGGTACAGGtt                                        >  2:519122/1‑151 (MQ=255)                                                                                              cccGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGGAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGGCGCTGGCGTTTGTTGAGGCGGGAGC‑GAAAGTTACAGGt                                         <  1:594736/150‑1 (MQ=255)                                                                                                     aTCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGGAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGGCGCTGGCGTTTGTTGAGGCGGGAGC‑GGAAGTTAAAGGGTTTGATc                                  >  1:371336/1‑150 (MQ=255)                                                                                                         aCGCCTGGTGGAAGCTACTCTCCCGCGAGGTGGAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGGCGCTGGCGTTTGTTGAGGCGGGAGC‑GAAAGTTACAGGTTTTGATCAAGCg                             <  1:414504/151‑1 (MQ=255)                                                                                                         aCGCCTGGTGGAAGCTACTCTCCCGCGAGGTGGAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGGCGCTGGCGTTTGTTGAGGCGGGAGC‑GAAAGTTACAGGTTTTGATCAAGCg                             <  2:84859/151‑1 (MQ=255)                                                                                                                gtggAAGCTACTCTCCCGCGAGGTGGAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGGCGCTGGCGTTTGTTGAGGCGGGAGC‑GGAAGTTACAGGGTTTGATCAAGCGGTCACTc                      >  1:191150/1‑151 (MQ=255)                                                                                                                    aaGCTACTCTCCCGCGAGGTGGAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGGCGCTGGCGTTTGTTGAGGCGGGAGC‑GAAAGTTACAGGTTTTGATCAAGCGTTCACTCAGGa                  <  2:543567/151‑1 (MQ=255)                                                                                                                              cccGCGAGGTGGAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGGCGCTGGCGTTTGTTGAGGCGGGAGC‑GAAAGTTACAGGTTTTGATCAAGCGTTCACTCAGGAGCAatat           <  2:459756/148‑1 (MQ=255)                                                                                                                                   gAGGTGGAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGGCGCTGGCGTTTGTTGAGGCGGGAGC‑GAAAGTTACAGGTTTTGATCAAGCGTTCACTCAGGAGCAATATCCCTTTGc   <  1:403004/151‑1 (MQ=255)                                                                                                                                    aGGTGGAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGGCGCTGGCGTTTGTTGAGGCGGGAGC‑GAAAGTTACAGGTTTTGATCAAGCGTTCACTCAGGAGCAATATCCCTTTGCg  <  1:242558/151‑1 (MQ=255)                                                                                                                                         |                                                                                                                                                   CGACTACGGTCTGGATTCGGTGCGCATGATGGCGCTGGCGGCGCGCTGGCGCAAAGTGCATGGTGATATCGACTTTGTCATGCTGGCGAAAAACCCGACCATCGACGCCTGGTGGAAGCTACTCTCCCGCGAGGTGAAATAATGGATTTCAGCGGTAAAAATGTCTGGGTAACCGGCGCAGGTAAAGGTATCGGCTACGCCACGGCGCTGGCGTTTGTTGAGGCGGGAGC‑GAAAGTTACAGGTTTTGATCAAGCGTTCACTCAGGAGCAATATCCCTTTGCG  >  NC_000913/628410‑628691

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑