Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	73,145	T→C	K459K (AAA→AAG)	thiP ←	thiamine/thiamine pyrophosphate ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	73,145	0	T	C	100.0%	119.3 / NA	33	K459K (AAA→AAG)	thiP	thiamine/thiamine pyrophosphate ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/20);  total (13/20)

GCACCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACAGCACGCATGCAAAGGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCCACCACTTTTAAGCGTGACCAGCCTTCAATCCCCAGCGACTGACATAACATGCTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGCACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTG  >  NC_000913/72999‑73292                                                                                                                                                   |                                                                                                                                                    gCACCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACAGCACGCATGCAAAGGCCAGCGCCTGCGCCAGTGGACGCTTc                                                                                                                                                  >  2:289084/1‑150 (MQ=255) gCACCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACAGCACGCATGCAAAGGCCAGCGCCTGCGCCAGTGGACGCTTCa                                                                                                                                                 <  2:37993/151‑1 (MQ=255) gCACCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACAGCACGCATGCAAAGGCCAGCGCCTGCGCCAGTGGACGCTTCa                                                                                                                                                 <  2:22398/151‑1 (MQ=255)    ccGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACAGCACGCATGCAAAGGCCAGCGCCTGCGCCAGTGGACGCTTCAggg                                                                                                                                              >  2:107834/1‑151 (MQ=255)         cTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACAGCACGCATGCAAAGGCCAGCGCCTGCGCCAGTGGACGCTTCAGGgcgcgc                                                                                                                                         >  2:274279/1‑151 (MQ=255)                     ggAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACAGCACGCATGCAAAGGCCAGCGCCTGCGCCAGTGGACGCTTCAGGGCGCGCAGCTCCACCACt                                                                                                                             <  1:252340/151‑1 (MQ=255)                        gCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACAGCACGCATGCAAAGGCCAGCGCCTGCGCCAGTGGACGCTTCAGGGCGCGCAGCTCCACCACtttt                                                                                                                          <  2:281437/151‑1 (MQ=255)                        gCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACAGCACGCATGCAAAGGCCAGCGCCTGCGCCAGTGGACGCTTCAGGGCGCGCAGCTCCACCACtttt                                                                                                                          <  1:632422/151‑1 (MQ=255)                         ccAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACAGCACGCATGCAAAGGCCAGCGCCTGCGCCAGTGGACGCTTCAGGGCGCGCAGCTCCACCACtttt                                                                                                                          >  1:10462/1‑150 (MQ=255)                                   ggTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACAGCACGCATGCAAAGGCCAGCGCCTGCGCCAGTGGACGCTTCAGGGCGCGCAGCTCCACCACTTTTAAGCGTGAcc                                                                                                                <  1:385262/150‑1 (MQ=255)                                                   cAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACAGCACGCATGCAAAGGCCAGCGCCTGCGCCAGTGGACGCTTCAGGGCGCGCAGCTCCACCACTTTTAAGCGTGACCAGCCTTCAATCCCCAGc                                                                                               <  2:94141/151‑1 (MQ=255)                                                    aGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACAGCACGCATGCAAAGGCCAGCGCCTGCGCCAGTGGACGCTTCAGGGCGCGCAGCTCCACCACTTTTAAGCGTGACCAGCCTTCAATCCCCAGCg                                                                                              <  1:346944/151‑1 (MQ=255)                                                         gCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACAGCACGCATGCAAAGGCCAGCGCCTGCGCCAGTGGACGCTTCAGGGCGCGCAGCTCCACCACTTTTAAGCGTGACCAGCCTTCAATCCCCAGCGACTg                                                                                          >  2:331986/1‑150 (MQ=255)                                                                        aCCGAACAACGCCACCACGCCAAAATCACCAATCGACAGCACGCATGCAAAGGCCAGCGCCTGCGCCAGTGGACGCTTCAGGGCGCGCAGCTCCACCACTTTTAAGCGTGACCAGCCTTCAATCCCCAGCGACTGACATAACATGCTGt                                                                            >  1:362820/1‑149 (MQ=255)                                                                        aCCGAACAACGCCACCACGCCAAAATCACCAATCGACAGCACGCATGCAAAGGCCAGCGCCTGCGCCAGTGGACGCTTCAGGGCGCGCAGCTCCACCACTTTTAAGCGTGACCAGCCTTCAATCCCCAGCGACTGACATAACATGCTGTAg                                                                          <  2:319423/151‑1 (MQ=255)                                                                           gAACAACGCCACCACGCCAAAATCACCAATCGACAGCACGCATGCAAAGGCCAGCGCCTGCGCCAGTGGACGCTTCAGGGCGCGCAGCTCCACCACTTTTAAGCGTGACCAGCCTTCAATCCCCAGCGACTGACATAACATGCTGTAgcgg                                                                       <  1:120308/151‑1 (MQ=255)                                                                            aacaacGCCACCACGCCAAAATCACCAATCGACAGCACGCATGCAAAGGCCAGCGCCTGCGCCAGTGGACGCTTCAGGGCGCGCAGCTCCACCTCTTTTAAGCGTGACCAGCCTTCAATCCCCAGCGACTGACATAACATGCTGTAgcggg                                                                      <  1:489593/151‑1 (MQ=255)                                                                                      ccacGCCAAAATCACCAATCGACAGCACGCATGCAAAGGCCAGCGCCTGCGCCAGTGGACGCTTCAGGGCGCGCAGCTCCACCACTTTTAAGCGTGACCAGCCTTCAATCCCCAGCGACTGACATAACATGCTGTAGCGGGCGGTGatat                                                             >  2:504187/1‑150 (MQ=255)                                                                                            cAAAATCACCAATCGACAGCACGCATGCAAAGGCCAGCGCCTGCGCCAGTGGACGCTTCAGGGCGCGCAGCTCCACCACTTTTAAGCGTGACCAGCCTTCAATCCCCAGCGACTGACATAACATGCTGTAGCGGGCGGTGATATCGCGCAt                                                      <  2:99788/151‑1 (MQ=255)                                                                                             aaaaTCACCAATCGACAGCACGCATGCAAAGGCCAGCGCCTGCGCCAGTGGACGCTTCAGGGCGCGCAGCTCCACCACTTTTAAGCGTGACCAGCCTTCAATCCCCAGCGACTGACATAACATGCTGTAGCGGGCGGTGATATCGCGCATc                                                     >  2:348328/1‑151 (MQ=255)                                                                                              aaaTCACCAATCGACAGCACGCATGCAAAGGCCAGCGCCTGCGCCAGTGGACGCTTCAGGGCGCGCAGCTCCACCACTTTTAAGCGTGACCAGCCTTCAATCCCCAGCGACTGACATAACATGCTGTAGCGGGCGGTGATATCGCGCATCg                                                    <  1:466770/151‑1 (MQ=255)                                                                                                  cACCAATCGACAGCACGCATGCAAAGGCCAGCGCCTGCGCCAGTGGACGCTTCAGGGCGCGCAGCTCCACCACTTTTAAGCGTGACCAGCCTTCAATCCCCAGCGACTGACATAACATGCTGTAGCGGGCGGTGATATCGCGCATCGGGt                                                 <  1:218489/150‑1 (MQ=255)                                                                                                      aaTCGACAGCACGCATGCAAAGGCCAGCGCCTGCGCCAGTGGACGCTTCAGGGCGCGCAGCTCCACCACTTTTAAGCGTGACCAGCCTTCAATCCCCAGCGACTGACATAACATGCTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTcc                                            >  1:8558/1‑151 (MQ=255)                                                                                                       aTCGACAGCACGCATGCAAAGGCCAGCGCCTGCGCCAGTGGACGCTTCAGGGCGCGCAGCTCCACCACTTTTAAGCGTGACCAGCCTTCAATCCCCAGCGACTGACATAACATGCTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCa                                           <  1:348335/151‑1 (MQ=255)                                                                                                         cGACAGCACGCATGCAAAGGCCAGCGCCTGCGCCAGTGGACGCTTCAGGGCGCGCAGCTCCACCACTTTTAAGCGTGACCAGCCTTCAATCCCCAGCGACTGACATAACATGCTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGc                                         <  2:257937/151‑1 (MQ=255)                                                                                                                    aTGCAAAGGCCAGCGCCTGCGCCAGTGGACGCTTCAGGGCGCGCAGCTCCACCACTTTTAAGCGTGACCAGCCTTCAATCCCCAGCGACTGACATAACATGCTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGCACTTTCAgcgc                              >  1:553886/1‑151 (MQ=255)                                                                                                                        aaaGGCCAGCGCCTGCGCCAGTGGACGCTTCAGGGCGCGCAGCTCCACCACTTTTAAGCGTGACCAGCCTTCAATCCCCAGCGACTGACATAACATGCTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGCACTTTCAGCGCATa                           >  1:302915/1‑150 (MQ=255)                                                                                                                        aaaGGCCAGAGCCTGCGCCAGTGGACGCTTCAGGGCGCGCAGCTCCACCACTTTTAAGCGTGACCAGCCTTCAATCCCCAGCGACTGACATAACATGCTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGCACTTTCAGCGCATa                           >  2:629622/1‑150 (MQ=255)                                                                                                                          aGGCCAGCGCCTGCGCCAGTGGACGCTTCAGGGCGCGCAGCTCCACCACTTTTAAGCGTGACCAGCCTTCAATCCCCAGCGACTGACATAACATGCTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGCACTTTCAGCGCATAAgg                        <  1:278377/151‑1 (MQ=255)                                                                                                                            gCCAGCGCCTGCGCCAGTGGACGCTTCAGGGCGCGCAGCTCCACCACTTTTAAGCGTGACCAGCCTTCAATCCCCAGCGACTGACATAACATGCTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGCACTTTCAGCGCATAAGGGa                      <  2:267554/151‑1 (MQ=255)                                                                                                                                 cgcCTGCGCCAGTGGACGCTTCAGGGCGCGCAGCTCCACCACTTTTAAGCGTGACCAGCCTTCAATCCCCAGCGACTGACATAACATGCTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGCACTTTCAGCGCATAAGGGATCGcc                 <  2:614043/151‑1 (MQ=255)                                                                                                                                        gcCAGTGGACGCTTCAGGGCGCGCAGCTCCACCACTTTTAAGCGTGACCAGCCTTCAATCCCCAGCGACTGACATAACATGCTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGCACTTTCAGCGCATAAGGGATCGCCATTAACg          >  1:337351/1‑151 (MQ=255)                                                                                                                                                aCGCTTCAGGGCGCGCAGCTCCACCACTTTTAAGCGTGACCAGCCTTCAATCCCCAGCGACTGACATAACATGCTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGCACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTg  <  2:292021/151‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                    GCACCGTCCTGGCTGCGATAGGAGCCAATTTGCTGGTAGAGATAAAACGGCAGGGTGCGGAAATCATCGTTACCGAACAACGCCACCACGCCAAAATCACCAATCGACAGCACGCATGCAAAGGCCAGCGCCTGCGCCAGTGGACGTTTCAGGGCGCGCAGCTCCACCACTTTTAAGCGTGACCAGCCTTCAATCCCCAGCGACTGACATAACATGCTGTAGCGGGCGGTGATATCGCGCATCGGGTTTTCCAGCACTTTCAGCGCATAAGGGATCGCCATTAACGCATTGGTG  >  NC_000913/72999‑73292

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑