Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	694,122	G→A	G278G (GGC→GGT)	miaB ←	tRNA‑i(6)A37 methylthiotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	694,122	0	G	A	100.0%	161.1 / NA	41	G278G (GGC→GGT)	miaB	tRNA‑i(6)A37 methylthiotransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/28);  total (13/28)

CAATCAGCTTCATCGTTTTCTCGAAGTCTTCGGTGGTTTCGCCAGGGAAGCCAACGATGAAATCAGAACTGATCTGAATATCTGGACGCGCCGCACGCAGTTTACGGATGATCGCTTTGTACTCCAGCGCCGTATGGGTACGGCCCATCAGGTTCAGAATGCGATCGGAACCGCTCTGTACCGGCAGATGCAGGAAGCTCACCAGCTCCGGCGTGTCGCGATACACTTCGATGATATCGTCGGTGAATTCGATCGGATGGCTGGTGGTAAAGCGAATACGATCGATCC  >  NC_000913/693980‑694267                                                                                                                                               |                                                                                                                                                  cAATCAGCTTCATCGTTTTCTCGAAGTCTTCGGTGGTTTCGCCAGGGAAGCCAACGATGAAATCAGAACTGATCTGAATATCTGGACGCGCCGCACGCAGTTTACGGATGATCGCTTTGTACTCCAGCGCCGTATGGGTACGACCCATCAg                                                                                                                                           <  1:306770/151‑1 (MQ=255)     cAGCTTCATCGTTTTCTCGAAGTCTTCGGTGGTTTCGCCAGGGAAGCCAACGATGAAATCAGAACTGATCTGAATATCTGGACGCGCCGCACGCAGTTTACGGATGATCGCTTTGTACTCCAGCGCCGTATGGGTACGACCCATCAGGTTc                                                                                                                                       <  2:433166/151‑1 (MQ=255)      aGCTTCATCGTTTTCTCGAAGTCTTCGGTGGTTTCGCCAGGGAAGCCAACGATGAAATCAGAACTGATCTGAATATCTGGACGCGCCGCACGCAGTTTACGGATGATCGCTTTGTACTCCAGCGCCGTATGGGTACGACCCATCAGGTTc                                                                                                                                       >  2:582937/1‑150 (MQ=255)          tCATCGTTTTCTCGAAGTCTTCGGTGGTTTCGCCAGGGAAGCCAACGATGAAATCAGAACTGATCTGAATATCTGGACGCGCCGCACGCAGTTTACGGATGATCGCTTTGTACTCCAGCGCCGTATGGGTACGACCCATCAGGTTCAGaa                                                                                                                                   <  1:60825/150‑1 (MQ=255)           cATCGTTTTCTCGAAGTCTTCGGTGGTTTCGCCAGGGAAGCCAACGATGAAATCAGAACTGATCTGAATATCTGGACGCGCCGCACGCAGTTTACGGATGATCGCTTTGTACTCCAGCGCCGTATGGGTACGACCCATCAGGTTCAGAATg                                                                                                                                 <  2:382325/151‑1 (MQ=255)                ttttCTCGAAGTCTTCGGTGGTTTCGCCAGGGAAGCCAACGATGAAATCAGAACTGATCTGAATATCTGGACGCGCCGCACGCAGTTTACGGATGATCGCTTTGTACTCCAGCGCCGTATGGGTACGACCCATCAGGTTCAGAATGCGATc                                                                                                                            >  1:306038/1‑151 (MQ=255)                 tttCTCGAAGTCTTCGGTGGTTTCGCCAGGGAAGCCAACGATGAAATCAGAACTGATCTGAATATCTGGACGCGCCGCACGCAGTTTACGGATGATCGCTTTGTACTCCAGCGCCGTATGGGTACGACCCATCAGGTTCAGAATGCGATc                                                                                                                            <  1:212067/150‑1 (MQ=255)                   tctcGAAGTCTTCGGTGGTTTCGCCAGGGAAGCCAACGATGAAATCAGAACTGATCTGAATATCTGGACGCGCCGCACGCAGTTTACGGATGATCGCTTTGTACTCCAGCGCCGTATGGGTACGACCCATCAGGTTCAGAATGCGATCgg                                                                                                                          >  2:546004/1‑150 (MQ=255)                     tcGAAGTCTTCGGTGGTTTCGCCAGGGAAGCCAACGATGAAATCAGAACTGATCTGAATATCTGGACGCGCCGCACGCAGTTTACGGATGATCGCTTTGTACTCCAGCGCCGTATGGGTACGACCCATCAGGTTCAGAATGCGATCGGAAc                                                                                                                       >  1:35171/1‑151 (MQ=255)                      cGAAGTCTTCGGTGGTTTCGCCAGGGAAGCCAACGATGAAATCAGAACTGATCTGAATATCTGGACGCGCCGCACGCAGTTTACGGATGATCGCTTTGTACTCCAGCGCCGTATGGGTACGACCCATCAGGTTCAGAATGCGATCGGa                                                                                                                         <  1:291963/148‑1 (MQ=255)                                   ggtTTCGCCAGGGAAGCCAACGATGAAATCAGAACTGATCTGAATATCTGGACGCGCCGCACGCAGTTTACGGATGATCGCTTTGTACTCCAGCGCCGTATGGGTACGACCCATCAGGTTCAGAATGCGATCGGAACCGCTCTGTACCgg                                                                                                          <  1:316611/150‑1 (MQ=255)                                       tCGCCAGGGAAGCCAACGATGAAATCAGAACTGATCTGAATATCTGGACGCGCCGCACGCAGTTTACGGATGATCGCTTTGTACTCCAGCGCCGTATGGGTACGACCCATCAGGTTCAGAATGCGATCGGAACCGCTCTGTACCGGc                                                                                                         >  1:344385/1‑147 (MQ=255)                                         gCCAGGGAAGCCAACGATGAAATCAGAACTGATCTGAATATCTGGACGCGCCGCACGCAGTTTACGGATGATCGCTTTGTACTCCAGCGCCGTATGGGTACGACCCATCAGGTTCAGAATGCGATCGGAACCGCTCTGTACCGGCAGATGc                                                                                                   >  2:259480/1‑151 (MQ=255)                                           cAGGGAAGCCAACGATGAAATCAGAACTGATCTGAATATCTGGACGCGCCGCACGCAGTTTACGGATGATCGCTTTGTACTCCAGCGCCGTATGGGTACGACCCATCAGGTTCAGAATGCGATCGGAACCGCTCTGTACCGGCAGATGCAg                                                                                                 <  1:326685/151‑1 (MQ=255)                                               gAAGCCAACGATGAAATCAGAACTGATCTGAATATCTGGACGCGCCGCACGCAGTTTACGGATGATCGCTTTGTACTCCAGCGCCGTATGGGTACGACCCATCAGGTTCAGAATGCGATCGGAACCGCTCTGTACCGGCAGATGCAGGa                                                                                               >  1:229807/1‑149 (MQ=255)                                                      aCGATGAAATCAGAACTGATCTGAATATCTGGACGCGCCGCACGCAGTTTACGGATGATCGCTTTGTACTCCAGCGCCGTATGGGTACGACCCATCAGGTTCAGAATGCGATCGGAACCGCTCTGTACCGGCAGATGCAGGAAGCTCAcc                                                                                       >  1:18344/1‑150 (MQ=255)                                                      aCGATGAAATCAGAACTGATCTGAATATCTGGACGCGCCGCACGCAGTTTACGGATGATCGCTTTGTACTCCAGCGCCGTATGGGTACGACCCATCAGGTTCAGAATGCGATCGGAACCGCTCTGTACCGGCAGATGCAGGAAGCTCACCa                                                                                      <  1:199224/151‑1 (MQ=255)                                                             aaTCAGAACTGATCTGAATATCTGGACGCGCCGCACGCAGTTTACGGATGATCGCTTTGTACTCCAGCGCCGTATGGGTACGACCCATCAGGTTCAGAATGCGATCGGAACCGCTCTGTACCGGCAGATGCAGGAAGCTCACCAGCTCCgg                                                                               >  1:29633/1‑151 (MQ=255)                                                                    aCTGATCTGAATATCTGGACGCGCCGCACGCAGTTTACGGATGATCGCTTTGTACTCCAGCGCCGTATGGGTACGACCCATCAGGTTCAGAATGCGATCGGAACCGCTCTGTACCGGCAGATGCAGGAAGCTCACCAGCTCCGGCGTGTcg                                                                        <  1:380050/151‑1 (MQ=255)                                                                    aCTGATCTGAATACCTGGCCCCGCCGCACTCTGTTTACGGATGATCGCTTTGTACTCCAGCGCCGTATGGGTACGACCCATCAGGTTCAGAATGCGATCGGAACCGCTCTGTACCGGCAAATGCAGGTAGCCGACCAGCTCCGGCGTGTcg                                                                        <  2:213564/151‑1 (MQ=255)                                                                       gATCTGAATATCTGGACGCGCCGCACGCAGTTTACGGATGATCGCTTTGTACTCCAGCGCCGTATGGGTACGACCCATCAGGTTCAGAATGCGATCGGAACCGCTCTGTACCGGCAGATGCAGGAAGCTCACCAGCTCCGGCGTGTcgc                                                                       <  1:293576/149‑1 (MQ=255)                                                                          cTGAATATCTGGACGCGCCGCACGCAGTTTACGGATGATCGCTTTGTACTCCAGCGCCGTATGGGTACGACCCATCAGGTTCAGAATGCGATCGGAACCGCTCTGTACCGGCAGATGCAGGAAGCTCACCAGCTCCGGCGTGTCGCGATac                                                                  >  2:187776/1‑151 (MQ=255)                                                                             aaTATCTGGACGCGCCGCACGCAGTTTACGGATGATCGCTTTGTACTCCAGCGCCGTATGGGTACGACCCATCAGGTTCAGAATGCGATCGGAACCGCTCTGTACCGGCAGATGCAGGAAGCTCACCAGCTCCGGCGTGTCGCGATACACt                                                               <  2:248306/151‑1 (MQ=255)                                                                                       cgcgCCGCACGCAGTTTACGGATGATCGCTTTGTACTCCAGCGCCGTATGGGTACGACCCATCAGGTTCAGAATGCGATCGGAACCGCTCTGTACCGGCAGATGCAGGAAGCTCACCAGCTCCGGCGTGTCGCGATACACTTCGATGata                                                      <  2:352037/150‑1 (MQ=255)                                                                                        gcgcCGCACGCAGTTTACGGATGATCGCTTTGTACTCCAGCGCCGTATGGGTACGACCCATCAGGTTCAGAATGCGATCGGAACCGCTCTGTACCGGCAGATGCAGGAAGCTCACCAGCTCCGGCGTGTCGCGATACACTTCGATGATAtc                                                    <  1:377705/151‑1 (MQ=255)                                                                                             gcacgcaGTTTACGGATGATCGCTTTGTACTCCAGCGCCGTATGGGTACGACCCATCAGGTTCAGAATGCGATCGGAACCGCTCTGTACCGGCAGATGCAGGAAGCTCACCAGCTCCGGCGTGTCGCGATACACTTCGATGATAtcgtcg                                                <  2:237828/150‑1 (MQ=255)                                                                                               acgcaGTTTACGGATGATCGCTTTGTACTCCAGCGCCGTATGGGTACGACCCATCAGGTTCAGAATGCGATCGGAACCGCTCTGTACCGGCAGATGCAGGAAGCTCACCAGCTCCGGCGTGTCGCGATACACTTCGATGatat                                                     <  2:87816/143‑1 (MQ=255)                                                                                               acgcaGTTTACGGATGATCGCTTTGTACTCCAGCGCCGTATGGGTACGACCCATCAGGTTCAGAATGCGATCGGAACCGCTCTGTACCGGCAGATGCAGGAAGCTCACCAGCTCCGGCGTGTCGCGATACACTTCGATGatat                                                     >  1:87816/1‑143 (MQ=255)                                                                                                  caGTTTACGGATGATCGCTTTGTACTCCAGCGCCGTATGGGTACGACCCATCAGGTTCAGAATGCGATCGGAACCGCTCTGTACCGGCACATGCAGGAAGCTCACCAGCTCCGGCGTGTCGCGATACACTTCGATGATATCGTCGGTGAAt                                          >  1:225134/1‑151 (MQ=255)                                                                                                         cGGATGATCGCTTTGTACTCCAGCGCCGTATGGGTACGACCCATCAGGTTCAGAATGCGATCGGAACCGCTCTGTACCGGCAGATGCAGGAAGCTCACCAGCTCTGGCGTGTCGCGATACACTTCGATGATATCGTCGGTGAATTCGATCg                                   <  2:422429/151‑1 (MQ=255)                                                                                                          ggATGATCGCTTTGTACTCCAGCGCCGTATGGGTACGACCCATCAGGTTCAGAATGCGATCGGAACCGCTCTGTACCGGCAGATGCAGGAAGCTCACCAGCTCCGGCGTGTCGCGATACACTTCGATGATATCGTCGGTGAATTCGATCgg                                  >  2:594271/1‑151 (MQ=255)                                                                                                            atgatCGCTTTGTACTCCAGCGCCGTATGGGTACGACCCATCAGGTTCAGAATGCGATCGGAACCGCTCTGTACCGGCAGATGCAGGAAGCTCACCAGCTCCGGCGTGTCGCGATACACTTCGATGATATCGTCGGTGAATTCGATCGGAt                                <  1:480907/151‑1 (MQ=255)                                                                                                                  gCTTTGTACTCCAGCGCCGTATGGGTACGACCCATCAGGTTCAGAATGCGATCGGAACCGCTCTGTACCGGCAGATGCAGGAAGCTCACCAGCTCCGGCGTGTCGCGATACACTTCGATGATATCGTCGGTGAATTCGATCGGATGGCtgg                          <  1:360976/151‑1 (MQ=255)                                                                                                                        tACTCCAGCGCCGTATGGGTACGACCCATCAGGTTCAGAATGCGATCGGAACCGCTCTGTACCGGCAGATGCAGGAAGCTCACCAGCTCCGGCGTGTCGCGATACACTTCGATGATATCGTCGGTGAATTCGATCGGATGGCTGGTGGTa                     <  1:101345/150‑1 (MQ=255)                                                                                                                         aCTCCAGCGCCGTATGGGTACGACCCATCAGGTTCAGAATGCGATCGGAACCGCTCTGTACCGGCAGATGCAGGAAGCTCACCAGCTCCGGCGTGTCGCGATACACTTCGATGATATCGTCGGTGAATTCGATCGGATGGCTGGTGGTaaa                   <  2:470925/151‑1 (MQ=255)                                                                                                                         aCTCCAGCGCCGTATGGGTACGACCCATCAGGTTCAGAATGCGATCGGAACCGCTCTGTACCGGCAGATGCAGGAAGCTCACCAGCTCCGGCGTGTCGCGATACACTTCGATGATATCGTCGGTGAATTCGATCGGATGGCTGGTGGTaa                    <  2:182337/150‑1 (MQ=255)                                                                                                                            ccAGCGCCGTATGGGTACGACCCATCAGGTTCAGAATGCGATCGGAACCGCTCTGTACCGGCAGATGCAGGAAGCTCACCAGCTCCGGCGTGTCGCGATACACTTCGATGATATCGTCGGTGAATTCGATCGGATGGCTGGTGGTAAAg                  <  1:476813/149‑1 (MQ=255)                                                                                                                             cAGCGCCGTATGGGTACGACCCATCAGGTTCAGAATGCGATCGGAACCGCTCTGTACCGGCACATGCAGGAAGCTCACCAGCTCCGGCGTGTCGCGATACACTTCGATGATATCGTCGGTGAATTCGATCGGATGGCTGGTGGTAAAGCGa               <  2:225129/151‑1 (MQ=255)                                                                                                                               gcgcCGTATGGGTACGACCCATCAGGTTCAGAATGCGATCGGAACCGCTCTGTACCGGCAGATGCAGGAAGCTCACCAGCTCCGGCGTGTCGCGATACACTTCGATGATATCGTCGGTGAATTCGATCGGATGGCTGGTGGTAAAGCGAAt             <  1:134655/151‑1 (MQ=255)                                                                                                                                      aTGGGTACGACCCATCAGGTTCAGAATGCGATCGGAACCGCTCTGTACCGGCAGATGCAGGAAGCTCACCAGCTCCGGCGTGTCGCGATACACTTCGATGATATCGTCGGTGAATTCGATCGGATGGCTGGTGGTAAAGCGAATAcgatc       <  2:541634/150‑1 (MQ=255)                                                                                                                                          gTACGACCCATCAGGTTCAGAATGCGATCGGAACCGCTCTGTACCGGCAGATGCAGGAAGCTCACCAGCTCCGGCGTGTCGCGATACACTTCGATGATATCGTCGGTGAATTCGATCGGATGGCTGGTGGTAAAGCGAATACGATCGATcc  <  2:306031/151‑1 (MQ=255)                                                                                                                                               |                                                                                                                                                  CAATCAGCTTCATCGTTTTCTCGAAGTCTTCGGTGGTTTCGCCAGGGAAGCCAACGATGAAATCAGAACTGATCTGAATATCTGGACGCGCCGCACGCAGTTTACGGATGATCGCTTTGTACTCCAGCGCCGTATGGGTACGGCCCATCAGGTTCAGAATGCGATCGGAACCGCTCTGTACCGGCAGATGCAGGAAGCTCACCAGCTCCGGCGTGTCGCGATACACTTCGATGATATCGTCGGTGAATTCGATCGGATGGCTGGTGGTAAAGCGAATACGATCGATCC  >  NC_000913/693980‑694267

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑